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Genetics SHOW
Genetics SHOW

... 1) DNA is found inside the Nucleus of our cells. 2) DNA makes a single stranded copy of itself. This is called RNA. 3) RNA is similar to DNA, containing 4 base pairs, with one different letter (U instead of T) 4) This RNA moves out of the nucleus (called messenger RNA or mRNA) 5) mRNA travels to the ...
Name: Date: Genetic Engineering Notes Selective Breeding: People
Name: Date: Genetic Engineering Notes Selective Breeding: People

...  Some bacteria are able to naturally able to pick up DNA and sometimes humans control it  Scientists create a piece of DNA called a plasmid  These plasmids need to have a origin for replication and two ends that a specific restriction enzyme will cut  Wherever it cuts the bacterial DNA is where ...
DNA RNA protein DNA REPLICATION
DNA RNA protein DNA REPLICATION

... The genetic material is stored in the form of DNA in most organisms. In humans, the nucleus of each cell contains 3 × 109 base pairs of DNA distributed over 23 pairs of chromosomes, and each cell has two copies of the genetic material. This is known collectively as the human genome. The human genome ...
Chapter 10 Manipulating Genes
Chapter 10 Manipulating Genes

... Specialized Plasmid Vectors Are Used to Clone DNA Investigators interested in cloning, however find it easier to manipulated, copy, and purify their recombinant DNA when it is maintained as an independent molecule, separate from the bacterial chromosome. To maintain foreign DNA in a bacterial cell, ...
Searching for the “Secret of Life”
Searching for the “Secret of Life”

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Brooker Chapter 10
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... Three types of DNA sequences are required for chromosome replication and segregation ...
AP Test Genetics Review
AP Test Genetics Review

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17.1 – Isolating the Genetic Material
17.1 – Isolating the Genetic Material

... of the replicating fork during DNA replication → DNA polymerase – during DNA replication, an enzyme that slips into the space between two strands, uses the parent strands as a template, and adds nucleotides to make complimentary strands → replication fork – during DNA replication, point at which the ...
Discovering the material for heredity: DNA
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Genetic Engineering
Genetic Engineering

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Human Genetics Lec 4
Human Genetics Lec 4

... chromosomes are arranged in pairs; one member of the pair is inherited from the father, the other from the mother. Each species has a characteristic number of chromosomes. In the human, 46 single or 23 pairs of chromosomes are present. Of the 23 pairs of human chromosomes, there are 22 pairs called ...
A Novel Interacting Protein With The DNA Mismatch Repair Gene
A Novel Interacting Protein With The DNA Mismatch Repair Gene

... (HNPCC), the most common known genetic cancer susceptibility syndrome. We are investigating protein interactions between the DNA MMR protein PMS2 and Clone PMS2-Interactor 1 to explain the dominant negative (DN) mechanism of DNA MMR failure. The N-terminus of PMS2 is known to react with other protei ...
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... Information from a specific section of DNA is first transcribed to produce a specific molecule of RNA RNA attaches to a ribosome where the information is translated into a corresponding sequence of amino acids ...
Genetics - Mobile County Public Schools
Genetics - Mobile County Public Schools

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Exam 2 Student Key

... Please write ONLY TWO! You can use the same answer for different descriptions.) 2 pts each a. Protein that can interact with DNA in a helicase, DNA pol I,III, ligase, RNA pol… non-specific manner (bind any sequence). b. Enzyme that can break phosphodiester bonds DNA pol I, III, topoisomerase c. Spec ...
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... beams, at the tips of which one or more double helix structures running in parallel are located. These have been modified such that each end carries one base pair. Two of these microscopic beams are connected with a flexible polymer. On the other side, the beams are coupled to microscopic spheres wh ...
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Gregor Mendel - father of Genetics and 18th century Austrian monk
Gregor Mendel - father of Genetics and 18th century Austrian monk

... Nondisjunction - The failure of a chromosome pair to separate during meiosis. - Body (somatic) cells may have more or less than normal amount of chromosomes, usually lethal. Karyotype - Shows the size, shape and number of chromosomes in an organism. Down Syndrome: An extra chromosome (trisomy) on th ...
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DNAandGeneticsEducDept

... couple who are planning to have a child but who suspect that there is a greater than normal risk of the child being affected by a genetic disorder ...
Overview of the Recombinant DNA technology- the plasmid vector pUC19
Overview of the Recombinant DNA technology- the plasmid vector pUC19

... together. As a result, the foreign gene (CIH-1) from pBK-CMV is ligated into the MCS of pCU19. However, the desired outcome from the cloning experiment is that one vector molecule to be joined to one of the genomic DNA fragments in order to circularize and form a new recombinant molecule. The last s ...
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Summary notes for ch1-6
Summary notes for ch1-6

... (ii) Activation energy, active sites and induced fit - Activation energy is the energy needed to contort the molecule into a highly unstable state (Transition state) so that chemical bonds can be broken and a chemical reaction can take place. Out with cells, this energy is provided in the form of he ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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