Membrane Topology of Cytochrome P450 2B4 in
... with a single transmembrane α-helical anchor, exhibits identical biphasic kinetics when injected beneath phosphatidylcholine monolayers. The soluble cytochromes, P450cam and Mb, and the truncated proteins, P450 2B4 Δ2–27 and Δb5, in contrast, induce only slow-phase pressure changes. The lack of a fa ...
... with a single transmembrane α-helical anchor, exhibits identical biphasic kinetics when injected beneath phosphatidylcholine monolayers. The soluble cytochromes, P450cam and Mb, and the truncated proteins, P450 2B4 Δ2–27 and Δb5, in contrast, induce only slow-phase pressure changes. The lack of a fa ...
Structural And Functional Studies Of Nicotinamide Adenine
... Invasive infections caused by Streptococcus pyogenes, also known as Group A Strep (GAS), results in approximately 600,000 deaths annually. With evidence of antibiotic-resistant strains of this bacterium on the rise, there is a need for the identification of new drug targets to control these infectio ...
... Invasive infections caused by Streptococcus pyogenes, also known as Group A Strep (GAS), results in approximately 600,000 deaths annually. With evidence of antibiotic-resistant strains of this bacterium on the rise, there is a need for the identification of new drug targets to control these infectio ...
Odyssey of Agrobacterium T-DNA.
... T-DNA complex by the VirD4 protein, which is located in the inner membrane and is absolutely required for transfer. The VirB/VirD4 apparatus delivers the T-DNA–VirD2 complex and VirE2 protein to the plant cell cytoplasm. Once they are within the plant cell, a final T-DNA complex is formed by coating ...
... T-DNA complex by the VirD4 protein, which is located in the inner membrane and is absolutely required for transfer. The VirB/VirD4 apparatus delivers the T-DNA–VirD2 complex and VirE2 protein to the plant cell cytoplasm. Once they are within the plant cell, a final T-DNA complex is formed by coating ...
sequence-structure relationship - HAL
... description, the local structure descriptions was extended to longer fragments, using an unsupervised clustering method called "Hybrid Protein Model" (HPM, (de Brevern and Hazout, 2001; de Brevern and Hazout, 2003)). The HPM topology is a neural network represented by a ring of neurons. Each neuron ...
... description, the local structure descriptions was extended to longer fragments, using an unsupervised clustering method called "Hybrid Protein Model" (HPM, (de Brevern and Hazout, 2001; de Brevern and Hazout, 2003)). The HPM topology is a neural network represented by a ring of neurons. Each neuron ...
soy protein concentrate - US Soybean Export Council
... Information in this technical bulletin was provided by The Solae Company, St. Louis, Missouri, USA. The U.S. Soybean Export Council sincerely thanks The Solae Company for its cooperation and assistance in producing this technical bulletin. For more information about The Solae Company, please see the ...
... Information in this technical bulletin was provided by The Solae Company, St. Louis, Missouri, USA. The U.S. Soybean Export Council sincerely thanks The Solae Company for its cooperation and assistance in producing this technical bulletin. For more information about The Solae Company, please see the ...
Review Non-ribosomal peptide synthetases: Identifying the cryptic
... in an uncharacterized A-domain, then the probability of their substrates being the same is high (Stachelhaus et al. 1999). This comparative analysis is used by many NRPS prediction tools like the SBS-PKS, PKS/NRPS and NP.searcher (table 1) to predict the approximate product chemistry of novel NRPS c ...
... in an uncharacterized A-domain, then the probability of their substrates being the same is high (Stachelhaus et al. 1999). This comparative analysis is used by many NRPS prediction tools like the SBS-PKS, PKS/NRPS and NP.searcher (table 1) to predict the approximate product chemistry of novel NRPS c ...
SUC1 and SUC2: two sucrose transporters from Arabidopsis
... PMAI gene containing a unique EcoRI cloning site (Figure 3). This vector allows direct insertion of SUC1 and SUC2 cDNAs. The PMA 1 promoter was chosen because it is very active in glucose-grown yeast cells, and the H÷ATPase is one of the most prominent proteins of the plasma membrane. Figure 4 shows ...
... PMAI gene containing a unique EcoRI cloning site (Figure 3). This vector allows direct insertion of SUC1 and SUC2 cDNAs. The PMA 1 promoter was chosen because it is very active in glucose-grown yeast cells, and the H÷ATPase is one of the most prominent proteins of the plasma membrane. Figure 4 shows ...
