TILLING. Traditional Mutagenesis Meets
... probability of being misled turns out to be vanishingly small. In other words, the two crosses that were carried out following mutagenesis unlink target and background mutations, so that it becomes highly unlikely for both a target and a background lesion to be homozygous in the same plants and only ...
... probability of being misled turns out to be vanishingly small. In other words, the two crosses that were carried out following mutagenesis unlink target and background mutations, so that it becomes highly unlikely for both a target and a background lesion to be homozygous in the same plants and only ...
Deciphering the developmental program in the ascidian
... Four mechanisms that can establish spatial patterns of gene expression Spatial patterns of gene expression might be established in a number of different ways during early development. It is possible to envisage that the expression patterns of some genes are determined via a two-step (rough and fine) ...
... Four mechanisms that can establish spatial patterns of gene expression Spatial patterns of gene expression might be established in a number of different ways during early development. It is possible to envisage that the expression patterns of some genes are determined via a two-step (rough and fine) ...
Efficiency of gene silencing in Arabidopsis
... by two independent events showing the strong reduction of function phenotype P2. First, we identified microarray elements showing differential expression (P < 0.05) in at least one of the experiments. Then, we identified elements that were down- or up-regulated in hIR and/or transitive events at an ...
... by two independent events showing the strong reduction of function phenotype P2. First, we identified microarray elements showing differential expression (P < 0.05) in at least one of the experiments. Then, we identified elements that were down- or up-regulated in hIR and/or transitive events at an ...
Opposing Activities of DRM and MES
... et al. 1991). Thus, the MES proteins cooperate to promote development of healthy germ cells by activating germline genes and repressing somatic genes. Another feature of gene regulation in C. elegans hermaphrodite germ cells is the significant dampening of transcription from the X chromosomes. Somati ...
... et al. 1991). Thus, the MES proteins cooperate to promote development of healthy germ cells by activating germline genes and repressing somatic genes. Another feature of gene regulation in C. elegans hermaphrodite germ cells is the significant dampening of transcription from the X chromosomes. Somati ...
Genetic recombination in plants
... hot spots and then determine whether these hot spots are genes. This process, however, is greatly complicated by the difficulty in distinguishing between genie and non-genie regions in complex genomes which are rich in retrotransposons and other repetitive sequences [26”,27]. The first step in testi ...
... hot spots and then determine whether these hot spots are genes. This process, however, is greatly complicated by the difficulty in distinguishing between genie and non-genie regions in complex genomes which are rich in retrotransposons and other repetitive sequences [26”,27]. The first step in testi ...
Molecular function - SGD-Wiki - Saccharomyces Genome Database
... How to stay current Basic org. (homepage, search, LSP) Tabs, access to detailed info (sequence, gene ontology, phenotype, interaction, expression and regulation) • Data analysis: GO tools, YeastMine basics and use-cases ...
... How to stay current Basic org. (homepage, search, LSP) Tabs, access to detailed info (sequence, gene ontology, phenotype, interaction, expression and regulation) • Data analysis: GO tools, YeastMine basics and use-cases ...
M2 RNA Pol Ⅰ genes
... They typically lie upstream of the gene and can have regulatory elements several kilobases away from the transcriptional start site. (Fig.11.19) ...
... They typically lie upstream of the gene and can have regulatory elements several kilobases away from the transcriptional start site. (Fig.11.19) ...
Author`s personal copy
... If a highly efficient method for mutant construction is developed, a genome-wide analysis of gene function is possible (Hammelmann and Soppa, 2008). It would not only accelerate the genome-wide identification of the genes responsible for PHA biosynthesis, but may also be used to genetically engineer ...
... If a highly efficient method for mutant construction is developed, a genome-wide analysis of gene function is possible (Hammelmann and Soppa, 2008). It would not only accelerate the genome-wide identification of the genes responsible for PHA biosynthesis, but may also be used to genetically engineer ...
Towards an accurate identification of mosaic genes and
... and bacteria (4). Two models of HGT have been considered in the literature (5). First, and the most popular of them, is the traditional model of complete HGT. It assumes that the transferred gene either supplants the orthologous gene of the recipient genome or, when the transferred gene is absent in ...
