Journal of Molecular Microbiology and Biotechnology
... mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Duitman et al., 1999], suggesting that additional NRPS operons could be integrated in different ways in this area either as an insertion or as a substitution of existing NRPS ope ...
... mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Duitman et al., 1999], suggesting that additional NRPS operons could be integrated in different ways in this area either as an insertion or as a substitution of existing NRPS ope ...
Functional genomics of plant photosynthesis in
... proteins into multisubunit complexes, and indeed the Nac2 protein was shown to be part of a high molecular weight complex associated with non-polysomal RNA. RNA binding by Nac2 has not been shown, therefore Nac2 might bind RNA via an additional unidentified subunit. Candidates for this role include ...
... proteins into multisubunit complexes, and indeed the Nac2 protein was shown to be part of a high molecular weight complex associated with non-polysomal RNA. RNA binding by Nac2 has not been shown, therefore Nac2 might bind RNA via an additional unidentified subunit. Candidates for this role include ...
PDF
... and GH42 enzymes characterized in this study suggest that D. turgidum can utilize most plant-based polysaccharides except crystalline cellulose. The DNA polymerase I enzyme was also expressed and characterized. The pure enzyme showed improved amplification of long PCR targets compared to Taq polymer ...
... and GH42 enzymes characterized in this study suggest that D. turgidum can utilize most plant-based polysaccharides except crystalline cellulose. The DNA polymerase I enzyme was also expressed and characterized. The pure enzyme showed improved amplification of long PCR targets compared to Taq polymer ...
Manipulating the Plasmodium Genome
... transcribed by RNA polymerase II, consisting of a core promoter region controlled by upstream enhancer elements. However, they are functionally distinct from other eukaryotic promoters, as they do not function in mammalian COS cells and their sequences share no homology with any known transcription ...
... transcribed by RNA polymerase II, consisting of a core promoter region controlled by upstream enhancer elements. However, they are functionally distinct from other eukaryotic promoters, as they do not function in mammalian COS cells and their sequences share no homology with any known transcription ...
Molecular pathogenesis of feline leukemia virus
... Fig. 1. Concepts of retroviral insertional mutagenesis. The provirus contains long terminal repeats (LTRs) at both ends. LTRs can be subdivided into three regions: U3, R and U5. U3 contains the enhancer and promoter sequences that drive viral as well as cellular gene transcription. Retroviral gag, p ...
... Fig. 1. Concepts of retroviral insertional mutagenesis. The provirus contains long terminal repeats (LTRs) at both ends. LTRs can be subdivided into three regions: U3, R and U5. U3 contains the enhancer and promoter sequences that drive viral as well as cellular gene transcription. Retroviral gag, p ...
Evolutionary relationships and diversification of barhl genes within
... complexity to the developmental gene network that shapes organ formation. The genes of the barhl family encoding the homeobox transcription factors Barhl1 and Barhl2, have been shown to be expressed in more or less overlapping domains of the central nervous system and have partially redundant functi ...
... complexity to the developmental gene network that shapes organ formation. The genes of the barhl family encoding the homeobox transcription factors Barhl1 and Barhl2, have been shown to be expressed in more or less overlapping domains of the central nervous system and have partially redundant functi ...
SUPPLEMENTARY INFORMATION TABLE OF CONTENTS: 1
... translation frames and past stop codons. To tentatively reconstitute chimeric proteins from the analyzed DIRE, frame shifts were removed manually from the DNA sequences using the BER outputs to precisely determine the frame shift positions. This approach was used to generate a pseudogene for each DI ...
... translation frames and past stop codons. To tentatively reconstitute chimeric proteins from the analyzed DIRE, frame shifts were removed manually from the DNA sequences using the BER outputs to precisely determine the frame shift positions. This approach was used to generate a pseudogene for each DI ...
evolution and mechanism of translation in chloroplasts
... Chloroplasts are plant organelles that contain the entire machinery for the process of photosynthesis. In addition, chloroplasts possess their own genome, multiple copies of circular double-stranded DNA molecules, typically 150 kb in size, with over 100 different genes. According to the endosymbioti ...
... Chloroplasts are plant organelles that contain the entire machinery for the process of photosynthesis. In addition, chloroplasts possess their own genome, multiple copies of circular double-stranded DNA molecules, typically 150 kb in size, with over 100 different genes. According to the endosymbioti ...
Slides
... 10,000 base long RNA genome Converted to DNA and integrate into genome with help of RNA reverse transcriptase and integrase enzymes and long tandem repeats (LTRs) Transcribed into RNA and produce virus (example HIV) ...
