Translational Repression of C. elegans p53 by GLD
... 2003; Vassilev et al., 2004). Such a therapeutic approach highlights the need to uncover additional pathways and mechanisms that negatively regulate p53 levels or activity. Most studies on p53 signaling have been conducted in cell culture-based systems, and their translation into mouse models is oft ...
... 2003; Vassilev et al., 2004). Such a therapeutic approach highlights the need to uncover additional pathways and mechanisms that negatively regulate p53 levels or activity. Most studies on p53 signaling have been conducted in cell culture-based systems, and their translation into mouse models is oft ...
A novel C. elegans zinc finger transcription factor, lsy
... ASEL and ASER develop from a common ground state to express a number of features that are specific for ASEL versus ASER (Fig. 1A). These left/right asymmetric features include the expression of several putative chemoreceptors of the GCY family. For example, in adult animals, the gcy-7 gene is exclus ...
... ASEL and ASER develop from a common ground state to express a number of features that are specific for ASEL versus ASER (Fig. 1A). These left/right asymmetric features include the expression of several putative chemoreceptors of the GCY family. For example, in adult animals, the gcy-7 gene is exclus ...
Transcriptome analysis of Drosophila CNS midline cells reveals
... expressed in a variety of midline cell types, including motoneurons, GABAergic interneurons, and midline glia. These data revealed previously unknown functional differences among the highly-related iVUM neurons. There also exist segmental differences in expression for the same neuronal sub-type. Sim ...
... expressed in a variety of midline cell types, including motoneurons, GABAergic interneurons, and midline glia. These data revealed previously unknown functional differences among the highly-related iVUM neurons. There also exist segmental differences in expression for the same neuronal sub-type. Sim ...
First report of a tetracycline-inducible gene
... been re-engineered for use in mollicutes. Among these, the availability of an inducible promoter system, in particular, is critical for studying gene function. In fact, the control of gene expression has a wide range of applications such as production of conditional knockout mutants (Kamionka et al. ...
... been re-engineered for use in mollicutes. Among these, the availability of an inducible promoter system, in particular, is critical for studying gene function. In fact, the control of gene expression has a wide range of applications such as production of conditional knockout mutants (Kamionka et al. ...
Ectopic induction and reorganization of Wnt-1
... 3380 L. Bally-Cuif and M. Wassef These studies on mutant mice (McMahon et al., 1992) have also identified En-1 as a gene whose early maintenance of expression in mesencephalon was dependent on Wnt-1; but later-occurring regulatory interactions in met-mesencephalon could not be studied since the who ...
... 3380 L. Bally-Cuif and M. Wassef These studies on mutant mice (McMahon et al., 1992) have also identified En-1 as a gene whose early maintenance of expression in mesencephalon was dependent on Wnt-1; but later-occurring regulatory interactions in met-mesencephalon could not be studied since the who ...
Linköping University Post Print Segment-specific Neuronal Sub-type Specification by the Integration of
... divisions, ‘‘budding’’ off secondary progenitor cells denoted ...
... divisions, ‘‘budding’’ off secondary progenitor cells denoted ...
Transgenic approaches for optogenetics Tim Murphy UBC.
... Cre recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the si ...
... Cre recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the si ...
Two Waves of Nuclear Factor κB Recruitment to Target Promoters
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
Class17 1-31 Win16 Cell Cycle Notes
... Draw E2F, and include binding sites for other molecules. -Where are they in relation to each other? Does E2F binds to Rb or promoters more tightly? Why is E2F called a ‘transcription factor’? ...
... Draw E2F, and include binding sites for other molecules. -Where are they in relation to each other? Does E2F binds to Rb or promoters more tightly? Why is E2F called a ‘transcription factor’? ...
Promega Notes 49: Firefly Luciferase Engineered for Improved
... iv) Glycosylation sites. Native luciferase expressed in the peroxisomes or the cytosol normally does not contain any post-translational modifications. However, gene fusions may be made of luc+ (or luc+NF) which are intended to direct a hybrid protein into the endoplasmic reticulum or Golgi apparatus ...
... iv) Glycosylation sites. Native luciferase expressed in the peroxisomes or the cytosol normally does not contain any post-translational modifications. However, gene fusions may be made of luc+ (or luc+NF) which are intended to direct a hybrid protein into the endoplasmic reticulum or Golgi apparatus ...
