Sequencing for all. - Thermo Fisher Scientific
... making it simple and affordable. It’s about openly sharing your methods and data so people can not only evaluate the technology, but build on it. Ion Torrent has opened its protocols, datasets, and source code to the world to enable the community to drive application development and help make sequen ...
... making it simple and affordable. It’s about openly sharing your methods and data so people can not only evaluate the technology, but build on it. Ion Torrent has opened its protocols, datasets, and source code to the world to enable the community to drive application development and help make sequen ...
Global Properties of the Metabolic Map of
... The EcoCyc database characterizes the known network of Escherichia coli small-molecule metabolism. Here we present a computational analysis of the global properties of that network, which consists of 744 reactions that are catalyzed by 607 enzymes. The reactions are organized into 131 pathways. Of t ...
... The EcoCyc database characterizes the known network of Escherichia coli small-molecule metabolism. Here we present a computational analysis of the global properties of that network, which consists of 744 reactions that are catalyzed by 607 enzymes. The reactions are organized into 131 pathways. Of t ...
Chpt7_RepairDNA.doc
... MUTATION AND REPAIR OF DNA Most biological molecules have a limited lifetime. Many proteins, lipids and RNAs are degraded when they are no longer needed or damaged, and smaller molecules such as sugars are metabolized to compounds to make or store energy. In contrast, DNA is the most stable biologic ...
... MUTATION AND REPAIR OF DNA Most biological molecules have a limited lifetime. Many proteins, lipids and RNAs are degraded when they are no longer needed or damaged, and smaller molecules such as sugars are metabolized to compounds to make or store energy. In contrast, DNA is the most stable biologic ...
Printable version - Chromosome 18 Registry and Research Society
... each chromosome has a waistband constriction somewhere along its length. This is called a centromere and it makes the chromosomes look as if they have a long and a short arm. The convention is that the chromosome is always oriented with the short arm on top. You will also see that this particular ka ...
... each chromosome has a waistband constriction somewhere along its length. This is called a centromere and it makes the chromosomes look as if they have a long and a short arm. The convention is that the chromosome is always oriented with the short arm on top. You will also see that this particular ka ...
Gene Section XPC (xeroderma pigmentosum, complementation group C) Atlas of Genetics and Cytogenetics
... Repair (NER) repair capacity, but the residual repair has been shown to occur specifically in transcribed genes. It is very likely that the XPC-HR23B complex is the principal damage recognition complex i.e. essential for the recognition of DNA lesions in the genome. Binding of XPC-HR23B to a DNA les ...
... Repair (NER) repair capacity, but the residual repair has been shown to occur specifically in transcribed genes. It is very likely that the XPC-HR23B complex is the principal damage recognition complex i.e. essential for the recognition of DNA lesions in the genome. Binding of XPC-HR23B to a DNA les ...
DNA Hybridization: A Decade of Molecular Discourse in Hominoid
... hybridization. Not only are these points essential for an lillderstanding of any discussion on the contribution ofDNA hybridization to hominoid phylogeny, but also an awareness of the above two points enables one to appreciate the controversy that plagues this area of research. As mentioned before, ...
... hybridization. Not only are these points essential for an lillderstanding of any discussion on the contribution ofDNA hybridization to hominoid phylogeny, but also an awareness of the above two points enables one to appreciate the controversy that plagues this area of research. As mentioned before, ...
Document
... 7.1 Chromosomes and Phenotype • Female mammals have an XX genotype. – Expression of sex-linked genes is similar to autosomal genes in females. – X chromosome inactivation randomly “turns off” one X chromosome – ensures that females, like males, have one functional copy of the X chromosome in each b ...
... 7.1 Chromosomes and Phenotype • Female mammals have an XX genotype. – Expression of sex-linked genes is similar to autosomal genes in females. – X chromosome inactivation randomly “turns off” one X chromosome – ensures that females, like males, have one functional copy of the X chromosome in each b ...
Some psychrophiles Abstract
... psychrophilic microorganisms, which encode multiple copies of tRNA species for biosynthesis of all amino acids, as well as an increased variety and number of chaperones [18]. For example, four copies of the chaperone DnaJ are encoded on the genome of Psychromonas ingrahamii, and the psychrophilic Al ...
... psychrophilic microorganisms, which encode multiple copies of tRNA species for biosynthesis of all amino acids, as well as an increased variety and number of chaperones [18]. For example, four copies of the chaperone DnaJ are encoded on the genome of Psychromonas ingrahamii, and the psychrophilic Al ...
View PDF - SciTechnol
... Foster City, CA, USA). Sequences were compared with the wild type sequence of the FANCI gene (AC_000178.1) using DNAMAN software. ...
... Foster City, CA, USA). Sequences were compared with the wild type sequence of the FANCI gene (AC_000178.1) using DNAMAN software. ...
Question paper - Unit F215/01 - Control, genomes and
... Fig. 3.2 (on the next page) shows the end parts of the sequences of seven of these different length fragments, labelled 1 to 7. The end parts of the sequences for fragments 1 to 4 are complete but those for fragments 5 to 7 are not. These seven fragments correspond to the last seven peaks on the rig ...
... Fig. 3.2 (on the next page) shows the end parts of the sequences of seven of these different length fragments, labelled 1 to 7. The end parts of the sequences for fragments 1 to 4 are complete but those for fragments 5 to 7 are not. These seven fragments correspond to the last seven peaks on the rig ...
