The Protein Cevalently Linked to the 5'... of Poliovirus RNA by Victor Robert Ambros
... D. Kinetics of removal of VPg from poliovirus RNA in Hela cell extracts........................................ 84 E. Release of VPg from polio RNA in other cell extracts..... 88 F. Glycerol gradient sedimentation of Hela unlinking activity.................................................. 91 G. Mag ...
... D. Kinetics of removal of VPg from poliovirus RNA in Hela cell extracts........................................ 84 E. Release of VPg from polio RNA in other cell extracts..... 88 F. Glycerol gradient sedimentation of Hela unlinking activity.................................................. 91 G. Mag ...
A complete shikimate pathway in Toxoplasma gondii: an ancient
... polypeptide to have been studied in depth, we have compared the DHQ synthase domains from both the T. gondii and E. nidulans AROMs to determine if the key features are conserved between both proteins (Fig. 1D). All the key residues identified by Carpenter et al. (1998) which are known to be involved ...
... polypeptide to have been studied in depth, we have compared the DHQ synthase domains from both the T. gondii and E. nidulans AROMs to determine if the key features are conserved between both proteins (Fig. 1D). All the key residues identified by Carpenter et al. (1998) which are known to be involved ...
Relationship between Human Adipose Tissue Agouti and - Zen-Bio
... Fatty acid synthase is the key enzyme in de novo lipogenesis. Both human liver and adipose tissue exhibit substantially lower FAS activity than that found in rats (Weiss et al. 1986). Nonetheless, significant de novo lipogenesis is well documented in human adipocytes. Human adipocytes contain substa ...
... Fatty acid synthase is the key enzyme in de novo lipogenesis. Both human liver and adipose tissue exhibit substantially lower FAS activity than that found in rats (Weiss et al. 1986). Nonetheless, significant de novo lipogenesis is well documented in human adipocytes. Human adipocytes contain substa ...
Probabilistic Reconciliation Analysis for Genes and Pseudogenes
... and future directions are given in Chapter 5. ...
... and future directions are given in Chapter 5. ...
Chaperone-dependent gene expression of organic
... position, which serves as a check on the location and accuracy of the protein-coding sequence. Most work has reported high G + C contents of Pseudomonas sp. lipase genes [13,19,20]. According to West and Iglewski [21], a high G + C content is typical for DNA of P. aeruginosa. The high G + C content ...
... position, which serves as a check on the location and accuracy of the protein-coding sequence. Most work has reported high G + C contents of Pseudomonas sp. lipase genes [13,19,20]. According to West and Iglewski [21], a high G + C content is typical for DNA of P. aeruginosa. The high G + C content ...
Regulation of metabolism by dietary carbohydrates in two lines of
... acids, relatively low number of insulin receptors, lack of inhibition of endogenous glucose production and poor hepatic lipogenesis from glucose were proved to be true (Mommsen and Plisetskaya, 1991; Hemre and Kahrs, 1997; Navarro et al., 1999; Panserat et al., 2000b; Enes et al., 2009); reduced per ...
... acids, relatively low number of insulin receptors, lack of inhibition of endogenous glucose production and poor hepatic lipogenesis from glucose were proved to be true (Mommsen and Plisetskaya, 1991; Hemre and Kahrs, 1997; Navarro et al., 1999; Panserat et al., 2000b; Enes et al., 2009); reduced per ...
Colicins produced by the Escherichia fergusonii strains closely
... EF3) was isolated after insertion of Tn7 into the plasmid backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 pr ...
... EF3) was isolated after insertion of Tn7 into the plasmid backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 pr ...
Phylogeny of Firmicutes with special reference to Mycoplasma
... The phylogenetic position of Thermotoga within the Firmicutes was unexpected. The Pgk from Thermotoga was included in the analysis only because of its high sequence similarity, obtained by BLAST search, to Firmicutes Pgk sequences. Thermotoga has an affinity to the low-G+C, Gram-positive Bacteria (N ...
... The phylogenetic position of Thermotoga within the Firmicutes was unexpected. The Pgk from Thermotoga was included in the analysis only because of its high sequence similarity, obtained by BLAST search, to Firmicutes Pgk sequences. Thermotoga has an affinity to the low-G+C, Gram-positive Bacteria (N ...
Full-Text PDF
... have found that the triolide of R-3HB forms crown ester complexes with alkali metals; Burger and Seebach [39] have shown that oligomers of R-3HB transport alkali and alkaline earth salts across methylene chloride layers in U-tubes; Seebach et al. [40] have demonstrated that PHB of 16 residues or mul ...
