Comparison of sequence-based and structure
... the families have low average sequence identities, three families have high average %I but have poor correlation coefficient. These families are either from multi-domain or small protein classes. Further investigations on these three families revealed possible reasons for the poor correlation between ...
... the families have low average sequence identities, three families have high average %I but have poor correlation coefficient. These families are either from multi-domain or small protein classes. Further investigations on these three families revealed possible reasons for the poor correlation between ...
The significance of biochemical and molecular sample integrity in
... state and the rapidity of death, may play major roles in determining the postmortem condition of the sample [6–9]. It would therefore be of importance to determine the condition and quality of postmortem tissue, for example, through the quantification of a biological marker in such sample. In a prev ...
... state and the rapidity of death, may play major roles in determining the postmortem condition of the sample [6–9]. It would therefore be of importance to determine the condition and quality of postmortem tissue, for example, through the quantification of a biological marker in such sample. In a prev ...
Dietary Guidelines should reflect new understandings about adult
... This concept of substrate adequacy is evaluated by shortterm nitrogen retention. Titration of amino acids into the diet from protein-free to surfeit intakes produces an almost linear response in nitrogen balance from negative to positive. Nitrogen balance (i.e. intake = excretion) is assumed to refl ...
... This concept of substrate adequacy is evaluated by shortterm nitrogen retention. Titration of amino acids into the diet from protein-free to surfeit intakes produces an almost linear response in nitrogen balance from negative to positive. Nitrogen balance (i.e. intake = excretion) is assumed to refl ...
NTPase/helicase of Flaviviridae: inhibitors and inhibition of the
... et al., 1996) have shown that removal of the NH2-terminal 148 amino acids enhanced the NTPase activity of JEV NTPase/helicase. On the other hand, a comparative study with recombinant full-length NS3 and its isolated NH2- and COOH-terminal domains, performed by Gallinari et al. (1998), did not reveal ...
... et al., 1996) have shown that removal of the NH2-terminal 148 amino acids enhanced the NTPase activity of JEV NTPase/helicase. On the other hand, a comparative study with recombinant full-length NS3 and its isolated NH2- and COOH-terminal domains, performed by Gallinari et al. (1998), did not reveal ...
Hydrogen exchange mass spectrometry for the analysis of protein
... simply because they are technically simpler to perform. Far fewer experiments are of the pulse labeling variety. Generally in pulse labeling experiments (Fig. 1, top left), a population of protein molecules is either induced to undergo some kind of conformational change by addition of a perturbing a ...
... simply because they are technically simpler to perform. Far fewer experiments are of the pulse labeling variety. Generally in pulse labeling experiments (Fig. 1, top left), a population of protein molecules is either induced to undergo some kind of conformational change by addition of a perturbing a ...
Comparison of Rumen Amino Acid Protection Technologies
... Glutamic acid Glutamine Glycine Proline Serine Tyrosine ...
... Glutamic acid Glutamine Glycine Proline Serine Tyrosine ...
Crossing borders to bind proteins—a new concept in protein
... molecule consists of a small organic molecule or peptide that is linked with or without an interconnecting spacer to a 42-residue polypeptide selected from a set of only sixteen sequences (Fig. 1) [12]. The small molecule must bind to the protein but only modest affinity is required. Conjugation to ...
... molecule consists of a small organic molecule or peptide that is linked with or without an interconnecting spacer to a 42-residue polypeptide selected from a set of only sixteen sequences (Fig. 1) [12]. The small molecule must bind to the protein but only modest affinity is required. Conjugation to ...
Reverse Transcription PCR (RT-PCR): The Molecular
... latency that can last for several years. The proviral DNA is replicated along with the cellular DNA and can be inherited through many generations. The HIV proviral DNA contains the major genes common to all non-transducing retroviruses. These genes are gag, pol and env (see Figure 2). HIV also conta ...
... latency that can last for several years. The proviral DNA is replicated along with the cellular DNA and can be inherited through many generations. The HIV proviral DNA contains the major genes common to all non-transducing retroviruses. These genes are gag, pol and env (see Figure 2). HIV also conta ...
Journal of Agricultural and Food Chemistry
... mass between 45 and 50 kDa. Electrophoretic patterns of albumin-2 fractions showed that the main protein subunits were concentrated in (kDa): 34.2 for amaranth and 36.4 for soybean. The electrophoretic separation of these plants showed similarity. Variations in protein separation of amaranth and soy ...
