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From Sequence to Structure
From Sequence to Structure

... isolation, or the cellular function it performs as part of an assemblage or complex with other molecules, or the phenotype it produces in the cell or organism. Major examples of the biochemical functions of proteins include binding; catalysis; operating as molecular switches; and serving as structur ...
Annex 1
Annex 1

... (iv) "amino acids" are those L-amino acids commonly found in naturally occurring proteins and are listed in paragraph 48, table 3. Those amino acid sequences containing at least one D-amino acid are not intended to be embraced by this definition. Any amino acid sequence that contains post-translatio ...
VTC4 Is a Bifunctional Enzyme That Affects
VTC4 Is a Bifunctional Enzyme That Affects

... route to produce myoinositol in what has been referred to as the Loewus pathway (Eisenberg et al., 1964; Chen and Charalampous, 1966; Sherman et al., 1969; Loewus and Loewus, 1980; Loewus et al., 1980). The conversion of Glc-6-P to InsP is catalyzed by the myoinositol phosphate synthase (EC 5.5.1.4; ...
View/Open - Indiana University
View/Open - Indiana University

... lengthening it or shortening it. “Pairs” here refers to a string – or a substring – of the initial string used as the search string – and one or more strings or substrings found in a database. The search starts with the creation of all possible subwords of a given length (default typically 11 for DN ...
Lo, Frederick: Three Recently Developed Algorithms for Aligning Distantly Related Proteins
Lo, Frederick: Three Recently Developed Algorithms for Aligning Distantly Related Proteins

... some differences. The sequence-sequence similarity matrix can apparently be constructed from a substitution matrix and dynamic programming. Like an amino-acid substitution matrix, the similarity matrix measures similarity between pairs of query and template residues. However, while a high value in ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
Comparison of the Structure of the Extrinsic 33 kDa Protein from

... mature protein; they are located at positions 100 and 193, respectively. Among them, the ATG codon at nucleotide number 100 was assigned as the start codon, because this codon is similar to the start codon (AnnATGGC) for translation initiation in higher plants (Lutcke et al. 1987) (see also below). ...
Document
Document

... different components of the body[2]. Of course, the contribution of these components to whole body protein content differs with carcass protein being the largest contributor to whole body protein. Also, the contribution of the different parts to whole body protein changes over time with an increasin ...
Protein Sequences in the CAS Registry File on
Protein Sequences in the CAS Registry File on

... • Sequences deduced from gene translation and reported by the author • Sequences deduced by gene translation from the GenBank® database (registered trademark of the U.S. Department of Health and Human Services) • Chemically modified peptides and proteins • Genetically engineered and synthetic protei ...
Identification of a Cis-Acting Element of ART1, a
Identification of a Cis-Acting Element of ART1, a

... able to bind ART1 protein (Fig. 2B). These results indicate that the region between 2358 and 2319 in the STAR1 promoter contains cis-acting element of ART1. This is further confirmed by competition and super shift experiments (Fig. 2C, lanes 5–8). The candidate region was further narrowed by gelshif ...
Types and effects of protein variations. Vihinen
Types and effects of protein variations. Vihinen

... “Protein truncation” is a special case of deletion where deletion occurs either in N- or Cterminus. Indels originate due to both insertion and deletion. The different types of protein variations in a short protein sequence for alanine, alanine, serine… in one letter code AASEQWENCE are depicted in F ...
Observations on the role of nuclear medicine in molecular imaging
Observations on the role of nuclear medicine in molecular imaging

... completely new spectrum of targets for molecular imaging. Among these targets, the first to receive attention is genetic expression. In fact, the term ‘‘molecular genetic imaging’’ has been proposed as a subfield of molecular imaging [Blasberg and Tjuvajev, 2002]. However, it is appropriate to point ...
Pseudomon-1 motif
Pseudomon-1 motif

... decision in the present work to group bacteria at the level of order, rather than the more-broad phylum or class, also might help to reduce spurious sequences in clusters. 3. The use of environmental sequences helped to find RNAs that are not well represented in organisms whose genomes have been fu ...
Prying Into Prions - Colorado Parks and Wildlife
Prying Into Prions - Colorado Parks and Wildlife

