S-Phase Checkpoint Genes Safeguard High
... each knockout. Oligonucleotide tag sequences present in yeast sometimes differ from their design sequences, presumably due to synthesis errors. Some tags will therefore exhibit poor hybridization, but most often only the UPTAG or DOWTAG of any given construct is affected. To filter out the effect of ...
... each knockout. Oligonucleotide tag sequences present in yeast sometimes differ from their design sequences, presumably due to synthesis errors. Some tags will therefore exhibit poor hybridization, but most often only the UPTAG or DOWTAG of any given construct is affected. To filter out the effect of ...
Molecular Systematics of the Genus Zoogloea and Emendation of
... Zoogloea, and suggest the utility of rhodoquinone analysis as a new tool for identification of Zoogloea strains. The genus Zoogloea has historically been considered a member of the family Pseudomonadaceae (25, 26), but this placement is tentative and based only on phenotypic information. Phylogeneti ...
... Zoogloea, and suggest the utility of rhodoquinone analysis as a new tool for identification of Zoogloea strains. The genus Zoogloea has historically been considered a member of the family Pseudomonadaceae (25, 26), but this placement is tentative and based only on phenotypic information. Phylogeneti ...
Touring Ensembl: A practical guide to genome browsing Open Access
... The ongoing increase in the number of databases in biological fields provides a large-scale resource. Last year saw the development of nearly 100 new molecular biological databases, bringing the total number of popular databases in this field to over 1,000 [1]. However, different formats and present ...
... The ongoing increase in the number of databases in biological fields provides a large-scale resource. Last year saw the development of nearly 100 new molecular biological databases, bringing the total number of popular databases in this field to over 1,000 [1]. However, different formats and present ...
PDF
... assembly. We also found that more mRNAs had higher percentage matches in Cel2 than in MGSCv3 (that is, > 97%). As a further test, we especially investigated how well long mRNAs can be matched to each assembly. The 10 longest mRNA sequences are all matched well with both assemblies, except for the pi ...
... assembly. We also found that more mRNAs had higher percentage matches in Cel2 than in MGSCv3 (that is, > 97%). As a further test, we especially investigated how well long mRNAs can be matched to each assembly. The 10 longest mRNA sequences are all matched well with both assemblies, except for the pi ...
Chapter_01 1..22 - Wiley-VCH
... first genetic map with 37 markers distributed on 7 linkage groups (summarized in Swiecicki et al. 2000). Large collections of visible markers are today available for several crop species and for Arabidopsis thaliana (Koornneef et al. 1987; Neuffer et al. 1997). In the process of finding more and mor ...
... first genetic map with 37 markers distributed on 7 linkage groups (summarized in Swiecicki et al. 2000). Large collections of visible markers are today available for several crop species and for Arabidopsis thaliana (Koornneef et al. 1987; Neuffer et al. 1997). In the process of finding more and mor ...
The Nucleotide and Derived Amino Acid
... (19) of human intestine mRNA. In each case, the hybridization-selected mRNA directed the synthesis of a M , = 46,000 protein that was immunoprecipitated by an antibody specific for human apo-A-IV (data not shown). The identity of the cloned inserts was subsequently demonstrated by nucleotide sequenc ...
... (19) of human intestine mRNA. In each case, the hybridization-selected mRNA directed the synthesis of a M , = 46,000 protein that was immunoprecipitated by an antibody specific for human apo-A-IV (data not shown). The identity of the cloned inserts was subsequently demonstrated by nucleotide sequenc ...
Evolution of genetic and genomic features unique to the human
... 1990s permitted the evaluation of large-scale structural changes between humans and great apes that were not visible with conventional banding techniques12,13. More recently, these studies were aided by interspecies bacterial artificial chromosome (BAC)-based array-based comparative genomic hybridiz ...
... 1990s permitted the evaluation of large-scale structural changes between humans and great apes that were not visible with conventional banding techniques12,13. More recently, these studies were aided by interspecies bacterial artificial chromosome (BAC)-based array-based comparative genomic hybridiz ...
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic
... kanMX4 (Wach et al. 1994). On the other hand, the pRS series offers an unmatched selection of prototrophic markers for PCR-mediated replacement. Hence, researchers may find themselves employing two or more pairs of oligonucleotides to replace a particular gene sequence with markers from different pla ...
... kanMX4 (Wach et al. 1994). On the other hand, the pRS series offers an unmatched selection of prototrophic markers for PCR-mediated replacement. Hence, researchers may find themselves employing two or more pairs of oligonucleotides to replace a particular gene sequence with markers from different pla ...
Supplementary Notes S1 (doc 64K)
... the range of probe Tm and length represented by the actual region probes extracted above. Initially, ~1.9 million random probes were generated. These probes were subjected to the same quality tests as the experimental probes but were tested to ensure that they have minimal homology to the human geno ...
... the range of probe Tm and length represented by the actual region probes extracted above. Initially, ~1.9 million random probes were generated. These probes were subjected to the same quality tests as the experimental probes but were tested to ensure that they have minimal homology to the human geno ...
Bio Lab Rebop Genetics
... Rebops are ferocious imaginary animals that feed on cute furry puppies. They are prolific and require minimal care. In this activity, you and a partner will perform a genetic cross to make a baby Rebop. After carrying out the genetic cross, you will construct your baby Rebop. In the process, you wil ...
... Rebops are ferocious imaginary animals that feed on cute furry puppies. They are prolific and require minimal care. In this activity, you and a partner will perform a genetic cross to make a baby Rebop. After carrying out the genetic cross, you will construct your baby Rebop. In the process, you wil ...
