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... 20 M for each assay (supplementary material Table S2). The cDNA of each individual blastomere was subjected to sequence-specific amplification prior to analysis with the Universal Probe Library (UPL) for gene expression assays (Roche) in 48⫻48 Dynamic Arrays on a BioMark ...
... 20 M for each assay (supplementary material Table S2). The cDNA of each individual blastomere was subjected to sequence-specific amplification prior to analysis with the Universal Probe Library (UPL) for gene expression assays (Roche) in 48⫻48 Dynamic Arrays on a BioMark ...
Developmental fate and lineage commitment of singled mouse
... 20 M for each assay (supplementary material Table S2). The cDNA of each individual blastomere was subjected to sequence-specific amplification prior to analysis with the Universal Probe Library (UPL) for gene expression assays (Roche) in 48⫻48 Dynamic Arrays on a BioMark ...
... 20 M for each assay (supplementary material Table S2). The cDNA of each individual blastomere was subjected to sequence-specific amplification prior to analysis with the Universal Probe Library (UPL) for gene expression assays (Roche) in 48⫻48 Dynamic Arrays on a BioMark ...
Involvement of Sox1, 2 and 3 in the early and subsequent molecular
... 2522 Y. Kamachi and others hours). Because of this early response, we have anticipated that the mechanism of δ1-crystallin gene activation is highly relevant to the lens induction process, reflecting the second state being met within the ectoderm. We have found that δ1-crystallin gene is regulated ...
... 2522 Y. Kamachi and others hours). Because of this early response, we have anticipated that the mechanism of δ1-crystallin gene activation is highly relevant to the lens induction process, reflecting the second state being met within the ectoderm. We have found that δ1-crystallin gene is regulated ...
Making Headway: The Roles of Hox Genes and Neural
... Genetic analyses in several vertebrate species have shown that Hox genes are essential for specifying segmental identity in the developing hindbrain. The Hox gene family comprises a set of developmentally regulated transcription factors that are characterized by a conserved DNA binding motif called ...
... Genetic analyses in several vertebrate species have shown that Hox genes are essential for specifying segmental identity in the developing hindbrain. The Hox gene family comprises a set of developmentally regulated transcription factors that are characterized by a conserved DNA binding motif called ...
paramecium tetraurelia
... Genetic analysis was carried out according to the methods developed and reviewed by SONNEBORN (1970). The two main features of the analysis are: (1) i n a cross A x B the two exconjugants of each pair represent respectively the two reciprocal crosses OB x $ A and $ B x OA; (2) : the F, generation is ...
... Genetic analysis was carried out according to the methods developed and reviewed by SONNEBORN (1970). The two main features of the analysis are: (1) i n a cross A x B the two exconjugants of each pair represent respectively the two reciprocal crosses OB x $ A and $ B x OA; (2) : the F, generation is ...
Bittner GMDS - Buffalo Ontology Site
... no is-a relations linking modules each module a true classificatory tree ifomis.org ...
... no is-a relations linking modules each module a true classificatory tree ifomis.org ...
MEF2 activity during embryogenesis - Development
... of transgene integration. Consistent with previous results, the hsp-lacZ transgene lacking MEF2 sites was not expressed at detectable levels in embryos, neonates or adult mice (Kothary et al., 1989; data not shown). Moreover, two independent F0 founder transgenic mice that carried the hsp-lacZ repor ...
... of transgene integration. Consistent with previous results, the hsp-lacZ transgene lacking MEF2 sites was not expressed at detectable levels in embryos, neonates or adult mice (Kothary et al., 1989; data not shown). Moreover, two independent F0 founder transgenic mice that carried the hsp-lacZ repor ...
Embryo forming cells in carrot suspension cultures
... Somatic cells of many plant species can be cultured in vitro and induced to form embryos that areabletodevelop intomature plants.Thisprocess,termed somatic embryogenesis, was originally described in carrot (Daucuscarota L.).Somatic embryosdevelopthrough thesamecharacteristicmorphological stages,i.e. ...
... Somatic cells of many plant species can be cultured in vitro and induced to form embryos that areabletodevelop intomature plants.Thisprocess,termed somatic embryogenesis, was originally described in carrot (Daucuscarota L.).Somatic embryosdevelopthrough thesamecharacteristicmorphological stages,i.e. ...
The Role of H2A.Z in Chromosome Segregation in
... independently linked to kinetochore components and are important for chromosome stability. It has also been found that they all have synthetic interactions with both kinetochore and mitotic spindle mutants (Krogan et al., 2004). Additionally, there is an increase in chromosome loss rates in htz1∆ mu ...
... independently linked to kinetochore components and are important for chromosome stability. It has also been found that they all have synthetic interactions with both kinetochore and mitotic spindle mutants (Krogan et al., 2004). Additionally, there is an increase in chromosome loss rates in htz1∆ mu ...
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... and polarized light sensors (Hardie, 1985). According to their differentiated function, R1-6 innervate the first OL neuropil, which is the lamina, whereas R7 and R8 axons run across the lamina to innervate the neuropil beneath, which is the medulla. Next, the lobula and lobula plate (together called ...
... and polarized light sensors (Hardie, 1985). According to their differentiated function, R1-6 innervate the first OL neuropil, which is the lamina, whereas R7 and R8 axons run across the lamina to innervate the neuropil beneath, which is the medulla. Next, the lobula and lobula plate (together called ...
Schwann Cell-Specific Ablation of Laminin 1 Causes Apoptosis and
... axonal sorting. A, PCR analysis of genomic DNA from various tissues of wild-type, homozygous fLAM ␥1 mice (f/f; control) and P0 /Cre:fLAM ␥1 mice (f/f, P0-Cre; mutant). The primers used amplified the wild-type (1.3 kb), unrecombined (3.2 kb), and recombined (2.3 kb) fLAM ␥1 alleles. B, Transverse se ...
... axonal sorting. A, PCR analysis of genomic DNA from various tissues of wild-type, homozygous fLAM ␥1 mice (f/f; control) and P0 /Cre:fLAM ␥1 mice (f/f, P0-Cre; mutant). The primers used amplified the wild-type (1.3 kb), unrecombined (3.2 kb), and recombined (2.3 kb) fLAM ␥1 alleles. B, Transverse se ...
Apical-basal pattern formation in the Arabidopsis
... development. Consistent with this early effect, gnom appears to be epistatic to monopteros in doubly mutant embryos, suggesting that, without prior gnom activity, the monopteros gene cannot promote root and hypocotyl development. On the other hand, when root formation was induced in bisected seedlin ...
... development. Consistent with this early effect, gnom appears to be epistatic to monopteros in doubly mutant embryos, suggesting that, without prior gnom activity, the monopteros gene cannot promote root and hypocotyl development. On the other hand, when root formation was induced in bisected seedlin ...
Dynamic modification of the ETS transcription factor PEA3 by
... the mutually exclusive nature of these modifications, both contribute to the trans-activation capacity of PEA3, implying that a dynamic series of modification events occurs during the activation process. INTRODUCTION Transcription factor function is often controlled by post-translational modification ...
... the mutually exclusive nature of these modifications, both contribute to the trans-activation capacity of PEA3, implying that a dynamic series of modification events occurs during the activation process. INTRODUCTION Transcription factor function is often controlled by post-translational modification ...
Robust gene silencing mediated by antisense small RNAs in the
... Division of Infectious Diseases, Department of Internal Medicine, Stanford University School of Medicine, Stanford, California 94305-5107, USA and 2Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305-5107, USA ...
... Division of Infectious Diseases, Department of Internal Medicine, Stanford University School of Medicine, Stanford, California 94305-5107, USA and 2Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305-5107, USA ...
Valpromide Inhibits Lytic Cycle Reactivation of Epstein
... The luciferase activities were normalized to total protein levels. The data are average results for at least three separate transfections. Un, untreated control. ...
... The luciferase activities were normalized to total protein levels. The data are average results for at least three separate transfections. Un, untreated control. ...
Targeting gene expression to cones with human cone opsin
... Figure 4 Fluorescence images showing targeted green fluorescent protein (GFP) gene expression in cones. Refer to Table 1 for specific details. (a–c) 3LCR-PR0.5-GFP (dog M571, left eye, 4 weeks post-subretinal vector administration). (a) Native GFP expression visualized by excitation with blue light. ...
... Figure 4 Fluorescence images showing targeted green fluorescent protein (GFP) gene expression in cones. Refer to Table 1 for specific details. (a–c) 3LCR-PR0.5-GFP (dog M571, left eye, 4 weeks post-subretinal vector administration). (a) Native GFP expression visualized by excitation with blue light. ...
Gene Section MYB (v-myb myeloblastosis viral oncogene homolog (avian))
... binds to its cognate binding site (MYB binding site MBS; consensus A/C A A C G/T G) on target genes and regulates their expression. MYB is essential for the establishment of definitive haemopoiesis; as such, myb/mice die of anoxia by embryonic day 15. Conditional knockout mice have shown that myb is ...
... binds to its cognate binding site (MYB binding site MBS; consensus A/C A A C G/T G) on target genes and regulates their expression. MYB is essential for the establishment of definitive haemopoiesis; as such, myb/mice die of anoxia by embryonic day 15. Conditional knockout mice have shown that myb is ...
