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Genetics Study Guide Chapter 11, 13, 14
Genetics Study Guide Chapter 11, 13, 14

... Reading a DNA sequence, editing a DNA sequence, and reinserting DNA into living organisms are all techniques involved in what process? The use of nucleotides, gels, and fluorescent dyes are important in what technique? Suppose a restriction enzyme recognizes the eight base sequence TTGACTAA AACTGATT ...
Molecular Biology of the Cell
Molecular Biology of the Cell

Immobilization and stretching of DNA molecules in a
Immobilization and stretching of DNA molecules in a

... molecules for single-molecule DNA analysis applications. DNA-protein interactions drive the cellular machinery for maintaining and transcribing DNA. To study the motion and kinetics of proteins along a DNA strand at the single-molecule level, it is critical that the DNA molecules be stretched and im ...
CST Review PowerPoint
CST Review PowerPoint

... How many codons are located on the messenger RNA molecule in the diagram? ...
Protein Synthesis
Protein Synthesis

... • Clover-leaf shape • Single stranded molecule with attachment site at one end for an amino acid • Found out in the cytoplasm • Brings amino acid to ribosome ...
SMRT Sequencing of DNA and RNA Samples Extracted
SMRT Sequencing of DNA and RNA Samples Extracted

... Recent advances in next-generation sequencing have led to the increased use of formalin-fixed and paraffin-embedded (FFPE) tissues for medical samples in disease and scientific research. Single Molecule, Real-Time (SMRT) Sequencing offers a unique advantage in that it allows direct analysis of FFPE ...
Standard Operating Procedure for the Determination of Tissue
Standard Operating Procedure for the Determination of Tissue

슬라이드 1
슬라이드 1

... + ATR (ataxia telangiectasia and Rad3-related protein) is a serine/threonine-specific protein kinase that is involved in sensing DNA damage and activating the DNA damage checkpoint, leading to cell cycle arrest. - ATR is assisted by Ddc2 - Rad53 (CHK2 in humans) protein kinase ...
Protein Synthesis Notes File
Protein Synthesis Notes File

... I. Protein Synthesis A. ____________________- the process by which DNA is copied to RNA 1. The enzyme responsible for transcription is ____________________________ 2. RNA polymerase must bind with DNA at a _______________________ a) ___________________- series of repeating nucleotides up stream of t ...
Manipulating and Analyzing DNA
Manipulating and Analyzing DNA

... DNA and gel electrophoresis. You will use two different websites to understand both topics. By the end of today you should be able answer the flooring questions: What are restriction enzymes? How and why are they used in biotechnology? How do restriction enzymes play a role in recombinant DNA? How d ...
Genomic research concepts and application
Genomic research concepts and application

... DNA sequencing The method that underlies most sequencing approaches was originally  proposed in 1977 by Frederick Sanger, who is the only chemist to have  received two Nobel Prizes in Chemistry, the first as the sole recipient in  1958 for his work as the first to sequence a protein, the sequencing ...
See Fig. 13.1c
See Fig. 13.1c

... Virulence Plasmids - there are a number of ways that a plasmid can confer virulence in a bacterium. 1) The production of one or more toxins that can be directed toward the host or towards other bacteria (bacteriocins). 2) The ability to form a capsule. The recent anthrax scare is an example: Virulen ...
TrueAllele Report
TrueAllele Report

... definitive, or when there are multiple contributors to the evidence, uncertainty arises. This uncertainty is expressed in the resulting genotype, which may describe different genetic identity possibilities. Such genotype uncertainty may translate into reduced identification information when a compar ...
An Overview of Protein Synthesis
An Overview of Protein Synthesis

... 1) Anti-sense strand (Template strand) – the strand of DNA that is transcribed. Has the complementary genetic code of the mRNA. The tRNA will have the same base sequence as the anti-sense strand (except U instead of T) 2) Sense strand (coding strand) – the strand of DNA that is not transcribed. It h ...
Bacterial Variation
Bacterial Variation

03 Nucleic Acids
03 Nucleic Acids

... DNA is also known as the hereditary material or genetic information. It is found in genes, and its sequence of bases makes up a code. Between "starts" and "stops," the code carries instructions for the correct sequence of amino acids in a protein (see Figure 1.3). DNA and RNA have different function ...
Introduction to Molecular Cell Biology (not tought by SK in 2010)
Introduction to Molecular Cell Biology (not tought by SK in 2010)

... Mitochondria have their own DNA, which replicates independent of the nuclear DNA 2. Genetic code of the mitochondria is different from the main code of the cell 3. Mitochondria have their own ribosomes on which some of the mitochondrial proteins are produced. Others are imported from the outside 4. ...
DNA Technologies
DNA Technologies

cell - Wando High School
cell - Wando High School

... they may be closely packed together or no growth factor is present. • Cancer begins as a single cell • This cell is normally found and destroyed by the body’s immune system. If not, this cell could divide into a mass of identical daughter cancer cells that: – Impair the function of one or more organ ...
ppt document, 1.2 MB
ppt document, 1.2 MB

Slide 1
Slide 1

... Proteins are made using amino acids joined together by peptide bonds 20 different amino acids The code consists of 4 letters:  A, U, C, and G (RNA bases) The genetic code is read 3 letters at a time  mRNA “Codon” = 3 bases (AUG)  tRNA “Anti-codon” = 3 complimentary bases (UAC)  64 possible 3-bas ...
Mutation - TeacherWeb
Mutation - TeacherWeb

...  Full set in somatic cells NOT sex cells DNA codes for protein. Influences appearance  Involved in bodily processes  Involved in bodily repair ...
lecture 12, part 2, dna technology, 050509c
lecture 12, part 2, dna technology, 050509c

... DNA technology continues to be used in identifying small fragments of human remains from the terror attacks of September 11, 2001. DNA has been used to exonerate prisoners who were innocent of the ...
Exploring Genes
Exploring Genes

...  can grow large quantities of M13  can sequence foreign DNA cloned into M13 with dideoxy method ...
RNA & Protein Synthesis
RNA & Protein Synthesis

... ribosome to make a specific protein. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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