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Applied Genetics
Applied Genetics

... organism with the DNA of another organism. • Recombinant DNA technology was first used in the 1970’s with bacteria. ...
Practice Science Olympiad Exam: Designer Genes
Practice Science Olympiad Exam: Designer Genes

... 18. What is the “backbone” of DNA made from and what type of bonds does the element share with the adjacent sugars? 19. What is the protein called that connect two chromatids to form a chromosome? 20. What protein does DNA strands wrap around when coiling, and how many times does it wrap around the ...
DNA Replication
DNA Replication

...  The tips of chromosomes are known as telomeres ...
Slide 1
Slide 1

... There are 3 major differences between RNA and DNA. The sugar in RNA is ribose, not deoxyribose. RNA consists of a single strand of nucleotides, and DNA is double-stranded. The nitrogenous bases in RNA are different than DNA. RNA contains: Adenine ...
Mutations - Choteau Schools
Mutations - Choteau Schools

... Chromosomal Mutations Very common in plants. During meiosis the homologous chromosomes will not pair correctly when one chromosome has extra or missing parts, resulting in incorrect separation of the homologous ...
528 MISCELLANEOUS METHODS [32] [32] An Agarose Gel
528 MISCELLANEOUS METHODS [32] [32] An Agarose Gel

C1. At the molecular level, sister chromatid exchange and
C1. At the molecular level, sister chromatid exchange and

... chromosome. It brings the l DNA close to the chromosome and then makes staggered cuts in the attachment sites. The strands are exchanged, and then integrase catalyzes the covalent attachment of the strands to each other. In this way, the l DNA is inserted at a precise location within the E. coli chr ...
Chapter 13 Genetic Engineering - Mrs. Moyer
Chapter 13 Genetic Engineering - Mrs. Moyer

... can synthesize a DNA strand and connect it to a circular DNA molecule known as a plasmid… which can be found naturally in bacteria. This bacteria can then be injected into a plant, and will insert its DNA into the plant. ► If transformation is successful, the recombinant DNA is integrated into one o ...
Chapter 16 notes
Chapter 16 notes

... Results: radioactivity in supernatant, therefore, protein did not enter the bacteria ...
File
File

... Recombinant DNA is made by taking short pieces of DNA from one organism and joining it to the DNA of a completely different organism. Once the DNA is made, it can be placed back into a living cell in a process called transformation. ...
Competency Goal 2: The learner will develop an understanding of
Competency Goal 2: The learner will develop an understanding of

... Goal 3 Key Concepts Review: The learner will develop an understanding of the continuity of life and the changes of organisms over time. 1. Define DNA and give its function. (Ch 12) 2. What is the shape of DNA? (293) 3. Who discovered the structure of DNA in 1953? (293) 4. What are the three main par ...
electroporation of a - The Steve Clough Lab
electroporation of a - The Steve Clough Lab

DNA damage (Comet Assay) as biomarker of Cd exposure in
DNA damage (Comet Assay) as biomarker of Cd exposure in

... Copper oxide (CuO) is one of the most widely used nanoparticle applications in consumer products. They are extensively used in microelectronics, cosmetics and catalysts. In the present study, the DNA damaging potential of CuO-NPs in the marine eastern mussel Mytilus trossulus was evaluated and compa ...
Sample question
Sample question

... B. DNA protects the cell from invaders C. DNA speeds up chemical reactions D. DNA determines what proteins are made Question #2: ...
EXAM 2
EXAM 2

... 35. Any change in the chemical composition of DNA is a _mutation______________. 36. An alteration in the DNA composition that is not passed on to the subsequent genereation is referred to as _somatic______________, while those that can be passed on are referred to as _gametic___________. 37. An alte ...
Recombinant DNA Technology (b)
Recombinant DNA Technology (b)

... • Many waste products of agriculture/industry do not break down naturally/break down slowly. Many bacteria have been GE capable of breaking down oil and other organic wastes in Cheese making industry : GE Saccharomyces cerevisiae able to dispose of whey by converting lactose to alcohol. Agricultural ...
The Genetic Code and Transcription Chapter 12 Honors Genetics
The Genetic Code and Transcription Chapter 12 Honors Genetics

... • Each “word” in the mRNA strand is composed of a 3-letter sequence called a CODON. • Each CODON specifies a SINGLE Amino Acid. • There is 1 start codon for initiation of protein synthesis and 3 stop codons for ending protein synthesis for a specific protein. • A given amino acid can have more than ...
Lecture 5 The chemical nature of the Gene
Lecture 5 The chemical nature of the Gene

... (1) Evidence that Genes are located on Chromosomes 1902 – McClung – a particular chromosome (X) determines sex in insects (XO = male; XX = female) 1903 – Sutton and Boveri – chromosomes behave just like the unit factors described by Mendel 1910 – Morgan – the white eye color gene of Drosophila is lo ...
A. Restriction Enzymes
A. Restriction Enzymes

... http://www.youtube.com/watch?v=TpmNfv1jKuA ...
Making Copies of DNA
Making Copies of DNA

... Double helix structure ➔ Phosphate + sugar backbone ➔ Paired bases in the middle (must be correctly bonded base pairs) ...
Restriction Enzyme
Restriction Enzyme

... 2. Forensics - Some genes are highly variable within a population (human leukocyte antigen type, HLA) 3. Molecular Evolution - DNA is very stable and remain intact for thousands of years or longer, particularly when shield from air, light and water ...
Wanganui High School
Wanganui High School

... cystic fibrosis: disease caused by a recessive gene causes breathing problems due to mucus forming in the breathing system diploid: cell with the full complement of chromosomes (46 in humans) discontinuous variation: variation that is “either/or” eg blood groups, ability to roll tongue or not DNA: t ...
Microarrays = Gene Chips
Microarrays = Gene Chips

... 6. Wash off any unstuck PCR products 7. Use a laser to detect the fluorescent dyes and create a visual image of the pattern of the dyes 8. If the PCR product has stuck on it will glow 9. The computer can then say which of the bacterial species the PCR products have stuck to and this indicates which ...
Gene mutations
Gene mutations

... During DNA replication, mistakes can be made when DNA polymerase adds complementary nucleotides. If this mutation or mistake happens very early on in a baby’s development, the mutation can affect the entire baby. The rest of the cells will have that same mutation. Remember, we all start off as one c ...
File
File

...  The making of proteins. RNA acts as a bridge between ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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