R26 :: CAG GCaMP6f - The Jackson Laboratory
... international biomedical research community - adds hundreds of new strains annually. The JAX Mouse Repository is supported by NIH, The Howard Hughes Medical Institute and several private charitable foundations. ...
... international biomedical research community - adds hundreds of new strains annually. The JAX Mouse Repository is supported by NIH, The Howard Hughes Medical Institute and several private charitable foundations. ...
Mitochondrial protein acetylation regulates metabolism
... Post-translational acetylation of proteins is the covalent addition of an acetyl group to the ε-amino group of lysine residues that occurs on a wide array of proteins. This simple modification neutralizes the positive charge of the lysine residue, potentially altering its propensity to interact with ...
... Post-translational acetylation of proteins is the covalent addition of an acetyl group to the ε-amino group of lysine residues that occurs on a wide array of proteins. This simple modification neutralizes the positive charge of the lysine residue, potentially altering its propensity to interact with ...
chemical modification of carboxylic groups
... (1967), and then developed and enlarged by Weber & Osborn (1969) and Dunker & Rueckert (1969). Since that time, that simple technique has emerged as the most popular, easiest and cheapest method for determining the Mr values of protein subunits (for a complete review of the method see See & Jackowsk ...
... (1967), and then developed and enlarged by Weber & Osborn (1969) and Dunker & Rueckert (1969). Since that time, that simple technique has emerged as the most popular, easiest and cheapest method for determining the Mr values of protein subunits (for a complete review of the method see See & Jackowsk ...
Interacting specificity of a histidine kinase and its cognate response
... interaction. We first determined whether the yeast twohybrid system could be applied to detect specific interactions between the histidine kinase and its cognate response regulator. To employ the yeast two-hybrid assay, the genes encoding the PrrA response regulator and the PrrB histidine kinase wer ...
... interaction. We first determined whether the yeast twohybrid system could be applied to detect specific interactions between the histidine kinase and its cognate response regulator. To employ the yeast two-hybrid assay, the genes encoding the PrrA response regulator and the PrrB histidine kinase wer ...
Directed mutagenesis of the Trypanosoma cruzi trans
... The sequences of several genes encoding T. cruzi TS and TS-like proteins have been reported (Pereira et al., 1991; Pollevick et al., 1991; Uemura et al., 1992), and the predicted amino acid sequences of the subclass of enzymatically active gene products indicate that the parasite proteins have struc ...
... The sequences of several genes encoding T. cruzi TS and TS-like proteins have been reported (Pereira et al., 1991; Pollevick et al., 1991; Uemura et al., 1992), and the predicted amino acid sequences of the subclass of enzymatically active gene products indicate that the parasite proteins have struc ...
Table S2
... Table S2, continued Pds1: Inhibits the onset of anaphase by binding and sequestering the Esp1 protease that cleaves the cohesin complexes that hold sister chromatids together. Binding of Pds1 to Esp1 was reported to depend in Cdc28 phosphorylation[53] Sic1: Inhibitor of Clb-Cdc28. Phosphorylation o ...
... Table S2, continued Pds1: Inhibits the onset of anaphase by binding and sequestering the Esp1 protease that cleaves the cohesin complexes that hold sister chromatids together. Binding of Pds1 to Esp1 was reported to depend in Cdc28 phosphorylation[53] Sic1: Inhibitor of Clb-Cdc28. Phosphorylation o ...
Myrick LK, Nakamoto-Kinoshita M, Lindor NM, Warren ST , et al. Fragile X syndrome due to a missense mutation. European Journal of Human Genetics (2014) 22, 1185-1189.
... This gene harbors a CGG-repeat in its 50 untranslated region. In normal individuals, repeat length is polymorphic, with 29–30 repeats being the most common allele.4 In patients with FXS, there is a large expansion of the repeat sequence to over 200 repeats, referred to as the full mutation. Once the ...
... This gene harbors a CGG-repeat in its 50 untranslated region. In normal individuals, repeat length is polymorphic, with 29–30 repeats being the most common allele.4 In patients with FXS, there is a large expansion of the repeat sequence to over 200 repeats, referred to as the full mutation. Once the ...
ABSTRACT Title of Document:
... Table of Contents...........................................................................................................v List of Tables .............................................................................................................. vii List of Figures ............................ ...
... Table of Contents...........................................................................................................v List of Tables .............................................................................................................. vii List of Figures ............................ ...
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional
... delaying gastric emptying. Moreover, CCK is involved in the regulation of food intake and satiation. Secretion of CCK by the hindgut is controversial, and its biological activity remains to be elucidated. The present paper addresses the regional distribution of intestinal CCK in the white sea bream, ...
