Lecture 2 PSY391S John Yeomans
... • Blotting to find specific chains: SouthernDNA, Northern-RNA, Western-proteins. • In situ hybridization: Find RNA in brain. • Immunocytochemistry: Find proteins in ...
... • Blotting to find specific chains: SouthernDNA, Northern-RNA, Western-proteins. • In situ hybridization: Find RNA in brain. • Immunocytochemistry: Find proteins in ...
Quiz Review: Chapter 11: Eukaryotic Genome Organization Chapter
... repetitive sequences include HETEROCHROMATIN and CENTROMERIC DNA. Middle repetitive sequences appear in the genome at regular intervals and are useful tools in genomic testing. Examples include Variable Number Tandem Repeats (VNTR’s), SINE’s and LINE’s. What is critically important about CENTROMERIC ...
... repetitive sequences include HETEROCHROMATIN and CENTROMERIC DNA. Middle repetitive sequences appear in the genome at regular intervals and are useful tools in genomic testing. Examples include Variable Number Tandem Repeats (VNTR’s), SINE’s and LINE’s. What is critically important about CENTROMERIC ...
FAQs (frequently asked questions) Q.1 What are plasmids? Ans
... PstI, PvuI and Sca l lie within the β-lactamase gene. Cloning of a DNA fragment into any of these 11 sites results in the insertional inactivation of either one of the antibiotic resistance markers. ...
... PstI, PvuI and Sca l lie within the β-lactamase gene. Cloning of a DNA fragment into any of these 11 sites results in the insertional inactivation of either one of the antibiotic resistance markers. ...
Slide 1
... (e.g. genes, but wait till next slides) are inherited together. Two markers located on the same chromosome can be separated only through the process of recombination. If they are separated, childs will have just one marker from the pair. However, the closer the markers are each to other, the more ti ...
... (e.g. genes, but wait till next slides) are inherited together. Two markers located on the same chromosome can be separated only through the process of recombination. If they are separated, childs will have just one marker from the pair. However, the closer the markers are each to other, the more ti ...
Unit 4 Review Sheet Genetics and Biotechnology Vocabulary
... - Do you know how to use the codon chart? - Why is the sequence of amino acids important to the shape and function of a protein? *You do NOT need to know the names of the enzymes involved in this process. Mutations - What is a mutation? - What kind of mutations can happen to DNA (i.e. a nucleotide i ...
... - Do you know how to use the codon chart? - Why is the sequence of amino acids important to the shape and function of a protein? *You do NOT need to know the names of the enzymes involved in this process. Mutations - What is a mutation? - What kind of mutations can happen to DNA (i.e. a nucleotide i ...
Slide 1
... new cotton community database to further enable basic, translational and applied cotton research. ...
... new cotton community database to further enable basic, translational and applied cotton research. ...
Document
... is higher after drug treatment Red -- expression of the gene is lower after drug treatment ...
... is higher after drug treatment Red -- expression of the gene is lower after drug treatment ...
Principles_of_Genetic_engineering
... Using Reverse transcriptase • Gene for this enzyme originally found in retroviruses (contain RNA instead of DNA) • why is the enzyme useful for these? • Converts mRNA into single-stranded cDNA – E.g. insulin mRNA from B-cells of islets of Langerhans • Then DNA polymerase produces complementary stran ...
... Using Reverse transcriptase • Gene for this enzyme originally found in retroviruses (contain RNA instead of DNA) • why is the enzyme useful for these? • Converts mRNA into single-stranded cDNA – E.g. insulin mRNA from B-cells of islets of Langerhans • Then DNA polymerase produces complementary stran ...
11/01 Molecular genetic analysis and biotechnology
... • Cloning vector: a replicating DNA molecule attached with a foreign DNA fragment to be introduced into a cell – Has features that make it easier to insert DNA and select for presence of vector in cell. • Origin of replication • Antibiotic resistance gene • Cloning site ...
... • Cloning vector: a replicating DNA molecule attached with a foreign DNA fragment to be introduced into a cell – Has features that make it easier to insert DNA and select for presence of vector in cell. • Origin of replication • Antibiotic resistance gene • Cloning site ...
Document
... • eg. bacterial, P1 bacteriophage- derived, mammalian, and yeast artificial chromosomes [ BACs, PACs,MACs and YACs, respectively]) ...
