AN INTEGRATED MAP OF CATTLE CANDIDATE GENES FOR
... To facilitate the development of new genetic markers for mastitis resistance or susceptibility we used genome-wide comparative approach to review all known mastitis-associated loci. We assembled into a map 233 loci that were identified by six different study approaches (QTLs, association studies, ex ...
... To facilitate the development of new genetic markers for mastitis resistance or susceptibility we used genome-wide comparative approach to review all known mastitis-associated loci. We assembled into a map 233 loci that were identified by six different study approaches (QTLs, association studies, ex ...
CAIcal: A combined set of tools to assess codon usage adaptation
... codon positions, and the effective number of codons [17]. (ii) The CAI of a DNA sequence or group of sequences. This index measures the adaptation of the synonymous codon usage of a gene to the synonymous codon usage of up to two reference sets that can be chosen by the user. The new features incorp ...
... codon positions, and the effective number of codons [17]. (ii) The CAI of a DNA sequence or group of sequences. This index measures the adaptation of the synonymous codon usage of a gene to the synonymous codon usage of up to two reference sets that can be chosen by the user. The new features incorp ...
Word - NIEHS SNPs Program - University of Washington
... 6. Notice the SNP sites across the top of the image are listed by RS_ID. What is the RS_ID for the first nonsynonymous SNP from the left? 7. Close the VG2 image and Select Display Type windows and return to the Genome Variation Server Page. Now, change the allele frequency cutoff to 5. How many SNPs ...
... 6. Notice the SNP sites across the top of the image are listed by RS_ID. What is the RS_ID for the first nonsynonymous SNP from the left? 7. Close the VG2 image and Select Display Type windows and return to the Genome Variation Server Page. Now, change the allele frequency cutoff to 5. How many SNPs ...
Evidence, Mechanisms and Models for the Inheritance of Acquired
... We have suggested previously that epigenetic inheritance systems are important not only in ontogeny, but also in phylogeny (Jablonka & Lamb, 1989). They can lead to " L a m a r c k i a n " inheritance. Our arguments were based on the biological properties of some EISs, on evidence showing that chrom ...
... We have suggested previously that epigenetic inheritance systems are important not only in ontogeny, but also in phylogeny (Jablonka & Lamb, 1989). They can lead to " L a m a r c k i a n " inheritance. Our arguments were based on the biological properties of some EISs, on evidence showing that chrom ...
Plasmodium malariae and P. ovale genomes provide insights into
... The present study highlights features of host-specific adaptations at several levels: ape to human, primate to rodent and within human hosts. As noted in previous comparative genomics studies involving host switches in the Plasmodium genus, invasion-related genes are consistently found to be rapidly ...
... The present study highlights features of host-specific adaptations at several levels: ape to human, primate to rodent and within human hosts. As noted in previous comparative genomics studies involving host switches in the Plasmodium genus, invasion-related genes are consistently found to be rapidly ...
Chapter 12
... Chapter 12 - DNA Technology and the Human Genome How can we use bacteria to manipulate DNA and protein? Recall how a bacterium defends itself when a bacteriophage injects its DNA into a bacterium… The bacterium has enzymes called restriction enzymes that attempt to cut up the bacteriophage DNA befo ...
... Chapter 12 - DNA Technology and the Human Genome How can we use bacteria to manipulate DNA and protein? Recall how a bacterium defends itself when a bacteriophage injects its DNA into a bacterium… The bacterium has enzymes called restriction enzymes that attempt to cut up the bacteriophage DNA befo ...
Multifractal characterisation of length sequences of coding and
... in the complete genome. This integer sequence is named coding length sequence. (ii) Order all lengths of noncoding segments according to the order of noncoding segments in the complete genome. This integer sequence is named noncoding length sequence. Yu and Anh [20] proposed a time series model for ...
... in the complete genome. This integer sequence is named coding length sequence. (ii) Order all lengths of noncoding segments according to the order of noncoding segments in the complete genome. This integer sequence is named noncoding length sequence. Yu and Anh [20] proposed a time series model for ...
A rough guide to Drosophila mating schemes (version 1.2) 1
... standard approach to prove this hypothesis is to carry out "rescue experiments" by adding back a wild type copy of the gene into the mutant background, analogous to gene therapy. For this, you will need to clone the Mef2 gene and generate transgenic fly lines for the targeted expression of Mef2 (sec ...
... standard approach to prove this hypothesis is to carry out "rescue experiments" by adding back a wild type copy of the gene into the mutant background, analogous to gene therapy. For this, you will need to clone the Mef2 gene and generate transgenic fly lines for the targeted expression of Mef2 (sec ...
Efficient Screening of CRISPR/Cas9
... mutagenesis experiments, and 43 from the knock-in experiments. Individual P were crossed to e double balancer flies to uncover e and maintain lethal mutations in lbk. All F1 offspring were scored for ebony body color; not all P produced ebony offspring. We observed e F1 in 42 (62%) of the mutagenesis ...
