lecture - Berkeley MCB
... What democracy, I mean, gene regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have seq ...
... What democracy, I mean, gene regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have seq ...
Prep1.1 has essential genetic functions in hindbrain development
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
Prep1.1 has essential genetic functions in hindbrain development
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
Forkhead Transcription Factors: Key Players in Development and
... up in nucleosomes than to naked DNA (Cirillo and Zaret, 1999). However, binding by FoxA1 does not compact chromatin, as linker histones do, but is instead correlated with an active chromatin structure in the albumin enhancer (Cirillo et al., 1998). Furthermore, binding by FoxA proteins to nucleosome ...
... up in nucleosomes than to naked DNA (Cirillo and Zaret, 1999). However, binding by FoxA1 does not compact chromatin, as linker histones do, but is instead correlated with an active chromatin structure in the albumin enhancer (Cirillo et al., 1998). Furthermore, binding by FoxA proteins to nucleosome ...
Julio`s MCB - Digital Access to Scholarship at Harvard
... the expression of Mfrn1. Mutagenesis of individual GATA-1 binding cis elements (GBE) demonstrated that at least two of the three GBE within this CRM are functionally required for GATA-mediated transcription of Mfrn1. Furthermore, ChIP assays demonstrate switching from GATA-2 to GATA-1 at these eleme ...
... the expression of Mfrn1. Mutagenesis of individual GATA-1 binding cis elements (GBE) demonstrated that at least two of the three GBE within this CRM are functionally required for GATA-mediated transcription of Mfrn1. Furthermore, ChIP assays demonstrate switching from GATA-2 to GATA-1 at these eleme ...
Full Text - Global Science Books
... rust responses ranging from 7 to 9. Of 92 families tested, 84 were resistant and eight were susceptible at the Karalee site. This segregation conformed to three to four gene model (Table 1). In contrast, an increased number of families showed susceptible responses at the Lansdowne site in 2004. Chi- ...
... rust responses ranging from 7 to 9. Of 92 families tested, 84 were resistant and eight were susceptible at the Karalee site. This segregation conformed to three to four gene model (Table 1). In contrast, an increased number of families showed susceptible responses at the Lansdowne site in 2004. Chi- ...
Gene Section RAC2 (ras-related C3 botulinum toxin substrate 2
... fusion protein (Thomas et al., 2007). These genetic data were further substantiated experimentally by use of NSC23766, small molecule antagonist of Rac activation, to validate biochemically and functionally Rac as a molecular target in both a relevant animal model and in primary human CML cells in v ...
... fusion protein (Thomas et al., 2007). These genetic data were further substantiated experimentally by use of NSC23766, small molecule antagonist of Rac activation, to validate biochemically and functionally Rac as a molecular target in both a relevant animal model and in primary human CML cells in v ...
Morphology of nuclear transcription | SpringerLink
... unique conformation in the genome. Individual genes are arranged in a repetitive head-to-tail orientation. The transcribed genes (transcription units; TU) are separated by intergenic spacer sequences (IGS). Several tens of such repeats form a nucleolar organizer region (NOR) which, in humans, lies e ...
... unique conformation in the genome. Individual genes are arranged in a repetitive head-to-tail orientation. The transcribed genes (transcription units; TU) are separated by intergenic spacer sequences (IGS). Several tens of such repeats form a nucleolar organizer region (NOR) which, in humans, lies e ...
FEMS Microbiology Letters
... G, similar to that in the comA null mutant (data not shown), indicating that the unphosphorylated form of ComA does not appear to have a role in the regulation of srfA. All of these results indicate that the majority of srfA expression during growth in DSM-G, in particular at late-growth phase, does ...
... G, similar to that in the comA null mutant (data not shown), indicating that the unphosphorylated form of ComA does not appear to have a role in the regulation of srfA. All of these results indicate that the majority of srfA expression during growth in DSM-G, in particular at late-growth phase, does ...
Efficient Delivery of Dharmacon SMARTpool® siRNA
... nucleofection® procedure in the absence of siRNA. The analysis of mocktreated cells will indicate whether the transfection process results in cytotoxicity or other non-specific effects. Identifying optimal effective siRNA reagent concentrations When performing siRNA-mediated knockdown experiments it ...