... mass between 45 and 50 kDa. Electrophoretic patterns of albumin-2 fractions showed that the main protein subunits were concentrated in (kDa): 34.2 for amaranth and 36.4 for soybean. The electrophoretic separation of these plants showed similarity. Variations in protein separation of amaranth and soy ...
The sequence of human serum albumin cDNA and its expression in
... the i n i t i a t i o n codon ATG followed by the 12 nucleotides coding f o r the f i r s t four amino acids of mature HSA as determined by DNA sequence analysis of clone F-47. In a process referred to as "primer repair", the gene containing Pstl fragment of F-47 was denatured, annealed with excess ...
... the i n i t i a t i o n codon ATG followed by the 12 nucleotides coding f o r the f i r s t four amino acids of mature HSA as determined by DNA sequence analysis of clone F-47. In a process referred to as "primer repair", the gene containing Pstl fragment of F-47 was denatured, annealed with excess ...
1 Are the Eyes Homologous?
... expressed in those locations. It seems reasonable to conclude that it must be near the top of the developmental hierarchy for eye development. A mutation in a similar protein in mammals (Pax6, the eyeless homologue, based on sequence and motif similarities) results in abnormal formations of the eye. ...
... expressed in those locations. It seems reasonable to conclude that it must be near the top of the developmental hierarchy for eye development. A mutation in a similar protein in mammals (Pax6, the eyeless homologue, based on sequence and motif similarities) results in abnormal formations of the eye. ...
Nutritional Genomics
... GO terms - Tube development, Immune response, Cytokine binding InterPro motifs - Several terms associated with immunoglobulin motifs KEGG pathway - cytokine-cytokine receptor interaction Remarkable down-regulation of gene expression due to maternal methionine supplementation ...
... GO terms - Tube development, Immune response, Cytokine binding InterPro motifs - Several terms associated with immunoglobulin motifs KEGG pathway - cytokine-cytokine receptor interaction Remarkable down-regulation of gene expression due to maternal methionine supplementation ...
Protein Engineering in the Development of
... and motifs used in the responsive materials. We also highlight protein domains with unrealized potential for hydrogel materials applications. One of the most widely exploited protein domains is the α-helical coiled-coil or leucine zipper domain. Coiled coils have been extensively studied (23–29) and ...
... and motifs used in the responsive materials. We also highlight protein domains with unrealized potential for hydrogel materials applications. One of the most widely exploited protein domains is the α-helical coiled-coil or leucine zipper domain. Coiled coils have been extensively studied (23–29) and ...
Full Text - the American Society of Animal Science
... The experiment was conducted in accordance with national regulations for human care and use of animals in research. Licenses, procedures, and holding facilities were approved by the French Veterinary Services (certificate of authorization of experiment on living animals no. 35-22 delivered by the Fr ...
... The experiment was conducted in accordance with national regulations for human care and use of animals in research. Licenses, procedures, and holding facilities were approved by the French Veterinary Services (certificate of authorization of experiment on living animals no. 35-22 delivered by the Fr ...
Inhibition of Wound-Induced Accumulation of
... expression can be prevented by SA or aspirin (Doherty et al., 1988; Peña-Cortés et al., 1993; Doares et al., 1995). Previous results have shown that SA or aspirin inhibit the conversion of 13-HPLA to 12-oxo-PDA, thereby inhibiting the signal pathway by blocking the synthesis of JA (PeñaCortés et ...
... expression can be prevented by SA or aspirin (Doherty et al., 1988; Peña-Cortés et al., 1993; Doares et al., 1995). Previous results have shown that SA or aspirin inhibit the conversion of 13-HPLA to 12-oxo-PDA, thereby inhibiting the signal pathway by blocking the synthesis of JA (PeñaCortés et ...
CitA (citrate) and DcuS (C 4 -dicarboxylate) sensor kinases in
... in addition to the citrate that was demonstrated earlier as a substrate. The lack of growth on ...
... in addition to the citrate that was demonstrated earlier as a substrate. The lack of growth on ...
TRANSLATION OF mRNA - E-Learning/An
... to understand the nature of the gene by studying morphological traits. However, Beadle and Tatum realized that morphological traits are likely to be based on systems of biochemical reactions so complex as to make analysis exceedingly difficult. Therefore, they turned their genetic studies to the ana ...
... to understand the nature of the gene by studying morphological traits. However, Beadle and Tatum realized that morphological traits are likely to be based on systems of biochemical reactions so complex as to make analysis exceedingly difficult. Therefore, they turned their genetic studies to the ana ...
