Ontologies
... What Are Ontologies? "An ontology is an explicit specification of some topic. For our purposes, it is a formal and declarative representation which includes the vocabulary (or names) for referring to the terms in that subject area and the logical statements that describe what the terms are and how ...
... What Are Ontologies? "An ontology is an explicit specification of some topic. For our purposes, it is a formal and declarative representation which includes the vocabulary (or names) for referring to the terms in that subject area and the logical statements that describe what the terms are and how ...
Partnership
... progeny from the viable spores. With a powerful co-dominant genetic screening method with markers covering all chromosomes we now can select complementary parents with any given chromosome set from the starting hybrid. The transgene needed to suppress crossovers is required for only a single meiosis ...
... progeny from the viable spores. With a powerful co-dominant genetic screening method with markers covering all chromosomes we now can select complementary parents with any given chromosome set from the starting hybrid. The transgene needed to suppress crossovers is required for only a single meiosis ...
Upwelling, Downwelling, and El Nino
... The chains are held together by hydrogen bonds between the base pairs and by van der Waals forces between adjacent bases on the same ...
... The chains are held together by hydrogen bonds between the base pairs and by van der Waals forces between adjacent bases on the same ...
Bioinformatics - Sequences and Computers
... first clues about how one can detect genes in DNA sequences. 1. Compare a random with an intergenic sequence. What differences can you observe? 2. Compare the second intergenic sequence with the second random sequence. 3. Are intergenic sequences random? Explain. 4. Are there any differences between ...
... first clues about how one can detect genes in DNA sequences. 1. Compare a random with an intergenic sequence. What differences can you observe? 2. Compare the second intergenic sequence with the second random sequence. 3. Are intergenic sequences random? Explain. 4. Are there any differences between ...
Requirements for Human Medical Genome
... The National Pathology Accreditation Advisory Council (NPAAC) was established in 1979 to advise the Australian, State and Territory governments on matters relating to the accreditation of pathology laboratories. A key role of NPAAC is to develop and maintain pathology quality standards for accredit ...
... The National Pathology Accreditation Advisory Council (NPAAC) was established in 1979 to advise the Australian, State and Territory governments on matters relating to the accreditation of pathology laboratories. A key role of NPAAC is to develop and maintain pathology quality standards for accredit ...
The Role of Mismatch Repair in Bacterial Evolution
... The spread of mutators occurs because they can create or acquire a beneficial mutation (e.g. antibiotic resistance) that gives them advantage over non-adapted bacteria. In an asexual population, the mutator may then spread with the advantageous gene, by a phenomenon called »hitch-hiking« (29) and in ...
... The spread of mutators occurs because they can create or acquire a beneficial mutation (e.g. antibiotic resistance) that gives them advantage over non-adapted bacteria. In an asexual population, the mutator may then spread with the advantageous gene, by a phenomenon called »hitch-hiking« (29) and in ...
Lack of homology between two haloacetate dehalogenase genes
... These enzymes differ in halogen specificity; H-1 acts better on monofluoroacetate than on monochloro- or monobromoacetate, but has little activity against monoiodoacetate. H-2 acts on monochloro-, monobromo-, and monoiodoacetate, but not on monofluoroacetate. The two enzymes have been purified and c ...
... These enzymes differ in halogen specificity; H-1 acts better on monofluoroacetate than on monochloro- or monobromoacetate, but has little activity against monoiodoacetate. H-2 acts on monochloro-, monobromo-, and monoiodoacetate, but not on monofluoroacetate. The two enzymes have been purified and c ...
BlastLecture8
... • To find homologous sequences to your unknown to determine function • To find other related sequences to do evolutionary studies (trees) or to make specialised database (nematode 16sRNA) • To find the mouse or E.coli homolog of your gene of interest • To find genes in a newly sequenced genome • To ...
... • To find homologous sequences to your unknown to determine function • To find other related sequences to do evolutionary studies (trees) or to make specialised database (nematode 16sRNA) • To find the mouse or E.coli homolog of your gene of interest • To find genes in a newly sequenced genome • To ...
1 Single molecule sequencing of THCA synthase reveals
... it could be assumed that the 6 amino acid divergence from the inactive form represents a very weakly active THCAS gene. The cultivar was positive for the Bd:Bd genotype described by de Miejer, and ...
... it could be assumed that the 6 amino acid divergence from the inactive form represents a very weakly active THCAS gene. The cultivar was positive for the Bd:Bd genotype described by de Miejer, and ...
