11357_2014_9648_MOESM1_ESM
... System (Qiagen, Venlo, The Netherlands) and CpG methylation was analysed with the provided software. Primer sequence used are listed in supplemental table 5. ...
... System (Qiagen, Venlo, The Netherlands) and CpG methylation was analysed with the provided software. Primer sequence used are listed in supplemental table 5. ...
Effective transfer of chromosomes carrying leaf rust resistance
... with resistance to leaf rust. The aim of this study was: (1) to characterize the chromosome composition of the hybrids (BC2F2 to BC2F5) of Ae. tauschii × triticale hybrids; (2) to identify the D-genome DNA markers which are linked with leaf rust resistance in the hybrids; and (3) to evaluate the sta ...
... with resistance to leaf rust. The aim of this study was: (1) to characterize the chromosome composition of the hybrids (BC2F2 to BC2F5) of Ae. tauschii × triticale hybrids; (2) to identify the D-genome DNA markers which are linked with leaf rust resistance in the hybrids; and (3) to evaluate the sta ...
Back-translation for discovering distant protein homologies
... plain BLAST [10, 11] alignment approach: BLASTN on DNA and mRNA, or BLASTX on mRNA and proteins, applicable only when the DNA sequences are sufficiently similar. BLASTX programs, although capable of insightful results thanks to the six frame translations, have the limitation of not being able to tra ...
... plain BLAST [10, 11] alignment approach: BLASTN on DNA and mRNA, or BLASTX on mRNA and proteins, applicable only when the DNA sequences are sufficiently similar. BLASTX programs, although capable of insightful results thanks to the six frame translations, have the limitation of not being able to tra ...
Human Apolipoprotein A-l-C-lll Gene Complex is Located on
... that they bind and transport lipid in the blood stream. Structurally the apolipoproteins that have been sequenced have all possessed amhipathic alpha-helical regions capable of interacting with aqueous and nonpolar environments.1 In 1977 Barker and Dayhoff30, using amino-acid sequence data available ...
... that they bind and transport lipid in the blood stream. Structurally the apolipoproteins that have been sequenced have all possessed amhipathic alpha-helical regions capable of interacting with aqueous and nonpolar environments.1 In 1977 Barker and Dayhoff30, using amino-acid sequence data available ...
Package `LDheatmap`
... alleles at each SNP same as dbSNPalleles chromosome (chr7 for all) physical position strand (all "+") ...
... alleles at each SNP same as dbSNPalleles chromosome (chr7 for all) physical position strand (all "+") ...
Biosafety Protocol Registration Form
... Yes No 2. Experiment extends the host range. Yes No D-4: Experiments involving whole animals in which the animal’s genome has been altered by stable introduction of recombinant DNA, or DNA derived therefrom, into the germ-line (transgenic animals), and experiments involving viable recombinant DNA-mo ...
... Yes No 2. Experiment extends the host range. Yes No D-4: Experiments involving whole animals in which the animal’s genome has been altered by stable introduction of recombinant DNA, or DNA derived therefrom, into the germ-line (transgenic animals), and experiments involving viable recombinant DNA-mo ...
The complete nucleotide sequence of the RNA coding for the
... The processing of picornaviral proteins is a complex process and appears highly variable between different members of the family. However, the general genomic organisation and the primary cleavage products derived from the polyprotein are similar, (1, 19) with one important exception. The region to ...
... The processing of picornaviral proteins is a complex process and appears highly variable between different members of the family. However, the general genomic organisation and the primary cleavage products derived from the polyprotein are similar, (1, 19) with one important exception. The region to ...
An Introduction to Genetic Analysis Chapter21 Extranuclear Genes
... cells can obtain ATP by fermentation, which does not rely on the mitochondrial oxidative phosphorylation system, so yeast with these drastically deleted genotypes can survive, albeit at a reduced activity level. Petites in which part of the mtDNA has been deleted regenerate full-sized mtDNA molecule ...
... cells can obtain ATP by fermentation, which does not rely on the mitochondrial oxidative phosphorylation system, so yeast with these drastically deleted genotypes can survive, albeit at a reduced activity level. Petites in which part of the mtDNA has been deleted regenerate full-sized mtDNA molecule ...
Structure of B-DNA with Cations Tethered in the Major Groove†
... DDD4+ with native DDD structures. Here, we compare DDD4+ with a series of DDD structures determined from crystals obtained in the presence of various cations, in our laboratory. The members of this ensemble were determined independently and give an indication of the minimum accuracy of the helical p ...
... DDD4+ with native DDD structures. Here, we compare DDD4+ with a series of DDD structures determined from crystals obtained in the presence of various cations, in our laboratory. The members of this ensemble were determined independently and give an indication of the minimum accuracy of the helical p ...
1 Introduction
... proliferative state of the cell cycle (Woessner et al, 1991). In quiescent and differentiated cell populations the isoform IIα is not found expressed in an amount detectable by immunoblotting or immunohistochemical studies (Woessner et al, 1991; Boege et al, 1995). However, after a release of cells ...
... proliferative state of the cell cycle (Woessner et al, 1991). In quiescent and differentiated cell populations the isoform IIα is not found expressed in an amount detectable by immunoblotting or immunohistochemical studies (Woessner et al, 1991; Boege et al, 1995). However, after a release of cells ...