... delaying gastric emptying. Moreover, CCK is involved in the regulation of food intake and satiation. Secretion of CCK by the hindgut is controversial, and its biological activity remains to be elucidated. The present paper addresses the regional distribution of intestinal CCK in the white sea bream, ...
The Expanded Genetic Code Measurement Kit
... Expanding the Genetic Code Non-canonical Amino Acid tRNA Charged tRNA ...
... Expanding the Genetic Code Non-canonical Amino Acid tRNA Charged tRNA ...
Why nature chose phosphate to modify proteins
... The advantageous chemical properties of the phosphate ester linkage were exploited early in evolution to generate the phosphate diester linkages that join neighbouring bases in RNA and DNA (Westheimer 1987 Science 235, 1173 – 1178). Following the fixation of the genetic code, another use for phospha ...
... The advantageous chemical properties of the phosphate ester linkage were exploited early in evolution to generate the phosphate diester linkages that join neighbouring bases in RNA and DNA (Westheimer 1987 Science 235, 1173 – 1178). Following the fixation of the genetic code, another use for phospha ...
Comparative proteomic network signatures in seminal plasma of
... antioxidant defence mechanism(s) to fight off the noxious effects of ROS. However, when their rate of generation exceeds the cell’s antioxidant defence, it leads to oxidative stress. ROS are highly reactive, non-specific and autocatalytic which qualify them to be good signalling molecules. Physiolog ...
... antioxidant defence mechanism(s) to fight off the noxious effects of ROS. However, when their rate of generation exceeds the cell’s antioxidant defence, it leads to oxidative stress. ROS are highly reactive, non-specific and autocatalytic which qualify them to be good signalling molecules. Physiolog ...
Antifolding activity of hsp60 couples protein import into the
... Experiments were carried out to demonstrate a physical interaction between the pb2-DHFR fusion proteins and hsp60-14mer. The topology of mature-sized b2(331)DHFR arrested in contact sites in the presence of MTX was analyzed as a representative example (Figure 4). Upon removal of the MTX-stabilized D ...
... Experiments were carried out to demonstrate a physical interaction between the pb2-DHFR fusion proteins and hsp60-14mer. The topology of mature-sized b2(331)DHFR arrested in contact sites in the presence of MTX was analyzed as a representative example (Figure 4). Upon removal of the MTX-stabilized D ...
In vitro gastrointestinal digestion study of a novel bio-tofu
... these findings mentioned above, the novel food structures with improved properties such as controlled energy intake, good satiety and digestibility may develop rapidly in the future. Thus, enzymatic modification of proteins by MTGase could lead to firmer matrices that are digested to a lower extent ...
... these findings mentioned above, the novel food structures with improved properties such as controlled energy intake, good satiety and digestibility may develop rapidly in the future. Thus, enzymatic modification of proteins by MTGase could lead to firmer matrices that are digested to a lower extent ...
Variation in Glutenin Protein Subunits of Wheat
... Three further points may be noted concerning the different patterns in group 3 (Fig. 3). First, the single band of pattern h (see Fig. 3) appears on gels as an unusually broad band. However, when gels are run for longer periods (greater than 5 h) this broad band is resolved into two separate bands: ...
... Three further points may be noted concerning the different patterns in group 3 (Fig. 3). First, the single band of pattern h (see Fig. 3) appears on gels as an unusually broad band. However, when gels are run for longer periods (greater than 5 h) this broad band is resolved into two separate bands: ...
Bioinformatics
... Smith-Waterman dynamic programming algorithm 50X slower than BLAST or FASTA – faster hardware has made this problem feasible ...
... Smith-Waterman dynamic programming algorithm 50X slower than BLAST or FASTA – faster hardware has made this problem feasible ...
Ribosomes of Mouse Liver following
... cause a marked inhibition of hepatic protein synthesis (18). Although a number of disturbances in the protein synthetic apparatus have been found following DMNA treatment, the primary critical lesion has not been identified (19). Adminis tration of DMNA results in a rapid disorganization of the endo ...
... cause a marked inhibition of hepatic protein synthesis (18). Although a number of disturbances in the protein synthetic apparatus have been found following DMNA treatment, the primary critical lesion has not been identified (19). Adminis tration of DMNA results in a rapid disorganization of the endo ...
Synthetic human prion protein octapeptide repeat binds to the
... (residues 125–228) and an N-terminal disordered tail [7]. The fragment between residues 53 and 85 consists of repeats of the sequence GGGWGQPH and can be defined as an ‘‘octapeptide repeat (OPR) region.’’ This region plays an important role in the physiological function of prion proteins. In a model ...