Applied and Environmental Microbiology
... Archaeoglobus fulgidus (16). Form IV is designated a RubisCOlike protein, as its sequence is most closely related to RubisCO but it is not involved in the Calvin cycle. The large subunit of form I RubisCO is encoded by the cbbL gene (18). The cbbL gene is 1,400 bp long and thus is large enough for u ...
... Archaeoglobus fulgidus (16). Form IV is designated a RubisCOlike protein, as its sequence is most closely related to RubisCO but it is not involved in the Calvin cycle. The large subunit of form I RubisCO is encoded by the cbbL gene (18). The cbbL gene is 1,400 bp long and thus is large enough for u ...
The DNA sequence and biology of human chromosome 19
... duplications as detected by the whole-genome shotgun sequence detection method. The underlying pairwise alignments of segmental duplications (>90% >1kb) are depicted as a function of % identity below the horizontal line. Different colors correspond to the location of the pairwise alignment on differ ...
... duplications as detected by the whole-genome shotgun sequence detection method. The underlying pairwise alignments of segmental duplications (>90% >1kb) are depicted as a function of % identity below the horizontal line. Different colors correspond to the location of the pairwise alignment on differ ...
Mechanical separation of the complementary strands of DNA
... Going back from D to A (not shown), the two single strands reannealed, and a new measurement cycle could be engaged. The force signal acquired during this return phase may have differed from the signal obtained during the opening, with instabilities and partial nonreproducibility. However, upon open ...
... Going back from D to A (not shown), the two single strands reannealed, and a new measurement cycle could be engaged. The force signal acquired during this return phase may have differed from the signal obtained during the opening, with instabilities and partial nonreproducibility. However, upon open ...
KOD -Plus- Mutagenesis Kit
... Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable i ...
... Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable i ...
Recombinant DNA technology and molecular cloning
... recombinant DNA technology. One of the first recombinant DNA molecules to be engineered was a hybrid of phage λ and the SV40 mammalian DNA virus genome. In 1974 the first eukaryotic gene was cloned. Amplified ribosomal RNA (rRNA) genes or “ribosomal DNA” (rDNA) from the South African clawed frog Xen ...
... recombinant DNA technology. One of the first recombinant DNA molecules to be engineered was a hybrid of phage λ and the SV40 mammalian DNA virus genome. In 1974 the first eukaryotic gene was cloned. Amplified ribosomal RNA (rRNA) genes or “ribosomal DNA” (rDNA) from the South African clawed frog Xen ...
UNIFR Rusconi 2002
... Basic understanding of 'genes and genomes': what is a gene, how many genes, molecular biology dogma genetic diseases, environmental factors, Essential concepts on 'molecular medicine' applications and problems, ageing as the major disease Genomics and other 'omics' essential technologies Application ...
... Basic understanding of 'genes and genomes': what is a gene, how many genes, molecular biology dogma genetic diseases, environmental factors, Essential concepts on 'molecular medicine' applications and problems, ageing as the major disease Genomics and other 'omics' essential technologies Application ...
CHAPTER 13 DNA manipulation
... regularly interspaced short palindromic repeats. These are short repeated segments of DNA, with each repeated segment being separated by a length of spacer DNA. ...
... regularly interspaced short palindromic repeats. These are short repeated segments of DNA, with each repeated segment being separated by a length of spacer DNA. ...
A Drosophila Third Chromosome Minute Locus Encodes
... M ( 3 ) 9 5 A ( P l a c 9 2 ) r y / T M b R , T h stock and that the element has inserted in region 94F/95A ofthe third chromosome (Figure 1A). A 5.6kb RamHI fragment containing the gene disrupted by the P element insertion (see below) was also used for i n s i t u hybridization to salivary gland po ...
... M ( 3 ) 9 5 A ( P l a c 9 2 ) r y / T M b R , T h stock and that the element has inserted in region 94F/95A ofthe third chromosome (Figure 1A). A 5.6kb RamHI fragment containing the gene disrupted by the P element insertion (see below) was also used for i n s i t u hybridization to salivary gland po ...
Thermostable glycerol kinase from a
... studied with respect to its functions and structures. This enzyme acts in the rate-limiting step in the glycerol utilization pathway and its catalytic activity is closely regulated by two allosteric inhibitors, IIIglc [EIIAGlc according to a new nomenclature proposed by Saier and Reizer (1992)] of t ...
... studied with respect to its functions and structures. This enzyme acts in the rate-limiting step in the glycerol utilization pathway and its catalytic activity is closely regulated by two allosteric inhibitors, IIIglc [EIIAGlc according to a new nomenclature proposed by Saier and Reizer (1992)] of t ...
Multifractal characterisation of length sequences of coding and
... in the complete genome. This integer sequence is named coding length sequence. (ii) Order all lengths of noncoding segments according to the order of noncoding segments in the complete genome. This integer sequence is named noncoding length sequence. Yu and Anh [20] proposed a time series model for ...
... in the complete genome. This integer sequence is named coding length sequence. (ii) Order all lengths of noncoding segments according to the order of noncoding segments in the complete genome. This integer sequence is named noncoding length sequence. Yu and Anh [20] proposed a time series model for ...
Bacterial genospecies that are not ecologically
... [4– 6]. Alternatively, these clusters might reflect the underlying ecological niches provided by the environment, and this idea has been developed into the ecotype model, in which genotypic clusters map onto ecological niches and periodic selective sweeps purge genetic variation within each niche se ...
... [4– 6]. Alternatively, these clusters might reflect the underlying ecological niches provided by the environment, and this idea has been developed into the ecotype model, in which genotypic clusters map onto ecological niches and periodic selective sweeps purge genetic variation within each niche se ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.