Identification of Novel Non-Metal Haloperoxidases from the Marine
... restriction enzymes and cloned into pHPO11172 plasmid vector pretreated with the same restriction enzymes. After transforming E. coli JM109 strain with the library, the transformants were cultivated on LB plates containing ampicillin as antibiotics to construct the HPO cassette PCR metagenomic libra ...
... restriction enzymes and cloned into pHPO11172 plasmid vector pretreated with the same restriction enzymes. After transforming E. coli JM109 strain with the library, the transformants were cultivated on LB plates containing ampicillin as antibiotics to construct the HPO cassette PCR metagenomic libra ...
Practical Aspects of Estimating Energy Components in
... Purified ten targeted proteins from sera of patients with various types of advanced stage(stage III-IV)cancers. These proteins were identified as isoforms of the human serum amyloid protein A (SAA) family with or without truncations at their N-terminals. ...
... Purified ten targeted proteins from sera of patients with various types of advanced stage(stage III-IV)cancers. These proteins were identified as isoforms of the human serum amyloid protein A (SAA) family with or without truncations at their N-terminals. ...
Mechanistic insights into the regulation of metabolic enzymes by
... (NAM) and trichostatin A (TSA)—and substitution to an acetylmimetic glutamine residue (K88Q) were found to decrease the affinity for carbamoyl phosphate and the maximum velocity, ...
... (NAM) and trichostatin A (TSA)—and substitution to an acetylmimetic glutamine residue (K88Q) were found to decrease the affinity for carbamoyl phosphate and the maximum velocity, ...
Two nearly identical terpene synthases catalyze the
... Figure 2. Relatedness of AmNES/LIS proteins to other terpene synthases. (a) Alignment of AmNES/LIS-1 (GenBank accession no. EF433761) and AmNES/LIS-2 (GenBank accession no. EF433762) predicted amino acid sequences with snapdragon myrcene synthase (AAO41726), (E)-b-ocimene synthase (AAO42614), strawb ...
... Figure 2. Relatedness of AmNES/LIS proteins to other terpene synthases. (a) Alignment of AmNES/LIS-1 (GenBank accession no. EF433761) and AmNES/LIS-2 (GenBank accession no. EF433762) predicted amino acid sequences with snapdragon myrcene synthase (AAO41726), (E)-b-ocimene synthase (AAO42614), strawb ...
Lipoteichoic Acid Synthesis and Function in Gram
... has significantly diverged from the LtaS enzyme. In Bacillus spp., which contain four LTA synthase enzymes, these proteins are more closely related to each other and the LtaS protein of L. monocytogenes than to LtaP. Disaccharide-containing glycolipids are often the predominant glycolipids and are pr ...
... has significantly diverged from the LtaS enzyme. In Bacillus spp., which contain four LTA synthase enzymes, these proteins are more closely related to each other and the LtaS protein of L. monocytogenes than to LtaP. Disaccharide-containing glycolipids are often the predominant glycolipids and are pr ...
C H A P
... because the fatty acids produced by GehA activity on sebaceous triglycerides induce severe inflammation, and are involved in the plugging of the sebaceous follicle ducts and in biofilm formation (Higaki, 2003; General Introduction 3.4.1). For this reason, several efforts have been performed for the ...
... because the fatty acids produced by GehA activity on sebaceous triglycerides induce severe inflammation, and are involved in the plugging of the sebaceous follicle ducts and in biofilm formation (Higaki, 2003; General Introduction 3.4.1). For this reason, several efforts have been performed for the ...
Design and Evaluation of Hydrophobic Ion
... Preparation of lipid-polymer hybrid nanoparticle (LPNs) The LPNs were prepared by a solid-in-oil-in-water (s/o/w) emulsification-solvent-evaporation method. Briefly, 200 mg PCL and 45 mg PC:glyceryl tripalmitate mixture were dissolved in 8 ml DCM:acetone mixture as organic solvents to form the oil p ...
