GENE EXPRESSION - Doctor Jade Main
... • others remain quiescent • some function at all times • 30,000 are expressed in nearly all cell types • housekeeping genes – carry out basic metabolic processes • called constitutive • other genes are regulated – turned on or off as needed ...
... • others remain quiescent • some function at all times • 30,000 are expressed in nearly all cell types • housekeeping genes – carry out basic metabolic processes • called constitutive • other genes are regulated – turned on or off as needed ...
1 Biotechnology and Recombinant DNA
... Cannot digest (host) DNA with methylated cytosines Purified REs used in genetic engineering A specific RE always recognizes and cuts DNA at a very specific DNA nucleotide sequence. e.g. enzyme EcoRI - GAATTC ...
... Cannot digest (host) DNA with methylated cytosines Purified REs used in genetic engineering A specific RE always recognizes and cuts DNA at a very specific DNA nucleotide sequence. e.g. enzyme EcoRI - GAATTC ...
Answers chapter 9
... enzyme to make an incision in the newly synthesized strand (which it recognizes by virtue of it containing the unmethylated half of hemi-methylated GATC sites). The helicase UvrD then unwinds the DNA near the incision (and including the mismatch), and the unwound DNA is digested by an exonuclease. T ...
... enzyme to make an incision in the newly synthesized strand (which it recognizes by virtue of it containing the unmethylated half of hemi-methylated GATC sites). The helicase UvrD then unwinds the DNA near the incision (and including the mismatch), and the unwound DNA is digested by an exonuclease. T ...
DNA structure
... a. A cap is added to their 5’end. b. A poly-A tail is added to their 3’ end. c. Each usually specifies only a single protein. d. All of the above are true for eukaryotic mRNA, and false for prokaryotic mRNA. ...
... a. A cap is added to their 5’end. b. A poly-A tail is added to their 3’ end. c. Each usually specifies only a single protein. d. All of the above are true for eukaryotic mRNA, and false for prokaryotic mRNA. ...
Sequence Alignment Introduction
... obtain a “DNA barcode,” a short DNA sequence unique to a particular species, which is used to identify the species it belongs to. For animals and many other eukaryotes, different genes have been used for this purpose. One example is the mitochondrial cytochrome oxidase subunit I (COI) gene, which ...
... obtain a “DNA barcode,” a short DNA sequence unique to a particular species, which is used to identify the species it belongs to. For animals and many other eukaryotes, different genes have been used for this purpose. One example is the mitochondrial cytochrome oxidase subunit I (COI) gene, which ...
Slide 1
... they are commonly used to multiply or express particular genes. Plasmids used in genetic engineering are called vectors. Vectors are vehicles to transfer genes from one organism to another and typically contain a genetic marker conferring a phenotype. Most also contain a polylinker or multiple cloni ...
... they are commonly used to multiply or express particular genes. Plasmids used in genetic engineering are called vectors. Vectors are vehicles to transfer genes from one organism to another and typically contain a genetic marker conferring a phenotype. Most also contain a polylinker or multiple cloni ...
Science at the heart of medicine William R. Jacobs, Jr., Ph.D.
... In 1987, Dr. Jacobs set up his own lab at Einstein and began to isolate mycobacterial phages from the dirt in his backyard. His phage collection has grown over the years, thanks to high school students in his summer Phage Phinders program. Dr. Jacobs uses these phages to genetically manipulate mycob ...
... In 1987, Dr. Jacobs set up his own lab at Einstein and began to isolate mycobacterial phages from the dirt in his backyard. His phage collection has grown over the years, thanks to high school students in his summer Phage Phinders program. Dr. Jacobs uses these phages to genetically manipulate mycob ...
lect 8- Transformation
... • Some bacteria are naturally competent. • These bacteria have special protein spanning on their envelope • e.g Bacillus subtilis, Haemophilus influenzae • Regulate transformation competence by quorum sensing ...
... • Some bacteria are naturally competent. • These bacteria have special protein spanning on their envelope • e.g Bacillus subtilis, Haemophilus influenzae • Regulate transformation competence by quorum sensing ...
Unit D Review
... Gregor ________________ was the first geneticist information gained from the study of one type of organism can be _________________ to other organisms proves inherited diseases are usually caused by ___________ Traits can be determined by one ___________ or several __________. Traits can also ...
... Gregor ________________ was the first geneticist information gained from the study of one type of organism can be _________________ to other organisms proves inherited diseases are usually caused by ___________ Traits can be determined by one ___________ or several __________. Traits can also ...
AQA Biology Question number Answer Marks Guidance 1 a i (In all
... degenerate and get same/different amino acid in sequence 2 Accept does not code for amino acids 4 Accept exons not joined together/introns not removed ...
... degenerate and get same/different amino acid in sequence 2 Accept does not code for amino acids 4 Accept exons not joined together/introns not removed ...
Slides #5B (Green)
... health and may be inherited as autosomal dominant, autosomal recessive, or X-linked traits. Mutations that cause serious disability early in life are usually rare because of their adverse effect on life expectancy and reproduction. ...