Substrate specificity of protein kinase A in reaction with
... eukaryote signaling and according to the human genome analysis 518 putative protein kinase genes were identified (Manning et al, 2002). This makes them the largest enzyme family in eukaryotic cells. Despite the large amount of sequence data for protein kinases, only a small fraction of these enzymes ...
... eukaryote signaling and according to the human genome analysis 518 putative protein kinase genes were identified (Manning et al, 2002). This makes them the largest enzyme family in eukaryotic cells. Despite the large amount of sequence data for protein kinases, only a small fraction of these enzymes ...
Hitting the Target: Emerging Technologies in the Search for Kinase
... conductor. Aside from containing sequences necessary for three-dimensional folding into domains, many proteins have evolved motifs that allow posttranslational modifications that modulate function. By far the largest group of enzymes that catalyze regulatory posttranslational modifications is the fa ...
... conductor. Aside from containing sequences necessary for three-dimensional folding into domains, many proteins have evolved motifs that allow posttranslational modifications that modulate function. By far the largest group of enzymes that catalyze regulatory posttranslational modifications is the fa ...
using the crispr/cas9 gene editing tool to develop a cure for
... quickly improved the use of CRISPR, although there are some variations in how it is used. The most important component is the CRISPR RNA molecule, which includes two types of RNA: programmable guide RNA and tracer RNA. These two parts typically function as two separate molecules, but Jennifer Doudna ...
... quickly improved the use of CRISPR, although there are some variations in how it is used. The most important component is the CRISPR RNA molecule, which includes two types of RNA: programmable guide RNA and tracer RNA. These two parts typically function as two separate molecules, but Jennifer Doudna ...
Transcription Factor EB Controls Metabolic Flexibility during
... TFEB in skeletal muscle increased the expression of mitochondrial enzymes. Subunits of the four respiratory chain complexes and the ATP synthase, as well as genes encoding electron transport and tricarboxylic acid cycle proteins, were induced by TFEB overexpression and were reduced by TFEB deletion ...
... TFEB in skeletal muscle increased the expression of mitochondrial enzymes. Subunits of the four respiratory chain complexes and the ATP synthase, as well as genes encoding electron transport and tricarboxylic acid cycle proteins, were induced by TFEB overexpression and were reduced by TFEB deletion ...
... leading to the emergence of molecular nutrition as a new cornerstone in nutrition research. Molecular nutrition investigates roles for nutrients at the molecular level, such as signal transduction, gene expression and covalent modifications of proteins. Research findings that have been generated by ...
Structure based hypothesis of a mitochondrial
... and predicted for mtRF1a (Additional file 5: Figure S2B). Taken together, the results from our modeling experiments show that mtRF1 contains a blocked stop codon binding site as a result of several conserved changes when compared to its closest paralog mtRF1a. Several polar amino acids involved in n ...
... and predicted for mtRF1a (Additional file 5: Figure S2B). Taken together, the results from our modeling experiments show that mtRF1 contains a blocked stop codon binding site as a result of several conserved changes when compared to its closest paralog mtRF1a. Several polar amino acids involved in n ...
Journal of Biological Chemistry
... upon amino acid incorporation into protein of the liver ribosome system (Table II) just as effectively as it does the decrease in hepatic ATP concentration induced by the same analogue (3). These results tend to implicate cellular BTP deficiency as being important in the inhibition of protein synthe ...
... upon amino acid incorporation into protein of the liver ribosome system (Table II) just as effectively as it does the decrease in hepatic ATP concentration induced by the same analogue (3). These results tend to implicate cellular BTP deficiency as being important in the inhibition of protein synthe ...
Proteins - The Open University
... Defence Antibodies (immunoglobulins) are proteins that recognise specific targets (usually proteins themselves). This facility is critical for an immune response. Transport Proteins are key molecules in the transport of substances both within a cell and to and from the cell. Storage A number of prot ...
... Defence Antibodies (immunoglobulins) are proteins that recognise specific targets (usually proteins themselves). This facility is critical for an immune response. Transport Proteins are key molecules in the transport of substances both within a cell and to and from the cell. Storage A number of prot ...
Coordination of the maize transcriptome by a conserved circadian
... with "cellular amino acid and derivative metabolic process" (GO: 0006519), which showed overrepresentation of genes with peak levels at ZT20 (Table 1, Figure 2). These results indicate maize protein synthesis may also have a pre-dawn phase. Interestingly, Arabidopsis genes associated with protein sy ...
... with "cellular amino acid and derivative metabolic process" (GO: 0006519), which showed overrepresentation of genes with peak levels at ZT20 (Table 1, Figure 2). These results indicate maize protein synthesis may also have a pre-dawn phase. Interestingly, Arabidopsis genes associated with protein sy ...