... and bacteria (4). Two models of HGT have been considered in the literature (5). First, and the most popular of them, is the traditional model of complete HGT. It assumes that the transferred gene either supplants the orthologous gene of the recipient genome or, when the transferred gene is absent in ...
localization of histone gene transcripts in newt lampbrush
... The recent availability of short DNA sequences cloned in plasmid or bacteriophage vectors has made possible the production, by nick-translation, of labelled probes of great sequence purity. Such probes, after denaturation, are ideally suited for hybridizing to RNA transcripts on lampbrush chromosome ...
... The recent availability of short DNA sequences cloned in plasmid or bacteriophage vectors has made possible the production, by nick-translation, of labelled probes of great sequence purity. Such probes, after denaturation, are ideally suited for hybridizing to RNA transcripts on lampbrush chromosome ...
Cloning and analysis of the mobile element gypsy from D. virilis
... The percent of the identity of the amino acid sequences of different genes cloned from D. melanogaster and D. virilis vary from 60% to more than 80% (16-23). Our finding of the active gypsy element in D. virilis with the degree of amino acid identity comparable to that of the 'usual' genes suggests ...
... The percent of the identity of the amino acid sequences of different genes cloned from D. melanogaster and D. virilis vary from 60% to more than 80% (16-23). Our finding of the active gypsy element in D. virilis with the degree of amino acid identity comparable to that of the 'usual' genes suggests ...
Daisy quorum drives for the genetic restoration of wild
... We reasoned that daisy drive could enable threshold-dependent elements to locally reach the threshold while releasing a tiny fraction of the organisms that would otherwise be required. Unfortunately, chromosomal translocations occur at low efficiency even when catalyzed by double-strand breaks17. Wh ...
... We reasoned that daisy drive could enable threshold-dependent elements to locally reach the threshold while releasing a tiny fraction of the organisms that would otherwise be required. Unfortunately, chromosomal translocations occur at low efficiency even when catalyzed by double-strand breaks17. Wh ...
unit-2 genetics of prokaryotes and eukaryotic
... also be produced in the laboratory by artificial methods. Based on the point in the life cycle involved, haploid production systems can be broadly classified in three main categories namely: i) parthenogenesis and apogamy, ii) chromosome elimination and iii) culture methods 1. Production of haploids ...
... also be produced in the laboratory by artificial methods. Based on the point in the life cycle involved, haploid production systems can be broadly classified in three main categories namely: i) parthenogenesis and apogamy, ii) chromosome elimination and iii) culture methods 1. Production of haploids ...
Fig. 1 - Repositorio Académico
... Dtg was controlled by Dpp and characterized a 524-bp enhancer that mediated expression in the dorsal midline, as well as, in the differentiated amnioserosa in transgenic reporter embryos. This enhancer contained a highly conserved region of 48-bp in which bioinformatic predictions and in vitro assay ...
... Dtg was controlled by Dpp and characterized a 524-bp enhancer that mediated expression in the dorsal midline, as well as, in the differentiated amnioserosa in transgenic reporter embryos. This enhancer contained a highly conserved region of 48-bp in which bioinformatic predictions and in vitro assay ...
$doc.title
... resistance genes that are effective against the leaf, stem and yellow rust pathogens, have been designated. The gene of importance for this study is leaf rust resistance gene Lr37, which maps to chromosome 2AS (Bariana & McIntosh, 1993). The gene is effective against leaf rust pathotypes UVPrt2 and ...
... resistance genes that are effective against the leaf, stem and yellow rust pathogens, have been designated. The gene of importance for this study is leaf rust resistance gene Lr37, which maps to chromosome 2AS (Bariana & McIntosh, 1993). The gene is effective against leaf rust pathotypes UVPrt2 and ...
New Construct Approaches for Efficient Gene Silencing in Plants
... Conventional gene silencing constructs have in some cases been used to simultaneously downregulate the expression of multiple genes (Halpin et al., 2001). The inverted repeats of such expression cassettes consisted of sense and antisense copies of two or more different gene fragments. Because transc ...
... Conventional gene silencing constructs have in some cases been used to simultaneously downregulate the expression of multiple genes (Halpin et al., 2001). The inverted repeats of such expression cassettes consisted of sense and antisense copies of two or more different gene fragments. Because transc ...
Ribosomal frameshifting in decoding antizyme mRNAs from yeast
... years. As pointed out previously we have been unable to amplify it from human genomic DNA (14). Based on these and other considerations, we now believe that this cDNA is a contaminant, most likely a mammalian antizyme 1 gene belonging to an unidentified rabbit or hare species. In our search for new a ...
