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AtREM1, a Member of a New Family of B3 Domain
AtREM1, a Member of a New Family of B3 Domain

... DNA sequences from the Arabidopsis Genome Initiative have revealed the existence of at least 23 sequences characterized by the presence of duplicated REM domains (Table I; Fig. 2). Many of them also have acidic domains and/or the heptad hydrophobic repeats at their C-terminal regions. Fourteen of th ...
The linear chromosome of the plant
The linear chromosome of the plant

... [6], while most other mycoplasmas have only one. Also, in contrast to most other mycoplasmas, phytoplasmas have resisted all attempts of cultivation in cell-free media, indicating that they have a different metabolism than other mycoplasmas and/or a greater reliance on their hosts. As a consequence, ...
miRNA pptx - NUS School of Computing
miRNA pptx - NUS School of Computing

... use the expression level of a known target genes for that specific intergenic miRNA If its target genes are consistently down-regulated, then we can assume that the expression level of the intergenic miRNA gene is up-regulated ...
Molecular Evolution in Nonrecombining Regions of the Drosophila
Molecular Evolution in Nonrecombining Regions of the Drosophila

... selection. However, in Drosophila melanogaster and its relatives, these studies have mostly focused on the small dot (fourth) chromosome, where recombination is minimal or completely absent (Haddrill et al. 2007; Arguello et al. 2010). This is because sequence data for most of the other nonrecombini ...
Chromosome-encoded gene cluster for the
Chromosome-encoded gene cluster for the

... a multi-component aniline dioxygenase and a LysR-type regulator, respectively, while the others (tadD1C1D2C2EFGIJKL) were expected to encode meta-cleavage pathway enzymes for catechol degradation. In addition, it was found that the gene cluster is surrounded by two IS1071 sequences, indicating that ...
Pseudogenes as regulators of biological function
Pseudogenes as regulators of biological function

... Regulation of mRNA stability The regulation of mRNA stability is one way in which gene expression can be controlled. The stability of an mRNA can be influenced by protein factors that bind at different locations in the RNA [38]. If a pseudogene has a high homology with the parent coding gene, includ ...
Connections between mRNA 3( end processing and transcription
Connections between mRNA 3( end processing and transcription

... the new 50 end generated by cleavage. As the polymerase continues transcribing, the excess transcript is degraded by Rat1, which eventually reaches the elongation complex and somehow induces termination. The homolog of yeast Rat1 is in higher eukaryotes is Xrn2. An RNAi knockdown of Xrn2 also leads ...
Gentile, Margaret: Computational Methods for the Design of PCR Primers for the Amplification of functional Markers from Environmental Samples
Gentile, Margaret: Computational Methods for the Design of PCR Primers for the Amplification of functional Markers from Environmental Samples

... and then design the primers within those regions. Previously, we discussed two attempts at designing primers in conserved regions (Braker et al, 1998 and Hallin and Lindgren, 1999). However, both of these attempts neglected to deal with the challenges which complicate the search for so called “conse ...
Phylogenetic relationship of phototrophic purple sulfur bacteria
Phylogenetic relationship of phototrophic purple sulfur bacteria

... green sulfur bacteria (GSB, i.e., Chlorobiaceae), and Heliobacteriaceae, as well as in some cyanobacteria under certain conditions. PSB represent a phylogenetic group that is clearly separated from all other anoxygenic photosynthetic bacteria, including the known gammaproteobacterial AAPB Congregiba ...
Lluís Millán Ariño GENOMIC DISTRIBUTION AND FUNCTIONAL SPECIFICITY OF
Lluís Millán Ariño GENOMIC DISTRIBUTION AND FUNCTIONAL SPECIFICITY OF

... Figure R.1. Expression of HA-tagged somatic histone H1 variants in breast cancer cells. (A) Proliferation assay to measure the effect of exogenous H1 variant expression. H1-HA expressing cell lines (GFP positive) were mixed 1:1 with parental T47D cells (GFP negative). Cells were split at the indicat ...
We need an optimality criterion to choose a best estimate (tree
We need an optimality criterion to choose a best estimate (tree

... organisms are so different? There are not likely to be many sequence homologies between bacteria, archaea, and eukaroytes. ...
Why Mitochondrial Genes are Most Often Found in Nuclei
Why Mitochondrial Genes are Most Often Found in Nuclei