... health and may be inherited as autosomal dominant, autosomal recessive, or X-linked traits. Mutations that cause serious disability early in life are usually rare because of their adverse effect on life expectancy and reproduction. ...
Slides - Department of Computer Science
... • Current methods can directly sequence only relatively short (<1000bp long) DNA fragments in a single reaction • Automated DNA-sequencing instruments (using gel-filled capillaries) can sequence up to 384 DNA samples in a single batch (run) in up to 24 runs a day: ~ 3,000,000 ...
... • Current methods can directly sequence only relatively short (<1000bp long) DNA fragments in a single reaction • Automated DNA-sequencing instruments (using gel-filled capillaries) can sequence up to 384 DNA samples in a single batch (run) in up to 24 runs a day: ~ 3,000,000 ...
Document
... Cytokinesis – when animal cells pinch in dividing the cytoplasm in the last stage of mitosis Meiosis : cell division to create gametes (sex cells: sperm & egg) -diploid (2n) -- haploid (1n) -1 cell divides to make 4 haploid cells *** Crossing over during Prophase I allows more genetic variation!!! ...
... Cytokinesis – when animal cells pinch in dividing the cytoplasm in the last stage of mitosis Meiosis : cell division to create gametes (sex cells: sperm & egg) -diploid (2n) -- haploid (1n) -1 cell divides to make 4 haploid cells *** Crossing over during Prophase I allows more genetic variation!!! ...
Word Work File L_2.tmp
... 5. The backbone of each single DNA chain is formed by alternating deoxyribose and phosphate groups joined by phosphodiester linkages. 6. Each phosphate group is linked to the 5’ carbon of one deoxyribose and to the 3’ carbon of the other deoxyribose. 7. Hydrogen bonds form between adenine and thymin ...
... 5. The backbone of each single DNA chain is formed by alternating deoxyribose and phosphate groups joined by phosphodiester linkages. 6. Each phosphate group is linked to the 5’ carbon of one deoxyribose and to the 3’ carbon of the other deoxyribose. 7. Hydrogen bonds form between adenine and thymin ...
Nucleic Acids - Somma Science
... DNA is also known as the hereditary material or genetic information. It is found in genes, and its sequence of bases makes up a code. Between "starts" and "stops," the code carries instructions for the correct sequence of amino acids in a protein (see Figure 1.3). DNA and RNA have different function ...
... DNA is also known as the hereditary material or genetic information. It is found in genes, and its sequence of bases makes up a code. Between "starts" and "stops," the code carries instructions for the correct sequence of amino acids in a protein (see Figure 1.3). DNA and RNA have different function ...
Overview of recombinant technology
... A DNA sequence can be changed by copying errors introduced by DNA polymerase during replication and by environmental agents such as chemical mutagens or radiation If uncorrected, such changes may interfere with the ability of the cell to function DNA damage can be repaired by several mechanisms All ...
... A DNA sequence can be changed by copying errors introduced by DNA polymerase during replication and by environmental agents such as chemical mutagens or radiation If uncorrected, such changes may interfere with the ability of the cell to function DNA damage can be repaired by several mechanisms All ...
The Body in Motion
... the amplified product is linked to a fluorescent reporter molecule, the fluorescence is measured at each cycle. This allows the amplification to be monitored to optimize the efficiency of amplification. ...
... the amplified product is linked to a fluorescent reporter molecule, the fluorescence is measured at each cycle. This allows the amplification to be monitored to optimize the efficiency of amplification. ...
Examination in Gene Technology, TFKE38 2011-10-18
... L21. The figure below (figure 2) shows the DNA sequence and protein sequence of L21. NOTE: This question provides a total of 20 points a) Construct primers to find the gene in a cDNA library (2p) b) In order to quickly clone your desired fragments without the use of restriction digest instead you us ...
... L21. The figure below (figure 2) shows the DNA sequence and protein sequence of L21. NOTE: This question provides a total of 20 points a) Construct primers to find the gene in a cDNA library (2p) b) In order to quickly clone your desired fragments without the use of restriction digest instead you us ...
Chapter 10 - Power Point Presentation
... Synthesis is performed by an enzyme (Surprise!) - RNA Polymerase Promoter Sequence = Special region of DNA at the beginning of gene Promoter sequence recognized by RNA polymerase RNA Polymerase binds to the promoter and begins making mRNA This process is directional, it only reads in one direction ( ...
... Synthesis is performed by an enzyme (Surprise!) - RNA Polymerase Promoter Sequence = Special region of DNA at the beginning of gene Promoter sequence recognized by RNA polymerase RNA Polymerase binds to the promoter and begins making mRNA This process is directional, it only reads in one direction ( ...
DNA - Biology
... fragments will move through the pores in the gel at different rates. Larger pieces of DNA will move slowly and shorter pieces will move faster and further. Generally, a control sample can also be added to the gel to compare sizes of test DNA fragments. The lengths of the VNTR regions can then be mea ...
... fragments will move through the pores in the gel at different rates. Larger pieces of DNA will move slowly and shorter pieces will move faster and further. Generally, a control sample can also be added to the gel to compare sizes of test DNA fragments. The lengths of the VNTR regions can then be mea ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.