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... and chromosome breaks. So this experiment is performed on dying cells. Using the dnaBTS strain shows that the phenotypes being observed in rep strains are related to a general DNA replication problem, rather than due to some uncharacterized rep weirdness. There is more linear DNA in the absence of r ...
... and chromosome breaks. So this experiment is performed on dying cells. Using the dnaBTS strain shows that the phenotypes being observed in rep strains are related to a general DNA replication problem, rather than due to some uncharacterized rep weirdness. There is more linear DNA in the absence of r ...
Tracking the evolution of 3D gene organization demonstrates its
... {t} is the part of the read that was trimmed and {e} the acceptable error). We pooled the reads into their corresponding restriction fragments, and filtered reads that were either: more distant from the restriction site than the experiment’s molecule length; aligned to restriction fragments <100 bp ...
... {t} is the part of the read that was trimmed and {e} the acceptable error). We pooled the reads into their corresponding restriction fragments, and filtered reads that were either: more distant from the restriction site than the experiment’s molecule length; aligned to restriction fragments <100 bp ...
SAMPLE LITERATURE Please refer to included weblink for correct
... Many bacteria possess extra, non-essential genes on small circular pieces of double-stranded DNA in addition to their chromosomal DNA. These pieces of DNA, called plasmids, allow bacteria to exchange beneficial genes. For example, the gene that codes for ß-lactamase, an enzyme that provides antibioti ...
... Many bacteria possess extra, non-essential genes on small circular pieces of double-stranded DNA in addition to their chromosomal DNA. These pieces of DNA, called plasmids, allow bacteria to exchange beneficial genes. For example, the gene that codes for ß-lactamase, an enzyme that provides antibioti ...
From Genetics to DNA
... distinction made above between sense and antisense strands by having overlapping genes. In these cases, some DNA sequences do double duty, encoding one protein when read 5′ to 3′ along one strand, and a second protein when read in the opposite direction (still 5′ to 3′) along the other strand. In ba ...
... distinction made above between sense and antisense strands by having overlapping genes. In these cases, some DNA sequences do double duty, encoding one protein when read 5′ to 3′ along one strand, and a second protein when read in the opposite direction (still 5′ to 3′) along the other strand. In ba ...
Envelope gene sequences encoding variable regions 3 and 4 are
... envelope glycoprotein determinants involved in macrophage tropism, chimeric infectious molecular clones were constructed containing envelope gene sequences from isolates that had been propagated in peripheral blood mononuclear cells (PBMC). The progeny virus was examined for growth in PBMC and bone ...
... envelope glycoprotein determinants involved in macrophage tropism, chimeric infectious molecular clones were constructed containing envelope gene sequences from isolates that had been propagated in peripheral blood mononuclear cells (PBMC). The progeny virus was examined for growth in PBMC and bone ...
in vitro
... Some of the drawbacks of these methods are: •The inserted DNA randomly integrates into the genome •The eggs must be harvested & fertilized in vitro •More than one copy of the gene may get into the genome ...
... Some of the drawbacks of these methods are: •The inserted DNA randomly integrates into the genome •The eggs must be harvested & fertilized in vitro •More than one copy of the gene may get into the genome ...
in vitro
... Some of the drawbacks of these methods are: •The inserted DNA randomly integrates into the genome •The eggs must be harvested & fertilized in vitro •More than one copy of the gene may get into the genome ...
... Some of the drawbacks of these methods are: •The inserted DNA randomly integrates into the genome •The eggs must be harvested & fertilized in vitro •More than one copy of the gene may get into the genome ...
Leukaemia Section dic(9;20)(p11 13;q11) -
... sites, but does effect the expression of certain downstream PAX5 target genes. Furthermore, transfected vectors encoding the PAX5-C20ORF112 are able to suppress the transcriptional activity of wildtype PAX5, leading to the inhibition of B-cell development. Sequence analysis and genomic quantitative ...
... sites, but does effect the expression of certain downstream PAX5 target genes. Furthermore, transfected vectors encoding the PAX5-C20ORF112 are able to suppress the transcriptional activity of wildtype PAX5, leading to the inhibition of B-cell development. Sequence analysis and genomic quantitative ...
Deoxyribonucleic Acid Base Composition of Acetic
... aceti (paradoxus)was grown on 50 % (v/v) red wine, 0-5yo Yeastex, 1 % DL-lactate Ca salt, 2 yoCaCO,, 2.5 yoagar. Sometimes other media were used, e.g. with sorbitol instead of glucose. No differences in DNA base composition were then observed. Pseudomonasfluorescens was incubated for one day a t 30” ...
... aceti (paradoxus)was grown on 50 % (v/v) red wine, 0-5yo Yeastex, 1 % DL-lactate Ca salt, 2 yoCaCO,, 2.5 yoagar. Sometimes other media were used, e.g. with sorbitol instead of glucose. No differences in DNA base composition were then observed. Pseudomonasfluorescens was incubated for one day a t 30” ...
CURRICULUM VITAE - Oxford University Statistics
... relative, the chimpanzee. Our group, and others, have been instrumental in demonstrating that recombination occurs very unevenly across the genome, that most recombination occurs in narrow hotspots, and that recombination rates vary rapidly over evolutionary time. I have developed methods to detect ...