... 10,000 base long RNA genome Converted to DNA and integrate into genome with help of RNA reverse transcriptase and integrase enzymes and long tandem repeats (LTRs) Transcribed into RNA and produce virus (example HIV) ...
Title: Evolution of dosage compensation in Anolis carolinensis, a
... the autosomes because of their unique inheritance patterns (Meisel and Connallon 2013). In species with XX/XY sex determination, females have two copies of the X chromosome, which means that, similar to the autosomes, deleterious recessive genes can be shielded from selection by dominant alleles in ...
... the autosomes because of their unique inheritance patterns (Meisel and Connallon 2013). In species with XX/XY sex determination, females have two copies of the X chromosome, which means that, similar to the autosomes, deleterious recessive genes can be shielded from selection by dominant alleles in ...
The Gene Gateway Workbook
... - Segments of DNA that encode proteins or other functional products are called genes. - Gene sequences are transcribed into messenger RNA intermediates (mRNA). - mRNA intermediates are translated into proteins that perform most life functions. ...
... - Segments of DNA that encode proteins or other functional products are called genes. - Gene sequences are transcribed into messenger RNA intermediates (mRNA). - mRNA intermediates are translated into proteins that perform most life functions. ...
early RNs, crossing over initiates, then synapsis begins Chiasmata
... Copyright ©2002 by the National Academy of Sciences ...
... Copyright ©2002 by the National Academy of Sciences ...
Annotation Instruction Sheet A. Information for Beginning Annotators
... the “Comparative Genomics” section. The “Conservation” and “Most Conserved” tracks rely on different programs that look for sequence conservation by comparing multiple species. These multi-species examinations can often be more sensitive that a typical BLAST search. Conservation of gene structure. T ...
... the “Comparative Genomics” section. The “Conservation” and “Most Conserved” tracks rely on different programs that look for sequence conservation by comparing multiple species. These multi-species examinations can often be more sensitive that a typical BLAST search. Conservation of gene structure. T ...
A pseudogene cluster in the leader region of the Euglena
... 16S rRNA leader region 1s a r e l i c of 16S-23S rRNA gene cluster having no functions for the genes. By what reasons the pseudogene cluster emerged? A large rRNA precursor containing both the 16S and 23S rRNA genes was observed in spinach (27,28) and Chlamidomonas (29) chloroplasts. A large rRNA of ...
... 16S rRNA leader region 1s a r e l i c of 16S-23S rRNA gene cluster having no functions for the genes. By what reasons the pseudogene cluster emerged? A large rRNA precursor containing both the 16S and 23S rRNA genes was observed in spinach (27,28) and Chlamidomonas (29) chloroplasts. A large rRNA of ...
E.coli
... Can we use this signal to deduce some more biological information ? We determined the most important metabolic networks in a (translationally biased) organism Can we determine genes belonging to minimal gene sets ? ...
... Can we use this signal to deduce some more biological information ? We determined the most important metabolic networks in a (translationally biased) organism Can we determine genes belonging to minimal gene sets ? ...
D. melanogaster - GEP Community Server
... source of evidence Resources that could be useful if the D. melanogaster evidence is ambiguous Whole genome alignments of 14 Drosophila species PhastCons and PhyloP conservation scores ...
... source of evidence Resources that could be useful if the D. melanogaster evidence is ambiguous Whole genome alignments of 14 Drosophila species PhastCons and PhyloP conservation scores ...
MGI-Guidelines for Nomenclature of Genes, Genetic Markers
... 2.4.4 Genes with Homologs in Other Species To aid interspecific comparison of genetic and other information, a gene that is identifiable as a homolog of an already named gene in another species can be named as "-like" "-homolog" or "related." (Note: this is not the same as "related sequence" which a ...
... 2.4.4 Genes with Homologs in Other Species To aid interspecific comparison of genetic and other information, a gene that is identifiable as a homolog of an already named gene in another species can be named as "-like" "-homolog" or "related." (Note: this is not the same as "related sequence" which a ...
RNA Polymerases
... regulation of their transcription. Some promoters such as the U6 small nuclear RNA (U6 snRNA ) and small RNA genes from the Epstein-Barr virus use only regulatory sequences upstream from their transcription start sites. The coding region of the U6 snRNA has a characteristic A box. However, this sequ ...
... regulation of their transcription. Some promoters such as the U6 small nuclear RNA (U6 snRNA ) and small RNA genes from the Epstein-Barr virus use only regulatory sequences upstream from their transcription start sites. The coding region of the U6 snRNA has a characteristic A box. However, this sequ ...
