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Protein Synthesis
Protein Synthesis

... There are 20 essential amino acids, however they can be combined in any order, just like the four nucleotides. This permits the production of the many different proteins which let organisms grow and function. ...
AP Biology Ch. 20 - apbiologyclass / FrontPage
AP Biology Ch. 20 - apbiologyclass / FrontPage

... Problems with expressing eukaryotic genes in bacterial host cells • Scientists use an expression vector, a cloning vector that contains a highly active prokaryotic promoter • This allows the bacteria to recognize the promoter and proceed to express the foreign gene. • This allows synthesis of many ...
- ISpatula
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... Lacl gene is constitutive, its gene product, repressor, is active. Unlike inducible lacZ, lacY and lacA genes, whose expression is co-ordinately regulated ...
1 BIOS 1300 SI SI WORKSHEET 8 (Chapter 3 Cont.) SI Leader
1 BIOS 1300 SI SI WORKSHEET 8 (Chapter 3 Cont.) SI Leader

... -Alternate RNA splicing allows 1 pre mRNA to code for multiple proteins III. Translation: 1. Initiation: mRNA, a tRNA corresponding to the __________ codon, and 2 ribosomal 
 subunits unite to form a translation initiation complex with the help of 
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Introduction
Introduction

... 1. Hereditary variations: Among the sexually reproducing organisms no two individuals have the same heredity. The differences in hereditary constitutions of the individuals of a species are known as hereditary or genetical variations. 2. Environmental variations: The variations which are not inheri ...
Bits and pieces come to life
Bits and pieces come to life

... uncracked code as well, but over the past century, scientists have slowly learned how to read the genetic code that every living cell contains. They have figured out which genes determine which characteristics of cells and organisms, and how changes to genes can alter these characteristics. Now rese ...
Bits and pieces come to life
Bits and pieces come to life

... uncracked code as well, but over the past century, scientists have slowly learned how to read the genetic code that every living cell contains. They have figured out which genes determine which characteristics of cells and organisms, and how changes to genes can alter these characteristics. Now rese ...
DNA and the Genome
DNA and the Genome

Photosynthesis - Mrs. Brenner's Biology
Photosynthesis - Mrs. Brenner's Biology

...  Members of a bacterial colony on a petri dish are clones because they all came from division of the same cell.  Identical twins are clones • A single embryo separates to become two. ...
Density Gradient Centrifugation
Density Gradient Centrifugation

... the actual path taken by bio-molecules is much longer than the length of the gel allowing for better separation. (Think about the resolution obtained on a chromatographic column) As shown before, one of the clever methods to sequence DNA in seventies was to subject single stranded DNA to specific en ...
May 27, 2017 The Difference Makers
May 27, 2017 The Difference Makers

... 4. Would evolution still happen if there were no transposons, retrotransposons or retroviruses messing with the genome? [Yes, but it would probably be slower, relying on ordinary mutations that arise during DNA replication when cells divide or genetic recombination of chromosomes in a new generati ...
Cross-Curricular Discussion
Cross-Curricular Discussion

... 4. Would evolution still happen if there were no transposons, retrotransposons or retroviruses messing with the genome? [Yes, but it would probably be slower, relying on ordinary mutations that arise during DNA replication when cells divide or genetic recombination of chromosomes in a new generati ...
Chapter 20 Notes AP Biology I. Chapter 20.1: DNA - Pomp
Chapter 20 Notes AP Biology I. Chapter 20.1: DNA - Pomp

... iii. Natural  function  is  to  protect  the  bacterial  cell  against   intruding  DNA  from  other  organisms   1. Work  by  cutting  up  foreign  DNA   iv. Hundreds  of  restriction  enzymes  that  are  very  specific   1. Restriction  sit ...
Chem 464 Biochemistry
Chem 464 Biochemistry

... Hypochromism refers to the fact that DNA has a lower absorbance at 260 nm that you would calculate based on the sum of the absorbancies of the monomers. This occurs because the bases stacked in the core of the helix have electronic interactions with the bases above and below them that change their l ...
Gene Cloning
Gene Cloning

... A protein is encoded by a coding region which is the part of the gene between the translation initiation codon (normally ATG)and the translation termination codon (TAA, TGA or TAG) It is important that you appreciate the difference between a gene and a coding ...
Evolution of prokaryotic genomes
Evolution of prokaryotic genomes

... (d) Site-specific recombination can generate genetic variation upon use of secondary crossing-over sites Site-specific DNA inversion systems have been thoroughly studied both in vivo and in vitro with bacteria, bacteriophages and plasmids (Glasgow et al., 1989). An example is the periodic inversion ...
Exam 2 practice questions organized by lecture topic
Exam 2 practice questions organized by lecture topic

... B. vitamin A during first three months of embryonic/fetal development C. alcohol D. german measles E. all of the above 48. In 1927, U.T. Austin Scientist H. J. Muller irradiated male drosophilia and showed that radiation induced lethal genes in the X chromosome. The drosophilia model used by Muller ...
DNA Tech
DNA Tech

... What effect does selective breeding have on a population’s variation? It will usually decrease variation because we eliminate some variations in favor of an extreme ...
Name: :______ Genetic Mutations—Online Model Go to: http
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Exam #3 Review
Exam #3 Review

... other molecules can bind to them and affect their ability to bind to the DNA: -Some repressors can’t bind to the operator site until another molecule (called a corepressor) binds to them and allows them to bind to the operator and block transcription (e.g. the trp operon). - Some repressors bind to ...
BIOFINALRVW
BIOFINALRVW

... 1. Who were the scientists responsible for discovering that DNA was the heredity material? ...
Genetics and Protein Synthesis
Genetics and Protein Synthesis

... Allele - an alternate form of a gene. Usually there are two alleles for every gene, sometimes as many a three or four. Homozygous - when the two alleles are the same. Heterozygous - when the two alleles are different, in such cases the dominant allele is expressed. Dominant - a term applied to the t ...
Chapter 2 - CSUB Home Page
Chapter 2 - CSUB Home Page

... Mendel’s hypothesis (to explain his results) 1. Alternative versions of genes (alleles) account for variation in inherited characters 2. For each character, an organism inherits two alleles, one from each parent 3. If two alleles differ, then one (the dominant allele) is fully expressed in the organ ...
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses

... microscopic scissors that cut at specific sites consistently. 3. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. ...
11/11/15 - cloudfront.net
11/11/15 - cloudfront.net

... Keep your answers covered If you need to make up a quiz due to an absence… come see me Tues or Thurs during PLC Flip it over when you are finished and hang on to it ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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