... 1998). More to the point, an experimental system to study and quantify the transfer of sequences between mitochondrial and nuclear genomes in the yeast S. cerevisiae has been developed by Thorsness and Fox (1990, 1993). Thorsness and his collaborators have used this system to study a number of mutan ...
Finding Regulatory Motifs
Finding Regulatory Motifs

... Combinatorial Gene Regulation • A microarray experiment showed that when gene X is knocked out, 20 other genes are not expressed. • Motivating Question: How can one gene have such drastic effects? DNA Microarray ...
Saccharopolyspora erythraea that are involved
Saccharopolyspora erythraea that are involved

... protoplasts with the pWHM3 derivatives was then carried out as described by Weber & Losick (1988), except that R3M plates were used for growth on solid media. From several of the resulting thiostrepton-resistant transformants, the integrated plasmid was evicted by at least two rounds of nonselective ...
Transcription factories are nuclear subcompartments that remain in
Transcription factories are nuclear subcompartments that remain in

... because active genes migrate to shared subcompartments, then inhibition of transcription should result in the dissociation of distal genes. To test these possibilities we used the 3C assay to monitor associations between distal expressed genes Hbb-b1 and Eraf (25 Mb apart) as well as Eraf and Uros ( ...
Two enhancer regions in the mouse En-2 locus
Two enhancer regions in the mouse En-2 locus

... analyzed for lacZ activity as described below. The day on which a vaginal plug was observed was designated day 0.5 of gestation. In one of the transgenic lines, Tg4.35, analysis of DNA extracted from the founder revealed that the transgene had inserted at two different sites within the genome. These ...
Arabidopsis thaliana: A Model Plant for Genome Analysis
Arabidopsis thaliana: A Model Plant for Genome Analysis

... include simple procedures for chemical and insertional mutagenesis, efficient methods for performing crosses and introducing DNA through plant transformation, extensive collections of mutants with diverse phenotypes, and a variety of chromosome maps of mutant genes and molecular markers (8). The abs ...
Gene prediction and Genome Annotation
Gene prediction and Genome Annotation

... ProCoGen Dissemination Workshop, Riga, 5 nov 2013 “Conifer sequencing: basic concepts in conifer genomics” ...
ANSWER KEY
ANSWER KEY

... ___true____ d) Repressors are proteins that bind to DNA and turn off transcription of a gene or operon. ___false___ e) The term GENOTYPE describes the genetic constitution of an organism. ___true____ f) The site on the DNA to which RNA polymerase binds to start transcription is called the promoter. ...
Trans - Wiley
Trans - Wiley

... • Although many group II introns can selfsplice in vitro, many, if not all, require proteins in vivo to fold into the catalytically active structure. • Proteins required for splicing are either encoded by the introns themselves or by other genes of the host organism. ...
Nematode genome evolution
Nematode genome evolution

... an 11-gene region sequenced from P. pacificus chromosome III, 10/11 genes had orthologs on C. elegans chromosome III (Lee et al., 2003). This led Lee et al. to suggest that P. pacificus chromosome III and C. elegans chromosome III shared a common ancestor. If this is true, there must have been a lot ...
Overview of splicing relevant databases - Stamm
Overview of splicing relevant databases - Stamm

... every day and update of specialist databases such as alternative splicing databases is a crucial aspect. However, due to technical and time limitations, many databases are not regularly updated. The number of available information in alternative splicing databases depends on their update but also on ...
Commonly Used STR Markers
Commonly Used STR Markers

... – 2 to 6 bases repeated – Most commonly used for Forensics ...
Letter Neighboring Genes Show
Letter Neighboring Genes Show

... 2013). Genes showing nuclear colocalization tend to be regulated by similar transcriptional programs. Target genes of one TF tend to show nuclear proximity, and TFs with highly colocalized targets are expressed higher than those whose targets are not spatially clustered in yeast (Ben-Elazar et al. 2 ...
The Incompatible Desiderata of Gene Cluster Properties
The Incompatible Desiderata of Gene Cluster Properties

... than g. Note that the two definitions have switched roles: the local density is easily controlled by the parameter g for max-gap clusters but there is no way to constrain the local density of r-window clusters without also further constraining the maximum cluster length. This trade-off between glob ...
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Transposable element



A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.
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