... mutagenesis experiments, and 43 from the knock-in experiments. Individual P were crossed to e double balancer flies to uncover e and maintain lethal mutations in lbk. All F1 offspring were scored for ebony body color; not all P produced ebony offspring. We observed e F1 in 42 (62%) of the mutagenesis ...
Use of Gene Replacement Transformation to Elucidate
... also subjectto carboncatabolite repression (JOHNSTON 1987). Carbon repressionof the GAL genes is thought to operate throughseveral mechanisms such as direct action on the various GAL promoters, inhibition of activator binding and inhibition of galactose transport (JOHNSTON 1987). However, the mechan ...
... also subjectto carboncatabolite repression (JOHNSTON 1987). Carbon repressionof the GAL genes is thought to operate throughseveral mechanisms such as direct action on the various GAL promoters, inhibition of activator binding and inhibition of galactose transport (JOHNSTON 1987). However, the mechan ...
Vectors: The carriers of DNA molecules DNA vectors and their
... In general plasmid cloning vectors are designated by a lowercase ‘p’ which stands for plasmid, and some abbreviations that may be descriptive. pBR322 plasmid Plasmid pBR322 is the one of the best studied and most often used ‘”general purpose” plasmids. The BR of the pBR322 recognizes the work of the ...
... In general plasmid cloning vectors are designated by a lowercase ‘p’ which stands for plasmid, and some abbreviations that may be descriptive. pBR322 plasmid Plasmid pBR322 is the one of the best studied and most often used ‘”general purpose” plasmids. The BR of the pBR322 recognizes the work of the ...
Antisense Transcript and RNA Processing
... antisense transcript, which attenuates the degradation of the polyadenylated form. The accumulation of double-stranded RNA was confirmed by insensitivity of atpB mRNA from PSþ genome-containing cells to S1 nuclease digestion. To obtain additional evidence for antisense RNA function in chloroplasts, ...
... antisense transcript, which attenuates the degradation of the polyadenylated form. The accumulation of double-stranded RNA was confirmed by insensitivity of atpB mRNA from PSþ genome-containing cells to S1 nuclease digestion. To obtain additional evidence for antisense RNA function in chloroplasts, ...
gene duplication in the evolution of sexual dimorphism
... selected duplicates for which duplication appeared to have occurred after D. melanogaster diverged from the D. sechellia and D. simulans clade (Hahn et al. 2007). If the lineage-specific paralog families had more than two members, we included only the two duplicates at the tip of the gene tree. For ...
... selected duplicates for which duplication appeared to have occurred after D. melanogaster diverged from the D. sechellia and D. simulans clade (Hahn et al. 2007). If the lineage-specific paralog families had more than two members, we included only the two duplicates at the tip of the gene tree. For ...
The importance of alternative splicing in the drug discovery process
... information on alternative splicing has been accumulating at a rapid rate during the last four years [7–12], the core drug discovery processes entail techniques that cannot distinguish between splice variants and are therefore trapped in the old dogma of ‘one gene, one mRNA, one protein’. When the p ...
... information on alternative splicing has been accumulating at a rapid rate during the last four years [7–12], the core drug discovery processes entail techniques that cannot distinguish between splice variants and are therefore trapped in the old dogma of ‘one gene, one mRNA, one protein’. When the p ...
Nat. Struct. Biol. 8, 192-194.
... being highly promiscuous. Since this promiscuity is rarely observed, eukaryotes must have developed mechanisms to insure that genes are not activated in the wrong tissue or at the wrong time of development by enhancers from a neighboring gene. Chromatin insulators may play the role of gatekeepers th ...
... being highly promiscuous. Since this promiscuity is rarely observed, eukaryotes must have developed mechanisms to insure that genes are not activated in the wrong tissue or at the wrong time of development by enhancers from a neighboring gene. Chromatin insulators may play the role of gatekeepers th ...
Applied and Environmental Microbiology
... A PCR-based approach was developed to detect ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) form I large-subunit genes (cbbL) as a functional marker of autotrophic bacteria that fix carbon dioxide via the Calvin-Benson-Bassham cycle. We constructed two different primer sets, targeting the ...
... A PCR-based approach was developed to detect ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) form I large-subunit genes (cbbL) as a functional marker of autotrophic bacteria that fix carbon dioxide via the Calvin-Benson-Bassham cycle. We constructed two different primer sets, targeting the ...
Acanthamoeba mitochondrial 16S rDNA sequences: inferred
... with the large majority of AK cases. This genotype encompasses at least five species that have been differentiated largely on the basis of morphology. However, because it is generally agreed that morphology alone is unreliable for classification of this organism, there is a need for other markers th ...
