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BIO 304 Genetics
BIO 304 Genetics

... 9. snRNA_______ This class of RNA is involved in pre-mRNA splicing in eukaryotes. 10. primer______ A short nucleic acid fragment that is extended at its 3’ end in DNA synthesis. 11. P site_______ The region of a ribosomal large subunit to which peptidyl tRNA binds. aminoacyl tRNA 12. synthetase___ T ...
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... 6) ______ You wish to probe a human cDNA library to find out which insert has the β-globin gene. Which of the following would be a good choice as your probe (assume in all cases your probe is labeled)? A. B. C. D. E. ...
Unit 3
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... 2. Explain reasons why the cell cycle must be regulated in order for any human to be healthy during growth, development, and maintenance of the body. 3. Draw a diagram or flow chart of the order of the major processes and steps involved in the cell cycle (including mitosis). What are the major event ...
Nat 3 Cell Biology Homework
Nat 3 Cell Biology Homework

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Slide 1

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Nucleic Acids - Biology Innovation

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... fragments of DNA from another organism – enzyme is used to cut open plasmid – fragment then joins or splices into the plasmid = “Designer Genes”?  Average –  Genetic engineering –  Also allows scientists to give organisms genes from other species which selective breeding cannot  Crop plants –  ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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