Supporting Information Parfenov et al. 10.1073/pnas.1416074111
... P values (adjusted by the Benjamini–Hochberg procedure) less than 0.05 were considered as significantly differentially methylated or expressed. The sites and corresponding statistics for all CpG loci and genes can be found in Dataset S1, Tables S3 and S4. Additionally, we carried out an unsupervised ...
... P values (adjusted by the Benjamini–Hochberg procedure) less than 0.05 were considered as significantly differentially methylated or expressed. The sites and corresponding statistics for all CpG loci and genes can be found in Dataset S1, Tables S3 and S4. Additionally, we carried out an unsupervised ...
Mutations affecting craniofacial development in zebrafish
... In homozygous bielak (bilm429) embryos, the ethmoid plate, as well as Meckel’s cartilage, quadrate, ceratohyal and hyosymplectic are smaller (Fig. 2G,O). The ceratohyal points caudally. While the first and the fifth ceratobranchials are visible in all mutant embryos, the second ceratobranchial is ra ...
... In homozygous bielak (bilm429) embryos, the ethmoid plate, as well as Meckel’s cartilage, quadrate, ceratohyal and hyosymplectic are smaller (Fig. 2G,O). The ceratohyal points caudally. While the first and the fifth ceratobranchials are visible in all mutant embryos, the second ceratobranchial is ra ...
Gene Section USF1 (upstream transcription factor 1) Atlas of Genetics and Cytogenetics
... distribution of E-box like elements in the genome. Whole-genome ChIP analysis in HepG2 cells identified 2518 USF1 binding sites in chromatin context, of which 41 % were located within 1 kb of a transcription start site (Rade-Iglesias et al., 2008). USF1 binding signals strongly correlate with target ...
... distribution of E-box like elements in the genome. Whole-genome ChIP analysis in HepG2 cells identified 2518 USF1 binding sites in chromatin context, of which 41 % were located within 1 kb of a transcription start site (Rade-Iglesias et al., 2008). USF1 binding signals strongly correlate with target ...
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... expression. We also demonstrate that following initiation of expression, CEH-36 autoregulates to maintain its own expression via direct binding to a cognate site in its upstream regulatory sequences. Moreover, CEH-36 directly regulates the expression of terminal differentiation genes in the AWC neur ...
... expression. We also demonstrate that following initiation of expression, CEH-36 autoregulates to maintain its own expression via direct binding to a cognate site in its upstream regulatory sequences. Moreover, CEH-36 directly regulates the expression of terminal differentiation genes in the AWC neur ...
Embryonic Development following Somatic Cell
... [FC] > 5, FPKM > 5) that can be classified into five groups by unsupervised hierarchical cluster analysis (Figure 1D). Of these 3,775 differentially expressed genes, 1,549 were activated in both SCNT and IVF embryos (groups 1 and 2). Gene ontology (GO) analysis revealed that these genes were signif ...
... [FC] > 5, FPKM > 5) that can be classified into five groups by unsupervised hierarchical cluster analysis (Figure 1D). Of these 3,775 differentially expressed genes, 1,549 were activated in both SCNT and IVF embryos (groups 1 and 2). Gene ontology (GO) analysis revealed that these genes were signif ...
Cytokinin and auxin have a crucial role in the control of this balance.
... for specifying the embryonic root stemcell niche during Arabidopsis embryogenesis [55]. The Arabidopsis embryonic root stem cell niche is initiated by the specification of a single cell, the hypophysis. By combining a new visualization tool for cytokinin two-component-output sensor and inducible gen ...
... for specifying the embryonic root stemcell niche during Arabidopsis embryogenesis [55]. The Arabidopsis embryonic root stem cell niche is initiated by the specification of a single cell, the hypophysis. By combining a new visualization tool for cytokinin two-component-output sensor and inducible gen ...
"Silencing of Gene Expression in Cultured Cells Using Small
... initiated a new wave of reverse genetics in mammalian systems. The siRNA-mediated silencing provides fast, target-specific down-regulation of gene expression. The extent to which one can successfully carry out the silencing for determination of gene function depends on the potency of siRNAs, transfe ...
... initiated a new wave of reverse genetics in mammalian systems. The siRNA-mediated silencing provides fast, target-specific down-regulation of gene expression. The extent to which one can successfully carry out the silencing for determination of gene function depends on the potency of siRNAs, transfe ...
