New lysosomal acid lipase gene mutants explain the phenotype of
... tion at the 21 position of exon 8 donor site (the 59 splice site of the corresponding intron) that results in exon skipping and the subsequent loss of 24 amino acids from the protein is the most frequent defect observed in patients with CESD (4, 7–9). Skipping of the same exon has also been observed ...
... tion at the 21 position of exon 8 donor site (the 59 splice site of the corresponding intron) that results in exon skipping and the subsequent loss of 24 amino acids from the protein is the most frequent defect observed in patients with CESD (4, 7–9). Skipping of the same exon has also been observed ...
- BioMed Central
... in the generation of a massive amount of expression data. One of the greatest challenge we are faced with is to then analyse the data as a whole and extract the meaningful relationships among specific genes. Standard methods such as SAM [6] or machine learning algorithms [7] are able to detect patte ...
... in the generation of a massive amount of expression data. One of the greatest challenge we are faced with is to then analyse the data as a whole and extract the meaningful relationships among specific genes. Standard methods such as SAM [6] or machine learning algorithms [7] are able to detect patte ...
Transcriptome analysis of the two unrelated fungal
... Background: Cephalosporins and penicillins are the most frequently used β-lactam antibiotics for the treatment of human infections worldwide. The main industrial producers of these antibiotics are Acremonium chrysogenum and Penicillium chrysogenum, two taxonomically unrelated fungi. Both were subjec ...
... Background: Cephalosporins and penicillins are the most frequently used β-lactam antibiotics for the treatment of human infections worldwide. The main industrial producers of these antibiotics are Acremonium chrysogenum and Penicillium chrysogenum, two taxonomically unrelated fungi. Both were subjec ...
Pan-cancer analysis of the metabolic reaction network
... Figure 2 – Classification of reactions based on their presence across 917 cancer genome-scale metabolic models. (A) Depiction of reaction classification. Core reactions are present in virtually all models, either by means of the same gene-reaction association (pan) or via different isoenzymes (iso). ...
... Figure 2 – Classification of reactions based on their presence across 917 cancer genome-scale metabolic models. (A) Depiction of reaction classification. Core reactions are present in virtually all models, either by means of the same gene-reaction association (pan) or via different isoenzymes (iso). ...
Relationship Between the Occurrence of Cysteine in Proteins and
... Lacey 1978). Therefore, the genetic code is designed to minimize the chance that a single nucleotide base change will result in replacement with a physicochemically different amino acid. The result of nontargeted introduction of a cysteine must have been that many cysteines were not at ‘‘optimal pla ...
... Lacey 1978). Therefore, the genetic code is designed to minimize the chance that a single nucleotide base change will result in replacement with a physicochemically different amino acid. The result of nontargeted introduction of a cysteine must have been that many cysteines were not at ‘‘optimal pla ...
NOVEL SPERM-SPECIFIC GLYCOLYTIC ISOZYMES GENERATED
... spermatogenesis indicates that glycolysis is essential for sperm motility and male fertility. This pathway is compartmentalized in the principal piece of the sperm flagellum, where several spermatogenic cell-specific isozymes are bound to a cytoskeletal structure known as the fibrous sheath. Fructos ...
... spermatogenesis indicates that glycolysis is essential for sperm motility and male fertility. This pathway is compartmentalized in the principal piece of the sperm flagellum, where several spermatogenic cell-specific isozymes are bound to a cytoskeletal structure known as the fibrous sheath. Fructos ...
Production of exopolysaccharide from mycelial culture of Grifola
... and antitumor agents for clinical use [13–15]. Apart from these applications, polysaccharides have been used as a substance that enhances the skins natural ability to heal and protect itself against infection [2]. Recently, several polysaccharides have been used as alternative ingredients for enhan ...
... and antitumor agents for clinical use [13–15]. Apart from these applications, polysaccharides have been used as a substance that enhances the skins natural ability to heal and protect itself against infection [2]. Recently, several polysaccharides have been used as alternative ingredients for enhan ...
A method for measuring the non-random bias of a codon usage table
... attempt to distinguish between coding and non-coding regions. ...
... attempt to distinguish between coding and non-coding regions. ...
cloning of gs1 gene encodes glutamine synthetase 1 and
... isolated cDNA of GS1 with other sequences on Genbank (Accession number X69822.1 and X74429.1), the similarities are both higher than 99%. This result indicates a high sequence conservation of GS1 gene. The gene was then ligated into vector pBI121 to construct transformation vector pBI121 – GS1 which ...
... isolated cDNA of GS1 with other sequences on Genbank (Accession number X69822.1 and X74429.1), the similarities are both higher than 99%. This result indicates a high sequence conservation of GS1 gene. The gene was then ligated into vector pBI121 to construct transformation vector pBI121 – GS1 which ...
