draft - University of Michigan
... Faster-X expression divergence To determine if these 4,851 genes showed greater expression divergence on the X chromosome than on autosomes, we used the nonparametric Spearman’s correlation coefficient (ρ) to compare levels of mRNA expression between X-linked and autosomal genes. Spearman’s ρ has pr ...
... Faster-X expression divergence To determine if these 4,851 genes showed greater expression divergence on the X chromosome than on autosomes, we used the nonparametric Spearman’s correlation coefficient (ρ) to compare levels of mRNA expression between X-linked and autosomal genes. Spearman’s ρ has pr ...
Long Noncoding RNA as a Regulator for Transcription
... activity in animal cells, and inhibition of their HAT activity (Fig. 2.4). Together with these data, it is suggested that expression of cyclin D1 gene could be repressed by pncRNAs through binding to TLS. This should be a mechanism like autorepression: a transcript from a gene represses its expressi ...
... activity in animal cells, and inhibition of their HAT activity (Fig. 2.4). Together with these data, it is suggested that expression of cyclin D1 gene could be repressed by pncRNAs through binding to TLS. This should be a mechanism like autorepression: a transcript from a gene represses its expressi ...
Introduction to RNA sequencing
... – Use aligner like BWA and a genome + junction database – Junction database needs to be tailored to read length • Or you can use a standard junction database for all read lengths and an aligner that allows substring alignments for the junctions only (e.g. ...
... – Use aligner like BWA and a genome + junction database – Junction database needs to be tailored to read length • Or you can use a standard junction database for all read lengths and an aligner that allows substring alignments for the junctions only (e.g. ...
Divergent Amphibian Species Nonclassical MHC Class I Lineages
... estimated to have roughly 20 XNC genes per haplotype by Southern blot analysis (12), in absence of genomic sequences their exact total number and the respective number of XNC genes within each subfamily is unknown. In contrast, the genome of the only diploid frog of the Xenopodinae subfamily, S. tro ...
... estimated to have roughly 20 XNC genes per haplotype by Southern blot analysis (12), in absence of genomic sequences their exact total number and the respective number of XNC genes within each subfamily is unknown. In contrast, the genome of the only diploid frog of the Xenopodinae subfamily, S. tro ...
Woods Hole – Zebrafish Genetics and Development Bioinformatics
... pattern consistent with the in situ data: expression in some tissues is absent, and many tissues express GFP where the gene is not normally expressed. How might you explain these results? You decide to make a new reporter line by BAC recombination: you will obtain a large (~200kb) chunk of genomic D ...
... pattern consistent with the in situ data: expression in some tissues is absent, and many tissues express GFP where the gene is not normally expressed. How might you explain these results? You decide to make a new reporter line by BAC recombination: you will obtain a large (~200kb) chunk of genomic D ...
An assessment of the risks associated with the
... bacterial resistance genes are with us. It would also be desirable to have selectable markers other than bacterial resistance genes with which to select desired transformed plant cells. Again, developments are in progress. In the meantime, it is worth emphasizing that a full inventory of the genetic ...
... bacterial resistance genes are with us. It would also be desirable to have selectable markers other than bacterial resistance genes with which to select desired transformed plant cells. Again, developments are in progress. In the meantime, it is worth emphasizing that a full inventory of the genetic ...
Molecular Testing and Clinical Diagnosis
... • Two common detection systems have been developed. – On glass slides, hybridization can be detected by fluorescence and spot color detection by a microarray scanner. – The silicone chip consists of electrodes, independently addressable via an electronic control system. Hybridization is detected by ...
... • Two common detection systems have been developed. – On glass slides, hybridization can be detected by fluorescence and spot color detection by a microarray scanner. – The silicone chip consists of electrodes, independently addressable via an electronic control system. Hybridization is detected by ...
Analysis of the histone H3 gene family in Arabidopsis and
... (Figure 3). Spatial expression of At1g19890 was analysed by in situ hybridisation to evaluate whether this gene encodes the male-gamete-specific histone H3 of Arabidopsis. In situ hybridisation could not detect transcripts of At1g19890 in uninucleate microspores or in early bicellular pollen, but cl ...
... (Figure 3). Spatial expression of At1g19890 was analysed by in situ hybridisation to evaluate whether this gene encodes the male-gamete-specific histone H3 of Arabidopsis. In situ hybridisation could not detect transcripts of At1g19890 in uninucleate microspores or in early bicellular pollen, but cl ...