... relative, the chimpanzee. Our group, and others, have been instrumental in demonstrating that recombination occurs very unevenly across the genome, that most recombination occurs in narrow hotspots, and that recombination rates vary rapidly over evolutionary time. I have developed methods to detect ...
The methylcitric acid pathway in Ralstonia eutropha
... growth on propionic acid and levulinic acid. From the molecular, physiological and enzymic analysis of these mutants it was concluded that in this bacterium propionic acid is metabolized via the methylcitric acid pathway. The genes encoding enzymes of this pathway are organized in a cluster in the o ...
... growth on propionic acid and levulinic acid. From the molecular, physiological and enzymic analysis of these mutants it was concluded that in this bacterium propionic acid is metabolized via the methylcitric acid pathway. The genes encoding enzymes of this pathway are organized in a cluster in the o ...
Slide 1
... The standard vertebrate order is shared by 398 species (including humans). There are many other species with unique gene orders. Some species conserve gene order over 100s of millions of years. Others get scrambled in a few million. Still to do (new project) : - estimate relative rates of different ...
... The standard vertebrate order is shared by 398 species (including humans). There are many other species with unique gene orders. Some species conserve gene order over 100s of millions of years. Others get scrambled in a few million. Still to do (new project) : - estimate relative rates of different ...
Engineering of steroid biotransformation in rhodococcus van
... chosen in a comparative study of several Rhodococcus strains as a suitable (i.e. genetically accessible and sterol degrading) parent strain for this study (Chapter 2). An electrotransformation protocol allowed transformation efficiencies of up to 1·106 transformants per µg of plasmid DNA (pMVS301). ...
... chosen in a comparative study of several Rhodococcus strains as a suitable (i.e. genetically accessible and sterol degrading) parent strain for this study (Chapter 2). An electrotransformation protocol allowed transformation efficiencies of up to 1·106 transformants per µg of plasmid DNA (pMVS301). ...
DNA
... o Streptococcus pneumonia bacteria was working to find cure for pneumonia o harmless live bacteria (“rough”) mixed with heat-killed pathogenic bacteria (“smooth”) causes fatal disease in ...
... o Streptococcus pneumonia bacteria was working to find cure for pneumonia o harmless live bacteria (“rough”) mixed with heat-killed pathogenic bacteria (“smooth”) causes fatal disease in ...
it is not in our genes
... molecular genetic research: genome-wide association . . . In just a year’s time, GWA studies have come to dominate the gene-hunting literature” (Plomin & Davis, 2009). There are some three billion base pairs* on the double helix of the DNA of each individual, of which 99% are the same from person to ...
... molecular genetic research: genome-wide association . . . In just a year’s time, GWA studies have come to dominate the gene-hunting literature” (Plomin & Davis, 2009). There are some three billion base pairs* on the double helix of the DNA of each individual, of which 99% are the same from person to ...
Full Paper - Biotechniques.org
... gene has been determined in several organisms including humans and mice, but has yet to be sequenced in rats. The reported coding region size of the mouse PEX5 gene is approximately 1.9kb. The sequence of a 1.2kb portion of the PEX5 homologue in rat was determined by using RT-PCR on mRNA extracted f ...
... gene has been determined in several organisms including humans and mice, but has yet to be sequenced in rats. The reported coding region size of the mouse PEX5 gene is approximately 1.9kb. The sequence of a 1.2kb portion of the PEX5 homologue in rat was determined by using RT-PCR on mRNA extracted f ...
A natural chimeric yeast containing genetic material from three species
... addition to four arbitrary bases, thus allowing restriction digestion. Genomic DNA was prepared from liquid yeast cultures (Hoffman & Winston, 1987). For each DNA preparation, 10 µl of a 100-fold dilution was used as PCR template. The PCR was performed on a Stratagene Robocycler 40 for 25 cycles of ...
... addition to four arbitrary bases, thus allowing restriction digestion. Genomic DNA was prepared from liquid yeast cultures (Hoffman & Winston, 1987). For each DNA preparation, 10 µl of a 100-fold dilution was used as PCR template. The PCR was performed on a Stratagene Robocycler 40 for 25 cycles of ...
Characterization of Complementary DNA Encoding the Precursor for
... associated peptide, cGAP, shows little if any relation to mGAP; five residues may be appropriately aligned, with no more than two contiguously. However, a single base insertion within cGAP generates a potential peptide sequence with a significant block of homology to a conserved region of the mammal ...
... associated peptide, cGAP, shows little if any relation to mGAP; five residues may be appropriately aligned, with no more than two contiguously. However, a single base insertion within cGAP generates a potential peptide sequence with a significant block of homology to a conserved region of the mammal ...
Table S10. List of the Databases and Software used in the H
... http://crick.genes.nig.ac.jp/homology/clustalw.shtml ...
... http://crick.genes.nig.ac.jp/homology/clustalw.shtml ...
LIMITED DNA SYNTHESIS IN THE ABSENCE OF PROTEIN
... that there are between three and five rounds of replication during the S period of Physarum. These rounds are probably not clearly delineated by discontinuous periods of protein synthesis, but they probably arise from the average replication of a large number of individual units which vary in the du ...
... that there are between three and five rounds of replication during the S period of Physarum. These rounds are probably not clearly delineated by discontinuous periods of protein synthesis, but they probably arise from the average replication of a large number of individual units which vary in the du ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.