Study of the arginine repressor in different organisms
... ArgRTnp was crystallized in the absence and in the presence of 10 mM L-arginine. A data set to a resolution of 2.1 Å was collected from a crystal of the aporepressor grown from 0.8 M K, Natartrate, 0.1 M Na-HEPES pH 7.5 (Crystal Screen, Hampton Research). Glycerol was used as a cryoprotectant. The c ...
... ArgRTnp was crystallized in the absence and in the presence of 10 mM L-arginine. A data set to a resolution of 2.1 Å was collected from a crystal of the aporepressor grown from 0.8 M K, Natartrate, 0.1 M Na-HEPES pH 7.5 (Crystal Screen, Hampton Research). Glycerol was used as a cryoprotectant. The c ...
The Incompatible Desiderata of Gene Cluster Properties
... constrain the local density of r-window clusters without also further constraining the maximum cluster length. This trade-off between global and local density gives a simple illustration of how it can be difficult to design a cluster definition that satisfies our basic intuitions about cluster prope ...
... constrain the local density of r-window clusters without also further constraining the maximum cluster length. This trade-off between global and local density gives a simple illustration of how it can be difficult to design a cluster definition that satisfies our basic intuitions about cluster prope ...
S4 File
... rs3769818, two A/G transitions in CASP8 intron 9 with equal minor allele frequency (MAF) for A=0.308/671, which were present in blood samples from 11 and 79 patients, respectively, but not in matched tumors; AMPL4050069285 identified rs80075693, again a C/T transition in TAOK1 intron 19 (MAF unknown ...
... rs3769818, two A/G transitions in CASP8 intron 9 with equal minor allele frequency (MAF) for A=0.308/671, which were present in blood samples from 11 and 79 patients, respectively, but not in matched tumors; AMPL4050069285 identified rs80075693, again a C/T transition in TAOK1 intron 19 (MAF unknown ...
Yeast genome evolution-the origin of the species
... coding for an enzyme (dihydro-orotate dehydrogenase) in the uracil biosynthesis pathway, which was gained in the Saccharomycotina lineage by horizontal transfer from a bacterium resembling Lactococcus lactis [22,23]. URA1 initially coexisted with the distantly related native gene URA9, and eventuall ...
... coding for an enzyme (dihydro-orotate dehydrogenase) in the uracil biosynthesis pathway, which was gained in the Saccharomycotina lineage by horizontal transfer from a bacterium resembling Lactococcus lactis [22,23]. URA1 initially coexisted with the distantly related native gene URA9, and eventuall ...
RunzheimerSpr13
... Dan Herman in the UWEC Biology Department, we are investigating the role of Mbp1 in this morphological conversion. To further our understanding of Mbp1 in C. albicans, we expressed C. albicans MBP1in S. cerevisiae, and our results indicate it suppresses a synthetically lethal mbp1⁻/ swi4⁻ phenotype. ...
... Dan Herman in the UWEC Biology Department, we are investigating the role of Mbp1 in this morphological conversion. To further our understanding of Mbp1 in C. albicans, we expressed C. albicans MBP1in S. cerevisiae, and our results indicate it suppresses a synthetically lethal mbp1⁻/ swi4⁻ phenotype. ...
Altering substrate specificity of catechol 2,3
... EcoRI and HindIII endonuclease sites of pUC19 with T4 DNA ligase (Fermentas). The ligation mixture was then transformed into competent E. coli DH5α and plated on LB medium supplemented with ampicillin (100 µg ml–1), IPTG (79.2 µg ml–1) and X-gal (32 µg ml–1). After transformation ampicillin-resistan ...
... EcoRI and HindIII endonuclease sites of pUC19 with T4 DNA ligase (Fermentas). The ligation mixture was then transformed into competent E. coli DH5α and plated on LB medium supplemented with ampicillin (100 µg ml–1), IPTG (79.2 µg ml–1) and X-gal (32 µg ml–1). After transformation ampicillin-resistan ...