Bonus, a Drosophila TIF1 homologue, is a chromatin
... Bonus, a Drosophila TIF1 homolog, is a nuclear receptor cofactor that is required for viability, molting, and numerous morphological events. Here we establish a role for Bonus in the modulation of chromatin structure. We show that weak loss-of-function alleles of bonus have a more deleterious effect ...
... Bonus, a Drosophila TIF1 homolog, is a nuclear receptor cofactor that is required for viability, molting, and numerous morphological events. Here we establish a role for Bonus in the modulation of chromatin structure. We show that weak loss-of-function alleles of bonus have a more deleterious effect ...
Diversity of DNA methyltransferases that recognize asymmetric
... palindromic DNA sequences and add a methyl group to the target base (either adenine or cytosine) on both strands. However, there are a number of MTases that recognize asymmetric target sequences and differ in their subunit organization. In a bacterial cell, after each round of replication, the subst ...
... palindromic DNA sequences and add a methyl group to the target base (either adenine or cytosine) on both strands. However, there are a number of MTases that recognize asymmetric target sequences and differ in their subunit organization. In a bacterial cell, after each round of replication, the subst ...
Rapid generation of nested chromosomal
... cloning of genes (2). All of these uses have been beautifully exemplified by a set of nested deletions over the albino locus on mouse chromosome 7, which has been used to define essential genes, conduct mutagenesis, and map and clone genes (2–4). Complementation analysis was used to first assign fun ...
... cloning of genes (2). All of these uses have been beautifully exemplified by a set of nested deletions over the albino locus on mouse chromosome 7, which has been used to define essential genes, conduct mutagenesis, and map and clone genes (2–4). Complementation analysis was used to first assign fun ...
Polymorphic Variation in the Structure of Aerial
... Further investigations of the phenotype of aerial sclerotia produced by different isolates from the wild have shown that two distinct structures can be found. The structure which has previously been described as being produced by strain H1 will be called the H-type. The second polymorphic form was f ...
... Further investigations of the phenotype of aerial sclerotia produced by different isolates from the wild have shown that two distinct structures can be found. The structure which has previously been described as being produced by strain H1 will be called the H-type. The second polymorphic form was f ...
Discovery and characterization of chromatin states for Please share
... 89% of each state was within 2kb of a RefSeq transcription start site (TSS), compared with 2.7% genome-wide (P<10−200, for all states). These states accounted for 59% of all RefSeq TSS while covering only 1.3% of genome. These states all had in common a high frequency of H3K4me3, but differed in ter ...
... 89% of each state was within 2kb of a RefSeq transcription start site (TSS), compared with 2.7% genome-wide (P<10−200, for all states). These states accounted for 59% of all RefSeq TSS while covering only 1.3% of genome. These states all had in common a high frequency of H3K4me3, but differed in ter ...
PDF - Oxford Academic - Oxford University Press
... Background and Aims The arrangement of flowers in inflorescence shoots of Arabidopsis thaliana represents a regular spiral Fibonacci phyllotaxis. However, in the cuc2 cuc3 double mutant, flower pedicels are fused to the inflorescence stem, and phyllotaxis is aberrant in the mature shoot regions. T ...
... Background and Aims The arrangement of flowers in inflorescence shoots of Arabidopsis thaliana represents a regular spiral Fibonacci phyllotaxis. However, in the cuc2 cuc3 double mutant, flower pedicels are fused to the inflorescence stem, and phyllotaxis is aberrant in the mature shoot regions. T ...
Root Hairs - Biological Sciences
... embryonic root development to establish the proper pattern of gene activities that ultimately leads to appropriate postembryonic cell type differentiation. To determine whether positional information is also provided to epidermal cells post-embryonically, two sorts of experiments have been conducted ...
... embryonic root development to establish the proper pattern of gene activities that ultimately leads to appropriate postembryonic cell type differentiation. To determine whether positional information is also provided to epidermal cells post-embryonically, two sorts of experiments have been conducted ...
Glutathione S-transferase copy number variation alters lung gene expression M.W. Butler*
... RNA per 106 cells. In order to visualise and quantify the degree of RNA integrity, an aliquot of each sample of RNA was analysed with the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The concentration was determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologi ...
... RNA per 106 cells. In order to visualise and quantify the degree of RNA integrity, an aliquot of each sample of RNA was analysed with the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The concentration was determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologi ...