Adaptive Gene Expression Divergence Inferred from Population
... allow us to polarize both expression and sequence evolution to particular lineages. Additionally, we used the sequence data to mask expression probes (which were developed using the D. melanogaster reference) with sequence mismatches in D. simulans and D. yakuba. This approach has the critical advan ...
... allow us to polarize both expression and sequence evolution to particular lineages. Additionally, we used the sequence data to mask expression probes (which were developed using the D. melanogaster reference) with sequence mismatches in D. simulans and D. yakuba. This approach has the critical advan ...
Intermediate Alleles - Huntington Society of Canada
... the disease but in which a case of HD has unexpectedly occurred. 1. No Family History: For decades, HD has been described as an inherited condition that is passed down in families from generation to generation. While this is most often the case, scientists have now determined that in some families, ...
... the disease but in which a case of HD has unexpectedly occurred. 1. No Family History: For decades, HD has been described as an inherited condition that is passed down in families from generation to generation. While this is most often the case, scientists have now determined that in some families, ...
Functional tests of enhancer conservation between
... Expression patterns of orthologous genes are often conserved, even between distantly related organisms, suggesting that once established, developmental programs can be stably maintained over long periods of evolutionary time. Because many orthologous transcription factors are also functionally conse ...
... Expression patterns of orthologous genes are often conserved, even between distantly related organisms, suggesting that once established, developmental programs can be stably maintained over long periods of evolutionary time. Because many orthologous transcription factors are also functionally conse ...
Exploitation of genes affecting meiotic non
... essential for SC assembly and normal synapsis (Caryl et al. 2000). In particular, ASY1 localisation suggests that it is required for SC morphogenesis rather than as a structural component of the SC itself (Armstrong et al. 2002). The DSY1 gene maps to chromosome 3, between genes GL-1 and HY-2, but i ...
... essential for SC assembly and normal synapsis (Caryl et al. 2000). In particular, ASY1 localisation suggests that it is required for SC morphogenesis rather than as a structural component of the SC itself (Armstrong et al. 2002). The DSY1 gene maps to chromosome 3, between genes GL-1 and HY-2, but i ...
High-Resolution Single-Copy Gene Fluorescence in Situ
... High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize ...
... High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize ...
Exercise 11 - Understanding the Output for a blastn Search
... Now that we have a better understanding of the organization of a BLAST report, we are ready to interpret the blastn results. From the graphical overview and the description list (Figures 3 and 4), we notice the top eight hits show much more significant alignments (with E-values of 0.0) compared to t ...
... Now that we have a better understanding of the organization of a BLAST report, we are ready to interpret the blastn results. From the graphical overview and the description list (Figures 3 and 4), we notice the top eight hits show much more significant alignments (with E-values of 0.0) compared to t ...
the art and design of genetic screens
... remains in widespread use. Indeed, the lacZ gene has now been incorporated into the genomes of all genetically tractable experimental organisms. Accordingly, we use the lac system as an example. Note, however, that many of the principles illustrated here can be adapted to other systems for which sim ...
... remains in widespread use. Indeed, the lacZ gene has now been incorporated into the genomes of all genetically tractable experimental organisms. Accordingly, we use the lac system as an example. Note, however, that many of the principles illustrated here can be adapted to other systems for which sim ...
Letter Gene Survival and Death on the Human Y
... FIG. 1. Pairwise synonymous substitution rate across chromosome X. The pairwise Xgene–Ygene and Xgene–Ypseudogene synonymous substitution rates (dS) are plotted across the entire X chromosome in 10 Mb sliding windows, sliding by 1 Mb. We observe a trend from smaller dS values to larger dS values as ...
... FIG. 1. Pairwise synonymous substitution rate across chromosome X. The pairwise Xgene–Ygene and Xgene–Ypseudogene synonymous substitution rates (dS) are plotted across the entire X chromosome in 10 Mb sliding windows, sliding by 1 Mb. We observe a trend from smaller dS values to larger dS values as ...
