Molecular cloning and functional characterisation of a glucose
... C. albicans [19], containing chromosome I fragments, was screened using the 1-kb EcoRI-HindIII subfragment of pAV1 (pAV4, Fig. 1A) as a probe. Southern analysis gave two positive bands of 4.2 kb and 2 kb from fosmids 11D6 and 18B4. The 4.2-kb HincII fragment was further subcloned into pUC18 at the S ...
... C. albicans [19], containing chromosome I fragments, was screened using the 1-kb EcoRI-HindIII subfragment of pAV1 (pAV4, Fig. 1A) as a probe. Southern analysis gave two positive bands of 4.2 kb and 2 kb from fosmids 11D6 and 18B4. The 4.2-kb HincII fragment was further subcloned into pUC18 at the S ...
sets of metaphors in multilevel cognitive models
... yazike y tekste", p.119-133); that just the metaphors play the key roles in various cognitive models (Lakoff, Johnson, 1980; MacCormac, 1985). Now, rapidly grows the interest to metaphors in the aspects of theories of cognition, logics, cognitive psychology, linguistics; last years, the research of ...
... yazike y tekste", p.119-133); that just the metaphors play the key roles in various cognitive models (Lakoff, Johnson, 1980; MacCormac, 1985). Now, rapidly grows the interest to metaphors in the aspects of theories of cognition, logics, cognitive psychology, linguistics; last years, the research of ...
Supplemental Table 2. Definition of nine
... Heterozygous mutations as defined in the category III. Patient may be a carrier of such highly-likely disease-causing mutations. Such mutations in heterozygous format may not be disease-causing, but may significantly increase the genetic risk for offspring if both parents carry the same mutations or ...
... Heterozygous mutations as defined in the category III. Patient may be a carrier of such highly-likely disease-causing mutations. Such mutations in heterozygous format may not be disease-causing, but may significantly increase the genetic risk for offspring if both parents carry the same mutations or ...
Name
... the same complement of genes with one member of the pair inherited from the father and the other from the mother. Chromosomes that are homologous are almost always the same size, have their centromeres in the same position and carry the same number and type of genes. (An exception to this rule will ...
... the same complement of genes with one member of the pair inherited from the father and the other from the mother. Chromosomes that are homologous are almost always the same size, have their centromeres in the same position and carry the same number and type of genes. (An exception to this rule will ...
Selection of Functional Signal Peptide Cleavage Sites from a Library of Random Sequences.
... of substitutions as those of mutants from the 1-mg/ml selection. Proline is not found at position - 4 for either of the two mutants that were not selected for ampicillin resistance, and mutant 7-2 is the only mutant in the entire set that does not have an alanine in the region from -4 to +2. Steady- ...
... of substitutions as those of mutants from the 1-mg/ml selection. Proline is not found at position - 4 for either of the two mutants that were not selected for ampicillin resistance, and mutant 7-2 is the only mutant in the entire set that does not have an alanine in the region from -4 to +2. Steady- ...
Rare genomic changes and mitochondrial sequences
... among sea spiders were similar using morphological and molecular analyses, some relationships were supported only by molecular data. Arango proposed that more characters were necessary to clearly delineate the pycnogonid lineages (Arango and Wheeler, 2007; Arango, 2002, 2003). Using genomic-level ch ...
... among sea spiders were similar using morphological and molecular analyses, some relationships were supported only by molecular data. Arango proposed that more characters were necessary to clearly delineate the pycnogonid lineages (Arango and Wheeler, 2007; Arango, 2002, 2003). Using genomic-level ch ...
REVIEW Why Do Bacterial Plasmids Carry Some Genes and Not
... plasmids rather than chromosomes: “plasmidselfish” characters such as conjugation, mobilization, inhibition of bacterial cell division, and plasmid surface exclusion and partitioning, which promote the survival and replication of the plasmid as a unit; and optional characters such as resistance to a ...
... plasmids rather than chromosomes: “plasmidselfish” characters such as conjugation, mobilization, inhibition of bacterial cell division, and plasmid surface exclusion and partitioning, which promote the survival and replication of the plasmid as a unit; and optional characters such as resistance to a ...
The home stretch, a first analysis of the nearly completed genome of
... should refer to the ‘Methods’ section of the Minireview ‘An overview of the genome of Nostoc punctiforme, a multicellular, symbiotic cynobacterium’ in this issue for details of the methodologies used in data processing (Meeks et al. 2001). Assembly of 61 000 reads (∼7.0 × coverage) resulted in 195 c ...
... should refer to the ‘Methods’ section of the Minireview ‘An overview of the genome of Nostoc punctiforme, a multicellular, symbiotic cynobacterium’ in this issue for details of the methodologies used in data processing (Meeks et al. 2001). Assembly of 61 000 reads (∼7.0 × coverage) resulted in 195 c ...
Alus
... result in inherited disorders.However, most Alu insertions act like markers since they may segregate with a diseased allele, but the presence of the Alu does not mean that the person will definitely get the disease. • The first report of Alu-mediated recombination causing a prevalent, inherited pred ...
... result in inherited disorders.However, most Alu insertions act like markers since they may segregate with a diseased allele, but the presence of the Alu does not mean that the person will definitely get the disease. • The first report of Alu-mediated recombination causing a prevalent, inherited pred ...
