Production of acylated homoserine lactone by a novel marine strain
... A marine strain of Proteus vulgaris capable of activating multiple acylated homoserine lactone (AHL)-based reporter cultures was isolated. The cognate signal molecule was characterized as octanoyl homoserine lactone (OHL) and its production was observed to be growth dependent, with maximum productio ...
... A marine strain of Proteus vulgaris capable of activating multiple acylated homoserine lactone (AHL)-based reporter cultures was isolated. The cognate signal molecule was characterized as octanoyl homoserine lactone (OHL) and its production was observed to be growth dependent, with maximum productio ...
3 Amino Acids - Minificciones
... We now turn to amino acids having positively charged side chains that render these amino acids highly hydrophilic (Figure 3.5). Lysine and arginine have relatively long side chains that terminate with groups that are positively charged at neutral pH. Lysine is topped by an amino group and arginine b ...
... We now turn to amino acids having positively charged side chains that render these amino acids highly hydrophilic (Figure 3.5). Lysine and arginine have relatively long side chains that terminate with groups that are positively charged at neutral pH. Lysine is topped by an amino group and arginine b ...
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... the fibrous components of the extracellular matrix. Growth factors and cytokines expressed and released by foam cells, smooth muscle cells, and the endothelium itself promote lesion development to a more advanced state, the atheroma. Foam cells and lipids continue to accumulate in the core of the a ...
... the fibrous components of the extracellular matrix. Growth factors and cytokines expressed and released by foam cells, smooth muscle cells, and the endothelium itself promote lesion development to a more advanced state, the atheroma. Foam cells and lipids continue to accumulate in the core of the a ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... A novel compound, the fatty acid synthase (FAS) inhibitor C75, has been proposed to pharmacologically regulate CPT I activity (Price, 2001). Structurally, C75 is a cell-permeable α-methylene-γ-butyrolactone designed to be less reactive and potentially safer than cerulenin, a natural product obtained ...
... A novel compound, the fatty acid synthase (FAS) inhibitor C75, has been proposed to pharmacologically regulate CPT I activity (Price, 2001). Structurally, C75 is a cell-permeable α-methylene-γ-butyrolactone designed to be less reactive and potentially safer than cerulenin, a natural product obtained ...
Iron mediated methylthiolation of tRNA as a regulator of operon
... using isopropanol-HCl (system 'a') (8) . S-nucleotldes from digests of S-tRNA were characterised by electrophoresis at pH 3.5 ( Whatman 3MM paper (8)). Ap,Cp,Gp,Up and s Up (PL Biochemicals Inc.,USA) were used as markers. Radioactive compounds were obtained from The Radiochemical Centre, Amershara. ...
... using isopropanol-HCl (system 'a') (8) . S-nucleotldes from digests of S-tRNA were characterised by electrophoresis at pH 3.5 ( Whatman 3MM paper (8)). Ap,Cp,Gp,Up and s Up (PL Biochemicals Inc.,USA) were used as markers. Radioactive compounds were obtained from The Radiochemical Centre, Amershara. ...
The Enolase Superfamily: A General Strategy for Enzyme
... enzyme family and may allow identification of active site residues. The availability of increasing numbers of threedimensional structures now provides an opportunity to expand families defined on the basis of conserved catalytic properties to include enzymes that catalyze different overall reactions ...
... enzyme family and may allow identification of active site residues. The availability of increasing numbers of threedimensional structures now provides an opportunity to expand families defined on the basis of conserved catalytic properties to include enzymes that catalyze different overall reactions ...
Data mining for important amino acid residues in multiple sequence
... as the main chain or the backbone. The sequence in which residues appear in a main chain is called primary structure. Determined by the primary structure and the local environment, amino acid chains will fold to energetically favorable secondary structures like the extremely regular local sub-struct ...
... as the main chain or the backbone. The sequence in which residues appear in a main chain is called primary structure. Determined by the primary structure and the local environment, amino acid chains will fold to energetically favorable secondary structures like the extremely regular local sub-struct ...
Module 2 Biochemical bases of proliferation, intercellular
... 64. Who synthesized uric acid artificially from glycine and urea in 1882: A. German scientist Veller B. German scientist Fisher C. Polish-Russian scientist Nenskiy D. Austrian physiologist Maresh E. * Ukrainian scientist I. Horbachevskiy 65. Why constant excessive consumption of meat and glandular t ...