... (residues 125–228) and an N-terminal disordered tail [7]. The fragment between residues 53 and 85 consists of repeats of the sequence GGGWGQPH and can be defined as an ‘‘octapeptide repeat (OPR) region.’’ This region plays an important role in the physiological function of prion proteins. In a model ...
Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine
... Riboswitches are regulatory elements modulating gene expression in response to specific metabolite binding. It has been recently reported that riboswitch agonists may exhibit antimicrobial properties by binding to the riboswitch domain. Guanine riboswitches are involved in the regulation of transpor ...
... Riboswitches are regulatory elements modulating gene expression in response to specific metabolite binding. It has been recently reported that riboswitch agonists may exhibit antimicrobial properties by binding to the riboswitch domain. Guanine riboswitches are involved in the regulation of transpor ...
Acyl Carrier Protein (ACP) lmport into Chloroplasts Does not
... 1980). lsolation and analysis of cDNAs generated from poly A’ selected RNA for a spinach ACP indicates that the genes for ACP are nuclear-encoded (Scherer and Knauf, 1987). Similar results have been presented for Brassica ACP (Safford et al., 1988). Nuclear-encoded chloroplast proteins are generally ...
... 1980). lsolation and analysis of cDNAs generated from poly A’ selected RNA for a spinach ACP indicates that the genes for ACP are nuclear-encoded (Scherer and Knauf, 1987). Similar results have been presented for Brassica ACP (Safford et al., 1988). Nuclear-encoded chloroplast proteins are generally ...
Distinct Classes of Mitotic Cyclins Are Differentially
... between Cyc4Gm and CycSGm. However, the identities between these two groups are only 23 to 29%. The amino acids conserved commonly in A- and B-type cyclins are highly conserved in all soybean cyclin sequences (81 to 94%). The amino acids conserved distinctively in A-type cyclins are conserved 63 to ...
... between Cyc4Gm and CycSGm. However, the identities between these two groups are only 23 to 29%. The amino acids conserved commonly in A- and B-type cyclins are highly conserved in all soybean cyclin sequences (81 to 94%). The amino acids conserved distinctively in A-type cyclins are conserved 63 to ...
The endogenous molecular clock orchestrates the temporal
... uptake [6,7]. It has been widely reported that skeletal muscle has regulatory mechanisms that modulate substrate utilization and storage in response to varying metabolic demands and environmental conditions (for example, nutrient status) [3,8-12]. For instance, skeletal muscle rapidly modulates rate ...
... uptake [6,7]. It has been widely reported that skeletal muscle has regulatory mechanisms that modulate substrate utilization and storage in response to varying metabolic demands and environmental conditions (for example, nutrient status) [3,8-12]. For instance, skeletal muscle rapidly modulates rate ...
RESEARCH ARTICLES Characterization of the Long
... depending on the difference in physicochemical properties and location in the protein structure; in order to evaluate these effects we used the insect LW portion of our Bayesian tree in TreeSAAP v3.0 (Woolley et al. 2003) to test for evidence of selection among 31 amino acid properties in the three ...
... depending on the difference in physicochemical properties and location in the protein structure; in order to evaluate these effects we used the insect LW portion of our Bayesian tree in TreeSAAP v3.0 (Woolley et al. 2003) to test for evidence of selection among 31 amino acid properties in the three ...
Chapter 2 - University of the Free State
... More disordered water molecules •∆G = ∆H - T∆S •∆S is the change in entropy (order) •Change from order to disorder gives a positive ∆S, and ∴ a -∆G •Processes where ∆G < 0 (i.e., negative) occur spontaneously •At higher temperature, the disorder in the free water molecules increase ∴transfer of a wa ...
... More disordered water molecules •∆G = ∆H - T∆S •∆S is the change in entropy (order) •Change from order to disorder gives a positive ∆S, and ∴ a -∆G •Processes where ∆G < 0 (i.e., negative) occur spontaneously •At higher temperature, the disorder in the free water molecules increase ∴transfer of a wa ...
Lecture 1 - "Hudel" Luecke
... is shown bound to an all-DNA substrate inhibitor (in blue), with the 5' end of the substrate (and the 3' end of the ribozyme) on the left of the image. The catalytic core of the ribozyme is shown in red, stem II in purple, and the substrate binding arms of the ribozyme (stems I and III) in green. ...
... is shown bound to an all-DNA substrate inhibitor (in blue), with the 5' end of the substrate (and the 3' end of the ribozyme) on the left of the image. The catalytic core of the ribozyme is shown in red, stem II in purple, and the substrate binding arms of the ribozyme (stems I and III) in green. ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.