• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Ch 27 bacteria intro..
Ch 27 bacteria intro..

... 11. How does binding of the trp corepressor and the lac inducer to their respective repressor proteins alter repressor function and transcription in each case? ...
The wrong file for Lecture 8 was posted on the website. I`ve sent the
The wrong file for Lecture 8 was posted on the website. I`ve sent the

... number of times and are usually fairly short sequences (10-25 NT). Moderately repetitive sequences are usually tandem repeats or interspersed sequences that are longer than highly repetitive sequences. ...
Chapter 13: Genetic Technology
Chapter 13: Genetic Technology

... 3. Separating DNA  Gel Electrophoresis – a technique for separating DNA  DNA is negatively charged  Load DNA into gel  Apply electric voltage to gel  DNA will move through gel to positive  Smaller DNA fragments move faster and further  How do you end up with different size fragments that are ...
gabi - beet: the german sugar beet genome - assbt
gabi - beet: the german sugar beet genome - assbt

pGLO lab - Fog.ccsf.edu
pGLO lab - Fog.ccsf.edu

... Figure 21.14 (1 of 2) ...
Biology 207 Workshop 9
Biology 207 Workshop 9

... following diagram. Sizes between each restriction site is given in kilobases (kb). ...
Titan Tutoring for Biology
Titan Tutoring for Biology

... How is a transgenic organism created?  DNA is obtained from organism #1 (donor).  It is cut using restriction enzymes.  This allows a specific gene to be obtained.  The same restriction enzyme is used to cut the DNA of organism #2.  This allows for the production of sticky ends, and the gene fr ...
The Reduced Genome of the Parasitic Microsporidian
The Reduced Genome of the Parasitic Microsporidian

... resequencing of the pathogenic microsporidiian, Enterocytozoon bieneusi, which shows that this parasite has crossed this line and abandoned complete pathways for the most basic carbon metabolism. Comparing two genome sequence surveys of E. bieneusi to genomic data from four other microsporidia revea ...
Chromosome “theory” of inheritance
Chromosome “theory” of inheritance

... MCB140 01-26-07 10 ...
Lecture Slides - METU Computer Engineering
Lecture Slides - METU Computer Engineering

... terms of molecules (in the sense of physicalchemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with these molecules, on a large-scale. • Bioinformatics is a practical discipli ...
Lecture Slides
Lecture Slides

... terms of molecules (in the sense of physicalchemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with these molecules, on a large-scale. • Bioinformatics is a practical discipli ...
This would be given at the end of the unit
This would be given at the end of the unit

... 9. On an electrophoresis gel, band B is closer to the positive end of the gel than is band A. Which of the following statements is true? a. Band B is more negatively charged than band A. b. Band B moved faster than band A. c. Band A is smaller than band B. d. Band B consists of larger DNA fragments ...
Generation and Analysis of AFLP Data
Generation and Analysis of AFLP Data

... require previous sequence information so that PCR primers can be designed – AFLPs and RAPDs allow genetic fingerprinting when previous sequence knowledge is not available ...
Biological Diversity Section 3 Student Notes
Biological Diversity Section 3 Student Notes

... 3.1 DNA - Transmitter of Genetic Code In most organisms, DNA is found in the nucleus of the cell and it is highly organized. An encyclopedia analogy is useful when describing how DNA is ...
HW7 key - WordPress.com
HW7 key - WordPress.com

... (b) Suppose that the human population is 10 billion, and each gene has 10 equally likely variants. How many genes must be examined to identify a person by giving a pattern ...
CB - Human Genome WS 2pp
CB - Human Genome WS 2pp

... Using information from the chapter, complete the compare/contrast table below to compare different types of genetic disorders. If there is not enough room in the table to write your answers, write them on a separate sheet of paper. Type of Disorder ...
Intro to DNA and Genetics
Intro to DNA and Genetics

... Mendel thought that SOMETHING determined how _________________________, and ____________, but did not discover what exactly it was… DNA itself was discovered(?) by __________________ and _____________in ________. They won the ______________________ for this. Although, it is rumored that fellow scien ...
Transposons
Transposons

... transcriptase into cDNA the cDNA integrates into the genome Retroelements are found in all eukaryotes such as Tos in rice, copia in animals Ty1 in yeast ...
Spring Semester Exam Study Guide- Biology 2016 Complete this
Spring Semester Exam Study Guide- Biology 2016 Complete this

... Evidence that considers homologous structures, vestigial organs and embryological development of organisms and how these may be linked to a common ancestor. Evidence that shows how organisms living widely apart may be similar because they shared a common ancestor. ...
Unit 4 Objectives
Unit 4 Objectives

... How the simple structure of DNA lead to such incredible diversity of living things?  How do your genes determine your traits?  What is a gene and what do they do?  How does the information from DNA get converted into traits? ...
(lectures 24
(lectures 24

... doubled twice in the ancestral lineage, and salmonid fishes are all doubled compared to their relatives) . This may be owing to problems with sex-determination in XXYY individuals and owing to not being able to self-fertilize. 29. There are thought to have been at least two genome doublings in the l ...
Go Enrichment analysis using goseq 2014
Go Enrichment analysis using goseq 2014

... For GO enrichment, we take the following things into account: A. Total number of genes we are looking at. B. Number of genes of interest, that is, in our DEG list. C. Total number of genes in the GO term D. Number of genes from our genes of interest that are also in the GO term. If the number of gen ...
Forensic DNA Testing Terminology ABI 310 Genetic Analyzer – a
Forensic DNA Testing Terminology ABI 310 Genetic Analyzer – a

... Identifiler – PCR Amplification Kit (AmpFLSTR® Identifiler™) that provides human identification laboratories with the ability to generate information on 15 STR loci and Amelogenin. In vitro – outside a living organism. Kilobase (kb) – unit of length for DNA fragments equal to 1000 nucleotides. Kinsh ...
DNA Technology
DNA Technology

... What are the practical applications of gel electrophoresis?  Conservation biologists use DNA to determine genetic similarity among populations and individuals  Taxonomists use DNA to help distinguish species  Evolutionary biologists use DNA profiles to compare similarities and differences to con ...
Application of Molecular Biotechnologies to Remediation
Application of Molecular Biotechnologies to Remediation

... Functional PCR: Several PCR-based analyses using amplified catabolic genes; indirect functional assay Direct cloning and sequencing: Direct sequencing of isolated and cloned fragments ...
< 1 ... 367 368 369 370 371 372 373 374 375 ... 561 >

Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report