Influence of the regulatory protein RsmA on cellular
... (Goodman et al., 2004). Interestingly, these phenotypes were suppressed by transposon insertions in gacS, gacA and rsmB. The authors propose that, under conditions of acute infection, environmental signals favour activation of RetS, resulting in repression of the GacS/GacA/RsmB pathway. A mutation i ...
... (Goodman et al., 2004). Interestingly, these phenotypes were suppressed by transposon insertions in gacS, gacA and rsmB. The authors propose that, under conditions of acute infection, environmental signals favour activation of RetS, resulting in repression of the GacS/GacA/RsmB pathway. A mutation i ...
Proteomic analyses of Oryza sativa mature pollen reveal novel
... polarity and cell recognition of pollen-stigma, and initiation of a hierarchical signal cascade. Recently, several proteins identified in the mature pollen of some species have been shown to be involved in hydration, cohesion and cell recognition of pollen-stigma [2–4]. The importance of tip-focused ...
... polarity and cell recognition of pollen-stigma, and initiation of a hierarchical signal cascade. Recently, several proteins identified in the mature pollen of some species have been shown to be involved in hydration, cohesion and cell recognition of pollen-stigma [2–4]. The importance of tip-focused ...
C urrent and prospective applications of metal ion–protein
... techniques and their implications in life sciences. ...
... techniques and their implications in life sciences. ...
A Metabolic Node in Action: Chorismate
... that led to the synthesis of an extensive range of products. This pathway is present only in bacteria, fungi, and plants. While there is only little difference in the sequence of the chemical reactions of the pathway, significant differences exist in terms of organization and regulation. In the main ...
... that led to the synthesis of an extensive range of products. This pathway is present only in bacteria, fungi, and plants. While there is only little difference in the sequence of the chemical reactions of the pathway, significant differences exist in terms of organization and regulation. In the main ...
Interpreting the Genetic Code
... The net effect of wobble base pairing is to reduce the number of tRNAs that must be produced by a cell In reality cells do not make 61 different tRNAs, one for each codon Many tRNAs have anticodons that anneal to several different codons\ Codons are known for which there are more than one tRNA, alth ...
... The net effect of wobble base pairing is to reduce the number of tRNAs that must be produced by a cell In reality cells do not make 61 different tRNAs, one for each codon Many tRNAs have anticodons that anneal to several different codons\ Codons are known for which there are more than one tRNA, alth ...
Whole-tree and tension wood-associated expression profiles of microRNAs in Eucalyptus Grant R. McNair
... components, in this case miRNAs. In order to develop a more complete understanding of miRNA function in wood formation, one should also take into account the complexities of plant development as a whole. The aim of this M.Sc study is the determination of miRNA abundance patterns at the whole tree le ...
... components, in this case miRNAs. In order to develop a more complete understanding of miRNA function in wood formation, one should also take into account the complexities of plant development as a whole. The aim of this M.Sc study is the determination of miRNA abundance patterns at the whole tree le ...
patrick_tb_ch17
... Feedback: Both hydroxy groups are involved in hydrogen bonding interactions, but one of the hydroxy groups is more involved than the other. Removing one of the OH groups results in less binding interactions but there is a net gain in binding stability since less energy is required to desolvate one h ...
... Feedback: Both hydroxy groups are involved in hydrogen bonding interactions, but one of the hydroxy groups is more involved than the other. Removing one of the OH groups results in less binding interactions but there is a net gain in binding stability since less energy is required to desolvate one h ...
Cloning, Sequencing, and Characterization of Luciola italica
... comparisons among firefly species can be used to determine important structure-function similarities and differences among their luciferase enzymes. Amino acids shared between species’ sequences are likely to serve an important role in the catalysis of this reaction and identifying them will lead to ...
... comparisons among firefly species can be used to determine important structure-function similarities and differences among their luciferase enzymes. Amino acids shared between species’ sequences are likely to serve an important role in the catalysis of this reaction and identifying them will lead to ...
Affinity Chromatography
... associated with conventional cellulosic matrices available at that time. Agarose is a linear polysaccharide consisting of alternating 1,3-linked -D-galactose and 1,4-linked 3,6-anhydro--L-galactose units (13). Third, the report exploited the activation of Sepharose by treatment with cyanogen bromi ...