The molecular epidemiology of iridovirus in Murray cod
... arbitrarily chosen as a limit. If both primers in the pair had E-values of less than 1.0 for the same non-target DNA, they were excluded. Candidate primers for discriminatory assays, C50/C51 and C82/C83, were tested against DNA from MCIV, DGIV-2004, RSIV and EHNV. Primers (M68/ M69 and M151/M152) kn ...
... arbitrarily chosen as a limit. If both primers in the pair had E-values of less than 1.0 for the same non-target DNA, they were excluded. Candidate primers for discriminatory assays, C50/C51 and C82/C83, were tested against DNA from MCIV, DGIV-2004, RSIV and EHNV. Primers (M68/ M69 and M151/M152) kn ...
Genome sequence of Aspergillus luchuensis
... and reliable fermentation in warm latitudes, such as Okinawa and the Kyushu islands. There are approximately 50 awamori brewers in Okinawa who originally used their own strains, but recently, they have employed koji-seed (conidiospores) supplied by companies. The market size of awamori is 13,000 Mye ...
... and reliable fermentation in warm latitudes, such as Okinawa and the Kyushu islands. There are approximately 50 awamori brewers in Okinawa who originally used their own strains, but recently, they have employed koji-seed (conidiospores) supplied by companies. The market size of awamori is 13,000 Mye ...
Nucleotide Sequence of the Osmoregulatory proU Operon of
... FIG. 1. Strategy for DNA sequence determination of proU. The map of insert DNA of pHYD58 (20) is shown, and relevailt restriction sites are marked; a kilobase scale is included. The insert includes 5 kb of DNA clockwise of the BgIII site from the E. coli proU iocus (thin line) and 1 kb of Mu c DNA ( ...
... FIG. 1. Strategy for DNA sequence determination of proU. The map of insert DNA of pHYD58 (20) is shown, and relevailt restriction sites are marked; a kilobase scale is included. The insert includes 5 kb of DNA clockwise of the BgIII site from the E. coli proU iocus (thin line) and 1 kb of Mu c DNA ( ...
No Slide Title - Oklahoma State University
... • Isolates will be identified by – Morphology – Gram-Staining – Genetics ...
... • Isolates will be identified by – Morphology – Gram-Staining – Genetics ...
PDF file
... • QTL-targeted marker selection replaced by genomic selection • New genomic tools required to genotype thousands of animals Create High Density SNP chip ...
... • QTL-targeted marker selection replaced by genomic selection • New genomic tools required to genotype thousands of animals Create High Density SNP chip ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... under aerobic conditions in order to differentiate the lactose fermented bacteriafrom the non-lactose fermented bacteria. Well isolated colonies were selected and cultured on Eosin methylene blue agar (Himedia-India) to detect theE. coli isolates, which produce a green metallic sheen. The isolates w ...
... under aerobic conditions in order to differentiate the lactose fermented bacteriafrom the non-lactose fermented bacteria. Well isolated colonies were selected and cultured on Eosin methylene blue agar (Himedia-India) to detect theE. coli isolates, which produce a green metallic sheen. The isolates w ...
PDF
... complement the homoeotic phenotype of trx1, as well as each others lethality. Genetic analysis suggests that all three alleles abolish most, if not all, of the wildtype function of the trx locus (Ingham, 1981); in addition, there is some evidence that trx3 may have an antimorphic component (unpublis ...
... complement the homoeotic phenotype of trx1, as well as each others lethality. Genetic analysis suggests that all three alleles abolish most, if not all, of the wildtype function of the trx locus (Ingham, 1981); in addition, there is some evidence that trx3 may have an antimorphic component (unpublis ...
genes. Numbers of 6-10 copies per genome have
... The SSU genes and flanking regions in TSSU3-2 and TSSU3-8 were sequenced. Using sequences of other SSU genes, the leader peptide, mature coding regions and introns were located. In Figure 2, the sequence of these two genes is shown and compared to that of a previously sequenced tobacco SSU gene, NtS ...
... The SSU genes and flanking regions in TSSU3-2 and TSSU3-8 were sequenced. Using sequences of other SSU genes, the leader peptide, mature coding regions and introns were located. In Figure 2, the sequence of these two genes is shown and compared to that of a previously sequenced tobacco SSU gene, NtS ...
Creating a Karyotype: A Chromosome Study
... Creating a Karyotype: A Chromosome Study An examination of the chromosomes of a cell under high magnification can give a lot of information about an organism. If the cells are from an unborn human, its sex can be determined before it is born. It can also be determined if the unborn may have certain ...
... Creating a Karyotype: A Chromosome Study An examination of the chromosomes of a cell under high magnification can give a lot of information about an organism. If the cells are from an unborn human, its sex can be determined before it is born. It can also be determined if the unborn may have certain ...
Effective transfer of chromosomes carrying leaf rust resistance
... with resistance to leaf rust. The aim of this study was: (1) to characterize the chromosome composition of the hybrids (BC2F2 to BC2F5) of Ae. tauschii × triticale hybrids; (2) to identify the D-genome DNA markers which are linked with leaf rust resistance in the hybrids; and (3) to evaluate the sta ...
... with resistance to leaf rust. The aim of this study was: (1) to characterize the chromosome composition of the hybrids (BC2F2 to BC2F5) of Ae. tauschii × triticale hybrids; (2) to identify the D-genome DNA markers which are linked with leaf rust resistance in the hybrids; and (3) to evaluate the sta ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.