Molecular Evidence for Vector Implication of Onchocerca lupi in Los
... Onchocerca is a genus of filarial parasites with worldwide distribution and is typically associated with ungulates, including horses and cattle. The causative agent of human “river blindness” also resides within the same genus (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). Historically, canids wer ...
... Onchocerca is a genus of filarial parasites with worldwide distribution and is typically associated with ungulates, including horses and cattle. The causative agent of human “river blindness” also resides within the same genus (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). Historically, canids wer ...
Chapter 1 A Perspective on Human Genetics
... Shotgun Cloning (used by private project) • Genomic library prepared • No genetic or physical maps are created • Restriction enzymes are used to cut DNA, and overlapping fragments are created • Clones selected at random from each library and sequenced • Assembler software programs organize informati ...
... Shotgun Cloning (used by private project) • Genomic library prepared • No genetic or physical maps are created • Restriction enzymes are used to cut DNA, and overlapping fragments are created • Clones selected at random from each library and sequenced • Assembler software programs organize informati ...
Unit 3
... “brother.” Fraternal twins are like any brothers and sisters, except that they are born on the same day. They each have some the same genes as their parents. But they do not share an identical genetic makeup. Fraternal twins may resemble each other, but they usually will not be mistaken for each oth ...
... “brother.” Fraternal twins are like any brothers and sisters, except that they are born on the same day. They each have some the same genes as their parents. But they do not share an identical genetic makeup. Fraternal twins may resemble each other, but they usually will not be mistaken for each oth ...
Molecular Analysis of the Coprinus cinereus Mating Type A Factor
... has been described previously (PUKKILA and CASSELTON 1991). Partial MboI fragments of genomic DNA isolated from strain Ok7 (A43,B43) weresize fractionated and fragments 38-50 kbp were ligated intothe polylinker BamHI site. After ligation, cosmid clones were packaged withGigapack I1 Gold (Stratagene) ...
... has been described previously (PUKKILA and CASSELTON 1991). Partial MboI fragments of genomic DNA isolated from strain Ok7 (A43,B43) weresize fractionated and fragments 38-50 kbp were ligated intothe polylinker BamHI site. After ligation, cosmid clones were packaged withGigapack I1 Gold (Stratagene) ...
Full Paper - Biotechniques.org
... have been used to barcode algae, including 23S plastid rRNA (Sherwood and Presting 2007), 18S rRNA (Zimmerman et al. 2011), and rbcL (Paul et al. 2000). The rbcL gene is of particular interest because it codes for the large subunit of the protein ribulose-1,5-bisphosphate carboxylase oxygenase (RuB ...
... have been used to barcode algae, including 23S plastid rRNA (Sherwood and Presting 2007), 18S rRNA (Zimmerman et al. 2011), and rbcL (Paul et al. 2000). The rbcL gene is of particular interest because it codes for the large subunit of the protein ribulose-1,5-bisphosphate carboxylase oxygenase (RuB ...
BIOLOGY (THEORY) – 2008
... In the technique of organ transplant lateral corneal transplantation has been successfully done. But organ like kidney heart & lever it is difficult, it is because different blood supply and immune system of the reciepient and donor. But now by the process of Cryogeneses (preserving organs under fre ...
... In the technique of organ transplant lateral corneal transplantation has been successfully done. But organ like kidney heart & lever it is difficult, it is because different blood supply and immune system of the reciepient and donor. But now by the process of Cryogeneses (preserving organs under fre ...
Efficient Screening of CRISPR/Cas9
... improve the screening process in Drosophila to help identify broods that were more likely to contain CRISPR-induced events. Several studies have shown that multiple CRISPR events can occur in a single cell (Cong et al. 2013; Wang et al. 2013), enabling the development of a co-CRISPR or coconversion ...
... improve the screening process in Drosophila to help identify broods that were more likely to contain CRISPR-induced events. Several studies have shown that multiple CRISPR events can occur in a single cell (Cong et al. 2013; Wang et al. 2013), enabling the development of a co-CRISPR or coconversion ...