... have found that the triolide of R-3HB forms crown ester complexes with alkali metals; Burger and Seebach [39] have shown that oligomers of R-3HB transport alkali and alkaline earth salts across methylene chloride layers in U-tubes; Seebach et al. [40] have demonstrated that PHB of 16 residues or mul ...
Study of Different Variants of Mo Enzyme crARC and the Interaction
... the human and Chlamydomonas system [15,17]. We performed a series of experiments to verify if the activity of the main known Mo enzymes are very short at room temperatures [23]. Therefore, in there is indeed a real protein complex between these three proteins, to know its stoichiometry and compariso ...
... the human and Chlamydomonas system [15,17]. We performed a series of experiments to verify if the activity of the main known Mo enzymes are very short at room temperatures [23]. Therefore, in there is indeed a real protein complex between these three proteins, to know its stoichiometry and compariso ...
Transcriptional Regulation of Metabolism
... study of metabolism. It revealed how organisms can adapt their metabolic activity to environmental nutritional changes by modifying the level of expression of specific enzymes and linked modulation of enzymatic activity to the transcriptional control of gene expression for the first time. It is now ...
... study of metabolism. It revealed how organisms can adapt their metabolic activity to environmental nutritional changes by modifying the level of expression of specific enzymes and linked modulation of enzymatic activity to the transcriptional control of gene expression for the first time. It is now ...
Journal of Biotechnology
... Despite their structural heterogeneity, non-ribosomal peptide antibiotics share a common mode of synthesis, the multicarrier thiotemplate mechanism (Stein et al., 1996). They are biosynthesized by multi-modular proteins termed non-ribosomal peptide synthetases. Each elongation cycle in non-ribosomal ...
... Despite their structural heterogeneity, non-ribosomal peptide antibiotics share a common mode of synthesis, the multicarrier thiotemplate mechanism (Stein et al., 1996). They are biosynthesized by multi-modular proteins termed non-ribosomal peptide synthetases. Each elongation cycle in non-ribosomal ...
Text - Enlighten: Publications
... production of mevalonic acid from HMG-CoA by HMG-CoA reductase, the rate-limiting step in the cholesterol synthesis pathway and the target for statins. Following a combination of phosphorylation and decarboxylation reactions, mevalonate gives rise to isopentenyl pyrophosphate (IPP), which is then co ...
... production of mevalonic acid from HMG-CoA by HMG-CoA reductase, the rate-limiting step in the cholesterol synthesis pathway and the target for statins. Following a combination of phosphorylation and decarboxylation reactions, mevalonate gives rise to isopentenyl pyrophosphate (IPP), which is then co ...
Molecular characterization of carnitine palmitoyltransferase 1C Esther Gratacòs i Batlle
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
Identity elements in tRNA-mediated transcription
... Fig. 3. Effect of native and chimeric tRNAs on transcription antitermination in the trpE-lacZ fusion. RNA was isolated from L. lactis cells after a growth medium shift as indicated in Methods and used to prepare a Northern blot that was hybridized with a lacZ-specific probe to visualize full length ...
... Fig. 3. Effect of native and chimeric tRNAs on transcription antitermination in the trpE-lacZ fusion. RNA was isolated from L. lactis cells after a growth medium shift as indicated in Methods and used to prepare a Northern blot that was hybridized with a lacZ-specific probe to visualize full length ...
Sink regulation of photosynthesis
... centre proteins (Pfannschmidt et al., 1999). This redox regulatory system controlling chloroplast transcription appears to have been conserved through evolution from the prokaryotic ancestor. Also important are the redox signals that originate in the chloroplast and are transported to other compartm ...
... centre proteins (Pfannschmidt et al., 1999). This redox regulatory system controlling chloroplast transcription appears to have been conserved through evolution from the prokaryotic ancestor. Also important are the redox signals that originate in the chloroplast and are transported to other compartm ...
Time-Resolved Transcriptome Analysis of Bacillus subtilis
... amino acids and oligopeptides and constitutes a rich nitrogen source, were used. The products of CAA-regulated genes are involved in various metabolic pathways, including nitrogen metabolism [16,17], the development of competence [18], and chemotaxis [19]. The CodY protein has been found to play a k ...
... amino acids and oligopeptides and constitutes a rich nitrogen source, were used. The products of CAA-regulated genes are involved in various metabolic pathways, including nitrogen metabolism [16,17], the development of competence [18], and chemotaxis [19]. The CodY protein has been found to play a k ...
