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Press Release - Max Delbrück Center for Molecular Medicine
Press Release - Max Delbrück Center for Molecular Medicine

... than current high-throughput technologies. The researchers of the BIMSB will use this thirdgeneration sequencing technology, which was launched on the market in April 2011 by Pacific Biosciences, Menlo Park, California, USA, to gain deeper insight into gene regulation. The new sequencer, PacBio RS, ...
CH-13 Sect 1
CH-13 Sect 1

... 15. Biologists use ____________________________ to cut DNA molecules at a specific sequence of nucleotides to make smaller fragments. 16. Circle the letter of the process by which DNA fragments are separated and analyzed. a. gel electrophoresis b. extraction c. transformation ...
Prescott`s Microbiology, 9th Edition Chapter 19 –Microbial
Prescott`s Microbiology, 9th Edition Chapter 19 –Microbial

... Unsure student understand that melting means the hydrogen bonds and hydrophobic base stacking interactions between strands are disrupted. The covalent bonds connecting nucleotides within each strand are not affected, thus melting is reversible. GC rich DNA is more stable than AT rich, thus as the GC ...
glossary of technical terms
glossary of technical terms

... chromosomes of almost all organisms, made up of four different kinds of bases, which are abbreviated A, C, T and G. A DNA fragment that is ten bases long might have a base sequence of, for example, ATCGTTCCTG. The particular sequence of bases encodes important information in an individual’s genetic ...
Human Genome
Human Genome

... Page 403 ...
PCR analysis
PCR analysis

... Introns often vary in their size and sequence among individuals, while exons do not. This variation is thought to be the result of the accumulation of different mutations in DNA throughout evolution. These mutations in our noncoding DNA are silently passed on to our descendants; we do not notice th ...
“Bill Nye: Genes” Video Worksheet
“Bill Nye: Genes” Video Worksheet

... 6. How many times longer is DNA than it is wide? About 200 km 7. How does Bill define a Gene? A specific piece of DNA 8. Why is the white blood cell dark on the computer screen? Because it has ...
Communication_files/Bad Presentation #3
Communication_files/Bad Presentation #3

... • ~500 Pathogens occur in livestock • 40% are zoonotic • Pathogens in dogs are more than 70% zoonotic ...
Gene Q
Gene Q

... following experiment. Four pairs of ...
Slide ()
Slide ()

How hereditary information is stored in the genome.
How hereditary information is stored in the genome.

ModernGeneticsII
ModernGeneticsII

... 2. A scientist decided to clone a human being. He obtained an egg cell from his wife and removed the nucleus, while at the same time, obtained a liver cell nucleus from his daughter. He inserted the liver cell nucleus into the egg cell and provided it with the proper hormones to develop into an embr ...
the element makes na RNA copy of itself which is reversed
the element makes na RNA copy of itself which is reversed

... Rec A has multiple DNA binding sites, hence can hold a single strand and a double helix together Rec A is also a DNA-dependent ATPase ...
Zoo/Bot 3333
Zoo/Bot 3333

... produce compatible ends; c) Only BamHI and BglII fragments are compatible; d) Only BamHI and XbaI fragments are compatible; e) only BglII and XbaI fragments are compatible. ...
Document
Document

References - Proceedings of the Royal Society B
References - Proceedings of the Royal Society B

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Slide ()

Mapping Life
Mapping Life

... Mapping Life (Genomics) Genomics is the use of the information collected in The Human Genome Project and similar projects for other organisms. Once the sequence of DNA that makes a gene is known, the information can be used to repair problems or improve the organism. Plant genes can be changed to ma ...
Nerve activates contraction
Nerve activates contraction

... to transfer the DNA fragments from the gel to a sheet of nitrocellulose paper, still separated by size. • This also denatures the DNA fragments. • Bathing this sheet in a solution containing our probe allows the probe to attach by base-pairing (hybridize) to the DNA sequence of interest and we can v ...
ppt
ppt

... these genes are responsible for a useful characteristic displayed by the host bacterium. For example, the ability to survive in normally toxic concentrations of antibiotics such as ampicillin, chloramphenicol or tetracycline is often due to the presence in the bacterium of a plasmid carrying antibio ...
Presentation - College of American Pathologists
Presentation - College of American Pathologists

... Dot blot/Reverse dot blot Polymerase chain reaction SSCP/DGGE RT-PCR DNA sequencing TaqMan, real-time PCR Invader assay In situ hybridization Microarray hybridization High-density microarray hybridization Array comparative genomic hybridization Whole-genome sequencing ...
4.4 Genetic Engineering and Biotechnology
4.4 Genetic Engineering and Biotechnology

... Outline a basic technique used for gene transfer involving plasmids, a host cell (bacterium, yeast or other cell), restriction enzymes (endonucleases) and DNA ligase.  The use of E. coli in gene technology is well documented.  Most of its DNA is in one circular chromosome, but it also has plasmids ...
Answers to Biological Inquiry Questions – Brooker et al ARIS site
Answers to Biological Inquiry Questions – Brooker et al ARIS site

... ANSWER: Retroelements. A single element can be transcribed into multiple copies of RNA, which can be converted to DNA by reverse transcriptase, and inserted into multiple sites in the genome. Figure 21.8 BIOLOGICAL INQUIRY QUESTION: What is the advantage of a gene family? ANSWER: The overall advanta ...
human oct-1 gene located on chromosome 1
human oct-1 gene located on chromosome 1

... Investigations of cellular proteins able to interact with the octamer consensus sequence (ATGCAAAT), which is found as a controlling element in a number of disparate gene systems, has identified a complex set of factors with distinct expression patterns. The largest of these proteins is a generally ...
DNA - VanityWolveriine
DNA - VanityWolveriine

... genetic characteristics in all life forms, constructed of two nucleotide strands coiled around each other in a ladder like arrangement with the sidepieces composed of alternating phosphate and deoxyribose units and the rungs composed of the perinea and pyrimidine bases adenine, guanine, cytosine, an ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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