Auxin represses stomatal development in dark
... in aux/iaa mutants do not form prematurely but post-embryonically, similar to the wild type. We subsequently investigated how the increased SI in darkgrown axr3-1 reflects the total, absolute number of stomata and pavement cells per cotyledon. Fig. 2B shows that at 2 dpg the total number of epiderma ...
... in aux/iaa mutants do not form prematurely but post-embryonically, similar to the wild type. We subsequently investigated how the increased SI in darkgrown axr3-1 reflects the total, absolute number of stomata and pavement cells per cotyledon. Fig. 2B shows that at 2 dpg the total number of epiderma ...
BMC Developmental Biology
... this by overexpressing a functional copy of the Dll1 gene throughout the paraxial mesoderm, in particular in anterior somite compartments, during somitogenesis in transgenic mice. Results: Epithelial somites were generated normally and appeared histologically normal in embryos of two independent Dll ...
... this by overexpressing a functional copy of the Dll1 gene throughout the paraxial mesoderm, in particular in anterior somite compartments, during somitogenesis in transgenic mice. Results: Epithelial somites were generated normally and appeared histologically normal in embryos of two independent Dll ...
Gene Section USP1 (ubiquitin specific peptidase 1) Atlas of Genetics and Cytogenetics
... In addition to its DNA damage-related functions, USP1 has also been reported to deubiquitinate and stabilize three members of the family of inhibitors of DNA binding (ID) proteins (ID1, ID2 and ID3), and thus contributing to preserve the undifferentiated state of osteosarcoma cells (Williams et al., ...
... In addition to its DNA damage-related functions, USP1 has also been reported to deubiquitinate and stabilize three members of the family of inhibitors of DNA binding (ID) proteins (ID1, ID2 and ID3), and thus contributing to preserve the undifferentiated state of osteosarcoma cells (Williams et al., ...
X chromosome inactivation- Review
... Xist RNA may cause replication origins to fire late, resulting in heterochromatin formation modulate histone acetetylation – The Drosophila mof gene is required for dosage compensation and is an acetyltransferase ...
... Xist RNA may cause replication origins to fire late, resulting in heterochromatin formation modulate histone acetetylation – The Drosophila mof gene is required for dosage compensation and is an acetyltransferase ...
Translational control of regA, a key gene controlling
... plasmids integrate into the Volvox genome at random locations, frequently in the form of multiple tandem repeats (Babinger et al., 2001). The Nit+ transformants that were recovered in the experiments reported below exhibited 70 to 80% co-transformation of the unselected marker (in terms of integrati ...
... plasmids integrate into the Volvox genome at random locations, frequently in the form of multiple tandem repeats (Babinger et al., 2001). The Nit+ transformants that were recovered in the experiments reported below exhibited 70 to 80% co-transformation of the unselected marker (in terms of integrati ...
Expression and function of decapentaplegic and thick veins during
... and tkv mutations that result in strong modifications of the vein pattern (data not shown), suggesting that the effect of these mutations on vein differentiation occur after the veins have been specified in the imaginal disc. In dpps combinations (dpps4/dpps8) there is a good correlation between the ...
... and tkv mutations that result in strong modifications of the vein pattern (data not shown), suggesting that the effect of these mutations on vein differentiation occur after the veins have been specified in the imaginal disc. In dpps combinations (dpps4/dpps8) there is a good correlation between the ...
Basement membranes - Development
... which assemble into three distinct protomers, α1.α1.α2(IV), α3.α4.α5(IV) and α5.α5.α6(IV) (Boutaud et al., 2000; Hudson et al., 2003). In addition to the ubiquitous α1/α2 network, two independent networks, α3.α4.α5(IV) and a combined aggregate of α1.α1.α2/α5.α5.α6(IV) molecules, have been described ...
... which assemble into three distinct protomers, α1.α1.α2(IV), α3.α4.α5(IV) and α5.α5.α6(IV) (Boutaud et al., 2000; Hudson et al., 2003). In addition to the ubiquitous α1/α2 network, two independent networks, α3.α4.α5(IV) and a combined aggregate of α1.α1.α2/α5.α5.α6(IV) molecules, have been described ...
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... CGG repeats (28,29). However, the length of CGG repeats on a particular allele in a differentiated cell derived from these carriers is generally mitotically stable although recent studies show that fully expanded FMR1 CGG repeats exhibit a lengthand differentiation-dependent instability in cell hybr ...
... CGG repeats (28,29). However, the length of CGG repeats on a particular allele in a differentiated cell derived from these carriers is generally mitotically stable although recent studies show that fully expanded FMR1 CGG repeats exhibit a lengthand differentiation-dependent instability in cell hybr ...