... 64. Who synthesized uric acid artificially from glycine and urea in 1882: A. German scientist Veller B. German scientist Fisher C. Polish-Russian scientist Nenskiy D. Austrian physiologist Maresh E. * Ukrainian scientist I. Horbachevskiy 65. Why constant excessive consumption of meat and glandular t ...
Understanding nature`s catalytic toolkit
... [10,11] and water molecules [12] to aid catalysis. However, a source of catalytic power that does not require additional groups stems from the ability of catalytic residues to interact with each other and thus to affect each other’s chemical properties [13]. An early example of this phenomenon was o ...
... [10,11] and water molecules [12] to aid catalysis. However, a source of catalytic power that does not require additional groups stems from the ability of catalytic residues to interact with each other and thus to affect each other’s chemical properties [13]. An early example of this phenomenon was o ...
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in
... diagnostic arena due to a number of factors. One is the enzyme’s limited stability under the prevalent conditions, including elevated temperatures and in the presence of hydrogen peroxide. We are using methods of directed evolution (Morawski et al., 2000; Cherry et al., 1999; Petrounia and Arnold, 2 ...
... diagnostic arena due to a number of factors. One is the enzyme’s limited stability under the prevalent conditions, including elevated temperatures and in the presence of hydrogen peroxide. We are using methods of directed evolution (Morawski et al., 2000; Cherry et al., 1999; Petrounia and Arnold, 2 ...
Jesse Rabinowitz - National Academy of Sciences
... physiology positioned him to recognize novel questions in the area of translation. For example, the initiation of protein biosynthesis was believed to require formylation of the initiator methionyl-tRNA both in bacteria and eukaryotic organelles. This transformylation reaction of met-tRNAi utilizes ...
... physiology positioned him to recognize novel questions in the area of translation. For example, the initiation of protein biosynthesis was believed to require formylation of the initiator methionyl-tRNA both in bacteria and eukaryotic organelles. This transformylation reaction of met-tRNAi utilizes ...
Molecular analysis and biological implications of STAT3 signal
... whether scaffold proteins are also involved in IL-6-induced activation of the Vav-RacMEKK-SEK/MKK-4-PKCδ signal transduction cascade and determine their role in IL-6induced STAT3 ser727 phosphorylation. In HepG2 cells, IL-6-induced STAT3 signaling is transient. Within 60 min, both STAT3 tyr705 and s ...
... whether scaffold proteins are also involved in IL-6-induced activation of the Vav-RacMEKK-SEK/MKK-4-PKCδ signal transduction cascade and determine their role in IL-6induced STAT3 ser727 phosphorylation. In HepG2 cells, IL-6-induced STAT3 signaling is transient. Within 60 min, both STAT3 tyr705 and s ...
Identification of a Vinyl Reductase Gene for
... genes have been identified in higher plants. However, only the gene for 3,8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), which is indispensable for monovinyl chlorophyll synthesis, has not been identified yet. In this study, we isolated an Arabidopsis thaliana mutant that accumulated divin ...
... genes have been identified in higher plants. However, only the gene for 3,8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), which is indispensable for monovinyl chlorophyll synthesis, has not been identified yet. In this study, we isolated an Arabidopsis thaliana mutant that accumulated divin ...
The Three-Dimensional Structure of Aspergillus niger Pectin Lyase
... can be isolated in two conformational states, one at pH 6.0 and the other at pH 7.5. Unlike the pH 6.0 state, the activity of the pH 7.5 state is dependent upon ionic strength and undergoes a reversible but inactivating change. The pectin lyases belong to a protein superfamily that includes the Pels ...
... can be isolated in two conformational states, one at pH 6.0 and the other at pH 7.5. Unlike the pH 6.0 state, the activity of the pH 7.5 state is dependent upon ionic strength and undergoes a reversible but inactivating change. The pectin lyases belong to a protein superfamily that includes the Pels ...