The Complete Genome Sequence of Clostridium aceticum: a
... he anaerobic autotrophic formation of acetate from a CO2-H2 gas mixture was first described in some detail in 1932 (1). Anaerobic sludge was used for those experiments, so that no single organism could be identified that was responsible for this metabolic activity. The first pure culture of such a b ...
... he anaerobic autotrophic formation of acetate from a CO2-H2 gas mixture was first described in some detail in 1932 (1). Anaerobic sludge was used for those experiments, so that no single organism could be identified that was responsible for this metabolic activity. The first pure culture of such a b ...
HiPer® Bacterial Conjugation Teaching Kit
... elements. The factors (plasmids) contain about 20-30 genes, most of which are associated with conjugation. These genes encode enzymes that replicate DNA during conjugation and structural proteins needed to synthesize special pili at the cell surface. Known as F pili or sex pili, these hairlike fibre ...
... elements. The factors (plasmids) contain about 20-30 genes, most of which are associated with conjugation. These genes encode enzymes that replicate DNA during conjugation and structural proteins needed to synthesize special pili at the cell surface. Known as F pili or sex pili, these hairlike fibre ...
An Investigation of Codon Usage Bias Including
... predictive process. For this reason it is important to understand the interplay of multiple biases in an organism’s genome. We present here new techniques in the measurement and analysis of multiple biases in prokaryotic genomic data. Included is a visualization technique aimed at demonstrating geno ...
... predictive process. For this reason it is important to understand the interplay of multiple biases in an organism’s genome. We present here new techniques in the measurement and analysis of multiple biases in prokaryotic genomic data. Included is a visualization technique aimed at demonstrating geno ...
Comparison of DNA Sequences with Protein Sequences
... to join some clearly related superfamilies (W.R.P, manuscript in preparation; the PIR39b database is available for downloading from ftp. virginia.edu:/pub/fasta). Two sequences from each of 46 families of proteins were used for these tests. The cDNA sequences, and their corresponding open reading fr ...
... to join some clearly related superfamilies (W.R.P, manuscript in preparation; the PIR39b database is available for downloading from ftp. virginia.edu:/pub/fasta). Two sequences from each of 46 families of proteins were used for these tests. The cDNA sequences, and their corresponding open reading fr ...
Fighting the good cause: meaning, purpose
... inserts at new sites faster than mutations degrade source DNA. Mutations that enhance transposition disperse to new sites while mutations that reduce transposition accumulate at old sites. An active element must stay one jump ahead of inactivating mutations. ...
... inserts at new sites faster than mutations degrade source DNA. Mutations that enhance transposition disperse to new sites while mutations that reduce transposition accumulate at old sites. An active element must stay one jump ahead of inactivating mutations. ...
pdf
... (GGGGTT in the case of Tetrahymena) and then shifts over to synthesize another repeating unit. If the enzyme dissociates from one telomere after each repeating unit, then its processivity is very low, i.e. 6 nucleotides. If it shifts over on the same telomere, then its processivity is higher. Note t ...
... (GGGGTT in the case of Tetrahymena) and then shifts over to synthesize another repeating unit. If the enzyme dissociates from one telomere after each repeating unit, then its processivity is very low, i.e. 6 nucleotides. If it shifts over on the same telomere, then its processivity is higher. Note t ...
Document
... • Diagnostics for detecting genetic diseases • Gene therapy (e.g. ADA, CF) • Vaccine development (recombinant vaccines) ...
... • Diagnostics for detecting genetic diseases • Gene therapy (e.g. ADA, CF) • Vaccine development (recombinant vaccines) ...
pdf format - Faculty members Homepages
... HDRP was used as a probe. Two transcripts at 9.8 and 4.1 kb were detected in all tissues examined, albeit at different levels (Fig. 2A). The 4.1-kb transcript is shorter than the 4.4-kb HDRP transcript (25). A third transcript at 1.2 kb was detected in placenta (Fig. 2 A). Similarly to HDRP (25), hi ...
... HDRP was used as a probe. Two transcripts at 9.8 and 4.1 kb were detected in all tissues examined, albeit at different levels (Fig. 2A). The 4.1-kb transcript is shorter than the 4.4-kb HDRP transcript (25). A third transcript at 1.2 kb was detected in placenta (Fig. 2 A). Similarly to HDRP (25), hi ...
What is Biotechnology
... • Diagnostics for detecting genetic diseases • Gene therapy (e.g. ADA, CF) • Vaccine development (recombinant vaccines) ...
... • Diagnostics for detecting genetic diseases • Gene therapy (e.g. ADA, CF) • Vaccine development (recombinant vaccines) ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.