... associated with conventional cellulosic matrices available at that time. Agarose is a linear polysaccharide consisting of alternating 1,3-linked -D-galactose and 1,4-linked 3,6-anhydro--L-galactose units (13). Third, the report exploited the activation of Sepharose by treatment with cyanogen bromi ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
... Screen genome for relationships with previously studied viruses [10] From virus’ DNA they compute the proteins it produces [1] Compute proteins’ three-dimensional structure and thereby obtain clues about their functions n Screen for similar proteins sequences with known structure [15] n If any are f ...
... Screen genome for relationships with previously studied viruses [10] From virus’ DNA they compute the proteins it produces [1] Compute proteins’ three-dimensional structure and thereby obtain clues about their functions n Screen for similar proteins sequences with known structure [15] n If any are f ...
Interpreting the Genetic Code
... Changes in stop codon meaning must have occurred after meanings were “frozen” in other organisms, alternatively organisms that exhibit them must have evolved from organisms that never shared the universal genetic code All changes in stop codons must include three changes: 1 Replacement of former sto ...
... Changes in stop codon meaning must have occurred after meanings were “frozen” in other organisms, alternatively organisms that exhibit them must have evolved from organisms that never shared the universal genetic code All changes in stop codons must include three changes: 1 Replacement of former sto ...
Glutathione Breakthrough: Advancement in
... Glutathione (GSH) is one of the most critical molecules of the entire body. GSH plays an important role in antioxidant defense, nutrient metabolism and is key in a vast number of cellular processes including gene expression, DNA and protein synthesis, cell proliferation and apoptosis, signal transdu ...
... Glutathione (GSH) is one of the most critical molecules of the entire body. GSH plays an important role in antioxidant defense, nutrient metabolism and is key in a vast number of cellular processes including gene expression, DNA and protein synthesis, cell proliferation and apoptosis, signal transdu ...
RuBisCO in Non-Photosynthetic Alga Euglena longa: Divergent
... from E. gracilis (143.170 kb) [15,16]. The sets of genes encoding proteins involved in transcription and translation are nearly identical, except for the rps18 gene missing from E. longa. In both species the genome harbors a region comprising three tandemly arrayed operons, each including the 16S, 2 ...
... from E. gracilis (143.170 kb) [15,16]. The sets of genes encoding proteins involved in transcription and translation are nearly identical, except for the rps18 gene missing from E. longa. In both species the genome harbors a region comprising three tandemly arrayed operons, each including the 16S, 2 ...
Globins in Nonvertebrate Species: Dispersal by Horizontal Gene
... John Czeluzniak, 5 Morris Goodman, 5 Mark Blaxter,ll and Serge Vinogradofl *Department of Biochemistry, University of Antwerp; TDepartment of Morphology, Systematics and Ecology, University of Ghent; *Department of Radiology and Enrico Fermi Institute, University of Chicago; SDepartment of Anatomy, ...
... John Czeluzniak, 5 Morris Goodman, 5 Mark Blaxter,ll and Serge Vinogradofl *Department of Biochemistry, University of Antwerp; TDepartment of Morphology, Systematics and Ecology, University of Ghent; *Department of Radiology and Enrico Fermi Institute, University of Chicago; SDepartment of Anatomy, ...
Document
... Asparagine: Deamidation | Glycosylation Aspartate: Succinimide formation Glutamate: Carboxylation ...
... Asparagine: Deamidation | Glycosylation Aspartate: Succinimide formation Glutamate: Carboxylation ...
Building proteins from C, coordinates using the dihedral probability
... certainly improve the results for these residues. RMSDs for the different backbone and side-chain dihedrals are shown in Table 4. Although the side-chain dihedrals are not as well modeled as the backbone, the results are encouraging with respect to other methods. As discussed below, the DPG Protein ...
... certainly improve the results for these residues. RMSDs for the different backbone and side-chain dihedrals are shown in Table 4. Although the side-chain dihedrals are not as well modeled as the backbone, the results are encouraging with respect to other methods. As discussed below, the DPG Protein ...