... with the large majority of AK cases. This genotype encompasses at least five species that have been differentiated largely on the basis of morphology. However, because it is generally agreed that morphology alone is unreliable for classification of this organism, there is a need for other markers th ...
The Plant Cell
... MicroRNAs (miRNAs) are 21-nucleotide noncoding RNAs that have been identified in both animals and plants. Although in animals there is direct evidence implicating particular miRNAs in the control of developmental timing, to date it is not known whether plant miRNAs also play a role in regulating te ...
... MicroRNAs (miRNAs) are 21-nucleotide noncoding RNAs that have been identified in both animals and plants. Although in animals there is direct evidence implicating particular miRNAs in the control of developmental timing, to date it is not known whether plant miRNAs also play a role in regulating te ...
Document
... Four researchers from Lyon analyzed ALL published mRNA sequences of more than about 50 codons. All together they analyzed 90 sequences… ...
... Four researchers from Lyon analyzed ALL published mRNA sequences of more than about 50 codons. All together they analyzed 90 sequences… ...
The sequence of a gene encoding convicilin from pea
... legumin genes, but is not directly homologous with them. Comparison of this sequence with the 'inserted' sequence in soya-bean (Glycine max) conglycinin (a homologous vicilin-type protein) suggests that the two insertions were independent events. The 5' flanking sequence of the gene contains several ...
... legumin genes, but is not directly homologous with them. Comparison of this sequence with the 'inserted' sequence in soya-bean (Glycine max) conglycinin (a homologous vicilin-type protein) suggests that the two insertions were independent events. The 5' flanking sequence of the gene contains several ...
Evolution of Gene Order and Chromosome Number in
... within a pair of paralogous genes derived from genome duplication. They appear to be rare, because the chromosomes of the other species of Saccharomyces sensu stricto (S. paradoxus, S. bayanus, S. pastorianus) are generally collinear with those of S. cerevisiae. Ryu et al. (1996) mapped one legitima ...
... within a pair of paralogous genes derived from genome duplication. They appear to be rare, because the chromosomes of the other species of Saccharomyces sensu stricto (S. paradoxus, S. bayanus, S. pastorianus) are generally collinear with those of S. cerevisiae. Ryu et al. (1996) mapped one legitima ...
#2
... noncoding DNA sequences from human, chimpanzee, and baboon (Methods). Subsequently, human chromosomes were split into 1 Mb non-overlapping windows (referred to as loci). We retained 36 loci dispersed over 12 autosomal chromosomes, each of which contained sufficiently long triple alignments. The aver ...
... noncoding DNA sequences from human, chimpanzee, and baboon (Methods). Subsequently, human chromosomes were split into 1 Mb non-overlapping windows (referred to as loci). We retained 36 loci dispersed over 12 autosomal chromosomes, each of which contained sufficiently long triple alignments. The aver ...
American College of Medical Genetics and Genomics
... average proportion of the autosomal genome that is IBD in the offspring of related parents is given by the coefficient of inbreeding (F).4 For example, on average, 6.25% or 1/16th of the genome of offspring of first cousins (F = 1/16) is IBD. Although the coefficient of inbreeding provides a theoret ...
... average proportion of the autosomal genome that is IBD in the offspring of related parents is given by the coefficient of inbreeding (F).4 For example, on average, 6.25% or 1/16th of the genome of offspring of first cousins (F = 1/16) is IBD. Although the coefficient of inbreeding provides a theoret ...
current micro 40/5 - Bashan Foundation
... with cDNA derived from RT primer YAv3 as well as a combined amplificate for hoxY(part)/ORF3/hoxH(part) with cDNA derived from reverse primer HAv1 was demonstrated (Fig. 2). Thus, ORF8 and ORF3 are co-transcribed with the adjacent hox genes. In view of all these data, co-transcription of hoxFhoxUORF8 ...
... with cDNA derived from RT primer YAv3 as well as a combined amplificate for hoxY(part)/ORF3/hoxH(part) with cDNA derived from reverse primer HAv1 was demonstrated (Fig. 2). Thus, ORF8 and ORF3 are co-transcribed with the adjacent hox genes. In view of all these data, co-transcription of hoxFhoxUORF8 ...
Structure and functions of lampbrush chromosomes
... (Tsvetkov and Parfenov, 1994). This is particularly evident in hibernating amphibians. During the summer, when the animals are the most active, the transcriptional activity of LBCs is the highest, as well. In the autumn, LBCs’ activity abates. Nevertheless, this is not associated with morphological ...
... (Tsvetkov and Parfenov, 1994). This is particularly evident in hibernating amphibians. During the summer, when the animals are the most active, the transcriptional activity of LBCs is the highest, as well. In the autumn, LBCs’ activity abates. Nevertheless, this is not associated with morphological ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.