A genetically encoded fluorescent tRNA is active in live
... need for ex vivo technologies. We show here that the fusion of a large RNA aptamer with one of the largest tRNAs, despite each having a well-defined tertiary structure, enables the tRNA to perform live-cell protein synthesis in Escherichia coli. This tRNA–aptamer fusion can be switched on with quant ...
... need for ex vivo technologies. We show here that the fusion of a large RNA aptamer with one of the largest tRNAs, despite each having a well-defined tertiary structure, enables the tRNA to perform live-cell protein synthesis in Escherichia coli. This tRNA–aptamer fusion can be switched on with quant ...
Nutrient Metabolism
... ABSTRACT A restricted protein diet supplemented with amino acids and fat may reduce the acidogenic effects of exercise. Twelve Arabian horses were assigned to a 2 ⫻ 2 factorial experiment: two fat levels: 0 or 10 g/100 g added corn oil and two crude protein levels: 7.5 g/100 g (supplemented with 0.5 ...
... ABSTRACT A restricted protein diet supplemented with amino acids and fat may reduce the acidogenic effects of exercise. Twelve Arabian horses were assigned to a 2 ⫻ 2 factorial experiment: two fat levels: 0 or 10 g/100 g added corn oil and two crude protein levels: 7.5 g/100 g (supplemented with 0.5 ...
Protein for Athletes
... of muscle, it is estimated that 10 to 14 g of additional protein is needed each day,2 although others dispute this claim. In addition to possible growth of muscle, protein is a highly versatile nutrient and is involved in other functions that are crucial to sports performance: cell regulation, muscl ...
... of muscle, it is estimated that 10 to 14 g of additional protein is needed each day,2 although others dispute this claim. In addition to possible growth of muscle, protein is a highly versatile nutrient and is involved in other functions that are crucial to sports performance: cell regulation, muscl ...
Fatty acid
... Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings ...
... Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings ...
Inflammation and ER Stress Regulate Branched
... epididymal adipose tissue of high-fat-fed mice but not in inguinal fat. To assess whether the down-regulation of the BCAA pathway gene expression was adipose specific, a panel of enzymes was profiled in brown fat, muscle, and liver, and expression was found to not be affected and, in the case of liv ...
... epididymal adipose tissue of high-fat-fed mice but not in inguinal fat. To assess whether the down-regulation of the BCAA pathway gene expression was adipose specific, a panel of enzymes was profiled in brown fat, muscle, and liver, and expression was found to not be affected and, in the case of liv ...
File
... the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most significant advance in the history of biology, with profound consequences for the future of our, an ...
... the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most significant advance in the history of biology, with profound consequences for the future of our, an ...
Tunicates and not cephalochordates are the closest living relatives
... support for Olfactores6,7. However, the extremely limited chordate species sampling considered in these studies prevented drawing any firm conclusions given its potentially deleterious effect on phylogenetic inference8. We have therefore extended the Philippe et al. dataset7 of 146 genes from four t ...
... support for Olfactores6,7. However, the extremely limited chordate species sampling considered in these studies prevented drawing any firm conclusions given its potentially deleterious effect on phylogenetic inference8. We have therefore extended the Philippe et al. dataset7 of 146 genes from four t ...
Biopathways Representation and Simulation on Hybrid Functional
... accepted to express biological phenomena such as biochemical reactions. But in this approach, it is rather difficult to observe the whole system intuitively like a picture if the system constitutes a large network of cascades. Although the discrete Petri net model allows very intuitive graphical repr ...
... accepted to express biological phenomena such as biochemical reactions. But in this approach, it is rather difficult to observe the whole system intuitively like a picture if the system constitutes a large network of cascades. Although the discrete Petri net model allows very intuitive graphical repr ...
Recombinant expression and characterisation of monofunctional S-
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
- Wiley Online Library
... Bioconversion of xylans has been intensively studied in the past decade because of its potential applications in agro-industrial processes, such as the pulp and paper industry and biofuel production. These studies have shown that xylan bioconversion is mediated by a wide array of enzymes (Collins et ...
