Genetics Option - Worked Examples
... DNA fingerprinting has been a crucially important breakthrough in crime analysis, enabling forensic scientists to identify a suspect from a tiny sample of blood, semen, saliva or other tissue. Before this, they could only identify blood groups and a few proteins, which could provide only a very roug ...
... DNA fingerprinting has been a crucially important breakthrough in crime analysis, enabling forensic scientists to identify a suspect from a tiny sample of blood, semen, saliva or other tissue. Before this, they could only identify blood groups and a few proteins, which could provide only a very roug ...
Isolation and Purification of Total Genomic DNA from Gram
... DNA for analysis by gel electrophoresis, for PCR, and for a couple restriction digests. • PURITY: What level of contaminants (protein, RNA, etc.) can be tolerated? The purification method must eliminate any contaminants that would interfere with subsequent steps. This depends, of course, on what you ...
... DNA for analysis by gel electrophoresis, for PCR, and for a couple restriction digests. • PURITY: What level of contaminants (protein, RNA, etc.) can be tolerated? The purification method must eliminate any contaminants that would interfere with subsequent steps. This depends, of course, on what you ...
Cell cycle and Reproduction - River Dell Regional School District
... 1. It temporarily holds two daughter DNA double helices together after DNA replication 2. It is the attachment site for microtubules that move the chromosomes during cell division ...
... 1. It temporarily holds two daughter DNA double helices together after DNA replication 2. It is the attachment site for microtubules that move the chromosomes during cell division ...
Genetic Modification in Food Production
... 3. Advantages and disadvantages 4. Examples ( golden rice, sweet corn and long- lasting tomatoes) ...
... 3. Advantages and disadvantages 4. Examples ( golden rice, sweet corn and long- lasting tomatoes) ...
Beyond Mendel: Molecular genetics, cell division, and sex
... − the shape is described by its secondary structure, tertiary structure, and quaternary structure, which we will not cover in detail − the shape has a huge effect on the chemical properties of the protein − the shape is largely determined by the sequence of amino acids: the protein’s primary structu ...
... − the shape is described by its secondary structure, tertiary structure, and quaternary structure, which we will not cover in detail − the shape has a huge effect on the chemical properties of the protein − the shape is largely determined by the sequence of amino acids: the protein’s primary structu ...
Cell and DNA summary
... A DNA database is a computer that stores information about people’s DNA b. A DNA profile contains detailed information about one person’s DNA 8. This will be your personal opinion based on the discussions you had in class. 9. Might be useful for: Identifying and prosecuting criminals Identifying ...
... A DNA database is a computer that stores information about people’s DNA b. A DNA profile contains detailed information about one person’s DNA 8. This will be your personal opinion based on the discussions you had in class. 9. Might be useful for: Identifying and prosecuting criminals Identifying ...
Using a Single-Nucleotide Polymorphism to Predict
... Verify GMO-negative result is not due to PCR reaction not working ...
... Verify GMO-negative result is not due to PCR reaction not working ...
Analyzing Data
... • The DNA molecule is hydrophilic (water-soluble) but cell membranes are made of a very hydrophobic lipid bilayer. Two means of artificial transformation commonly used in labs: electroporation and chemical transformation. • During electroporation, short bursts of current are passed through a solutio ...
... • The DNA molecule is hydrophilic (water-soluble) but cell membranes are made of a very hydrophobic lipid bilayer. Two means of artificial transformation commonly used in labs: electroporation and chemical transformation. • During electroporation, short bursts of current are passed through a solutio ...
Genetics 2
... and subjected to a restriction enzyme that is associated with the production of restriction fragments that an investigator wishes to examine. One only requires a method of observing the size of the DNA fragments that result from the use of the restriction enzymes. This is the purpose of the techniqu ...
... and subjected to a restriction enzyme that is associated with the production of restriction fragments that an investigator wishes to examine. One only requires a method of observing the size of the DNA fragments that result from the use of the restriction enzymes. This is the purpose of the techniqu ...
Lecture 10: Meiosis Products of meiosis in animals vs. plants, fungi
... - Crossing over of homologous chromosomes occurs during prophase I Mechanism by which recombination creates new combination of alleles - Cutting, pasting, and crossing over of DNA backbones yields new combination of alleles Mechanism by which recombination creates copy number variation (CNV) - Durin ...