... nucleofection® procedure in the absence of siRNA. The analysis of mocktreated cells will indicate whether the transfection process results in cytotoxicity or other non-specific effects. Identifying optimal effective siRNA reagent concentrations When performing siRNA-mediated knockdown experiments it ...
1 Supplemental Table 1. FACS-isolated, SSEA-4
... -induces cartilage and bone formation, -based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. -the protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclea ...
... -induces cartilage and bone formation, -based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. -the protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclea ...
PDF
... secretory region of the main segment and therefore encompass both SCs and bar-shaped cells. tio but not tsh is expressed in the distalmost tubule cell known as the tip cell (data not shown). These dynamic patterns of tsh and tio expression are in line with previous observations, where mutually exclu ...
... secretory region of the main segment and therefore encompass both SCs and bar-shaped cells. tio but not tsh is expressed in the distalmost tubule cell known as the tip cell (data not shown). These dynamic patterns of tsh and tio expression are in line with previous observations, where mutually exclu ...
Gsp1 Triggers the Sexual Developmental Program
... characterize mutants with defective uniparental inheritance (Sager and Ramanis, 1974; Sager et al., 1981). Gillham et al. (1987) isolated three mutations, mat-3-1, 3-2, and 3-3, that are linked to the mt+ locus, all showing increased transmission of the mt2 cpDNA. However, the mat-3 cells are unusua ...
... characterize mutants with defective uniparental inheritance (Sager and Ramanis, 1974; Sager et al., 1981). Gillham et al. (1987) isolated three mutations, mat-3-1, 3-2, and 3-3, that are linked to the mt+ locus, all showing increased transmission of the mt2 cpDNA. However, the mat-3 cells are unusua ...
Tissue Polarity Genes of Drosophila Regulate the Subcellular
... any subdivision. As shown in Fig. 5, these subdivisions have arrows showing their approximate orientation. Blank subregions have hairs with a distal polarity (that is, a wild-type polarity). We call any region where neighboring hairs share a common abnormal (i.e., not distal) polarity abnormal patch ...
... any subdivision. As shown in Fig. 5, these subdivisions have arrows showing their approximate orientation. Blank subregions have hairs with a distal polarity (that is, a wild-type polarity). We call any region where neighboring hairs share a common abnormal (i.e., not distal) polarity abnormal patch ...
Ribosomal RNA Secondary Structure
... pairing and nonpairing bases are 1,082 (54.4%) and 907 (45.6%)) respectively (table 1). These proportions are not significantly different [x2 = 0.03, degrees of freedom (df) = 1, P > 0.81 from the proportions predicted by the Xenopus model of secondary structure for the entire 28s gene [ 2,244 (54.6 ...
... pairing and nonpairing bases are 1,082 (54.4%) and 907 (45.6%)) respectively (table 1). These proportions are not significantly different [x2 = 0.03, degrees of freedom (df) = 1, P > 0.81 from the proportions predicted by the Xenopus model of secondary structure for the entire 28s gene [ 2,244 (54.6 ...
Isolation of COV1, a gene involved in the regulation of vascular
... unconnected to, the vascular bundles, which exhibit a more normal pattern of development (Fig. 2). It can also be seen that additional differentiation occurs directly adjacent to the developing vascular bundles (Fig. 2). It is unclear at present whether this is actually lateral expansion of the esta ...
... unconnected to, the vascular bundles, which exhibit a more normal pattern of development (Fig. 2). It can also be seen that additional differentiation occurs directly adjacent to the developing vascular bundles (Fig. 2). It is unclear at present whether this is actually lateral expansion of the esta ...
Gsp1 Triggers the Sexual Developmental Program
... which are contributed by the mt+ and mt2 gametes, respectively. GSP1 was identified as a gene expressed specifically in mt+ gametes (Kurvari et al., 1998). When GSP1 is ectopically expressed in mt2 gametes, the cells transcribe genes that are normally transcribed only after zygote formation (Zhao et a ...
... which are contributed by the mt+ and mt2 gametes, respectively. GSP1 was identified as a gene expressed specifically in mt+ gametes (Kurvari et al., 1998). When GSP1 is ectopically expressed in mt2 gametes, the cells transcribe genes that are normally transcribed only after zygote formation (Zhao et a ...