... Bioconversion of xylans has been intensively studied in the past decade because of its potential applications in agro-industrial processes, such as the pulp and paper industry and biofuel production. These studies have shown that xylan bioconversion is mediated by a wide array of enzymes (Collins et ...
NUCLEOTIDES AND NUCLEIC ACIDS
... of a functional biological product, whether protein or RNA, is referred to as a gene. A cell typically has many thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broa ...
... of a functional biological product, whether protein or RNA, is referred to as a gene. A cell typically has many thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broa ...
GelRed™ Product Information Sheet
... acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have virtually the same spectra (Figure 1), so you can directly replace EtBr with GelRed without changing your existing imaging system. In ...
... acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have virtually the same spectra (Figure 1), so you can directly replace EtBr with GelRed without changing your existing imaging system. In ...
Analyzing Effects of Naturally Occurring Missense Mutations
... causes natural differences among races and ethnic populations, and also among healthy individuals and individuals susceptible to disease. On the DNA sequence level, the differences could be large or small, the smallest being a difference in a single-nucleotide. If such a difference occurs in some fracti ...
... causes natural differences among races and ethnic populations, and also among healthy individuals and individuals susceptible to disease. On the DNA sequence level, the differences could be large or small, the smallest being a difference in a single-nucleotide. If such a difference occurs in some fracti ...
Partial Class Notes Chapters 3 and 5 (4 slides/page)
... Average M.W. for an amino acid is ____________ so M.W. of most proteins is 5500 to 220,000 daltons. (One dalton equals one atomic mass unit; kilodalton = 1000 daltons). Most proteins have M.W. of 5.5-220 kd. Some proteins contain disulfide bonds that cross-link between _________residues by the oxida ...
... Average M.W. for an amino acid is ____________ so M.W. of most proteins is 5500 to 220,000 daltons. (One dalton equals one atomic mass unit; kilodalton = 1000 daltons). Most proteins have M.W. of 5.5-220 kd. Some proteins contain disulfide bonds that cross-link between _________residues by the oxida ...
BCMB 3100 – Chapter 3 (part 1)
... _______________________________________ (HIC) and _________________________ (RPC) are both based on interactions between hydrophobic patches on the surface of a protein and on the hydrophobicity of ligands (e.g. alkyl groups) covalently attached to a gel matrix. In RPC proteins can bind very strongl ...
... _______________________________________ (HIC) and _________________________ (RPC) are both based on interactions between hydrophobic patches on the surface of a protein and on the hydrophobicity of ligands (e.g. alkyl groups) covalently attached to a gel matrix. In RPC proteins can bind very strongl ...
Kein Folientitel
... “1. A DNA sequence for use in securing expression in a procaryotic or eucaryotic host cell of a polypeptide product having at least part of the primary structural confirmation [sic] of that of erythropoietin to allow possession of the biological property of causing bone marrow cells to increase pr ...
... “1. A DNA sequence for use in securing expression in a procaryotic or eucaryotic host cell of a polypeptide product having at least part of the primary structural confirmation [sic] of that of erythropoietin to allow possession of the biological property of causing bone marrow cells to increase pr ...
Anaerobic degradation of aromatic amino acids by
... and aromatic amino acids (the exceptions were valine, methionine, asparagine, aspartate and histidine) as a sole carbon and energy source. To the best of our knowledge, F. placidus is the first organism found to grow via anaerobic respiration with such a wide range of amino acids as the sole electro ...
... and aromatic amino acids (the exceptions were valine, methionine, asparagine, aspartate and histidine) as a sole carbon and energy source. To the best of our knowledge, F. placidus is the first organism found to grow via anaerobic respiration with such a wide range of amino acids as the sole electro ...
PPt Chapter 5 - columbusisd.org
... Concept 5.1: Macromolecules are polymers, built from monomers • A polymer is a long molecule consisting of many similar building blocks • These small building-block molecules are called monomers • Three of the four classes of life’s organic molecules are polymers: – Carbohydrates – Proteins – Nucle ...