... Preparation of lipid-polymer hybrid nanoparticle (LPNs) The LPNs were prepared by a solid-in-oil-in-water (s/o/w) emulsification-solvent-evaporation method. Briefly, 200 mg PCL and 45 mg PC:glyceryl tripalmitate mixture were dissolved in 8 ml DCM:acetone mixture as organic solvents to form the oil p ...
Prokaryotic features of a nucleus
... the enzymes from lobster [29 - 311 and B. stearothermophilus [16] demonstrated that the aminoterminal and carboxyterminal moieties of the GAPDH polypeptide are organized as two independent three-dimensional units, which were highly conserved during the divergence of prokaryotes and eukaryotes: the c ...
... the enzymes from lobster [29 - 311 and B. stearothermophilus [16] demonstrated that the aminoterminal and carboxyterminal moieties of the GAPDH polypeptide are organized as two independent three-dimensional units, which were highly conserved during the divergence of prokaryotes and eukaryotes: the c ...
ribosomal defects in a mutant deficient in the yajl homolog of the
... chaperones that, upon oxidative stress, form mixed disulfides with proteins containing essential cysteines for catalysis, such as glyceraldehyde-3-phosphate dehydrogenase and peroxidases, or FeS cluster binding, such as aconitases and the NuoG subunit of NADH dehydrogenase 1 (Le et al., 2012). Moreo ...
... chaperones that, upon oxidative stress, form mixed disulfides with proteins containing essential cysteines for catalysis, such as glyceraldehyde-3-phosphate dehydrogenase and peroxidases, or FeS cluster binding, such as aconitases and the NuoG subunit of NADH dehydrogenase 1 (Le et al., 2012). Moreo ...
Biological ontologies for human functional annotation and
... added value compared to GENIA / thesaurus approach Empirical Studies to create evidence of whether • Formal Ontologies better serve the needs of knowledge annotation / processing in biomedicine • Informal Thesauri are sufficient ...
... added value compared to GENIA / thesaurus approach Empirical Studies to create evidence of whether • Formal Ontologies better serve the needs of knowledge annotation / processing in biomedicine • Informal Thesauri are sufficient ...
Studies on the key amino acid residues responsible for the alkali
... residue with hydroxyl side-chain caused a shift in the pH optimum of the xylanase and a decrease in its activity by ∼20%. All of these suggested that Asn-71 might be involved in the catalytic active center and was critical for the alkaline tolerance of the xylanase. In order to search other residues ...
... residue with hydroxyl side-chain caused a shift in the pH optimum of the xylanase and a decrease in its activity by ∼20%. All of these suggested that Asn-71 might be involved in the catalytic active center and was critical for the alkaline tolerance of the xylanase. In order to search other residues ...
Understanding the Regulation of Metabolic Enzyme Acetylation in E
... Global protein acetylation is a newly discovered phenomenon in bacteria. Of the more than 250 acetylations reported in E. coli, many are of metabolic enzymes [1-3]. Thus, acetylation could represent a novel posttranslational mechanism of metabolic control. Yet, almost nothing is known about the regu ...
... Global protein acetylation is a newly discovered phenomenon in bacteria. Of the more than 250 acetylations reported in E. coli, many are of metabolic enzymes [1-3]. Thus, acetylation could represent a novel posttranslational mechanism of metabolic control. Yet, almost nothing is known about the regu ...
Eater, a Transmembrane Protein Mediating
... not shown), and could be competed with a 10-fold excess of unlabeled Eater 1–199 His protein (Figures 3B and 3D), while excess GST-His control protein had no effect (Figure 3D). Moreover, typical scavenger receptor ligands such as oxidized and acetylated low-density lipoprotein (LDL; see Greaves and ...
... not shown), and could be competed with a 10-fold excess of unlabeled Eater 1–199 His protein (Figures 3B and 3D), while excess GST-His control protein had no effect (Figure 3D). Moreover, typical scavenger receptor ligands such as oxidized and acetylated low-density lipoprotein (LDL; see Greaves and ...