Gene silencing: RNA makes RNA makes no
... the conventional RNA-dependent RNA polymerase motifs [3,6]. Perhaps these proteins represent a completely novel type of RNA-dependent RNA polymerase. Alternatively, it remains possible that the RNA-dependent RNA polymerase is a heteromeric complex in which the Qde-1 protein is a non-catalytic subuni ...
... the conventional RNA-dependent RNA polymerase motifs [3,6]. Perhaps these proteins represent a completely novel type of RNA-dependent RNA polymerase. Alternatively, it remains possible that the RNA-dependent RNA polymerase is a heteromeric complex in which the Qde-1 protein is a non-catalytic subuni ...
Widespread expression of the bovine Agouti gene results from at
... region II located between )52 and +417; and region III located between +1907 and +2605. Region I corresponding to the A promoter with at least 80% of identity suggests that this regulatory sequence is also functional in the two other species. The second region corresponds to the exon 1A and to the b ...
... region II located between )52 and +417; and region III located between +1907 and +2605. Region I corresponding to the A promoter with at least 80% of identity suggests that this regulatory sequence is also functional in the two other species. The second region corresponds to the exon 1A and to the b ...
Chapter 15 - HCC Learning Web
... Mendel’s dihybrid cross experiments produced offspring that had a combination of traits that did not match either parent in the P generation. If the P generation consists of a yellow-round seed parent (YYRR) crossed with a greenwrinkled seed parent (yyrr), all F1 plants have yellow-round seeds (Yy ...
... Mendel’s dihybrid cross experiments produced offspring that had a combination of traits that did not match either parent in the P generation. If the P generation consists of a yellow-round seed parent (YYRR) crossed with a greenwrinkled seed parent (yyrr), all F1 plants have yellow-round seeds (Yy ...
XistAR write up
... and sequencing RNA/DNA of male rats, since no inactivation is required in male rats, Tsix is only expressed from that mutant allele (Figure 1b). However, in the female versions of these rats, there appears to be an antisense transcript present, which can only be transcribed from the WT inactivated X ...
... and sequencing RNA/DNA of male rats, since no inactivation is required in male rats, Tsix is only expressed from that mutant allele (Figure 1b). However, in the female versions of these rats, there appears to be an antisense transcript present, which can only be transcribed from the WT inactivated X ...
Importance of integrons in the diffusion of resistance
... existence of genetic structures, called integrons, involved in the acquisition of resistance genes. These DNA elements have frequently been reported in multi-drug resistant strains isolated from animals and humans, and are located either on the bacterial chromosome or on broad-host-range plasmids. T ...
... existence of genetic structures, called integrons, involved in the acquisition of resistance genes. These DNA elements have frequently been reported in multi-drug resistant strains isolated from animals and humans, and are located either on the bacterial chromosome or on broad-host-range plasmids. T ...
Alnylam Licenses Intellectual Property from Cold Spring Harbor
... has built in the fundamental patents, technology, and know-how that underlie the discovery, development and commercialization of RNAi therapeutics Under the terms of the agreement, Alnylam receives a non-exclusive license from Cold Spring Harbor Laboratory to therapeutic uses of patent applications ...
... has built in the fundamental patents, technology, and know-how that underlie the discovery, development and commercialization of RNAi therapeutics Under the terms of the agreement, Alnylam receives a non-exclusive license from Cold Spring Harbor Laboratory to therapeutic uses of patent applications ...
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... PWS–AS region was accompanied by (or perhaps caused by) the fusion of SNRPN and UBE3A and the evolution of the ICR. Concurrently, SNURF and the snoRNAs were acquired, and MKRN3, MAGEL2 and NDN were retroposed into this domain. This occurred after the divergence of eutherians and marsupials 180 MYA, ...
... PWS–AS region was accompanied by (or perhaps caused by) the fusion of SNRPN and UBE3A and the evolution of the ICR. Concurrently, SNURF and the snoRNAs were acquired, and MKRN3, MAGEL2 and NDN were retroposed into this domain. This occurred after the divergence of eutherians and marsupials 180 MYA, ...
The home stretch, a first analysis of the nearly completed genome of
... contigs. The outcome of this assembly was that 52 whole genome contigs were assigned to CII. Subsequently, CII was reduced from 176 small contigs to being described by 10 large contigs. These were generated by the merging of the CII specific sequences and the 52 whole genome sequences. By default, t ...
... contigs. The outcome of this assembly was that 52 whole genome contigs were assigned to CII. Subsequently, CII was reduced from 176 small contigs to being described by 10 large contigs. These were generated by the merging of the CII specific sequences and the 52 whole genome sequences. By default, t ...
