LECTURE 10 Viruses I. Properties of viruses. 1. They are obligate
... 4. Prions - are small, altered proteins (a gene product). These infectious proteins cause normally folded proteins in the brain to become misfolded. a. Mad cow disease (bovine spongiform encephalopathy) is the result of a prion. ...
... 4. Prions - are small, altered proteins (a gene product). These infectious proteins cause normally folded proteins in the brain to become misfolded. a. Mad cow disease (bovine spongiform encephalopathy) is the result of a prion. ...
DNA: The Molecule of Inheritance
... Inject mice with live S bacteriamice die, live S cells in blood Inject mice with dead S bacteriamice live, no live S cells in blood Inject mice with live R bacteria + dead S bacteriamice die, live S cells in ...
... Inject mice with live S bacteriamice die, live S cells in blood Inject mice with dead S bacteriamice live, no live S cells in blood Inject mice with live R bacteria + dead S bacteriamice die, live S cells in ...
Plasmids are fragments of double-stranded DNA that can replicate
... Short segment of DNA which contains several restriction sites allowing for the easy insertion of DNA. In expression plasmids, the MCS is often downstream from a promoter. Gene, promoter or other DNA fragment cloned into the MCS for further study. Drives transcription of the target gene. Vital compon ...
... Short segment of DNA which contains several restriction sites allowing for the easy insertion of DNA. In expression plasmids, the MCS is often downstream from a promoter. Gene, promoter or other DNA fragment cloned into the MCS for further study. Drives transcription of the target gene. Vital compon ...
Document
... 3. A parent has blood type O. What blood types are possible for his/her children? Are any blood types not possible for his/her children? Why? ...
... 3. A parent has blood type O. What blood types are possible for his/her children? Are any blood types not possible for his/her children? Why? ...
Exam3-1406_Spring'06.doc
... C) addition of exons to the mRNA. D) deletion of exons from the mRNA. E) combination of two different genes together. ...
... C) addition of exons to the mRNA. D) deletion of exons from the mRNA. E) combination of two different genes together. ...
Nucleic Acid Structure:
... ! There are two enzymes in E.coli that help aid in the transcription process: 1. Core Enzyme: helps by catalytic RNA synthesis and contains four types of polypeptide chain. 2. Sigma Factor Enzyme: has no catalytic activity but helps the core enzyme recognize the start genes. Once RNA synthesis begin ...
... ! There are two enzymes in E.coli that help aid in the transcription process: 1. Core Enzyme: helps by catalytic RNA synthesis and contains four types of polypeptide chain. 2. Sigma Factor Enzyme: has no catalytic activity but helps the core enzyme recognize the start genes. Once RNA synthesis begin ...
Extensions for LIC
... NOTE: It is essential to amplify a genomic gene fragment that includes a unique restriction site in order to linearize the construct prior to transfection. A 1kb fragment is sufficient for targeting, but it may be necessary to amplify a longer fragment so that it contains a unique site. The site sho ...
... NOTE: It is essential to amplify a genomic gene fragment that includes a unique restriction site in order to linearize the construct prior to transfection. A 1kb fragment is sufficient for targeting, but it may be necessary to amplify a longer fragment so that it contains a unique site. The site sho ...
Table of Contents - Baton Rouge Community College
... Learning Outcomes: Upon successful completion of this course, the students will be able to: 1. Demonstrate knowledge of genetic principles and problem solving skills. 2. Relate an organism’s genotype to its phenotype. 3. Explain basic techniques used for genetic manipulation and procedures used to i ...
... Learning Outcomes: Upon successful completion of this course, the students will be able to: 1. Demonstrate knowledge of genetic principles and problem solving skills. 2. Relate an organism’s genotype to its phenotype. 3. Explain basic techniques used for genetic manipulation and procedures used to i ...
Central dogma of molecular biology
... The central dogma of molecular biology was first enunciated by Francis Crick in 1958 and restated in a Nature paper published in 1970.The central dogma deals with the detailed residue-by-residue transfer of sequential information. It states that information cannot be transferred back from protein to ...
... The central dogma of molecular biology was first enunciated by Francis Crick in 1958 and restated in a Nature paper published in 1970.The central dogma deals with the detailed residue-by-residue transfer of sequential information. It states that information cannot be transferred back from protein to ...
