State of the Art Manufacturing of Protein Hydrolysates
... Sometimes the filter aid is mixed with the protein digest and recycles through the filter press until a nice coat is formed before the clear filtrate is collected and this process is often referred to as “body-feed coating”. Charcoal powder is commonly used to decolorize and to remove haze-forming c ...
... Sometimes the filter aid is mixed with the protein digest and recycles through the filter press until a nice coat is formed before the clear filtrate is collected and this process is often referred to as “body-feed coating”. Charcoal powder is commonly used to decolorize and to remove haze-forming c ...
1st Sem (unit I)
... More than three hundred different amino acids have been described in nature, only twenty (20) are commonly found in mammalian proteins. Each amino acid (except Proline) has a carboxyl group (-COOH), an amino group (-NH2 ) and a distinctive side chain (-R group) bonded to -carbon atom (fig 1.1) At ...
... More than three hundred different amino acids have been described in nature, only twenty (20) are commonly found in mammalian proteins. Each amino acid (except Proline) has a carboxyl group (-COOH), an amino group (-NH2 ) and a distinctive side chain (-R group) bonded to -carbon atom (fig 1.1) At ...
Towards the construction of Escherichia coli cell
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
A siderophore biosynthesis gene cluster from the fish
... Abbreviations: A domain, adenylation domain; ArCP, aryl-carrier protein; DAHP, 3-deoxy-D-arabino-heptulosonate-7-phosphate; HPI, highpathogenicity island; NRPS, non-ribosomal peptide synthetase; SSH, suppression subtractive hybridization. The GenBank/EMBL/DDBJ accession number for the sequences repo ...
... Abbreviations: A domain, adenylation domain; ArCP, aryl-carrier protein; DAHP, 3-deoxy-D-arabino-heptulosonate-7-phosphate; HPI, highpathogenicity island; NRPS, non-ribosomal peptide synthetase; SSH, suppression subtractive hybridization. The GenBank/EMBL/DDBJ accession number for the sequences repo ...
Food derived from glufosinate ammonium
... Tu494. It codes for the enzyme phosphinothricin acetyl transferase (PAT), which modifies and inactivates the herbicide glufosinate ammonium (Strauch et al, 1988). The pat gene is often used as a selectable marker to distinguish genetically modified plant cells from unmodified cells. In this applicat ...
... Tu494. It codes for the enzyme phosphinothricin acetyl transferase (PAT), which modifies and inactivates the herbicide glufosinate ammonium (Strauch et al, 1988). The pat gene is often used as a selectable marker to distinguish genetically modified plant cells from unmodified cells. In this applicat ...
Summary of risk management plan and specific licence conditions
... vigour is greatest in the first generation and declines in subsequent generations. Plant breeders have often used male sterile plants to accomplish hybrid seed production. The male sterile line of InVigor® canola contains the barnase gene from the soil bacterium Bacillus amyloliquefaciens. The barna ...
... vigour is greatest in the first generation and declines in subsequent generations. Plant breeders have often used male sterile plants to accomplish hybrid seed production. The male sterile line of InVigor® canola contains the barnase gene from the soil bacterium Bacillus amyloliquefaciens. The barna ...
WebMOTIFS: Web-based integrated motif discovery
... Program Description and Organization WebMOTIFS is designed to automate the identification of regulatory sequence motifs using multiple motif discovery algorithms. Users may provide gene names (RefSeq or yeast ORF names) or probe identifiers from one of several microarray platforms for S. cerevisiae ...
... Program Description and Organization WebMOTIFS is designed to automate the identification of regulatory sequence motifs using multiple motif discovery algorithms. Users may provide gene names (RefSeq or yeast ORF names) or probe identifiers from one of several microarray platforms for S. cerevisiae ...
Dreze et al, Methods Enzymol 2010
... because they contain a cryptic AD (cognate autoactivators), and (iii) nontranscription factor proteins that contain one or more cryptic ADs that are only functional as truncated fragments and not when expressed in the context of full-length proteins (de novo autoactivators). Both genuine transcripti ...
... because they contain a cryptic AD (cognate autoactivators), and (iii) nontranscription factor proteins that contain one or more cryptic ADs that are only functional as truncated fragments and not when expressed in the context of full-length proteins (de novo autoactivators). Both genuine transcripti ...