Low X/Y divergence in four pairs of papaya sex
... conservation among the aligned sequences of this X and Yh BAC pair, based on the density of crossing lines representing homologous sequences. The second inversion, which included the first inversion, involving the X BAC sequence from approximately 44 to 150 kb, and the corresponding Yh BAC sequence, ...
... conservation among the aligned sequences of this X and Yh BAC pair, based on the density of crossing lines representing homologous sequences. The second inversion, which included the first inversion, involving the X BAC sequence from approximately 44 to 150 kb, and the corresponding Yh BAC sequence, ...
DNA Chips: Genes to Disease
... Assessment Option: Use the Microarray Worksheet as a post-lab test to assess student understanding. *It is important that you facilitate a thorough discussion of microarray results with the students. Regarding the cancer cell example, often students have a naïve view that the only important genes wi ...
... Assessment Option: Use the Microarray Worksheet as a post-lab test to assess student understanding. *It is important that you facilitate a thorough discussion of microarray results with the students. Regarding the cancer cell example, often students have a naïve view that the only important genes wi ...
Genome position and gene amplification | SpringerLink
... and rare sites (RFS), which appear only in certain individuals. The sites are further distinguished by agents used to induce expression, which include aphidicolin, bromo-deoxyuridine (BrdU), 5-azacytidine and distamycin A. Folate stress caused by methotrexate exposure also induces a group of rare fr ...
... and rare sites (RFS), which appear only in certain individuals. The sites are further distinguished by agents used to induce expression, which include aphidicolin, bromo-deoxyuridine (BrdU), 5-azacytidine and distamycin A. Folate stress caused by methotrexate exposure also induces a group of rare fr ...
Cinteny is a flexible and efficient tool for analysis of synteny and
... between adjacent markers set to 1 Mb (denoted as DEFAGG). The minimum number of markers is set to two and paralogs contained within the largest synteny blocks are used, unless otherwise stated. All the graphical representations shown below are cross-linked, as well as linked to external resources, ...
... between adjacent markers set to 1 Mb (denoted as DEFAGG). The minimum number of markers is set to two and paralogs contained within the largest synteny blocks are used, unless otherwise stated. All the graphical representations shown below are cross-linked, as well as linked to external resources, ...
New techniques that could make germline genetic
... diabetes, heart disease and autism where a number of genes are involved, along with the environment. In the past a mouse with as few as three genes knocked out would have taken as many years to create; now it can be done in three weeks. CRISPR is also letting researchers get more out of other techno ...
... diabetes, heart disease and autism where a number of genes are involved, along with the environment. In the past a mouse with as few as three genes knocked out would have taken as many years to create; now it can be done in three weeks. CRISPR is also letting researchers get more out of other techno ...
The Euglena gracilis chloroplast rpoB gene
... We are interested in the relationship between chloroplast genes for RNA polymerase subunits and the known chloroplast polymerase activities. Antibodies against fusion proteins that contained fragments of the chloroplast genes rpoA from spinach, rpoB from tobacco, and rpoC2 from Euglena, were able to ...
... We are interested in the relationship between chloroplast genes for RNA polymerase subunits and the known chloroplast polymerase activities. Antibodies against fusion proteins that contained fragments of the chloroplast genes rpoA from spinach, rpoB from tobacco, and rpoC2 from Euglena, were able to ...
Small, Smaller, Smallest: The Origins and Evolution of Ancient Dual
... (BetaSymb ¼ Zinderia + Nasuia + Vidania), blue; symbiont loss, black dashed line; and symbiont replacement, green. Identified symbiont names are given at the tips. This schematic is not a full account of host relationships and symbiont associations, as many species and genera remain to be explored, ...
... (BetaSymb ¼ Zinderia + Nasuia + Vidania), blue; symbiont loss, black dashed line; and symbiont replacement, green. Identified symbiont names are given at the tips. This schematic is not a full account of host relationships and symbiont associations, as many species and genera remain to be explored, ...
The linear chromosome of the plant
... [6], while most other mycoplasmas have only one. Also, in contrast to most other mycoplasmas, phytoplasmas have resisted all attempts of cultivation in cell-free media, indicating that they have a different metabolism than other mycoplasmas and/or a greater reliance on their hosts. As a consequence, ...
... [6], while most other mycoplasmas have only one. Also, in contrast to most other mycoplasmas, phytoplasmas have resisted all attempts of cultivation in cell-free media, indicating that they have a different metabolism than other mycoplasmas and/or a greater reliance on their hosts. As a consequence, ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.