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9 Genomics and Beyond
9 Genomics and Beyond

... (1) Genome is cut into small, overlapping fragments with a restriction enzyme, and each piece is cloned, forming a DNA library. (2) The DNA fragments must overlap other fragments, so the restriction enzyme is not allowed to cut at every possible restriction site. (3) Computers assemble the fragments ...
Ch. 10: Presentation Slides
Ch. 10: Presentation Slides

... acids that they encode • Main features of the genetic code were proved in genetic experiments carried out by F.Crick and collaborators: • Translation starts from a fixed point • There is a single reading frame maintained throughout the process of translation • Each codon consists of three nucleotide ...
Chemistry of Life
Chemistry of Life

... is putting two molecules (in this case simple sugars) together and forming a complex molecule (in this case sugar). • Monosaccharides are put together forming ...
g.ML-6 DNA Replication1
g.ML-6 DNA Replication1

... initiation sites. Fusion studies provide important insights into the activation process. When an S phase cell is fused to a cell in G1, the nucleus of the G1 cell begins DNA synthesis. Thus, the pre- initiation complex that is bound to the DNA in G1 is competent to polymerize and only needs the act ...
Mutation Activity
Mutation Activity

... - To demonstrate how the three types of mutations occur (insertion, deletion, and substitution). - To demonstrate the effects of the three types of mutations on the amino acid chain produced by a DNA strand. Background: The genetic makeup of all known living things is carried in a genetic material k ...
Bio 313 worksheet 14 - Iowa State University
Bio 313 worksheet 14 - Iowa State University

... steps with the first step being the DNA molecule. Second step being the primary transcript ...
Brooker Chapter 10
Brooker Chapter 10

... • Regions that are always heterochromatic • Permanently inactive with regard to transcription ...
Chapter 20 Notes: DNA Technology
Chapter 20 Notes: DNA Technology

... 2) Isolate plasmid from bacterial cell; 3) cut both DNA samples with the same restriction enzyme to open up bacterial plasmid & create sticky ends on both ...
Genetic Technology
Genetic Technology

... genotype individual would you need to perform a test cross with to determine your genotype?  Draw a punnett square and determine what ratio of genotypes your offspring would have.  If you did not have any blonde hair children, what does that mean your genotype must be? ...
Document
Document

... B) (4pts) What are the two most likely splice patterns you would observe in the mRNA (use exon numbers for answers)? ...
Tech Notes Use of Plasmid-Safe™ to Prevent Cloning Artifacts Due
Tech Notes Use of Plasmid-Safe™ to Prevent Cloning Artifacts Due

... Use of Plasmid-Safe™ to Prevent Cloning Artifacts Due to Bacterial Chromosomal DNA Ribozymes are catalytic RNAs that Even following purification in CsCl/ethidium bromide gradients, plasmid and cosmid preparations may still contain contaminating bacterial chromosomal DNA. This contaminating DNA can b ...
Modern Genetics Notes
Modern Genetics Notes

... nitrogen (15N) and then moved them to a medium containing light nitrogen (“N), allowing the bacteria to replicate and divide once. The new bacterial DNA contained DNA consisting of one heavy strand and one light strand, thus proving Watson and Crick’s theory.heir now-famous model of DNA. ...
The Polymerase Chain Reaction (PCR) enables researchers to
The Polymerase Chain Reaction (PCR) enables researchers to

... not limited to just searching real DNA anymore but can search for specific DNA sequences online. However, since many of the databases and the tools to search these are freely available to anybody, you can be a DNA sleuth anytime you have an internet connection available. What can be used to search f ...
What meaning(s) do these two photos represent? (Hint* dna,rna
What meaning(s) do these two photos represent? (Hint* dna,rna

... which can make several forms of a protein.  Takes a lot of energy to make initial large molecule. Rule for gene expression, use to state until the 1990s: 1 gene = 1 protein Not true any more 1 gene = can make many proteins ...
Pyrimidines
Pyrimidines

... 3 primary RNA components: 5S, 28S, 23S  5s RNA is too small (not enough information available),  23s RNA is too large, evolving more rapidly  16s RNA is of manageable size and information content ...
Mock Exam 3 Chapters 14-18 Anthony Todd  http
Mock Exam 3 Chapters 14-18 Anthony Todd http

... b. III only c. I and II d. I and III e. I, II, and III 48. What are control elements found thousands of nucleotides upstream or downstream of a gene? a. Transcription factors b. Enhancers c. Promoters d. Activators e. Operators 49. Which of the following is not a way that genes can be regulated by t ...
ppt
ppt

... Taking a perfectly good fat and making it bad! Addition of hydrogen atoms to the acid, causing double bonds to become single ones. (unsaturated becomes saturated) LDL HDL ...
2nd YR MY Lab DNA extract_Molecular Genetics Lect 2nd yr
2nd YR MY Lab DNA extract_Molecular Genetics Lect 2nd yr

... 3. Collecting DNA The most frequently used method of concentration is ethanol precipitation. In the presence of salt and at a temperature of -20 °C or less, absolute ethanol will efficiently precipitate polymeric nucleic acids. With a concentrated solution of DNA one can use a glass rod to spool th ...
CA Update from Dr. Beever 07-26-2010
CA Update from Dr. Beever 07-26-2010

... In an effort to find the specific mutation within this chromosome segment that causes CA, numerous genes were selected for analysis based on their biological function and relationship to the pathology that had been described . In the end, DNA sequences were analyzed for more than 40 genes using DNA ...
Gene expression (central dogma)
Gene expression (central dogma)

... acts as a template for the synthesis of a matching (complementary) RNA strand by an enzyme called RNA polymerase. This RNA strand is the primary transcript. ...
Molecular Genetics - Fall River Public Schools
Molecular Genetics - Fall River Public Schools

... • tRNA transports free floating amino acids to the ribosomes based on the codons in mRNA – One side of tRNA attaches to an amino acid – On the loop opposite of the amino acid attachment site is a sequence of 3 nucleotides called anticodons – Anticodons are complimentary to and pair with the correspo ...
Chapter 13 Selective breeding is a technique of choosing specific
Chapter 13 Selective breeding is a technique of choosing specific

MCB Lecture 9 – Mitchondria – Van Oost
MCB Lecture 9 – Mitchondria – Van Oost

Learned about mutations
Learned about mutations

... instructions. If the protein doesn’t function as well as the original, the mutation would be bad. If the protein functions better than the original, the mutation would be good. If the same protein is made as the original, the mutation would have no effect. In this activity you will be using magnets ...
Ch - Humble ISD
Ch - Humble ISD

... Catabolism – chemical rxns (usually Hydrolysis reactions) Break down larger food molecules into smaller chem. units; release energy ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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