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Disorder Prediction Methods, Their Applicability to Different Protein
... to investigate any disorder. It was predicted that the C-terminal 106 residues were indeed disordered, however when >100 residues were removed, protein expression was negligible. A construct excluding the C-terminal 100 residues was ultimately chosen and successfully crystallised [20]. This example ...
... to investigate any disorder. It was predicted that the C-terminal 106 residues were indeed disordered, however when >100 residues were removed, protein expression was negligible. A construct excluding the C-terminal 100 residues was ultimately chosen and successfully crystallised [20]. This example ...
The potato tuber mitochondrial proteome
... growing and dividing, and as such they perform DNA replication, DNA transcription, ...
... growing and dividing, and as such they perform DNA replication, DNA transcription, ...
Safety assessment - Food Standards Australia New Zealand
... FSANZ’s role is to protect the health and safety of people in Australia and New Zealand through the maintenance of a safe food supply. FSANZ is a partnership between ten Governments: the Commonwealth; Australian States and Territories; and New Zealand. It is a statutory authority under Commonwealth ...
... FSANZ’s role is to protect the health and safety of people in Australia and New Zealand through the maintenance of a safe food supply. FSANZ is a partnership between ten Governments: the Commonwealth; Australian States and Territories; and New Zealand. It is a statutory authority under Commonwealth ...
Sequence-based predictions of membrane-protein topology, homology and insertion
... do not require data from expensive biochemical experiments, but also for the prospect that the wealth of training data can only be expected to increase. Development of such sequence based prediction methods is only becoming increasingly important as the sequence collection continues to grow. In part ...
... do not require data from expensive biochemical experiments, but also for the prospect that the wealth of training data can only be expected to increase. Development of such sequence based prediction methods is only becoming increasingly important as the sequence collection continues to grow. In part ...
Two Oxidosqualene Cyclases Responsible for
... isomers, a reaction catalyzed by oxidosqualene cyclases (OSCs). The different OSCs have characteristic product specificities, which are mainly due to differences in the numbers of high-energy intermediates the enzymes can stabilize. The goal of this investigation was to clone and characterize OSCs f ...
... isomers, a reaction catalyzed by oxidosqualene cyclases (OSCs). The different OSCs have characteristic product specificities, which are mainly due to differences in the numbers of high-energy intermediates the enzymes can stabilize. The goal of this investigation was to clone and characterize OSCs f ...
Biochemical Evidence for the Role of the Waxy Protein fron Pea
... eluted at the same positions in the gradient (not illustrated). Another factor that did not affect recovery of activity or the activity profile obtained from the Mono-Q chromatography was whether the purified starch was used 'wet/ i.e. solubilized immediately after its purification, or whether it wa ...
... eluted at the same positions in the gradient (not illustrated). Another factor that did not affect recovery of activity or the activity profile obtained from the Mono-Q chromatography was whether the purified starch was used 'wet/ i.e. solubilized immediately after its purification, or whether it wa ...
Effects of Long-Term Thyroid Hormone Level Alterations, n
... already used in human therapy. We applied pravastatin beginning from the 4th postnatal week for maximally 21 months. Comparing the life span of rat and man, this corresponds to the administration in man from about the age of 3 to 75 years. The minimal dose was 50 μg/100 g at the beginning of the exp ...
... already used in human therapy. We applied pravastatin beginning from the 4th postnatal week for maximally 21 months. Comparing the life span of rat and man, this corresponds to the administration in man from about the age of 3 to 75 years. The minimal dose was 50 μg/100 g at the beginning of the exp ...
Molecular Characterisation of the 76 kDa Iron
... and partially sequenced. The largest was sequenced in its full length. The amino acid sequence deduced from the cDNA matches the peptide sequences from the isolated subunit, apart from an additional Ala residue, not seen in the protein sequence (Fig. 1). The first ATG triplet is preceded by an in-fr ...