Supporting Information Legends Supporting Figure 1. Amino acid
... (A) Schematic diagrams of the AGO2 and the mutated AGO2 genes. The first half of the AGO2 genes is indicated. The black horizontal lines above or below the AGO2 diagrams correspond to the regions amplified by genomic PCR. The location of the AGO2 5th intron probe is indicated with double lines. PstI ...
... (A) Schematic diagrams of the AGO2 and the mutated AGO2 genes. The first half of the AGO2 genes is indicated. The black horizontal lines above or below the AGO2 diagrams correspond to the regions amplified by genomic PCR. The location of the AGO2 5th intron probe is indicated with double lines. PstI ...
Identification of disease genes by whole genome
... to this chromosomal region (2). Although de novo translocations have been most widely used for the mapping and identification of disease genes, small deletions have been instrumental for cloning the genes for familial adenomatous polyposis (3), retinoblastoma (4), WAGR syndrome (5) and a number of o ...
... to this chromosomal region (2). Although de novo translocations have been most widely used for the mapping and identification of disease genes, small deletions have been instrumental for cloning the genes for familial adenomatous polyposis (3), retinoblastoma (4), WAGR syndrome (5) and a number of o ...
Barley Cbf3 Gene Identification, Expression Pattern, and Map Location
... 8.7-kb region on chromosome 4 (Gilmour et al., 1998; Liu et al., 1998), and lesser studied genes on chromosome 5 (CBF4/DREBID; Nakamura et al., 1998; Thomashow et al., 2001) and chromosome 1 (DREB1E and DREB1F; Sakuma et al., 2002). The expression patterns of these genes have notable differences. Fo ...
... 8.7-kb region on chromosome 4 (Gilmour et al., 1998; Liu et al., 1998), and lesser studied genes on chromosome 5 (CBF4/DREBID; Nakamura et al., 1998; Thomashow et al., 2001) and chromosome 1 (DREB1E and DREB1F; Sakuma et al., 2002). The expression patterns of these genes have notable differences. Fo ...
Substitution Rates in a New Silene latifolia Sex
... and c2B12–2, designed using the sequence of the c2B12 cDNA clone, amplified an approximately 1-kb region from all male and female S. latifolia and S. dioica individuals. In addition, male S. latifolia individuals had a second PCR product, which was slightly longer (fig. 1). Malespecificity of the lo ...
... and c2B12–2, designed using the sequence of the c2B12 cDNA clone, amplified an approximately 1-kb region from all male and female S. latifolia and S. dioica individuals. In addition, male S. latifolia individuals had a second PCR product, which was slightly longer (fig. 1). Malespecificity of the lo ...
Catabolic Plasmids - UQ eSpace
... based primarily on plasmids derived from clinical isolates it does not reflect plasmids from a wide range of environmental isolates. Indeed incompatibility groupings have been developed for Pseudomonas (IncP1, P2...P14) and other organisms whose plasmids do not fit into this incompatibility grouping. ...
... based primarily on plasmids derived from clinical isolates it does not reflect plasmids from a wide range of environmental isolates. Indeed incompatibility groupings have been developed for Pseudomonas (IncP1, P2...P14) and other organisms whose plasmids do not fit into this incompatibility grouping. ...
—1— User Guide © Copyright 2009 Robert C. Edgar, all rights
... (inter) once for the entire genome, then the intra-chromosome simulator (intra) once for each chromosome. This process is called a cycle. The output from one cycle can be used as input to another cycle. It is generally better to run many short cycles rather than one or a few long cycles as longer cy ...
... (inter) once for the entire genome, then the intra-chromosome simulator (intra) once for each chromosome. This process is called a cycle. The output from one cycle can be used as input to another cycle. It is generally better to run many short cycles rather than one or a few long cycles as longer cy ...
Structure and expression of the PHO80 gene of Saccharomyces
... In yeast, the repression of acid phosphatase under high phosphate growth conditions requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis ...
... In yeast, the repression of acid phosphatase under high phosphate growth conditions requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.