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... the formation of carcinogenic and toxic disinfection byproducts, which impose a health, safety, and environment (HSE) concern. Second, it is known that SRB can produce extracellular polymeric substances (EPS), which, besides assisting in calcium precipitation and mineralization [31], also serve to e ...
... the formation of carcinogenic and toxic disinfection byproducts, which impose a health, safety, and environment (HSE) concern. Second, it is known that SRB can produce extracellular polymeric substances (EPS), which, besides assisting in calcium precipitation and mineralization [31], also serve to e ...
Pax8, a murine paired box gene expressed in the developing
... probes (Deutsch, unpublished). DNA sequencing revealed that this clone had part of a novel paired box. A 112 bp fragment containing the partial paired box sequence of c%0 was labeled by random priming (Feinberg and Vogelstein, 1983) and used to screen 6xlCr clones of the same cDNA library under high ...
... probes (Deutsch, unpublished). DNA sequencing revealed that this clone had part of a novel paired box. A 112 bp fragment containing the partial paired box sequence of c%0 was labeled by random priming (Feinberg and Vogelstein, 1983) and used to screen 6xlCr clones of the same cDNA library under high ...
The introduction and use of PKU sphere™, a Glycomacropeptide
... PS have been Phe-free L-amino acid supplements (L-AA supplements). They were originally developed using human breast milk as the reference protein. Dietary treatment of PKU is multifaceted, challenging and lifelong2-4. Key dietary behaviours associated with optimal control of blood Phe levels includ ...
... PS have been Phe-free L-amino acid supplements (L-AA supplements). They were originally developed using human breast milk as the reference protein. Dietary treatment of PKU is multifaceted, challenging and lifelong2-4. Key dietary behaviours associated with optimal control of blood Phe levels includ ...
Journal of Biological Chemistry
... promoter as well as to facilitate its purification, the zntA gene was subcloned with a carboxyl-terminal histidyl tag into the expression vector pBAD/Myc-His C, where it is under control of the tightly regulated ara promoter. ZntA was expressed in the E. coli strains Top10 and LMG194 as well as the ...
... promoter as well as to facilitate its purification, the zntA gene was subcloned with a carboxyl-terminal histidyl tag into the expression vector pBAD/Myc-His C, where it is under control of the tightly regulated ara promoter. ZntA was expressed in the E. coli strains Top10 and LMG194 as well as the ...
H2S-NSAIDs
... the H2S catabolic pathway is not well-defined yet, and most of the data have been obtained by using exogenous H2S. CBS and CSE are responsible for the majority of the endogenous production of H2S in mammalian tissues and have been detected in human and other mammalian cells.(27) Their expression is ...
... the H2S catabolic pathway is not well-defined yet, and most of the data have been obtained by using exogenous H2S. CBS and CSE are responsible for the majority of the endogenous production of H2S in mammalian tissues and have been detected in human and other mammalian cells.(27) Their expression is ...
Early bioenergetic evolution
... was liquid water on the Earth [22], and it continues today because, relative to the amount of water on the Earth, the amount of Fe2þ in the crust is inexhaustible. The Mg(OH)2 in equation (2.1) produces alkaline hydrothermal fluid in agreement with the pH observed for Lost City effluent [17]. Lost C ...
... was liquid water on the Earth [22], and it continues today because, relative to the amount of water on the Earth, the amount of Fe2þ in the crust is inexhaustible. The Mg(OH)2 in equation (2.1) produces alkaline hydrothermal fluid in agreement with the pH observed for Lost City effluent [17]. Lost C ...
glucuronidation of opioids, carboxylic acid
... UGT isoforms. UGTs that are members of the UGT1 gene complex share common second through fifth exons, with at least 12 separate first exons coding for proteins with unique amino-terminal domains (2). In contrast, gene products of the UGT2 family appear to be transcribed from unique genes (3, 4). Alt ...
... UGT isoforms. UGTs that are members of the UGT1 gene complex share common second through fifth exons, with at least 12 separate first exons coding for proteins with unique amino-terminal domains (2). In contrast, gene products of the UGT2 family appear to be transcribed from unique genes (3, 4). Alt ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.