... and partially sequenced. The largest was sequenced in its full length. The amino acid sequence deduced from the cDNA matches the peptide sequences from the isolated subunit, apart from an additional Ala residue, not seen in the protein sequence (Fig. 1). The first ATG triplet is preceded by an in-fr ...
A Novel Role for Vitamin K1 in a Tyrosine Phosphorylation
... type III and immunoglobulin motifs common to extracellular matrix proteins, neural cell adhesion molecules, and cell surface receptors with tyrosine kinase or phosphatase activities (13, 14). It is believed that these RTKs may be bifunctional, acting both as cell adhesion proteins and as components ...
... type III and immunoglobulin motifs common to extracellular matrix proteins, neural cell adhesion molecules, and cell surface receptors with tyrosine kinase or phosphatase activities (13, 14). It is believed that these RTKs may be bifunctional, acting both as cell adhesion proteins and as components ...
Full-Text PDF
... soluble solids (TSS) and titratable acidity (TA), respectively [5], and the relationship between TSS and TA plays an important role in consumer acceptance of several species, such as citrus [6], table grapes [7] and cherries [8]. In fruit species such as apples, grapes and tomatoes, acidity has long ...
... soluble solids (TSS) and titratable acidity (TA), respectively [5], and the relationship between TSS and TA plays an important role in consumer acceptance of several species, such as citrus [6], table grapes [7] and cherries [8]. In fruit species such as apples, grapes and tomatoes, acidity has long ...
View/Open - VUW research archive - Victoria University of Wellington
... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
A wide-range phylogenetic analysis of Zic proteins: Implications for
... From our current results, we can envisage the evolutionary process of Zic genes as follows (Fig. 5A). The earliest Zic genes that appeared in ancestral Eumetazoa or metazoans, probably in the pre-Cambrian era, may have shared common structural features, namely the ZF and ZF-NC domains. Exon–intron b ...
... From our current results, we can envisage the evolutionary process of Zic genes as follows (Fig. 5A). The earliest Zic genes that appeared in ancestral Eumetazoa or metazoans, probably in the pre-Cambrian era, may have shared common structural features, namely the ZF and ZF-NC domains. Exon–intron b ...
Passenger Protein Determines Translocation Versus Retention in
... lumen, integral membrane proteins embed in the ER membrane through one or more of their transmembrane segments. Furthermore, N-glycosylation and signal peptide cleavage are cotranslational modifications of proteins in the ER, performed by oligosaccharyltransferase (OST) and signal peptidase complex, ...
... lumen, integral membrane proteins embed in the ER membrane through one or more of their transmembrane segments. Furthermore, N-glycosylation and signal peptide cleavage are cotranslational modifications of proteins in the ER, performed by oligosaccharyltransferase (OST) and signal peptidase complex, ...
Pseudouridine at position 55 in tRNA controls the contents of other
... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
Flavors of Protein Disorder
... can depend on a structural ensemble rather than a unique 3-D structure was suggested more than 50 years ago7, and that some proteins don’t fold due to an atypical amino acid composition was suggested more than 20 years ago8, 9. More recently, such proteins have been called "natively unfolded"10, "in ...
... can depend on a structural ensemble rather than a unique 3-D structure was suggested more than 50 years ago7, and that some proteins don’t fold due to an atypical amino acid composition was suggested more than 20 years ago8, 9. More recently, such proteins have been called "natively unfolded"10, "in ...
- Department of Biosystems Science and
... The molecular chaperone heat-shock protein of 90 kDa (Hsp90) stabilizes various proteins and occupies a central position in cellular networks. Hsp90 inhibitors are being tested in clinical trials as anticancer drugs. Recent studies have illuminated the unappreciated significance of this chaperone in ...
... The molecular chaperone heat-shock protein of 90 kDa (Hsp90) stabilizes various proteins and occupies a central position in cellular networks. Hsp90 inhibitors are being tested in clinical trials as anticancer drugs. Recent studies have illuminated the unappreciated significance of this chaperone in ...
