Mutator Transposon in Maize and MULEs in the Plant Genome
... Mu insertions, especially germinally transmitted insertions [14]. Only two maize lines with active MuDR elements have been identified so far, but all maize lines carry MuDR elements derivatives, or homologous MuDR sequences (hMuDRs), whose coding sequences are 80%–99% identical to those of MuDR. Sur ...
... Mu insertions, especially germinally transmitted insertions [14]. Only two maize lines with active MuDR elements have been identified so far, but all maize lines carry MuDR elements derivatives, or homologous MuDR sequences (hMuDRs), whose coding sequences are 80%–99% identical to those of MuDR. Sur ...
chromosomes
... At the end of cell division, on the other hand, the fibers uncoil and extend as fine chromatin threads, which are not visible at light microscope ...
... At the end of cell division, on the other hand, the fibers uncoil and extend as fine chromatin threads, which are not visible at light microscope ...
Genes can be switched on and off by the protein CTCF
... or not. Dependent upon whether the DNA contained a binding site for CTCF so that CTCF could bind and function, or contained no such binding site, different signals were expected. However, I could not observe any signals. Therefor the set-up of this experiment has to be improved. I also wanted to fin ...
... or not. Dependent upon whether the DNA contained a binding site for CTCF so that CTCF could bind and function, or contained no such binding site, different signals were expected. However, I could not observe any signals. Therefor the set-up of this experiment has to be improved. I also wanted to fin ...
Ds - e-Acharya
... element, thus the P element can serve as a convenient marker. P elements do not utilize an RNA intermediate during transposition and can insert at many different positions in the Drosophila chromosome. The transposition of a P element is controlled by repressors encoded by the element. When P eleme ...
... element, thus the P element can serve as a convenient marker. P elements do not utilize an RNA intermediate during transposition and can insert at many different positions in the Drosophila chromosome. The transposition of a P element is controlled by repressors encoded by the element. When P eleme ...
Genetics Notes.notebook
... 2. What two molecules make the backbone of DNA? (these are the "handholds" of the ladder) ...
... 2. What two molecules make the backbone of DNA? (these are the "handholds" of the ladder) ...
MINI REVIEW The causes of Pseudomonas diversity
... and important source of innovation for bacterial evolution (Ochman et al., 2000). In Pseudomonas, population-genetic studies have shown that recombination occurs (Haubold & Rainey, 1996 ; Kiewitz & Tummler, 2000), but overall there is a pervasive clonal framework as evidenced by the repeated samplin ...
... and important source of innovation for bacterial evolution (Ochman et al., 2000). In Pseudomonas, population-genetic studies have shown that recombination occurs (Haubold & Rainey, 1996 ; Kiewitz & Tummler, 2000), but overall there is a pervasive clonal framework as evidenced by the repeated samplin ...
- ResearchOnline@JCU
... colon; one sibling has multiple serrated polyps and another a single large adenoma. Their mother developed pancreatic cancer and died before this study at age 50 years. On the paternal side, ...
... colon; one sibling has multiple serrated polyps and another a single large adenoma. Their mother developed pancreatic cancer and died before this study at age 50 years. On the paternal side, ...
Using bacterial biosensors to understand the genetic basis for
... when biosensors were exposed to PH ...
... when biosensors were exposed to PH ...
Concept of DNA and RNA
... the bacterial cell. In order to do all this, the virus must inject whatever is the viral genetic code into the host cell. Thus, people realized that the viral genetic code material had to be either its DNA or its protein capsid. Hershey and Chase sought an answer to the question, Is it the viral DNA ...
... the bacterial cell. In order to do all this, the virus must inject whatever is the viral genetic code into the host cell. Thus, people realized that the viral genetic code material had to be either its DNA or its protein capsid. Hershey and Chase sought an answer to the question, Is it the viral DNA ...
serious asthma should focus on the possible confounding role
... others acquire asthma later in life. Still other patients develop the disease in early childhood and suffer from asthma their entire life. In addition, ethnicity, sex and age affect asthma susceptibility and severity [8, 9]. How can we explain these different patterns? A challenging hypothesis may b ...
... others acquire asthma later in life. Still other patients develop the disease in early childhood and suffer from asthma their entire life. In addition, ethnicity, sex and age affect asthma susceptibility and severity [8, 9]. How can we explain these different patterns? A challenging hypothesis may b ...
The percentage of bacterial genes on leading versus
... (10). Furthermore, Rocha observed that the presence/absence of the RNA polymerase PolC in a genome is highly correlated with bacterial genomes having at least 70% their genes on the leading strand or not (11). Hu et al. proposed that replication-associated purine asymmetry may also contribute to the ...
... (10). Furthermore, Rocha observed that the presence/absence of the RNA polymerase PolC in a genome is highly correlated with bacterial genomes having at least 70% their genes on the leading strand or not (11). Hu et al. proposed that replication-associated purine asymmetry may also contribute to the ...
BioInformatics at FSU
... The Human Genome Project and numerous other genome projects have kept the data coming at alarming rates. As of April 2003, (50 years after the Watson-Crick double-helix!)16 Archaea, 128 Bacteria, and 10 Eukaryote complete, finished genomes; and 4 Vertebrate and 5 Plant essentially complete genome ma ...