How to determine whether a strain will undergo senescence. Background
... Standard wild type laboratory Neurospora strains and most strains obtained from nature are able to grow indefinitely. Vegetatively propogated cultures are virtually immortal, with a potential for unlimited growth. Deleterious mutant genes and gene combinations are expected eventually to accumulate a ...
... Standard wild type laboratory Neurospora strains and most strains obtained from nature are able to grow indefinitely. Vegetatively propogated cultures are virtually immortal, with a potential for unlimited growth. Deleterious mutant genes and gene combinations are expected eventually to accumulate a ...
Part III: Laboratory – Electrophoresis
... Harvest plant tissue for PCR as soon as the difference in phenotype between the plants becomes obvious. This should be about 1-3 weeks after planting and will depend upon the light and temperature conditions. As described in the introduction, the plants that are homozygous for the bz-2 mutation have ...
... Harvest plant tissue for PCR as soon as the difference in phenotype between the plants becomes obvious. This should be about 1-3 weeks after planting and will depend upon the light and temperature conditions. As described in the introduction, the plants that are homozygous for the bz-2 mutation have ...
- Department of Molecular Biology and Genetics, Faculty
... Publication of genome sequences permit the design of ‘short’ DNA oligonucleotides (25 70 nts) that are sequencfe specific for particular genes. These single stranded oligos can then by spotted onto the microarray slide and hybridised in ‘ two colour competitive hybridisations’ Oligo probes ...
... Publication of genome sequences permit the design of ‘short’ DNA oligonucleotides (25 70 nts) that are sequencfe specific for particular genes. These single stranded oligos can then by spotted onto the microarray slide and hybridised in ‘ two colour competitive hybridisations’ Oligo probes ...
Report The Derived FOXP2 Variant of Modern Humans Was Shared
... from Neandertals. For this scenario to be likely, the relevant haplotype would have needed to be at a considerable frequency in the ancestral population in order to obtain the relatively high frequencies in Neandertals. However, the higher the frequency of a variant is before it becomes positively s ...
... from Neandertals. For this scenario to be likely, the relevant haplotype would have needed to be at a considerable frequency in the ancestral population in order to obtain the relatively high frequencies in Neandertals. However, the higher the frequency of a variant is before it becomes positively s ...
See a Sample
... Early comparative genomics Comparative genomics prior to obtaining full genome sequence: Genome size Compared DNA content among species ...
... Early comparative genomics Comparative genomics prior to obtaining full genome sequence: Genome size Compared DNA content among species ...
benfey_ch10
... Early comparative genomics Comparative genomics prior to obtaining full genome sequence: Genome size Compared DNA content among species ...
... Early comparative genomics Comparative genomics prior to obtaining full genome sequence: Genome size Compared DNA content among species ...
supplementary methods
... BamHI-MluNI fragment from BGT145 that contains the 5' A-globin intron 2 with the Oct-1 site. Third, this new BGT147 hybrid intron 2 was released by digestion with BamHI and EcoRI and cloned between the BamHI and EcoRI sites of BGT64. BGT147 encodes fully wild-type Aglobin. BGT156 was created by li ...
... BamHI-MluNI fragment from BGT145 that contains the 5' A-globin intron 2 with the Oct-1 site. Third, this new BGT147 hybrid intron 2 was released by digestion with BamHI and EcoRI and cloned between the BamHI and EcoRI sites of BGT64. BGT147 encodes fully wild-type Aglobin. BGT156 was created by li ...
Deletion of a Disease Resistance Nucleotide-Binding
... If these genes were to assort independently, ⵑ8 susceptible plants (rps4rps6) should have been identified. These results suggest that two genes are linked with a 2 probability of 0.0046. To distinguish the race specificity of Rps4 and Rps6, we infected HARO4272 (Rps4) and L89-1581 (Rps6) with five ...
... If these genes were to assort independently, ⵑ8 susceptible plants (rps4rps6) should have been identified. These results suggest that two genes are linked with a 2 probability of 0.0046. To distinguish the race specificity of Rps4 and Rps6, we infected HARO4272 (Rps4) and L89-1581 (Rps6) with five ...
DNA methylation profile in human CD4+ T cells identifies
... chromatin accessibility and regulating gene expression. This epigenetic mechanism plays an important role in T cell function. We used genome-wide methylation profiling to characterize the DNA methylome in primary human CD4+ T cells. We found that only 5% of CpG islands are methylated in CD4+ T cells ...
... chromatin accessibility and regulating gene expression. This epigenetic mechanism plays an important role in T cell function. We used genome-wide methylation profiling to characterize the DNA methylome in primary human CD4+ T cells. We found that only 5% of CpG islands are methylated in CD4+ T cells ...
Limitations of Pseudogenes in Identifying Gene Losses
... 86 not overlapping a D. melanogaster coding sequence. These two sets are further explored in the next two sections. Query genes that do not overlap a D. melanogaster coding sequence The set of 86 non-melanogaster query genes that hit part of the D. melanogaster genome but do not overlap with any D. ...
... 86 not overlapping a D. melanogaster coding sequence. These two sets are further explored in the next two sections. Query genes that do not overlap a D. melanogaster coding sequence The set of 86 non-melanogaster query genes that hit part of the D. melanogaster genome but do not overlap with any D. ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.