7.5 Eukaryotic Genome Regulation
... 3 One strand of each short doublestranded RNA is degraded; the other strand (miRNA) then associates with a complex of proteins. ...
... 3 One strand of each short doublestranded RNA is degraded; the other strand (miRNA) then associates with a complex of proteins. ...
Archaea are prokaryotic
... It turns out that they have their own versions of these enzymes that are specially equipped to work at high temperatures. In 1976, Chien, Edgar, and Trela isolated a DNA polymerase from T. aquaticus, and found that it had a temperature optimum of 80 C. (Compare this to the 37 C optimum of most nucle ...
... It turns out that they have their own versions of these enzymes that are specially equipped to work at high temperatures. In 1976, Chien, Edgar, and Trela isolated a DNA polymerase from T. aquaticus, and found that it had a temperature optimum of 80 C. (Compare this to the 37 C optimum of most nucle ...
Document
... i. loss of variation per generation is 1-1/(2N). ii. Waiting time for random alleles to find a common ancestor is 2N. Factors that influences Ne: i. Variance in offspring. WF: 1. If variance is higher, then effective population size is smaller. ...
... i. loss of variation per generation is 1-1/(2N). ii. Waiting time for random alleles to find a common ancestor is 2N. Factors that influences Ne: i. Variance in offspring. WF: 1. If variance is higher, then effective population size is smaller. ...
Intimate Strangers
... are missing many of the others. In general, viruses are entirely composed of a single strand of genetic information encased within a protein capsule. Viruses lack most of the internal structure and machinery which characterize “life”. In order for a virus to replicate it must infect a suitable host ...
... are missing many of the others. In general, viruses are entirely composed of a single strand of genetic information encased within a protein capsule. Viruses lack most of the internal structure and machinery which characterize “life”. In order for a virus to replicate it must infect a suitable host ...
You Light Up My Life
... 2) RNA is translated to form polypeptide chains, which fold to form proteins ...
... 2) RNA is translated to form polypeptide chains, which fold to form proteins ...
RNA and Transcription Worksheet File
... In what part of the cell does the process above (question 14) occur? ...
... In what part of the cell does the process above (question 14) occur? ...
et al
... Gel contains Ethidium Bromide, which binds to DNA and fluoresces when hit with UV light (WEAR GLOVES!!!) ...
... Gel contains Ethidium Bromide, which binds to DNA and fluoresces when hit with UV light (WEAR GLOVES!!!) ...
What is another name for a polypeptide?
... the right to complete your Chromosomal Mutations “Mutations Concept Map” ...
... the right to complete your Chromosomal Mutations “Mutations Concept Map” ...
Summary sheet - ThinkChemistry
... the cell. To see the structures within the cell, it is usually necessary to use a _____________. ...
... the cell. To see the structures within the cell, it is usually necessary to use a _____________. ...
Protein Synthesis Worksheet
... 5. (mRNA / rRNA) is used to carry the genetic code from DNA to the ribosomes. 6. (DNA / RNA) uses uracil instead of thymine. 7. (DNA / RNA) can leave the nucleus. 8. one piece of code or codon is made of (one / three) nucleotide monomers. 9. The DNA is copied into mRNA by (dna polymerase / rna polym ...
... 5. (mRNA / rRNA) is used to carry the genetic code from DNA to the ribosomes. 6. (DNA / RNA) uses uracil instead of thymine. 7. (DNA / RNA) can leave the nucleus. 8. one piece of code or codon is made of (one / three) nucleotide monomers. 9. The DNA is copied into mRNA by (dna polymerase / rna polym ...
DNA as the Genetic Material
... a change in genotype and phenotype due tot he assimilation by a cell of external DNA 4. By exposing the harmless bacteria to different chemicals from the heat-killed pathogenic variety, Griffith noticed that only DNA caused transformation of the bacteria. C. In 1952, Alfred Hershey and Martha Chase ...
... a change in genotype and phenotype due tot he assimilation by a cell of external DNA 4. By exposing the harmless bacteria to different chemicals from the heat-killed pathogenic variety, Griffith noticed that only DNA caused transformation of the bacteria. C. In 1952, Alfred Hershey and Martha Chase ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.