Bio II Ch 19 Eukaryotic Genomes
... shortens with each round of replication and they bind to proteins that protect the ends of chromosomes from degradation and fusion with other chromosomes. ...
... shortens with each round of replication and they bind to proteins that protect the ends of chromosomes from degradation and fusion with other chromosomes. ...
Reebops
... Reebop activity in their classroom, but feel a little hesitant about their genetics knowledge. The Reebop activity was originally written for middle and high school students. However, many elementary teachers find that they can use Reebops to teach a variety of lessons to younger students. This outl ...
... Reebop activity in their classroom, but feel a little hesitant about their genetics knowledge. The Reebop activity was originally written for middle and high school students. However, many elementary teachers find that they can use Reebops to teach a variety of lessons to younger students. This outl ...
GeneCensus - Gerstein Lab Publications
... Both of these views are linked to additional modules representing more traditional analysis formats. These include modules that examine open reading frames (ORFs), organisms, and various compositions of genomes. In general, it is relatively difficult to integrate disparate information sources into o ...
... Both of these views are linked to additional modules representing more traditional analysis formats. These include modules that examine open reading frames (ORFs), organisms, and various compositions of genomes. In general, it is relatively difficult to integrate disparate information sources into o ...
EC and Genetics - University of Houston
... best possible plant (such as recurrent selection, which will be the topic of this talk). mutation not very important, because it is hard to control; large population sizes are difficult to handle because of pragmatic reasons. ...
... best possible plant (such as recurrent selection, which will be the topic of this talk). mutation not very important, because it is hard to control; large population sizes are difficult to handle because of pragmatic reasons. ...
Screening of RYR1 genotypes in swine population by a rapid and
... assay design are crucial points that can increase the amplitude of the profile difference and make sequence discrimination easier. However, the primers used for HRM must generate short amplicons. According to the manufacturer's recommendation the best results can be obtained with amplicons up to 300 ...
... assay design are crucial points that can increase the amplitude of the profile difference and make sequence discrimination easier. However, the primers used for HRM must generate short amplicons. According to the manufacturer's recommendation the best results can be obtained with amplicons up to 300 ...
Meiosis
... Meiosis II – Division of sister chromatids Telophase II : -nuclear membrane reforms -chromatin forms -cytokinesis produces 4 haploid (n) cells ...
... Meiosis II – Division of sister chromatids Telophase II : -nuclear membrane reforms -chromatin forms -cytokinesis produces 4 haploid (n) cells ...
Fully automated pipeline for detection of sex linked genes using
... principle, they contain all information with regard to how a sample differs from the reference sequence. SAMtools parameters have been chosen by rule of thumb after extensive testing. It must be taken into account that reporting many variants that will contain sequencing errors or misalignments can ...
... principle, they contain all information with regard to how a sample differs from the reference sequence. SAMtools parameters have been chosen by rule of thumb after extensive testing. It must be taken into account that reporting many variants that will contain sequencing errors or misalignments can ...
Bacterial plasmids - Micro-Rao
... Plasmids are defined as double stranded, extrachromosomal genetic elements that replicate independently of the host cell chromosome and are stably inherited. Plasmids capable of integration into the chromosome were earlier called episomes. Plasmids differ from chromosomes in being small and coding f ...
... Plasmids are defined as double stranded, extrachromosomal genetic elements that replicate independently of the host cell chromosome and are stably inherited. Plasmids capable of integration into the chromosome were earlier called episomes. Plasmids differ from chromosomes in being small and coding f ...
A Large Pseudoautosomal Region on the Sex Chromosomes of the
... DM-W and was discovered in the African clawed frog Xenopus laevis (Yoshimoto et al. 2008). DM-W is female specific and originated after divergence from the sister genus Silurana but before diversification of most or all extant species of Xenopus (Bewick et al. 2011). Because Silurana tropicalis (als ...
... DM-W and was discovered in the African clawed frog Xenopus laevis (Yoshimoto et al. 2008). DM-W is female specific and originated after divergence from the sister genus Silurana but before diversification of most or all extant species of Xenopus (Bewick et al. 2011). Because Silurana tropicalis (als ...
... Strains. The yeast strains used in this study are four S. cerevisiae ⫻ S. kudriavzevii hybrids. W27 and W46 were isolated from pinot noir must fermentations in Jenins and Stäfa, Switzerland, respectively. They correspond to commercial wine yeasts (Lallemand, Inc., Montreal, Canada). SPG16-91 and 44 ...
Expression and purification of four different rhizobial acyl carrier
... GTC GAC GCC AAG ATG ATC-3h ; restriction sites are underlined and respective enzymes are given before the sequence) were designed from the internal sequenced region (67 bp) of the acpP gene cloned in pTB5013 (Fig. 2a) in such a way that they annealed to the target DNA template 18 bp apart and their ...
... GTC GAC GCC AAG ATG ATC-3h ; restriction sites are underlined and respective enzymes are given before the sequence) were designed from the internal sequenced region (67 bp) of the acpP gene cloned in pTB5013 (Fig. 2a) in such a way that they annealed to the target DNA template 18 bp apart and their ...
Disrupting antibiotic resistance propagation by inhibiting
... horizontal gene transfers within and between bacterial strains (reviewed in ref. 1), as first described for the Escherichia coli F plasmid by Lederberg and Tatum in 1946 (2). Conjugative DNA transfer is also the central mechanism by which antibiotic resistance and virulence factors are propagated in ...
... horizontal gene transfers within and between bacterial strains (reviewed in ref. 1), as first described for the Escherichia coli F plasmid by Lederberg and Tatum in 1946 (2). Conjugative DNA transfer is also the central mechanism by which antibiotic resistance and virulence factors are propagated in ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.