Review Article Viral Bacterial Artificial - diss.fu
... have become an important tool for herpesvirus research. In 1997, the first virus BAC system was developed by Messerle and colleagues for the murine cytomegalovirus (MCMV), one of the largest herpesviruses with a genome size of 230 kbp [14]. Shortly after the generation of the MCMV BAC, the technolog ...
... have become an important tool for herpesvirus research. In 1997, the first virus BAC system was developed by Messerle and colleagues for the murine cytomegalovirus (MCMV), one of the largest herpesviruses with a genome size of 230 kbp [14]. Shortly after the generation of the MCMV BAC, the technolog ...
Functional analysis of plastid DNA replication origins in tobacco by
... trnI intron, the second trnI exon, a spacer, the first trnA exon, and part of the trnA intron. If these extrachromosomal elements are replicated like bacterial plasmids, the sequence must contain an origin of replication. However, they could also be generated by excision from the plastid DNA by reco ...
... trnI intron, the second trnI exon, a spacer, the first trnA exon, and part of the trnA intron. If these extrachromosomal elements are replicated like bacterial plasmids, the sequence must contain an origin of replication. However, they could also be generated by excision from the plastid DNA by reco ...
RNA-guided gene drives can efficiently bias inheritance in wild yeast
... of the organism, but by increasing the odds that they themselves will be inherited. Because this inheritance advantage can counteract the fitness costs associated with the drive itself or with adjacent genes carried along with it, they are theoretically capable of 'driving' unrelated traits through ...
... of the organism, but by increasing the odds that they themselves will be inherited. Because this inheritance advantage can counteract the fitness costs associated with the drive itself or with adjacent genes carried along with it, they are theoretically capable of 'driving' unrelated traits through ...
Mapping of the Recessive White Locus and
... pmol of each primer, 0.2 mM each deoxynucleoside triphosphate (dNTP), 10 mM TrisⴢHCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, and 0.375 U of Taq polymerase. The PCR conditions were as follows: 94°C for 5 min followed by 35 cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 1 min, and a final extension at 72 ...
... pmol of each primer, 0.2 mM each deoxynucleoside triphosphate (dNTP), 10 mM TrisⴢHCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, and 0.375 U of Taq polymerase. The PCR conditions were as follows: 94°C for 5 min followed by 35 cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 1 min, and a final extension at 72 ...
Article The Pseudoautosomal Regions of the U/V Sex
... size (Spearman’s correlation test = 0.113, P = 0.598) whereas gene density and GC percentage increase with ...
... size (Spearman’s correlation test = 0.113, P = 0.598) whereas gene density and GC percentage increase with ...
Physical map of the aromatic amine and m-toluate
... present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in leng ...
... present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in leng ...
Barbara McClintock (1902-1992) - The Embryo Project Encyclopedia
... transposition were happening in the chromosomes. This breakage and transposition caused novel genetic combinations and thus unusual phenotypes in the offspring. McClintock first published her results on transposition in 1950 in an article titled, "The origin and behavior of mutable loci in maize," w ...
... transposition were happening in the chromosomes. This breakage and transposition caused novel genetic combinations and thus unusual phenotypes in the offspring. McClintock first published her results on transposition in 1950 in an article titled, "The origin and behavior of mutable loci in maize," w ...
CRISPR-Cas9 Mouse Toolbox
... List of plasmids described below: 1. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPAKrasG12D_HDRdonor-ITR (AAV-KPL) 2. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 3. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 4. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre- ...
... List of plasmids described below: 1. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPAKrasG12D_HDRdonor-ITR (AAV-KPL) 2. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 3. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 4. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre- ...
Accepted Version - CSIRO Research Publications Repository
... (Figure 1). However, in a self-pollinated ddm1 mutant, the BNS gene is methylated and stochastically silenced (Figure 1), indicating that bns is a loss of function epigenetic mutant. The BNS gene is flanked by a LINE (Long interspersed repeated element) sequence in a tail-to-tail orientation, and in ...
... (Figure 1). However, in a self-pollinated ddm1 mutant, the BNS gene is methylated and stochastically silenced (Figure 1), indicating that bns is a loss of function epigenetic mutant. The BNS gene is flanked by a LINE (Long interspersed repeated element) sequence in a tail-to-tail orientation, and in ...