... which is short for “proteinaceaous infectious particle.” The prion protein exists in two forms. The normal form is found in many types of cells, including those in the central nervous system. The pathological prion form has the same chemical composition, but a different shape. This abnormally shaped ...
NSPKU
NSPKU

... • A protein substitute contains NO Phe... But it contains all of the other amino acids in protein that your child needs. • Most protein substitutes also contain vitamins, minerals and other important nutrients to ensure your child is getting what they need. The protein substitute is an extremely imp ...
SPECIFIC PROTEIN SYNTHESIS IN CELLULAR
SPECIFIC PROTEIN SYNTHESIS IN CELLULAR

... laterally so as to form voluminous channels for yolk transport (King and Aggarwal, 1965) . In addition, the cells synthesize and secrete into the channels a macromolecule, probably a glycoprotein, which finds its way into the yolk spheres of the oocyte (Anderson and Telfer, 1969) . From experiments ...
Modifying the chain-length selectivity of the
Modifying the chain-length selectivity of the

... Results and discussion With increasing knowledge about the three-dimensional structures of lipases, it has become possible to explain some of the enzyme properties based on their structures. For example, the interfacial activation of lipase is revealed by the presence of a lid (or flap) structure an ...
Limits of adaptation to high dietary protein intakes
Limits of adaptation to high dietary protein intakes

... Activity=inactivity (level and type) ...
Identification of a Protein with Antioxidant Activity that is Important for
Identification of a Protein with Antioxidant Activity that is Important for

... Beer flavour is determined by its chemical composition, which includes proteins and volatile flavour compounds such as esters, alcohols, fatty acids, sulphur compounds and ketones. The stability of these chemical components determines the shelf life of packaged beer. As the exportation of beer conti ...
Functional characterization of polypeptide release factor 1b in the
Functional characterization of polypeptide release factor 1b in the

... The read-through assay was performed to address why Eob/Sc eRF1 could not support the viability of the above yeast cells. The hybrid gene Eob/Sc eRF1 was transformed into yeast strain YDB447 containing the support plasmid pDB967 (wild-type SUP45) and a dual luciferase reporter (the Firefly and Renil ...
Genbiotech Store
Genbiotech Store

... acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have virtually the same spectra (Figure 1), so you can directly replace EtBr with GelRed without changing your existing imaging system. In ...
Characteristics of the gene encoding pyrroline-5-carboxylate synthase (P5CS) in Glycine max
Characteristics of the gene encoding pyrroline-5-carboxylate synthase (P5CS) in Glycine max

... osmotic pressure in plant cells is adjusted through the synthesis and accumulation of substances such as sugar exhange, polyamine, Betaine glycine, proline... Among these substances, proline is an exchange factor which has been studied in detail. Proline is known as one of the substances playing an ...
OPTIMIZER: a web server for optimizing the codon usage of DNA
OPTIMIZER: a web server for optimizing the codon usage of DNA

... highly expressed genes usually use the minimal subset of codons that are recognized by the most abundant tRNA species, their ENc values are expected to be low. Figure 1 shows some of the outputs provided by the optimization of a DNA sequence: for example, the query and optimized sequences and an ali ...
De Novo Pyrimidine Nucleotide Synthesis Mainly
De Novo Pyrimidine Nucleotide Synthesis Mainly

... to the Nucleobase:Cation Symporter1 (NCS1) family of transport proteins, also known as purine-related transporters (Saier et al., 2009; www.tcdb.org). The closest Arabidopsis homolog to this protein is encoded by At5g03555. The deduced amino acid sequence exhibits 23% identity and 43% similarity to ...
Biochemical Oscillations
Biochemical Oscillations

... (either its affinity for substrates or its rate of converting bound substrates into products). Such enzymes are called “allosteric” because in addition to substrate-binding sites, they have “other sites” for binding regulatory molecules that either activate or inhibit the enzyme. Allosteric proteins ...
article in press - Biochemistry
article in press - Biochemistry

... the known differences). However, all of the known AMPK complexes are activated both by phosphorylation by an upstream kinase and allosterically by AMP. Phosphorylation of a threonine residue within the activation loop of the asubunit (T172) is required for kinase activity (Hawley et al., 1996; Stein ...
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Gene expression



Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.
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