Organic Chemistry/Fourth Edition: e-Text
... Electrophoresis is used primarily to analyze mixtures of peptides and proteins, rather than individual amino acids, but analogous principles apply. Because they incorporate different numbers of amino acids and because their side chains are different, two peptides will have slightly different acid–ba ...
... Electrophoresis is used primarily to analyze mixtures of peptides and proteins, rather than individual amino acids, but analogous principles apply. Because they incorporate different numbers of amino acids and because their side chains are different, two peptides will have slightly different acid–ba ...
Structure and Antioxidant Catalytic Function of Plant Glutathione Trans
... control cell proliferation and cell death, regulation of the metabolic pathways, bind non-catalytically and transfer a wide range of endogenous and exogenous ligands [5,6,29,30,31]. For example, the isoenzyme GSTP1-1 from human is an ubiquitously expressed protein that plays an important role in the ...
... control cell proliferation and cell death, regulation of the metabolic pathways, bind non-catalytically and transfer a wide range of endogenous and exogenous ligands [5,6,29,30,31]. For example, the isoenzyme GSTP1-1 from human is an ubiquitously expressed protein that plays an important role in the ...
Evolution - Amino Acid wksht
... Proteins are made of amino acids. DNA determines what sequence these amino acids must be in to build the protein. Scientists can examine the amino acid sequence of particular protein molecules found in vertebrates to determine the degree of similarity between species. Even organisms that appear to h ...
... Proteins are made of amino acids. DNA determines what sequence these amino acids must be in to build the protein. Scientists can examine the amino acid sequence of particular protein molecules found in vertebrates to determine the degree of similarity between species. Even organisms that appear to h ...
The demonstration of nickel in the urease of Helicobacter pylori by
... H. pylori urease would be expected because of the inhibition of enzyme activity by acetohydroxamic acid [7]. It is known that the hydroxamate moiety binds to this metal ion [4]. Mooney et al. [81 have shown that acetohydroxamic acid abolished the urea/urease dependent acid resistance of H. pylori at ...
... H. pylori urease would be expected because of the inhibition of enzyme activity by acetohydroxamic acid [7]. It is known that the hydroxamate moiety binds to this metal ion [4]. Mooney et al. [81 have shown that acetohydroxamic acid abolished the urea/urease dependent acid resistance of H. pylori at ...
Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin
... porcine, and human TPO. All the potential N-linked glycosylation sites are located in the C-terminal half of theprotein. To determine whether the two splice forms of TPO are expressed, cells transfected with expression vectors encoding human TPO or TPO-2 wereincubated in the presence of [3sS]-labele ...
... porcine, and human TPO. All the potential N-linked glycosylation sites are located in the C-terminal half of theprotein. To determine whether the two splice forms of TPO are expressed, cells transfected with expression vectors encoding human TPO or TPO-2 wereincubated in the presence of [3sS]-labele ...
Cloning and functional expression of invertebrate connexins from
... encoded by innexins, and the name innexin itself was originally defined as the invertebrate analog of connexin [11]. The availability of genomic and cDNA databases from an invertebrate chordate, Ciona intestinalis [16,17], has questioned this potential misconception. A recent survey of cellular junct ...
... encoded by innexins, and the name innexin itself was originally defined as the invertebrate analog of connexin [11]. The availability of genomic and cDNA databases from an invertebrate chordate, Ciona intestinalis [16,17], has questioned this potential misconception. A recent survey of cellular junct ...
- Wiley Online Library
... detected (upper smear in A, B, C, lanes 2). Furthermore, several culture independent negative staining bands could be detected which were also amino acid independent. Omitting NAD from the reaction mix gave the same results demonstrating that the amino acid independent positive and negative staining ...
... detected (upper smear in A, B, C, lanes 2). Furthermore, several culture independent negative staining bands could be detected which were also amino acid independent. Omitting NAD from the reaction mix gave the same results demonstrating that the amino acid independent positive and negative staining ...
projects - Computer Science and Engineering
... Sometimes both alleles are useful in a population, and it helps to have both around A simple example is when diversity is important (the two variants help maintain diversity) Bipolar disorder genes could be under balancing selection ...
... Sometimes both alleles are useful in a population, and it helps to have both around A simple example is when diversity is important (the two variants help maintain diversity) Bipolar disorder genes could be under balancing selection ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.