STING Millennium: a web-based suite of programs
... because any point on the structure/surface might be painted (colored) only once, following the color code designed for given amino acid characteristics. Amino acid sequence on the other hand, can be mapped by number of parameters, organized and displayed in underlying rows. Appropriate visualization ...
... because any point on the structure/surface might be painted (colored) only once, following the color code designed for given amino acid characteristics. Amino acid sequence on the other hand, can be mapped by number of parameters, organized and displayed in underlying rows. Appropriate visualization ...
Origins and Early Evolution of the tRNA Molecule
... [22]. Although these classes may have two distinct evolutionary origins, the tertiary structures of aaRSs revealed that a member of each of the two classes can be docked simultaneously Class I synthetases possess active sites with the Rossmann fold motif and consensus sequences onto the opposite sid ...
... [22]. Although these classes may have two distinct evolutionary origins, the tertiary structures of aaRSs revealed that a member of each of the two classes can be docked simultaneously Class I synthetases possess active sites with the Rossmann fold motif and consensus sequences onto the opposite sid ...
Question bank in Biology class XII
... 2. Give scientific name of two helminthes that are pathogene to man . 3. What is incubatiuon period ? how long it is for AIDS virus ? 4. Name and explain the two types of immune responses in humans . 5. Name the two special types of lymphocytes in humans . How do they differ in their roles in immune ...
... 2. Give scientific name of two helminthes that are pathogene to man . 3. What is incubatiuon period ? how long it is for AIDS virus ? 4. Name and explain the two types of immune responses in humans . 5. Name the two special types of lymphocytes in humans . How do they differ in their roles in immune ...
Responses of Growing Broilers to Diets with Increased Sulfur Amino
... investigations. In this context, sulfur amino acids (SAA, methionine and cysteine) play a very important role in growing broilers because they are essential for optimum muscle accretion and feather synthesis as well as for some biochemical processes (i.e., as methyl-group donators). However, SAA are ...
... investigations. In this context, sulfur amino acids (SAA, methionine and cysteine) play a very important role in growing broilers because they are essential for optimum muscle accretion and feather synthesis as well as for some biochemical processes (i.e., as methyl-group donators). However, SAA are ...
Lactococcus lactis as expression host for the biosynthetic
... strains to stimulate the incorporation of Trp analogues [18]. This approach was explored for L. lactis PA1001 in CDM. A concentration of 0.2 mg/ml of IAA completely inhibited the growth of L. lactis PA1001 (Figure 3A). However, addition of Trp did not elevate the IAA-inhibitory effect, suggesting th ...
... strains to stimulate the incorporation of Trp analogues [18]. This approach was explored for L. lactis PA1001 in CDM. A concentration of 0.2 mg/ml of IAA completely inhibited the growth of L. lactis PA1001 (Figure 3A). However, addition of Trp did not elevate the IAA-inhibitory effect, suggesting th ...
Arctigenin Efficiently Enhanced Sedentary Mice Treadmill
... 29]. Since AMPK activation by actual exercise or pharmacological treatment could lead to up-regulation of PGC-1a gene expression [30,31], we investigated the potential effects of arctigenin on PGC1a mRNA level in both H9C2 and C2C12 cells. Compared with DMSO-treated group, arctigenin incubation coul ...
... 29]. Since AMPK activation by actual exercise or pharmacological treatment could lead to up-regulation of PGC-1a gene expression [30,31], we investigated the potential effects of arctigenin on PGC1a mRNA level in both H9C2 and C2C12 cells. Compared with DMSO-treated group, arctigenin incubation coul ...
Role of Dietary Protein in Post-Exercise Muscle Reconditioning
... capacity. The latter has renewed the interest among athletes, coaches, and scientists in the role of the diet and nutritional modulation in optimizing training efficiency. This chapter will provide an overview on the impact of dietary protein administration following exercise on the skeletal muscle ...
... capacity. The latter has renewed the interest among athletes, coaches, and scientists in the role of the diet and nutritional modulation in optimizing training efficiency. This chapter will provide an overview on the impact of dietary protein administration following exercise on the skeletal muscle ...
Chapter 18
... However, LDL-mediated endocytosis is not a factor for the development of atherosclerosis: Two reasons, still atherosclerosis 1. Intracellular regulation: LDLR gene activity is cholesterol-dependent; High intracellular cholesterol →insig/SCAP/SREBP pathway ↓→ LDLR gene regulation ↓→ low LDLR gene exp ...