... The Human Genome Project and numerous other genome projects have kept the data coming at alarming rates. As of April 2003, (50 years after the Watson-Crick double-helix!)16 Archaea, 128 Bacteria, and 10 Eukaryote complete, finished genomes; and 4 Vertebrate and 5 Plant essentially complete genome ma ...
Effects of Transition Mutations in the Regulatory
... is entirely contained within a 0-67kbp fragment bounded by EcoRI and BgZII sites (see Fig. 1). By cloning this fragment from plasmids to which the mutations had been transferred, sequencing from the EcoRI site, and comparing the results with the wild-type sequence, we were able to identify the mutat ...
... is entirely contained within a 0-67kbp fragment bounded by EcoRI and BgZII sites (see Fig. 1). By cloning this fragment from plasmids to which the mutations had been transferred, sequencing from the EcoRI site, and comparing the results with the wild-type sequence, we were able to identify the mutat ...
However, if
... ■ if the nucleus of only one parent was present, the larvae resembled that parent, but showed abnormalities. When an egg, whose nucleus had been removed, was fertilised with a sperm, the resulting sea urchin larvae showed characteristics similar to the male parent. However, they were smaller, had on ...
... ■ if the nucleus of only one parent was present, the larvae resembled that parent, but showed abnormalities. When an egg, whose nucleus had been removed, was fertilised with a sperm, the resulting sea urchin larvae showed characteristics similar to the male parent. However, they were smaller, had on ...
pdf
... Fig. 2. Evolution of the anticodon-binding pocket of PhProRS to charge different Af-tRNAPro anticodon variants. (A) Anticodon-binding site of ProRS in the crystal structure from T. thermophilus. The consensus sequence of the proline anticodon (GG), shown in magenta, interacts strongly with the amino ...
... Fig. 2. Evolution of the anticodon-binding pocket of PhProRS to charge different Af-tRNAPro anticodon variants. (A) Anticodon-binding site of ProRS in the crystal structure from T. thermophilus. The consensus sequence of the proline anticodon (GG), shown in magenta, interacts strongly with the amino ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... genomic DNA samples. We also prepared four oligonucleotide primers (5F, 5R, 3F, and 3R) for amplifying 5’-arm and 3‘-arm fragments respectively based on the HPO gene in pHPO-11172 from P. putida ATCC11172 as the main template, as shown in Table 1. In order to obtain the full open reading frame of ch ...
... genomic DNA samples. We also prepared four oligonucleotide primers (5F, 5R, 3F, and 3R) for amplifying 5’-arm and 3‘-arm fragments respectively based on the HPO gene in pHPO-11172 from P. putida ATCC11172 as the main template, as shown in Table 1. In order to obtain the full open reading frame of ch ...
Traversing the biological complexity in the hierarchy
... quantitatively varying intermediate traits. Each of these many intermediate traits is likely to be influenced by many gene products. Two sorts of studies to identify and characterize the genes that influence interindividual variation in these traits have been carried out (Sing et al. 1988). The top- ...
... quantitatively varying intermediate traits. Each of these many intermediate traits is likely to be influenced by many gene products. Two sorts of studies to identify and characterize the genes that influence interindividual variation in these traits have been carried out (Sing et al. 1988). The top- ...
Biotechnology
... a. For identification of a specific gene in a DNA extraction by hybridization to a DNA probe. b. For identification of a specific gene by hybridization to a DNA probe within live cells that have had their DNA denatured by heat. c. For identification of an mRNA within an RNA extraction by hybridizat ...
... a. For identification of a specific gene in a DNA extraction by hybridization to a DNA probe. b. For identification of a specific gene by hybridization to a DNA probe within live cells that have had their DNA denatured by heat. c. For identification of an mRNA within an RNA extraction by hybridizat ...
But what are genomic (additive) relationships?
... • Early use of markers used them to infer pedigrees or relationships • Gather markers, then reconstruct pedigrees, then construct A • In conservation genetics, molecular markers have often been used to estimate pedigree relationships • Either estimates of Axy , or estimates of « the most likely rel ...
... • Early use of markers used them to infer pedigrees or relationships • Gather markers, then reconstruct pedigrees, then construct A • In conservation genetics, molecular markers have often been used to estimate pedigree relationships • Either estimates of Axy , or estimates of « the most likely rel ...
sets of metaphors in multilevel cognitive models
... yazike y tekste", p.119-133); that just the metaphors play the key roles in various cognitive models (Lakoff, Johnson, 1980; MacCormac, 1985). Now, rapidly grows the interest to metaphors in the aspects of theories of cognition, logics, cognitive psychology, linguistics; last years, the research of ...
... yazike y tekste", p.119-133); that just the metaphors play the key roles in various cognitive models (Lakoff, Johnson, 1980; MacCormac, 1985). Now, rapidly grows the interest to metaphors in the aspects of theories of cognition, logics, cognitive psychology, linguistics; last years, the research of ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.