Gene Tagging with Transposons
... Transposase moves the element by creating a staggered cut at either end in a random spot of the genome ...
... Transposase moves the element by creating a staggered cut at either end in a random spot of the genome ...
Lecture #9 Date - Biology Junction
... recognition sequence for a particular restriction enzyme Restriction fragments: segments of DNA cut by restriction enzymes in a reproducable way Sticky end: short extensions of restriction fragments DNA ligase: enzyme that can join the sticky ends of DNA fragments Cloning vector: DNA molecule that c ...
... recognition sequence for a particular restriction enzyme Restriction fragments: segments of DNA cut by restriction enzymes in a reproducable way Sticky end: short extensions of restriction fragments DNA ligase: enzyme that can join the sticky ends of DNA fragments Cloning vector: DNA molecule that c ...
Genetic Technology
... Add in gene of interest (plasmid is now recombinant DNA molecule) Put back into bacteria Many reproductive cycles later = amplification of gene & protein it makes ...
... Add in gene of interest (plasmid is now recombinant DNA molecule) Put back into bacteria Many reproductive cycles later = amplification of gene & protein it makes ...
The Genetic Code
... • The sides of the ‘ladder’ are made of a sugar molecule, deoxyribose, alternating with a phosphate molecule. ...
... • The sides of the ‘ladder’ are made of a sugar molecule, deoxyribose, alternating with a phosphate molecule. ...
workshop-1
... - coding and non-coding sequence are slightly different in composition - some ‘possible’ splice sites are more likely than others scan genomic sequence … . . .CGTCGTATGGCTTCGATGTAGTACATCGGATCGGTATGGAATCATTTCAGTCGCTAGCTAGCCTAACGTATATAGCTAGGTAAGACTA. . ...
... - coding and non-coding sequence are slightly different in composition - some ‘possible’ splice sites are more likely than others scan genomic sequence … . . .CGTCGTATGGCTTCGATGTAGTACATCGGATCGGTATGGAATCATTTCAGTCGCTAGCTAGCCTAACGTATATAGCTAGGTAAGACTA. . ...
Emergent Properties of Reduced-Genome
... These specific deletions seem useful for producing stable biological agents Greater efficiency in plasmid delivery ...
... These specific deletions seem useful for producing stable biological agents Greater efficiency in plasmid delivery ...
What is DNA?
... process of Mitosis. • Replication is the process by which DNA duplicate in order to form two identical cells • Cells need to reproduce to create new cells for growth, repair of tissue, (healing) and to produce other things our body needs. • Why do the body cells have to be identical? ...
... process of Mitosis. • Replication is the process by which DNA duplicate in order to form two identical cells • Cells need to reproduce to create new cells for growth, repair of tissue, (healing) and to produce other things our body needs. • Why do the body cells have to be identical? ...
Examination in Gene Technology, TFKE38 2011-10-18
... 4) You want to study the ribosomal proteins and has therefore chosen to clone protein L21. The figure below (figure 2) shows the DNA sequence and protein sequence of L21. NOTE: This question provides a total of 20 points a) Construct primers to find the gene in a cDNA library (2p) b) In order to qu ...
... 4) You want to study the ribosomal proteins and has therefore chosen to clone protein L21. The figure below (figure 2) shows the DNA sequence and protein sequence of L21. NOTE: This question provides a total of 20 points a) Construct primers to find the gene in a cDNA library (2p) b) In order to qu ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 /9.00-12.00
... VI. Answer the following in detail, not morebthan 1500 words each ...
... VI. Answer the following in detail, not morebthan 1500 words each ...
Ligation and Transformation
... growth and replication • Our host cells: HB101 E. coli • Our foreign DNA: GFP & b-lactamase genes (contained in the pGLO plasmid) ...
... growth and replication • Our host cells: HB101 E. coli • Our foreign DNA: GFP & b-lactamase genes (contained in the pGLO plasmid) ...
Slide 1
... Restriction enzymes cleave specific DNA sequences, many of them produce ‘sticky ends” ...
... Restriction enzymes cleave specific DNA sequences, many of them produce ‘sticky ends” ...
Applied Genetics
... organism with the DNA of another organism. • Recombinant DNA technology was first used in the 1970’s with bacteria. ...
... organism with the DNA of another organism. • Recombinant DNA technology was first used in the 1970’s with bacteria. ...
Lecture 6 pdf - Institute for Behavioral Genetics
... restriction enzymes - cut DNA at specific points in the sequence a point mutation may change the restriction point sequence – DNA will not be cut - DNA fragments of different sizes will be detected ...
... restriction enzymes - cut DNA at specific points in the sequence a point mutation may change the restriction point sequence – DNA will not be cut - DNA fragments of different sizes will be detected ...
Lecture 1, Part I
... • The various genome projects have yielded the complete DNA sequences of many organisms. E.g. human, mouse, yeast, fruitfly, etc. Human: 3 billion base-pairs, 30-40 thousand genes. ...
... • The various genome projects have yielded the complete DNA sequences of many organisms. E.g. human, mouse, yeast, fruitfly, etc. Human: 3 billion base-pairs, 30-40 thousand genes. ...
2nd Semester Review The second semester test covers Meiosis
... 2nd Semester Review The second semester test covers Meiosis, DNA, DNA Technologies, Genetics, Evolution, Basics of Physiology: Digestive System, Circulatory System and Respiratory System, and Ecology. This list will help you prepare. You should also look over all the review documents that you have i ...
... 2nd Semester Review The second semester test covers Meiosis, DNA, DNA Technologies, Genetics, Evolution, Basics of Physiology: Digestive System, Circulatory System and Respiratory System, and Ecology. This list will help you prepare. You should also look over all the review documents that you have i ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.