The Organellar Genome and Metabolic Potential
... in various species (Gabaldón and Huynen 2004). Within the evolutionary ‘‘gap’’ between species with genome-containing mitochondria and species with mitosomes or hydrogenosomes (both lacking an organellar genome), the species N. ovalis provides an interesting link. Nyctotherus ovalis is an anaerobic ...
... in various species (Gabaldón and Huynen 2004). Within the evolutionary ‘‘gap’’ between species with genome-containing mitochondria and species with mitosomes or hydrogenosomes (both lacking an organellar genome), the species N. ovalis provides an interesting link. Nyctotherus ovalis is an anaerobic ...
The Amino Acid Sequences of the Myelin
... expression library. Analysis of the primary amino acid sequences reveals several features of these proteins that may be related to their postulated function as glial-neuron recognition molecules. These include: (a) the tripeptide sequence Arg-Gly-Asp (RGD), which has been found to mediate binding in ...
... expression library. Analysis of the primary amino acid sequences reveals several features of these proteins that may be related to their postulated function as glial-neuron recognition molecules. These include: (a) the tripeptide sequence Arg-Gly-Asp (RGD), which has been found to mediate binding in ...
Eds., M. Kawaguchi, K. Misaki, H. Sato, T. Yokokawa, T.... and S. Tanabe, pp. 35–40.
... damage by multiple enzymatic mechanisms and by antioxidant molecules. The SODs are the first and most important line of antioxidant enzyme defense against oxidative stress, such as that caused by superoxide anion radicals. We examined the in vitro effects of taurine on the SOD activity levels in the ...
... damage by multiple enzymatic mechanisms and by antioxidant molecules. The SODs are the first and most important line of antioxidant enzyme defense against oxidative stress, such as that caused by superoxide anion radicals. We examined the in vitro effects of taurine on the SOD activity levels in the ...
University: Suez Canal University Faculty of Medicine Course
... 10. Describe in details and with formulas the following reactions including their cofactors and their relationship to the removal of nitrogen waste in the body: transaminase, glutamate dehydrogenase, glutamine synthetase and glutaminase. 11. In relation to the urea cycle: Describe in details using f ...
... 10. Describe in details and with formulas the following reactions including their cofactors and their relationship to the removal of nitrogen waste in the body: transaminase, glutamate dehydrogenase, glutamine synthetase and glutaminase. 11. In relation to the urea cycle: Describe in details using f ...
Review of Osmotic Pressure Driven Release of Proteins from
... factors affecting these changes are mechanical forces such as shear, the presence of surfactants, buffers, ionic strength, the presence of oxidizers such as ions, radicals and peroxide, light, pH and temperature. Denaturation of the protein molecule may result in a loss of its activity or may make t ...
... factors affecting these changes are mechanical forces such as shear, the presence of surfactants, buffers, ionic strength, the presence of oxidizers such as ions, radicals and peroxide, light, pH and temperature. Denaturation of the protein molecule may result in a loss of its activity or may make t ...
Structural Basis of Biological Nitrogen Fixation
... component nitrogenase proteins from both Azotobacter vinelandii and Clostridium pasteurianum.30-35 The sequence, mutagenesis, and structural data provide the foundation for formulating an initial picture of the nitrogenase mechanism at the molecular level. Although nitrogen fixation is a property of ...
... component nitrogenase proteins from both Azotobacter vinelandii and Clostridium pasteurianum.30-35 The sequence, mutagenesis, and structural data provide the foundation for formulating an initial picture of the nitrogenase mechanism at the molecular level. Although nitrogen fixation is a property of ...
Characterization of Low Molecular Weight Glutenin Subunits by
... cysteines in parts of the molecule yet to be sequenced that make them chain extenders? This question can only be answered by future work, because almost no information about the arrangement of disulfide linkages in the gluten proteins exists despite the key role they play in determining the properti ...
... cysteines in parts of the molecule yet to be sequenced that make them chain extenders? This question can only be answered by future work, because almost no information about the arrangement of disulfide linkages in the gluten proteins exists despite the key role they play in determining the properti ...
Comparison of Sample Sequences of the Genome to the Sequence K-12
... less significant than 1e25, a level at which the significance of any alignments are unreliable. These entirely novel sequences of no known function which occur in S. typhi but not E. coli K-12 presumably include some genes encoding novel functions. Three-way comparisons. Pairwise comparisons of Blas ...
... less significant than 1e25, a level at which the significance of any alignments are unreliable. These entirely novel sequences of no known function which occur in S. typhi but not E. coli K-12 presumably include some genes encoding novel functions. Three-way comparisons. Pairwise comparisons of Blas ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.