Protein Structure - Macmillan Learning
... removed by centrifugation, to yield a “crude extract.” The crude extract is then fractionated to separate the proteins and isolate the one that is of particular interest, using a variety of techniques. One of the most powerful methods is column chromatography, in which the protein mixture is applied ...
... removed by centrifugation, to yield a “crude extract.” The crude extract is then fractionated to separate the proteins and isolate the one that is of particular interest, using a variety of techniques. One of the most powerful methods is column chromatography, in which the protein mixture is applied ...
PDF - Oxford Academic
... DNA sequences in both genes corresponding to restriction enzyme cleavage sites were verified by cleavage with the appropriate restriction enzyme with one exception. In the Lbc gene a DNA sequence corresponding to a Clal cleavage site was determined at nucleotide positions 1024-1029. This sequence ha ...
... DNA sequences in both genes corresponding to restriction enzyme cleavage sites were verified by cleavage with the appropriate restriction enzyme with one exception. In the Lbc gene a DNA sequence corresponding to a Clal cleavage site was determined at nucleotide positions 1024-1029. This sequence ha ...
Codon optimization of Col H gene encoding Clostridium
... site obstructs translation initiation. On the other hand, GC-poor mRNAs cannot fold strongly and ...
... site obstructs translation initiation. On the other hand, GC-poor mRNAs cannot fold strongly and ...
Coarse-Grained Modeling of ProteinDynamics
... 2010; Best and Hummer 2008). Despite their simplicity, in many cases they correctly capture unfolding pathways, FEL and mechanical stability of proteins. For example, a complete description of mechanical unfolding pathways of single and multidomain Ubiquitin at the level of secondary structure was o ...
... 2010; Best and Hummer 2008). Despite their simplicity, in many cases they correctly capture unfolding pathways, FEL and mechanical stability of proteins. For example, a complete description of mechanical unfolding pathways of single and multidomain Ubiquitin at the level of secondary structure was o ...
- Wiley Online Library
... which is a subset of the entire sequence database. The third algorithm is a profile–profile alignment algorithm BASIC ~Rychlewski et al., 1998! developed in our group and used previously to assign folds to proteins from several genomes ~Rychlewski et al., 1998, 1999; Pawlowski et al., 1999!, in a fo ...
... which is a subset of the entire sequence database. The third algorithm is a profile–profile alignment algorithm BASIC ~Rychlewski et al., 1998! developed in our group and used previously to assign folds to proteins from several genomes ~Rychlewski et al., 1998, 1999; Pawlowski et al., 1999!, in a fo ...
Synthetic Peptides as Antigens for Antibody Production
... usually bacteria, and the resultant purified protein used as an immunogen. Glutathione-S-transferase fusion proteins, for example, have been extensively used as immunogens. Alternatively, small synthetic peptides can be synthesized that contain amino acid sequences derived from the cDNA acid or gene ...
... usually bacteria, and the resultant purified protein used as an immunogen. Glutathione-S-transferase fusion proteins, for example, have been extensively used as immunogens. Alternatively, small synthetic peptides can be synthesized that contain amino acid sequences derived from the cDNA acid or gene ...
A study of archaeal enzymes involved in polar lipid
... study, we focused on the steps in archaeal phospholipid synthetic pathways that generate polar lipids such as archaetidylserine, archaetidylglycerol, and archaetidylinositol. Only archaetidylserine synthase (ASS), from Methanothermobacter thermautotrophicus, has been experimentally identified. Other ...
... study, we focused on the steps in archaeal phospholipid synthetic pathways that generate polar lipids such as archaetidylserine, archaetidylglycerol, and archaetidylinositol. Only archaetidylserine synthase (ASS), from Methanothermobacter thermautotrophicus, has been experimentally identified. Other ...
Protein Structure
... Some proteins are fibrous and have great strength. They make up the structural parts of cells. Some proteins are globular and serve as transport, regulatory and enzyme ...
... Some proteins are fibrous and have great strength. They make up the structural parts of cells. Some proteins are globular and serve as transport, regulatory and enzyme ...