Chapter 6
... organized up-side down when compared to deuterostomes, such as vertebrates. If this is true, then their respective ventrally (belly-side) and dorsally (back-side) located nervous system are homologous (inherited from a common ancestor, Arendt and Nübler-Jung 1994). Although it has been argued that a ...
... organized up-side down when compared to deuterostomes, such as vertebrates. If this is true, then their respective ventrally (belly-side) and dorsally (back-side) located nervous system are homologous (inherited from a common ancestor, Arendt and Nübler-Jung 1994). Although it has been argued that a ...
Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid
... Background: Due to high neutral lipids accumulation in the cytoplasm, in vitro-produced embryos from Bos primigenius indicus and their crosses are more sensitive to chilling and cryopreservation than those from Bos primigenius taurus. The objective of the present study was to evaluate the effects of ...
... Background: Due to high neutral lipids accumulation in the cytoplasm, in vitro-produced embryos from Bos primigenius indicus and their crosses are more sensitive to chilling and cryopreservation than those from Bos primigenius taurus. The objective of the present study was to evaluate the effects of ...
Evolutionary Patterns in the Sequence and Structure of
... Transfer RNAs (tRNAs) are ancient molecules that are central to translation. Since they probably carry evolutionary signatures that were left behind when the living world diversified, we reconstructed phylogenies directly from the sequence and structure of tRNA using well-established phylogenetic me ...
... Transfer RNAs (tRNAs) are ancient molecules that are central to translation. Since they probably carry evolutionary signatures that were left behind when the living world diversified, we reconstructed phylogenies directly from the sequence and structure of tRNA using well-established phylogenetic me ...
University of Groningen Stress responses and sugar metabolism in
... In addition to these phosphoproteins also low molecular weight molecules such as NADP, glucose‐6‐phosphate (G6P) and fructose‐1,6‐bisphosphate (FBP) can modulate either DNA‐ binding properties or the interaction with the transcriptional machinery of the CcpA‐(HPr‐ Ser ...
... In addition to these phosphoproteins also low molecular weight molecules such as NADP, glucose‐6‐phosphate (G6P) and fructose‐1,6‐bisphosphate (FBP) can modulate either DNA‐ binding properties or the interaction with the transcriptional machinery of the CcpA‐(HPr‐ Ser ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
Pathways to abscisic acid-regulated gene expression
... 2000). DET1 encodes a nuclear-localized protein that when mutated results in severe defects in the temporal and spatial regulation of light-regulated and developmental (e.g. ABI3) genes. The CO gene encodes a protein with homology to zinc-finger transcription factors that promotes flowering (Putteri ...
... 2000). DET1 encodes a nuclear-localized protein that when mutated results in severe defects in the temporal and spatial regulation of light-regulated and developmental (e.g. ABI3) genes. The CO gene encodes a protein with homology to zinc-finger transcription factors that promotes flowering (Putteri ...
Chapter 5
... component in animal cell membranes • Although cholesterol is essential in animals, high levels in the blood may contribute to cardiovascular disease ...
... component in animal cell membranes • Although cholesterol is essential in animals, high levels in the blood may contribute to cardiovascular disease ...
Research Resources: Comparative MMM icroRNA
... biosynthesis, the regulation of meiosis, gene transcription, and by protecting the oocytes [8–10]. Recently, a type of post-transcriptional regulator, microRNA (miRNA), has received wide-spread attention in ovarian granulosa cells during folliculogenesis [11–13]. miRNAs are endogenous non-coding RNA ...
... biosynthesis, the regulation of meiosis, gene transcription, and by protecting the oocytes [8–10]. Recently, a type of post-transcriptional regulator, microRNA (miRNA), has received wide-spread attention in ovarian granulosa cells during folliculogenesis [11–13]. miRNAs are endogenous non-coding RNA ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.