Lecture 1 – Classification - LCQB
... There exist no remain of primitive proteins. All information about protein structures is derived from the proteins of present-day organisms, and the current protein universe represents a time-sliced view of all proteins at their various stages of evolution. ...
... There exist no remain of primitive proteins. All information about protein structures is derived from the proteins of present-day organisms, and the current protein universe represents a time-sliced view of all proteins at their various stages of evolution. ...
BIO450 Primer Design Tutorial
... The annealing temperature for a PCR reaction is based on the melting temperature (Tm) of the primers bound to the templates. The Tm is the temperature at which a population of double stranded DNA molecules has half of the molecules in a single stranded state and half in the double stranded state. At ...
... The annealing temperature for a PCR reaction is based on the melting temperature (Tm) of the primers bound to the templates. The Tm is the temperature at which a population of double stranded DNA molecules has half of the molecules in a single stranded state and half in the double stranded state. At ...
Lecture 1 – Classification - LCQB
... There exist no remain of primitive proteins. All information about protein structures is derived from the proteins of present-day organisms, and the current protein universe represents a time-sliced view of all proteins at their various stages of evolution. ...
... There exist no remain of primitive proteins. All information about protein structures is derived from the proteins of present-day organisms, and the current protein universe represents a time-sliced view of all proteins at their various stages of evolution. ...
E-mail: - HAL
... the target protein does not have obvious homologues in the Protein Data Bank (PDB).3 Fold recognition methods, which attempt to detect a structural template when sequence similarity is not immediately recognizable, can be an alternative,4 albeit only partial, because the structural database, while l ...
... the target protein does not have obvious homologues in the Protein Data Bank (PDB).3 Fold recognition methods, which attempt to detect a structural template when sequence similarity is not immediately recognizable, can be an alternative,4 albeit only partial, because the structural database, while l ...
A standard nomenclature for von Willebrand factor gene mutations
... Pseudogene sequence A partial unprocessed VWF pseudogene is located on chromosome 22 at 22p12-pter. The sequence for the majority of the pseudogene, including its 5’ boundary, was published by Mancuso et al in 1991 (8). The sequence spans 21-29 kb and is equivalent to exons 23-34 of the VWF gene (in ...
... Pseudogene sequence A partial unprocessed VWF pseudogene is located on chromosome 22 at 22p12-pter. The sequence for the majority of the pseudogene, including its 5’ boundary, was published by Mancuso et al in 1991 (8). The sequence spans 21-29 kb and is equivalent to exons 23-34 of the VWF gene (in ...
PhytoREF: a reference database of the plastidial 16S
... techniques. For most taxa, taxonomic identification is greatly hindered by their minute size (as small as 0.8 µm for the prasinophyte Ostreococcus; Courties et al. 1994; Vaulot et al. 2008), lack of distinctive morphological features, and fragility when classical fixatives are used (Vaulot et al. 19 ...
... techniques. For most taxa, taxonomic identification is greatly hindered by their minute size (as small as 0.8 µm for the prasinophyte Ostreococcus; Courties et al. 1994; Vaulot et al. 2008), lack of distinctive morphological features, and fragility when classical fixatives are used (Vaulot et al. 19 ...
Cloning and Molecular Analysis of the Plasma ... Paramecium tetraurelia
... constitute a multigene family which currently consists of four known genes [ 10, 11, 361 and additional isoforms which are the result of alternative RNA splicing [lo, 11, 361. Members of the gene family have been cloned from a variety of tissues of higher organisms including: human erythrocytes, ter ...
... constitute a multigene family which currently consists of four known genes [ 10, 11, 361 and additional isoforms which are the result of alternative RNA splicing [lo, 11, 361. Members of the gene family have been cloned from a variety of tissues of higher organisms including: human erythrocytes, ter ...
Supporting document 1 Safety assessment
... DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred during the transformation event. The csr1-2 gene expression cassette in CV127 is identical in sequence to the transforming plasmid DNA except for three point mutations, one o ...
... DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred during the transformation event. The csr1-2 gene expression cassette in CV127 is identical in sequence to the transforming plasmid DNA except for three point mutations, one o ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.