Protein translation in Plasmodium parasites
... Peptide exit tunnel: the site, formed by proteins and rRNA, where the nascent polypeptide chain leaves the ribosome. P-granules: translationally silent storage particles containing high levels of non-translated mRNA. Related to P-bodies, but differ in their inability to degrade mRNA. Plastid: endosy ...
... Peptide exit tunnel: the site, formed by proteins and rRNA, where the nascent polypeptide chain leaves the ribosome. P-granules: translationally silent storage particles containing high levels of non-translated mRNA. Related to P-bodies, but differ in their inability to degrade mRNA. Plastid: endosy ...
Cloning and sequencing of a gene encoding acidophilic amylase
... Agnano 101 (Buonocore et at., 1976) and A-2 (Kanno, 1986), and from Bacillus sp. 11-1s (Uchino, 1982). The pH optima of the three enzymes ranged from 2-0 to 3.5, temperature optima between 70 and 75 "C and apparent molecular masses from 54 to 68 kDa. The molecular mass of the B. acidocaldarius ATCC ...
... Agnano 101 (Buonocore et at., 1976) and A-2 (Kanno, 1986), and from Bacillus sp. 11-1s (Uchino, 1982). The pH optima of the three enzymes ranged from 2-0 to 3.5, temperature optima between 70 and 75 "C and apparent molecular masses from 54 to 68 kDa. The molecular mass of the B. acidocaldarius ATCC ...
Regulation of Arabidopsis 14-3
... Since 14-3-3 proteins regulate several aspects N metabolism in plants, we were interested to determine whether the 14-3-3s themselves might be subject to regulation by N metabolites. We therefore examined the expression of all members of the 14-3-3 gene family in Arabidopsis seedlings grown with dif ...
... Since 14-3-3 proteins regulate several aspects N metabolism in plants, we were interested to determine whether the 14-3-3s themselves might be subject to regulation by N metabolites. We therefore examined the expression of all members of the 14-3-3 gene family in Arabidopsis seedlings grown with dif ...
¹⁵N Heteronuclear Single Quantum Coherence
... group binds with alternate orientations of 180° along the α,γ-meso axis (see Figure 2). Heme re-orientation will then progress until the equilibrium ratio of 9:1 is reached. NMR spectroscopy has been effective at monitoring this distribution of conformational isomers. Paramagnetism causes resonances ...
... group binds with alternate orientations of 180° along the α,γ-meso axis (see Figure 2). Heme re-orientation will then progress until the equilibrium ratio of 9:1 is reached. NMR spectroscopy has been effective at monitoring this distribution of conformational isomers. Paramagnetism causes resonances ...
all plant protein - frequently asked questions
... NUTRILITE™ All Plant Protein contains a unique tri-blend of soy, wheat, and pea protein to give you the right combination of amino acids to keep you feeling healthy and energetic, without animal products or dairy side effects. 4. How does this product work? NUTRILITE™ All Plant Protein provides high ...
... NUTRILITE™ All Plant Protein contains a unique tri-blend of soy, wheat, and pea protein to give you the right combination of amino acids to keep you feeling healthy and energetic, without animal products or dairy side effects. 4. How does this product work? NUTRILITE™ All Plant Protein provides high ...
recombinant protein characterization
... and effective monitoring techniques is being driven by the need to better understand the biological production processes, as well as a need for improved control of feeding of the organisms producing the recombinant protein and other process parameters. Production processes must also be robust, stand ...
... and effective monitoring techniques is being driven by the need to better understand the biological production processes, as well as a need for improved control of feeding of the organisms producing the recombinant protein and other process parameters. Production processes must also be robust, stand ...
Delivering of Proteins to the Plant Vacuole—An Update
... PSV [4,17]. Contrarily to the initial observations, direct ER-to-Vacuole transport is not limited to storage proteins being already described for tonoplast aquaporins and processing enzymes [18,19]. Transport to the lytic vacuole occurs after recognition of the sorting signal by the receptor BP-80 ( ...
... PSV [4,17]. Contrarily to the initial observations, direct ER-to-Vacuole transport is not limited to storage proteins being already described for tonoplast aquaporins and processing enzymes [18,19]. Transport to the lytic vacuole occurs after recognition of the sorting signal by the receptor BP-80 ( ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.