... years. As pointed out previously we have been unable to amplify it from human genomic DNA (14). Based on these and other considerations, we now believe that this cDNA is a contaminant, most likely a mammalian antizyme 1 gene belonging to an unidentified rabbit or hare species. In our search for new a ...
In Silico method for identification of MHC class I
... This study is the first attempt to describe MHC class I like molecules of many mammals, including ones that have not previously been studied, in an in silico manner. The automated BLAST method we describe here consists of a blast search with the sequences of exon2 and exon3 which together form the ...
... This study is the first attempt to describe MHC class I like molecules of many mammals, including ones that have not previously been studied, in an in silico manner. The automated BLAST method we describe here consists of a blast search with the sequences of exon2 and exon3 which together form the ...
Discovery of Paralogous Nuclear Gene Sequences Coding for the
... taxa, and some synonymous changes may be informative at certain levels of the tree. Agosti, Jacobs, and DeSalle (1996) suggested that nucleic acid sequences and their translated amino acid codings could be combined to address this problem. We performed combined, as well as separate, amino acid and D ...
... taxa, and some synonymous changes may be informative at certain levels of the tree. Agosti, Jacobs, and DeSalle (1996) suggested that nucleic acid sequences and their translated amino acid codings could be combined to address this problem. We performed combined, as well as separate, amino acid and D ...
Reconstruction of a Functional Human Gene Network, with an
... contributing only weak effects to the disease. Pinpointing these disease genes within the myriad of susceptibility loci identified in linkage studies is difficult because these loci may contain hundreds of genes. However, in any disorder, most of the disease genes will be involved in only a few diff ...
... contributing only weak effects to the disease. Pinpointing these disease genes within the myriad of susceptibility loci identified in linkage studies is difficult because these loci may contain hundreds of genes. However, in any disorder, most of the disease genes will be involved in only a few diff ...
Transcription
... • RNA molecules are capable of both storing information and performing metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protein, just RNA performing ...
... • RNA molecules are capable of both storing information and performing metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protein, just RNA performing ...
Towards an accurate identification of mosaic genes and partial
... and bacteria (4). Two models of HGT have been considered in the literature (5). First, and the most popular of them, is the traditional model of complete HGT. It assumes that the transferred gene either supplants the orthologous gene of the recipient genome or, when the transferred gene is absent in ...
... and bacteria (4). Two models of HGT have been considered in the literature (5). First, and the most popular of them, is the traditional model of complete HGT. It assumes that the transferred gene either supplants the orthologous gene of the recipient genome or, when the transferred gene is absent in ...
Ancestry of neuronal monoamine transporters in the Metazoa
... structure and substrate kinetics may provide information on the origin and number of genes that encode SLC6 monoamine transporters in the Metazoa (Chen et al., 2004; Höglund et al., 2005). Do the genes encoding the transporter for each specific monoamine in the metazoan CNS derive from a common ance ...
... structure and substrate kinetics may provide information on the origin and number of genes that encode SLC6 monoamine transporters in the Metazoa (Chen et al., 2004; Höglund et al., 2005). Do the genes encoding the transporter for each specific monoamine in the metazoan CNS derive from a common ance ...
Lab #2
... • To test for under-enrichment of “black”, test for overenrichment of “red”. • Need to choose “background population” appropriately, e.g., if only portion of the total gene complement is queried (or available for annotation), only use that population as background. • To test for enrichment of more t ...
... • To test for under-enrichment of “black”, test for overenrichment of “red”. • Need to choose “background population” appropriately, e.g., if only portion of the total gene complement is queried (or available for annotation), only use that population as background. • To test for enrichment of more t ...
Identification and Analysis of Arabidopsis Expressed Sequence
... the JAW RNA is not known, it has been characterized as a ncRNA based on its expression in the presence of, and linkage to, an activation tag (Weigel et al., 2000). The other ncRNA candidates were implicated as ncRNAs from analyses of cDNAs corresponding to contiguous regions of Arabidopsis sequences ...
... the JAW RNA is not known, it has been characterized as a ncRNA based on its expression in the presence of, and linkage to, an activation tag (Weigel et al., 2000). The other ncRNA candidates were implicated as ncRNAs from analyses of cDNAs corresponding to contiguous regions of Arabidopsis sequences ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.