Selection of Suitable Endogenous Reference Genes for Relative
... inserts. To accelerate the application of transformation in sugarcane, it is necessary and urgent to identify endogenous reference genes for transgenic sugarcane detection. Different molecular techniques such as Southern blotting [10], multiplex probe amplification and hybridization and microarray a ...
... inserts. To accelerate the application of transformation in sugarcane, it is necessary and urgent to identify endogenous reference genes for transgenic sugarcane detection. Different molecular techniques such as Southern blotting [10], multiplex probe amplification and hybridization and microarray a ...
Compressed q-gram Indexing for Highly Repetitive Biological
... sequences, which have many occurrences. In turn, this is particularly unfortunate because the search for short sequences is an extremely popular task in Computational Biology. For example, all the techniques that build on analyzing K-mers require that kind of search. Another example is the approxima ...
... sequences, which have many occurrences. In turn, this is particularly unfortunate because the search for short sequences is an extremely popular task in Computational Biology. For example, all the techniques that build on analyzing K-mers require that kind of search. Another example is the approxima ...
Bioinformatics Dr. Víctor Treviño Pabellón Tec
... the contribution of tht alignment to the msa. For example, if an extra copy of one of the sequences is added to the alignment project, then for sequence pairs that do not include that sequence will increase, indicating a lesser role because the contributions of that pair have been out-voted by the a ...
... the contribution of tht alignment to the msa. For example, if an extra copy of one of the sequences is added to the alignment project, then for sequence pairs that do not include that sequence will increase, indicating a lesser role because the contributions of that pair have been out-voted by the a ...
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.
... the plasmid has integrated. In particular, one must be aware of the possibility that integration has taken place at the chromosomal locus of the selectable marker, as it also provides homology for recombination. Indeed, there are situations where this event is desired, and can be accomplished by cle ...
... the plasmid has integrated. In particular, one must be aware of the possibility that integration has taken place at the chromosomal locus of the selectable marker, as it also provides homology for recombination. Indeed, there are situations where this event is desired, and can be accomplished by cle ...
Maternal and paternal genomes contribute equally to the
... form of imprinting in the Arabidopsis embryo. Genome-wide approaches similar to ours but looking much later after fertilization greatly expanded the list of genes with parent-of-origin-specific expression in the endosperm but did not identify such genes in embryos23,24. Thus, the imprinting-like phe ...
... form of imprinting in the Arabidopsis embryo. Genome-wide approaches similar to ours but looking much later after fertilization greatly expanded the list of genes with parent-of-origin-specific expression in the endosperm but did not identify such genes in embryos23,24. Thus, the imprinting-like phe ...
PDF
... [5], providing the opportunity to compare the mouse and human genomes. Comparing the human genome with the mouse genome can greatly help our understanding of both genomes. We used the BLASTN program [6] to compare the December 2001 golden path freeze of the human genome, which is also NCBI build 28, ...
... [5], providing the opportunity to compare the mouse and human genomes. Comparing the human genome with the mouse genome can greatly help our understanding of both genomes. We used the BLASTN program [6] to compare the December 2001 golden path freeze of the human genome, which is also NCBI build 28, ...
Comparative Genetics of Nucleotide Binding Site
... al. 1998). The substitution rates were corrected for multiple hits according to Dayhoff’s PAM matrix with the pairwise gap removal option active. RESULTS ...
... al. 1998). The substitution rates were corrected for multiple hits according to Dayhoff’s PAM matrix with the pairwise gap removal option active. RESULTS ...
network - bioinf leipzig
... The model based on experimental evidence in yeast organizes TFs in a stratified nature of three distinct layers: the top, core, and bottom layers. TFs within a layer are highly interconnected and share similar properties. TFs of the different layers regulate distinct sets of targets genes. The three ...
... The model based on experimental evidence in yeast organizes TFs in a stratified nature of three distinct layers: the top, core, and bottom layers. TFs within a layer are highly interconnected and share similar properties. TFs of the different layers regulate distinct sets of targets genes. The three ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.