Mutational effects on protein structure and function Jonas Carlsson Link¨
... In this thesis several important proteins are investigated from a structural perspective. Some of the proteins are disease related while other have important but not completely characterised functions. The techniques used are general as demonstrated by applications on metabolic proteins (CYP21, CYP1 ...
... In this thesis several important proteins are investigated from a structural perspective. Some of the proteins are disease related while other have important but not completely characterised functions. The techniques used are general as demonstrated by applications on metabolic proteins (CYP21, CYP1 ...
Thiele et al.: `Genome-scale reconstruction of E. coli`s transcriptional
... [4Fe-4S] cluster [25,68,69]. Function of TrmD was determined [70]. The reaction schema for radical S-Adenosyl-L-methionine (amet) enzymes was described in [71,72]. Thermogota protein is monomer [70]. No further information found, so the protein was assumed to be a monomer. Pierrel et al showed that ...
... [4Fe-4S] cluster [25,68,69]. Function of TrmD was determined [70]. The reaction schema for radical S-Adenosyl-L-methionine (amet) enzymes was described in [71,72]. Thermogota protein is monomer [70]. No further information found, so the protein was assumed to be a monomer. Pierrel et al showed that ...
Isolation and characterization of (S)
... plants are derived from the amino acids tyrosine, tryptophan and phenylalanine. They are often basic and contain nitrogen in a heterocyclic ring. The classification of alkaloids is based on their carbon-nitrogen skeletons; common alkaloid ring structures include the pyridines, pyrroles, indoles, pyr ...
... plants are derived from the amino acids tyrosine, tryptophan and phenylalanine. They are often basic and contain nitrogen in a heterocyclic ring. The classification of alkaloids is based on their carbon-nitrogen skeletons; common alkaloid ring structures include the pyridines, pyrroles, indoles, pyr ...
Biotechnology Explorer - Bio-Rad
... In this lab, your students will perform a procedure known as genetic transformation. Genetic transformation occurs when a cell takes up (takes inside) and expresses a new piece of genetic material—DNA. This new genetic information often provides the organism with a new trait which is identifiable af ...
... In this lab, your students will perform a procedure known as genetic transformation. Genetic transformation occurs when a cell takes up (takes inside) and expresses a new piece of genetic material—DNA. This new genetic information often provides the organism with a new trait which is identifiable af ...
Function of Conserved Tryptophans in the Aspergillus niger
... residues of G1 are important for fully functional starch binding. Large deletions of SBD residues result in alteration of the folding and stability properties of the protein and eliminate ligand binding. Recently, the solution structure of the G1 SBD has been solved by nuclear magnetic resonance (NM ...
... residues of G1 are important for fully functional starch binding. Large deletions of SBD residues result in alteration of the folding and stability properties of the protein and eliminate ligand binding. Recently, the solution structure of the G1 SBD has been solved by nuclear magnetic resonance (NM ...
Proteomics Principles and Techniques Prof. Sanjeeva Srivastava
... with the release of water molecules. This linear sequence of amino acids constitutes the primary structure. Let us now discuss about secondary structure, which refers to locally folded regions. The folding of polypeptide or protein chain in to regular structures like alpha helices, beta sheets turns ...
... with the release of water molecules. This linear sequence of amino acids constitutes the primary structure. Let us now discuss about secondary structure, which refers to locally folded regions. The folding of polypeptide or protein chain in to regular structures like alpha helices, beta sheets turns ...
State of the Art Manufacturing of Protein Hydrolysates
... Sometimes the filter aid is mixed with the protein digest and recycles through the filter press until a nice coat is formed before the clear filtrate is collected and this process is often referred to as “body-feed coating”. Charcoal powder is commonly used to decolorize and to remove haze-forming c ...
... Sometimes the filter aid is mixed with the protein digest and recycles through the filter press until a nice coat is formed before the clear filtrate is collected and this process is often referred to as “body-feed coating”. Charcoal powder is commonly used to decolorize and to remove haze-forming c ...
Supporting information S1.
... phospotransferase (SmR) as a positive selection marker and multiple cloning sites. This plasmid only replicates in bacterial hosts supplying in trans the π protein encoded by the pir gene [1]. We generated pKNG derivative plasmids containing: a) an E. coli DNA chromosomal fragment, b) an optimized p ...
... phospotransferase (SmR) as a positive selection marker and multiple cloning sites. This plasmid only replicates in bacterial hosts supplying in trans the π protein encoded by the pir gene [1]. We generated pKNG derivative plasmids containing: a) an E. coli DNA chromosomal fragment, b) an optimized p ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.