... However, LDL-mediated endocytosis is not a factor for the development of atherosclerosis: Two reasons, still atherosclerosis 1. Intracellular regulation: LDLR gene activity is cholesterol-dependent; High intracellular cholesterol →insig/SCAP/SREBP pathway ↓→ LDLR gene regulation ↓→ low LDLR gene exp ...
Thermodynamic analysis of DNA binding by a Bacillus single
... Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations ≤100 μg/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high ...
... Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations ≤100 μg/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high ...
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
... instructions. Sequenase was obtained from Cambridge Bioscience. Enzyme I was the generous gift of Professor E. B. Waygood (University of Saskatchewan, Saskatoon, Canada). Construction of episomal gene library. F32-episomal DNA (McFall, 1967)was purified by the method described by Dardel et al. (1984 ...
... instructions. Sequenase was obtained from Cambridge Bioscience. Enzyme I was the generous gift of Professor E. B. Waygood (University of Saskatchewan, Saskatoon, Canada). Construction of episomal gene library. F32-episomal DNA (McFall, 1967)was purified by the method described by Dardel et al. (1984 ...
Specific Isotopic Labeling of Methyl Groups has Extended the
... and other ILV methyl groups to be observed over long distances (e.g. up to 8 Å)23. This labeling strategy is often combined with uniform 15N and 2H labeling of aliphatic sites other than ILV methyl groups labeling and allows long-range amide-methyl and methyl-methyl NOEs to be observed using appropr ...
... and other ILV methyl groups to be observed over long distances (e.g. up to 8 Å)23. This labeling strategy is often combined with uniform 15N and 2H labeling of aliphatic sites other than ILV methyl groups labeling and allows long-range amide-methyl and methyl-methyl NOEs to be observed using appropr ...
HIGHLY VISCOUS DOUGH FORMING PROPERTIES OF MARAMA PROTEIN
... defined as the ratio of G" over G', reflects the balance between the viscous and the elastic character of a viscoelastic material (Mezger, 2006). As small dynamic deformation analysis is non-destructive, it can provide some information on the types of molecular bonding that may be responsible for th ...
... defined as the ratio of G" over G', reflects the balance between the viscous and the elastic character of a viscoelastic material (Mezger, 2006). As small dynamic deformation analysis is non-destructive, it can provide some information on the types of molecular bonding that may be responsible for th ...
Statistical potential-based amino acid similarity
... residues surrounding the positions. Therefore, two residues sitting at equivalent positions in two proteins are expected to have a similar residue contact preference. For any two given amino acids, the similarity of the residue contact preference can be computed by comparing the residue contact pote ...
... residues surrounding the positions. Therefore, two residues sitting at equivalent positions in two proteins are expected to have a similar residue contact preference. For any two given amino acids, the similarity of the residue contact preference can be computed by comparing the residue contact pote ...
A new polymerase chain reaction/restriction fragment length
... using AluI identified fragments of 10, 27, 38, 54, 106, and 135 bp for VK210 and 10, 38, and 673 bp for VK247, whereas P. vivax-like showed an unique fragment of 10 and 726 bp. In respect to RFLP, using DpnI, we observed fragments with sizes of 27, 42, 54, 81, 108, and 301 bp (VK247), whereas P. viv ...
... using AluI identified fragments of 10, 27, 38, 54, 106, and 135 bp for VK210 and 10, 38, and 673 bp for VK247, whereas P. vivax-like showed an unique fragment of 10 and 726 bp. In respect to RFLP, using DpnI, we observed fragments with sizes of 27, 42, 54, 81, 108, and 301 bp (VK247), whereas P. viv ...
Lecture 3 Isoelectric Focusing
... It will become -ve (pH>pI and migrate to the anode (+electrode attracts -ion)) As it passes through the gel, eg when it reaches pH 9 region, it becomes less -vely charged (pH not so much >pI) (+electrode does not attract protein so much – protein slows down) When reaches the pH 8 region, protein cha ...
... It will become -ve (pH>pI and migrate to the anode (+electrode attracts -ion)) As it passes through the gel, eg when it reaches pH 9 region, it becomes less -vely charged (pH not so much >pI) (+electrode does not attract protein so much – protein slows down) When reaches the pH 8 region, protein cha ...
ppt - Bio 5068
... Analysis of the Quality of NMR Protein Structures With A Structure Calculated From Your NMR Data, How Do You Determine the Accuracy and Quality of the Structure? • Consistency with Known Protein Structural Parameters bond lengths, bond angles, dihedral angles, VDW interactions, etc all the struct ...
... Analysis of the Quality of NMR Protein Structures With A Structure Calculated From Your NMR Data, How Do You Determine the Accuracy and Quality of the Structure? • Consistency with Known Protein Structural Parameters bond lengths, bond angles, dihedral angles, VDW interactions, etc all the struct ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.