... • eg. bacterial, P1 bacteriophage- derived, mammalian, and yeast artificial chromosomes [ BACs, PACs,MACs and YACs, respectively]) ...
Document
... images of electric molecules injected themselves somewhere between the mountain road and my eyes." Opening words, Dancing Naked in the Mind Field, © 1998, by Dr. Kary Mullis, Pantheon Books. ...
... images of electric molecules injected themselves somewhere between the mountain road and my eyes." Opening words, Dancing Naked in the Mind Field, © 1998, by Dr. Kary Mullis, Pantheon Books. ...
A.D.Hershey and Martha Chase (1952). Independent Function of
... heredity material. But, since chromosomes are made of both protein and DNA, the question still remained as to which of the two conferred heritable information. 1928- Frederick Griffith - showed that heat killed virulent bacteria can transform a non-virulent strain. 1944- Avery, MacLeod, McCarty - re ...
... heredity material. But, since chromosomes are made of both protein and DNA, the question still remained as to which of the two conferred heritable information. 1928- Frederick Griffith - showed that heat killed virulent bacteria can transform a non-virulent strain. 1944- Avery, MacLeod, McCarty - re ...
CSCE590/822 Data Mining Principles and Applications
... DNA, Chromosome, Genome Sequence models Sequence database, retrieval Whole genome sequence analysis ...
... DNA, Chromosome, Genome Sequence models Sequence database, retrieval Whole genome sequence analysis ...
Sc9 - a 3.1(teacher notes)
... These variations in forms are called alleles. The ultimate combination of the chromosome pair is what makes the variation possible - combining the different variations of different characteristics to create a unique variation. ...
... These variations in forms are called alleles. The ultimate combination of the chromosome pair is what makes the variation possible - combining the different variations of different characteristics to create a unique variation. ...
Unit 4 Genetics - Jamestown Public Schools
... - If it occurs, abnormal ____ of ________________ could find their way into ____________, & a _____________ of ___________________ #’s may result - ________ Syndrome results when there is an _________ on chromosome ___ ...
... - If it occurs, abnormal ____ of ________________ could find their way into ____________, & a _____________ of ___________________ #’s may result - ________ Syndrome results when there is an _________ on chromosome ___ ...
Document
... Using dye-labeled nucleotides, scientists can stop replication at any point along a single DNA strand. The fragments can then be separated by size using gel electrophoresis and “read,” base-bybase. ...
... Using dye-labeled nucleotides, scientists can stop replication at any point along a single DNA strand. The fragments can then be separated by size using gel electrophoresis and “read,” base-bybase. ...
Chapter 20: DNA Technology & Genomics
... Copies of a gene for research Produce Protein product ...
... Copies of a gene for research Produce Protein product ...
Study Guide 3 Bio 4 C
... Ch. 20 DNA Technology genetic engineering, recombinant DNA, biotechnology, restriction enzymes (endonuclease), restriction fragments, gene therapy, DNA ligase, gel electrophoresis, what is PCR and how is it used?, RFLP, applications of RFLP, forensic uses of DNA technology, DNA fingerprinting, agric ...
... Ch. 20 DNA Technology genetic engineering, recombinant DNA, biotechnology, restriction enzymes (endonuclease), restriction fragments, gene therapy, DNA ligase, gel electrophoresis, what is PCR and how is it used?, RFLP, applications of RFLP, forensic uses of DNA technology, DNA fingerprinting, agric ...
Gene Cloning
... Prepare gene of interest mRNA to DNA Restriction enzymes to cut DNA plus plasmid Marker genes, antibiotic resistance and lac-c are added DNA ligase to attach isolated DNA with plasmid Insertion back into host bacterium (transformation) recombinant DNA Replication Screening is then carried o ...
... Prepare gene of interest mRNA to DNA Restriction enzymes to cut DNA plus plasmid Marker genes, antibiotic resistance and lac-c are added DNA ligase to attach isolated DNA with plasmid Insertion back into host bacterium (transformation) recombinant DNA Replication Screening is then carried o ...
No Slide Title
... • Single stranded T-DNA fragment is converted to dsDNA fragment by plant cell ...
... • Single stranded T-DNA fragment is converted to dsDNA fragment by plant cell ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.