Protein Purification by Inverse Transition Cycling
... apparently remains sufficiently hydrated to prevent its denaturation. Thus, an ELP tag genetically incorporated into a protein of interest provides a “molecular handle” to manipulate the protein, which allows easy and rapid purification, concentration, and buffer exchange. Potential applications of ...
... apparently remains sufficiently hydrated to prevent its denaturation. Thus, an ELP tag genetically incorporated into a protein of interest provides a “molecular handle” to manipulate the protein, which allows easy and rapid purification, concentration, and buffer exchange. Potential applications of ...
promoting training adaptations through nutritional
... AMPK is a critical signalling protein involved in the regulation of multiple metabolic and growth responses in skeletal muscle in response to exercise. This ‘fuel sensing’ enzyme is involved in acute exercise-induced events and also plays an obligatory role in adapting skeletal muscles to repeated b ...
... AMPK is a critical signalling protein involved in the regulation of multiple metabolic and growth responses in skeletal muscle in response to exercise. This ‘fuel sensing’ enzyme is involved in acute exercise-induced events and also plays an obligatory role in adapting skeletal muscles to repeated b ...
pdf
... purpose because of its low frequency in E. coli and the observation that natural amber suppressors do not affect growth rates significantly. The other two nonsense codons, TAA and TGA, and several frameshift codons also have been used successfully (2, 3, 7, 14, 15). In the case of the tRNAPro/ProRS p ...
... purpose because of its low frequency in E. coli and the observation that natural amber suppressors do not affect growth rates significantly. The other two nonsense codons, TAA and TGA, and several frameshift codons also have been used successfully (2, 3, 7, 14, 15). In the case of the tRNAPro/ProRS p ...
PDF
... expressions in leaves. We used qRT-PCR with gene-specific primers to quantify the expression levels of NtMTHFR1, CYP82E4 and CYP82E5 in four OX (OX-4, -5, -8 and -10) lines with low NCRs (around 1.7 to 2%) and four KD (KD-4, -6, -7 and -9) lines with high NCRs (around 7 to 9%) compared to the vector ...
... expressions in leaves. We used qRT-PCR with gene-specific primers to quantify the expression levels of NtMTHFR1, CYP82E4 and CYP82E5 in four OX (OX-4, -5, -8 and -10) lines with low NCRs (around 1.7 to 2%) and four KD (KD-4, -6, -7 and -9) lines with high NCRs (around 7 to 9%) compared to the vector ...
Accurate Prediction of Contact Numbers for Multi
... where n is the length of the protein chain in the case of computing the monomeric contact number or the total number of residues in the protein for computing the oligomeric contact number. All nonprotein molecules were removed before computing the contact numbers. Nonprotein molecules such as coenzy ...
... where n is the length of the protein chain in the case of computing the monomeric contact number or the total number of residues in the protein for computing the oligomeric contact number. All nonprotein molecules were removed before computing the contact numbers. Nonprotein molecules such as coenzy ...
Steven`s project - The University of Texas at Dallas
... have yet to be fully understood. The folded state of a protein is the state in which it finally gains its functionality within the organism. As a result, it has become very important to try to predict these folding patterns for various reasons. Also, it has been found that the final shape of a folde ...
... have yet to be fully understood. The folded state of a protein is the state in which it finally gains its functionality within the organism. As a result, it has become very important to try to predict these folding patterns for various reasons. Also, it has been found that the final shape of a folde ...
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES
... Metalloproteins represent a large portion of the total proteome. When bound to a protein a metal ion influences both protein stability and function through structural, catalytic or regulatory roles. Discovery of a metal ion cofactor presents new insight into both the structural and functional aspect ...
... Metalloproteins represent a large portion of the total proteome. When bound to a protein a metal ion influences both protein stability and function through structural, catalytic or regulatory roles. Discovery of a metal ion cofactor presents new insight into both the structural and functional aspect ...
Sample Chapter 5: Amino Acids, Peptides, and Proteins
... linear sequence specified by the base sequence of the DNA-generated mRNA for that protein. The ability of each type of the tens of thousands of different proteins to perform its functions is specified by its unique amino acid sequence. During synthesis each polypeptide molecule bends in three-dimens ...
... linear sequence specified by the base sequence of the DNA-generated mRNA for that protein. The ability of each type of the tens of thousands of different proteins to perform its functions is specified by its unique amino acid sequence. During synthesis each polypeptide molecule bends in three-dimens ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.