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RNA & Protein Synthesis
RNA & Protein Synthesis

... ribosome to make a specific protein. ...
on-chip
on-chip

... genomes or within biologically important regions such as promoters. This method is used to map chromatin structure and DNA binding sites of transcription factors andpolymerases.  Methylated DNA regions are accurately mapped using a combination of affinity-based enrichment, such as Methylated DNA Im ...
Day 4. Genes and Genetic Level of Organization
Day 4. Genes and Genetic Level of Organization

... You also learned that traits can be inherited-like natural eye color and blood type, or acquired, like bigger muscles or body weight. Inherited traits are passed from generation to generation through DNA, which are located in the nucleus of a cell. So guess what, we have more to add to our levels of ...
No Slide Title - Cloudfront.net
No Slide Title - Cloudfront.net

... Oogenesis: oogonia proliferate in the fetus, enter meiosis before birth and will remain arrested in development for up to 50 years; number is limited; released “one at a time.” Spermatogenesis: sperm begin development in testes at puberty and in the seminiferous tubules ...
What is genomics
What is genomics

... cut the segments of each fragment out separately. Use the ruler to measure the length of each segment within a fragment and write this length in the segment. Be sure to include the tip in the measurement if the segment comes to a point. You should see that the length of each segment on the fragment ...
Concept Sheet - Fredericksburg City Public Schools
Concept Sheet - Fredericksburg City Public Schools

... October 1990, the Human Genome Project (HGP) began. Its goal was to map and sequence human DNA as well as study the ethical, legal and social issues that relate to a better understanding of human’s genetic makeup. Completed in 2003, two years early, the HGP mapped all of the 30,000 genes on the 23 p ...
Slide 1
Slide 1

... Coding region – contains nucleotide sequence that encodes a specific protein product (this region will be translated) In eukaryotes: introns and exons Non-coding regions – contains nucleotide sequence that will get transcribed BUT not translated *Un-translated regions (UTR’s) Promoter regions – sequ ...
Biotechnology ppt
Biotechnology ppt

... which desired traits of certain plants and animals are selected and passed on to their future generations.  Breed only those plants or animals with ...
Chapter 12 Notes - Great Neck Public Schools
Chapter 12 Notes - Great Neck Public Schools

... E. This genetically engineered, recombinant bacterium is then cloned to generate many copies of the gene, which can be used directly or translated into protein by the bacteria (human insulin) ...
Week 9 Pre-Lecture Slides
Week 9 Pre-Lecture Slides

Lab - TeacherWeb
Lab - TeacherWeb

...  Sort the DNA nucleotides into 4 separate piles according to their nitrogenous base and count them. Check the front of the envelope to be sure they are all there. Let your teacher know if you are missing any nucleotides. ...
Topic 3.5 Transcription (9-13)
Topic 3.5 Transcription (9-13)

Protein Synthesis & Mutation
Protein Synthesis & Mutation

... Proteins = courses of a meal • Recipes for all polypeptides are encoded by DNA • mRNA is a copy of that recipe (DNA sequence) • mRNA (recipes) travel to ribosomes for translation into polypeptides (proteins) ...
Name
Name

... 7. Transcription in prokaryotes and eukaryotes is similar in that: a. transcriptional machinery controls compaction and decompaction of chromatin b. the mRNA produced can undergo alternative splicing c. both prokaryotic and eukaryotic proteins have identical affinities for DNA d. both are contained ...
Location of Exons in DNA Sequences Using Digital Filters
Location of Exons in DNA Sequences Using Digital Filters

... across the two strands. A always pairs with T and G always pairs with C; in effect, the two strands are ...
Genomic Signal Processing - Electrical and Computer Engineering
Genomic Signal Processing - Electrical and Computer Engineering

... across the two strands. A always pairs with T and G always pairs with C; in effect, the two strands are ...
Mutations
Mutations

... – Change in second position often either does nothing or changes one amino acid for a similar one ...
posted
posted

... tRNAs must deliver amino acids corresponding to each codon The conformation (three-dimensional shape) of tRNA results from base pairing (hydrogen bonding) within the molecule. 3‘-end is the amino-acid attachment site—binds covalently. At the other end (middle of the tRNA sequence) is the Anticodon—s ...
MBLG1001 Lecture 9 The Flow of Genetic Information Replication
MBLG1001 Lecture 9 The Flow of Genetic Information Replication

... • At this rate it takes 40 min to copy the whole E. coli genome (4.6 million bases pairs) and another 20 min to separate the cellular components. • To double in less than 60 min means the cell must initiate the next round of replication before the previous one had finished. • To scale up this proces ...
Lecture
Lecture

... • Bacterial restriction enzymes cut DNA molecules at specific DNA sequences called restriction sites • A restriction enzyme usually makes many cuts, yielding restriction fragments • The most useful restriction enzymes cut DNA in a staggered way, producing fragments with “sticky ends.” Animation: Res ...
S2 Science - Kelso High School
S2 Science - Kelso High School

... A DNA database is a computer that stores information about people’s DNA b. A DNA profile contains detailed information about one person’s DNA 8. This will be your personal opinion based on the discussions you had in class. 9. Might be useful for:  Identifying and prosecuting criminals  Identifying ...
Slide 1
Slide 1

... 40 000 detectors in parallel, each detecting a specific DNA sequence. “Combinatorial Chemistry” ...
Cellular Reproduction
Cellular Reproduction

... dividing the cytoplasm between two cells ...
Reviews - Mi Portal
Reviews - Mi Portal

... Double-strand chromosome breaks can arise in a number of ways, by ionizing radiation, by spontaneous chromosome breaks during DNA replication, or by the programmed action of endonucleases, such as in meiosis. Broken chromosomes can be repaired either by one of several homologous recombination mechan ...
Genetics Option - Worked Examples
Genetics Option - Worked Examples

... DNA fingerprinting has been a crucially important breakthrough in crime analysis, enabling forensic scientists to identify a suspect from a tiny sample of blood, semen, saliva or other tissue. Before this, they could only identify blood groups and a few proteins, which could provide only a very roug ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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