... - Crossing over of homologous chromosomes occurs during prophase I Mechanism by which recombination creates new combination of alleles - Cutting, pasting, and crossing over of DNA backbones yields new combination of alleles Mechanism by which recombination creates copy number variation (CNV) - Durin ...
Lecture 2 - Organic Origins Debate
... Rapid encephalisation of the brain: 1 to 3 lb. brain in only 2 m years Machiavellian intelligence Climate change Ballistic hunting Language and group size Sexual selection ...
... Rapid encephalisation of the brain: 1 to 3 lb. brain in only 2 m years Machiavellian intelligence Climate change Ballistic hunting Language and group size Sexual selection ...
DNA - Madison Public Schools
... The sex of a DNA contributor can be determined by looking at the amelogenin gene. The gene is 6 bases shorter on the X chromosome than it is on the Y chromosome. Y-STR’s – STR’s found on the Y chromosome; useful when there is a mix of DNA from more than one ...
... The sex of a DNA contributor can be determined by looking at the amelogenin gene. The gene is 6 bases shorter on the X chromosome than it is on the Y chromosome. Y-STR’s – STR’s found on the Y chromosome; useful when there is a mix of DNA from more than one ...
PS Webquest
... 5. Where does the energy to form the peptide bond between two amino acids come from? ...
... 5. Where does the energy to form the peptide bond between two amino acids come from? ...
aa + aa + aa + aa aa – aa – aa – aa
... 3. The long chans of amino acids (known as_________________________) coil up to create a ______________ (working) protein. The protein will not work unless it is folded up. 4. Most amino acids look exactly the same except for the _____ group or ________________ group. 5. There are _______ different ...
... 3. The long chans of amino acids (known as_________________________) coil up to create a ______________ (working) protein. The protein will not work unless it is folded up. 4. Most amino acids look exactly the same except for the _____ group or ________________ group. 5. There are _______ different ...
Exam 3 Spring 2007 and key
... D. a large number of alleles of each gene E. none of the above 2. Which technique would be best for screening a large number of genes at the same time for detection of mutant genes? A. southern blotting D. PCR B. northern blotting E. DNA microarray C. western blotting 3. Enzymes that recognize a spe ...
... D. a large number of alleles of each gene E. none of the above 2. Which technique would be best for screening a large number of genes at the same time for detection of mutant genes? A. southern blotting D. PCR B. northern blotting E. DNA microarray C. western blotting 3. Enzymes that recognize a spe ...
Restriction Fragment Length Polymorphisms
... different DNA sequences and therefore produce different fragments when a particular restriction enzyme is used to cut an equivalent section of DNA. •The fragments produced are called RFLPs, ...
... different DNA sequences and therefore produce different fragments when a particular restriction enzyme is used to cut an equivalent section of DNA. •The fragments produced are called RFLPs, ...
DNA Detectives What is Your DNA Alias? The central dogma of
... We use four letters to code all the information contained in DNA: A, T, C and G. The letters are used in groups of three. A group is called a codon. DNA contains the information that is needed by your body to make proteins. The different proteins have specific functions, such as making our hearts, h ...
... We use four letters to code all the information contained in DNA: A, T, C and G. The letters are used in groups of three. A group is called a codon. DNA contains the information that is needed by your body to make proteins. The different proteins have specific functions, such as making our hearts, h ...
Techniques of gene therapy
... Once the gene that causes a disease has been identified, the corresponding normal gene must be isolated, unless it is already available because it has been studied for some other purpose. Using an abnormal gene to find its normal counterpart is usually done by exploiting the extensive similarity bet ...
... Once the gene that causes a disease has been identified, the corresponding normal gene must be isolated, unless it is already available because it has been studied for some other purpose. Using an abnormal gene to find its normal counterpart is usually done by exploiting the extensive similarity bet ...
PDF (black and white)
... cross-pollinated true-breeding plants to carry out his experiment. What were Mendel's two experiments? In his first experiment, Mendel studied 7 characteristics. He performed crosses ...
... cross-pollinated true-breeding plants to carry out his experiment. What were Mendel's two experiments? In his first experiment, Mendel studied 7 characteristics. He performed crosses ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.