DnaJ-related protein essential for placentation - Development
... showed no abnormal phenotypes in other trophoblast cell types or in the embryo proper. This study indicates a previously unsuspected role for chaperone proteins in placental development and represents the first genetic analysis of DnaJ-related protein function in higher eukaryotes. Based on a survey ...
... showed no abnormal phenotypes in other trophoblast cell types or in the embryo proper. This study indicates a previously unsuspected role for chaperone proteins in placental development and represents the first genetic analysis of DnaJ-related protein function in higher eukaryotes. Based on a survey ...
Cell division: The renaissance of the centriole
... duplication. Centrioles are the only organelles besides chromosomes that duplicate precisely once per cell division. Exactly one new, ‘daughter’ centriole forms adjacent to and at right angles with each pre-existing centriole. While this suggests that the pre-existing centriole acts as a template fo ...
... duplication. Centrioles are the only organelles besides chromosomes that duplicate precisely once per cell division. Exactly one new, ‘daughter’ centriole forms adjacent to and at right angles with each pre-existing centriole. While this suggests that the pre-existing centriole acts as a template fo ...
Targeted gene expression as a means of altering cell fates and
... GAL4 vector, we modified the enhancer detection lacZ vector, plwB (Wilson et al., 1989). To remove the NotI site in the vector, plwB was digested with NotI and the 5′ overhanging ends were filled using T4 polymerase. The resultant blunt ends were then ligated to make plasmid p41-4. To remove the P-t ...
... GAL4 vector, we modified the enhancer detection lacZ vector, plwB (Wilson et al., 1989). To remove the NotI site in the vector, plwB was digested with NotI and the 5′ overhanging ends were filled using T4 polymerase. The resultant blunt ends were then ligated to make plasmid p41-4. To remove the P-t ...
Figures and figure supplements
... shown. Underlying colors indicate the similarity between the different datasets. Note that H3K36me3 was flagged for low signal to noise, potentially explaining the somewhat high correlation with H3K27me3 (see Figure 1—source data 4). However, this does not affect the conclusions of the paper in any ...
... shown. Underlying colors indicate the similarity between the different datasets. Note that H3K36me3 was flagged for low signal to noise, potentially explaining the somewhat high correlation with H3K27me3 (see Figure 1—source data 4). However, this does not affect the conclusions of the paper in any ...
PowerPoint 프레젠테이션
... synthetic siRNA, vector based siRNA is the same as DNA, and it is very stable and can be easily tranfected into cell using routine DNA transfection reagents, such as Lipofectamine. 3.Stable cell line can be established: vector based siRNA allows you to obtain a stable cell line, and observe long-ter ...
... synthetic siRNA, vector based siRNA is the same as DNA, and it is very stable and can be easily tranfected into cell using routine DNA transfection reagents, such as Lipofectamine. 3.Stable cell line can be established: vector based siRNA allows you to obtain a stable cell line, and observe long-ter ...
Foxf1 controls mesoderm differentiation
... VEGF receptors (Flk1 and Flt1) in the mesoderm which promote vascular morphogenesis and differentiation of endothelial and hematopoietic cells (Fong et al., 1995; Fong et al., 1999; Shalaby et al., 1995; Yamaguchi et al., 1993). Angiogenesis and differentiation of other vascular cell types, such as ...
... VEGF receptors (Flk1 and Flt1) in the mesoderm which promote vascular morphogenesis and differentiation of endothelial and hematopoietic cells (Fong et al., 1995; Fong et al., 1999; Shalaby et al., 1995; Yamaguchi et al., 1993). Angiogenesis and differentiation of other vascular cell types, such as ...
Additional data file 9
... to be expressed in the endocrine system. We discovered nine TFs, CG11762, CG8145, CG11723, CG9876, knot (kn), diminutive (dm), pou domain motif 3 (pdm3), Mes2 and ventral veins lacking (vv)l with endocrine expression. The four CGs have not been previously characterized and are uniquely expressed in ...
... to be expressed in the endocrine system. We discovered nine TFs, CG11762, CG8145, CG11723, CG9876, knot (kn), diminutive (dm), pou domain motif 3 (pdm3), Mes2 and ventral veins lacking (vv)l with endocrine expression. The four CGs have not been previously characterized and are uniquely expressed in ...