... Concept 5.1: Macromolecules are polymers, built from monomers • A polymer is a long molecule consisting of many similar building blocks • These small building-block molecules are called monomers • Three of the four classes of life’s organic molecules are polymers: – Carbohydrates – Proteins – Nucle ...
The proton-linked monocarboxylate transporter (MCT) family
... velopment and in the adult chicken RPE [38]. These data imply regulation of MCT3 expression during development through the control of different promoters. Most recently, by searching the dbEST for fragments of MCTlike sequences, we identified four new potential members of the MCT family which were s ...
... velopment and in the adult chicken RPE [38]. These data imply regulation of MCT3 expression during development through the control of different promoters. Most recently, by searching the dbEST for fragments of MCTlike sequences, we identified four new potential members of the MCT family which were s ...
A Transient Expression Assay Using Maize Mesophyll Protoplasts
... plate reader if large number of samples are handled. General Comments In this system, photosynthetic gene promoters show tissue-specific and developmental (greening and normal green leaf development) regulation. In addition, regulated gene expression in response to metabolites, light, heat shock, ph ...
... plate reader if large number of samples are handled. General Comments In this system, photosynthetic gene promoters show tissue-specific and developmental (greening and normal green leaf development) regulation. In addition, regulated gene expression in response to metabolites, light, heat shock, ph ...
Lab: Colony PCR amplification of the 16S ribosomal RNA gene I
... Culture-independent techniques for characterizing microbial biodiversity are primarily based on the analysis of small subunit ribosomal RNA (SSU rRNA) genes from environmental samples (e.g. Giovannoni et al. 1990). This gene is an excellent phylogenetic marker for Bacteria and Archaea. With the aid ...
... Culture-independent techniques for characterizing microbial biodiversity are primarily based on the analysis of small subunit ribosomal RNA (SSU rRNA) genes from environmental samples (e.g. Giovannoni et al. 1990). This gene is an excellent phylogenetic marker for Bacteria and Archaea. With the aid ...
Classification and domain analysis of protein
... without gene IDs were removed from the dataset of each species. Next, proteins with known functions were separated for retrieval of protein kinases. For this, similar to human protein kinase dataset, protein coding genes from three genomes, which satisfy the presence of the three GO terms, i.e. ATP ...
... without gene IDs were removed from the dataset of each species. Next, proteins with known functions were separated for retrieval of protein kinases. For this, similar to human protein kinase dataset, protein coding genes from three genomes, which satisfy the presence of the three GO terms, i.e. ATP ...
Plasma membrane
... doublets in a 9 2 array (not shown in the model cell) Function Movement of material over cell surface ...
... doublets in a 9 2 array (not shown in the model cell) Function Movement of material over cell surface ...
R26 :: CAG GCaMP6f - The Jackson Laboratory
... international biomedical research community - adds hundreds of new strains annually. The JAX Mouse Repository is supported by NIH, The Howard Hughes Medical Institute and several private charitable foundations. ...
... international biomedical research community - adds hundreds of new strains annually. The JAX Mouse Repository is supported by NIH, The Howard Hughes Medical Institute and several private charitable foundations. ...
The Long Noncoding RNA CHRF Regulates Cardiac Hypertrophy
... response of cardiomyocytes partially through a toll-like receptor 4–mediated, Myd88-dependent nuclear factor-κB (NF-κB) pathway.14 However, it is not yet clear whether Myd88 is a target of miRNAs in the hypertrophic machinery. Long noncoding RNAs (lncRNAs) are transcribed RNA molecules >200 nucleot ...
... response of cardiomyocytes partially through a toll-like receptor 4–mediated, Myd88-dependent nuclear factor-κB (NF-κB) pathway.14 However, it is not yet clear whether Myd88 is a target of miRNAs in the hypertrophic machinery. Long noncoding RNAs (lncRNAs) are transcribed RNA molecules >200 nucleot ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.