Genome duplications and accelerated evolution of
... the presence of six rather than the expected four Hox gene clusters. Subsequent extensive characterization of zebrafish Hox genes by Amores et al. (1998) showed that the zebrafish genome contains at least 48 Hox genes arranged in at least seven Hox clusters. Phylogenetic analysis and genetic mapping ...
... the presence of six rather than the expected four Hox gene clusters. Subsequent extensive characterization of zebrafish Hox genes by Amores et al. (1998) showed that the zebrafish genome contains at least 48 Hox genes arranged in at least seven Hox clusters. Phylogenetic analysis and genetic mapping ...
Stochastic Gene Expression:
... pattern of two alternative stable states of gene expression in different cells (Fig. 2). In both cases it is necessary to turn a randomly fluctuating level of P into a stable state of gene expression that is spatially either homogeneous or heterogeneous. In the case of color patterns, the level of P ...
... pattern of two alternative stable states of gene expression in different cells (Fig. 2). In both cases it is necessary to turn a randomly fluctuating level of P into a stable state of gene expression that is spatially either homogeneous or heterogeneous. In the case of color patterns, the level of P ...
Identification, cloning and sequence determination of genes specifying hexokinase A and B from yeast.
... of restriction sites had already been identified in these regions, they were used to obtain suitable DNA fragments from pBR328(EcoII) for dideoxy sequencing. These were cloned into M13mp7 or M13mp8 and provided the sequence information for the regions in Fig.3 shown above the HKB gene. As can be see ...
... of restriction sites had already been identified in these regions, they were used to obtain suitable DNA fragments from pBR328(EcoII) for dideoxy sequencing. These were cloned into M13mp7 or M13mp8 and provided the sequence information for the regions in Fig.3 shown above the HKB gene. As can be see ...
Efficient Ends-Out Gene Targeting In Drosophila
... Verification of crb::mEosFPKI and dArf6KO targeted alleles: To verify the crb::mEosFPKI allele, genomic DNA was extracted from homozygous mutant larvae or adult males for PCR verification. HJ58 and HJ59 flank the mEosFP insertion in crb::mEosFPKI. Wild type gDNA will yield a 185bp product, while gDN ...
... Verification of crb::mEosFPKI and dArf6KO targeted alleles: To verify the crb::mEosFPKI allele, genomic DNA was extracted from homozygous mutant larvae or adult males for PCR verification. HJ58 and HJ59 flank the mEosFP insertion in crb::mEosFPKI. Wild type gDNA will yield a 185bp product, while gDN ...
Ends-out, or replacement, gene targeting in Drosophila
... of the first exon and the intron and second exon) by PCR. This y fragment was cloned into the corresponding sites downstream of w⫹. The yellow rescue construct without FRTs. Two pairs of oligos, 5⬘AATTTAGGGATAACAGGGTAAT-3⬘, 5⬘-AATTATTACCCTGTTATCCCTA-3⬘, and 5⬘-TAGGGATAACAGGGTAATTGCA-3⬘, 5⬘-ATTACCCTG ...
... of the first exon and the intron and second exon) by PCR. This y fragment was cloned into the corresponding sites downstream of w⫹. The yellow rescue construct without FRTs. Two pairs of oligos, 5⬘AATTTAGGGATAACAGGGTAAT-3⬘, 5⬘-AATTATTACCCTGTTATCCCTA-3⬘, and 5⬘-TAGGGATAACAGGGTAATTGCA-3⬘, 5⬘-ATTACCCTG ...
introduction
... However, in other organisms, with the exception of certain green algae and leguminous plants, it is encoded by the mitochondrial genome (Gray, 1999; Palmer et al., 2000). The COXII protein of apicomplexan parasites contains two polypeptides which correspond to the amino terminal and the carboxyl ter ...
... However, in other organisms, with the exception of certain green algae and leguminous plants, it is encoded by the mitochondrial genome (Gray, 1999; Palmer et al., 2000). The COXII protein of apicomplexan parasites contains two polypeptides which correspond to the amino terminal and the carboxyl ter ...
The rapidly evolving field of plant centromeres
... CentO satellite (155 bp or 164 bp) interspersed with the Ty3/gypsy retroelement CRR (for centromere-specific retrotransposon of rice), as well as flanking regions that are rich in transposons, retroelements, and pseudogenes [7,8]. In maize, two sequenced centromere bacterial ...
... CentO satellite (155 bp or 164 bp) interspersed with the Ty3/gypsy retroelement CRR (for centromere-specific retrotransposon of rice), as well as flanking regions that are rich in transposons, retroelements, and pseudogenes [7,8]. In maize, two sequenced centromere bacterial ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.