Purification and Characterization of
... buffer containing 1 mm DTT. Fractions showing enzyme activity were pooled and concentrated to one-tenth the volume by high-pressure ultrafiltration using an Amicon (Beverly, MA) model 12 stirred cell equipped with a PM30 Diaflo membrane at a maximum pressure (60 p.s.i.) of N2. The sample was activat ...
... buffer containing 1 mm DTT. Fractions showing enzyme activity were pooled and concentrated to one-tenth the volume by high-pressure ultrafiltration using an Amicon (Beverly, MA) model 12 stirred cell equipped with a PM30 Diaflo membrane at a maximum pressure (60 p.s.i.) of N2. The sample was activat ...
Epsilon glutathione transferases possess a unique class
... In the present study, we have determined the structure of DmGSTE6 to supplement the currently known Epsilon structures (Figure 1, Table 1). This structure adopts the typically highly conserved GST fold with two domains comprising a βαβαββα motif in the N-terminus and an all α-helical domain in the C ...
... In the present study, we have determined the structure of DmGSTE6 to supplement the currently known Epsilon structures (Figure 1, Table 1). This structure adopts the typically highly conserved GST fold with two domains comprising a βαβαββα motif in the N-terminus and an all α-helical domain in the C ...
Saccharomyces cerevisiae.
... However, there are still organisms that can grow anaerobically, and even those that can survive under both conditions. The question is what the difference is between these organisms. Why can some grow only in the presence of molecular oxygen, some only in the absence, and are some able to withstand ...
... However, there are still organisms that can grow anaerobically, and even those that can survive under both conditions. The question is what the difference is between these organisms. Why can some grow only in the presence of molecular oxygen, some only in the absence, and are some able to withstand ...
NIH Public Access
... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
Electrophoresis Basi..
... Isozymes (isoenzymes): enzymes that differ in amino acid sequence but catalyze the same chemical reaction. Isozymes have different molecular weights and chemical properties and thus can be separated from each other during electrophoresis. Isozymes are coded by genes on different ...
... Isozymes (isoenzymes): enzymes that differ in amino acid sequence but catalyze the same chemical reaction. Isozymes have different molecular weights and chemical properties and thus can be separated from each other during electrophoresis. Isozymes are coded by genes on different ...
Unit 4– Cerebrospinal fluid (CSF)
... – only a small volume is available – do not require pre-processing ...
... – only a small volume is available – do not require pre-processing ...
Some Structural and Kinetic Aspects of L
... binding of PEP to A domain of PK subunit. Also M2, R and L isoenzymes exhibit a sigmoidal kinetics toward the substrate PEP. This means that this substrate is at the same time homotropic cooperativity effector (Munoz & Ponce, 2003; Gunasekaran et al., 2004; Koshland & Hamadani, 2002; Ainslie et al., ...
... binding of PEP to A domain of PK subunit. Also M2, R and L isoenzymes exhibit a sigmoidal kinetics toward the substrate PEP. This means that this substrate is at the same time homotropic cooperativity effector (Munoz & Ponce, 2003; Gunasekaran et al., 2004; Koshland & Hamadani, 2002; Ainslie et al., ...
Stockholm University
... Galanin is a neuropeptide found throughout the central and peripheral nervous systems of a wide range of species, ranging from human and mouse to frog and tuna. Galanin mediates its physiological roles through three receptors (GalR1–3), all members of the G-protein coupled receptor family. In mappin ...
... Galanin is a neuropeptide found throughout the central and peripheral nervous systems of a wide range of species, ranging from human and mouse to frog and tuna. Galanin mediates its physiological roles through three receptors (GalR1–3), all members of the G-protein coupled receptor family. In mappin ...
Phosphatidylglycerol-containing ER
... contacts between organelles could accommodate transfer of calcium, the process of mitochondrial membranes repair and restitution, and the demand to coordinate nuclear gene expression and maintain regulatory processes in response to cellular need for ATP synthesis were not plausible [3,4,16-18]. The ...
... contacts between organelles could accommodate transfer of calcium, the process of mitochondrial membranes repair and restitution, and the demand to coordinate nuclear gene expression and maintain regulatory processes in response to cellular need for ATP synthesis were not plausible [3,4,16-18]. The ...
Biomolecules
... • Biomolecules are molecules that are present in living organisms, including large macromolecules such as proteins, lipids, carbohydrates, and nucleic acids. ...
... • Biomolecules are molecules that are present in living organisms, including large macromolecules such as proteins, lipids, carbohydrates, and nucleic acids. ...
ester formation in brewery fermentations
... produced, which can result in beers with little flavour. The factors influencing ester production are reviewed, together with the ways in which they can be used to control ester synthesis. It is believed that acetate esters are synthesized by an enzyme called alcohol acetyl transferase (AAT) which u ...
... produced, which can result in beers with little flavour. The factors influencing ester production are reviewed, together with the ways in which they can be used to control ester synthesis. It is believed that acetate esters are synthesized by an enzyme called alcohol acetyl transferase (AAT) which u ...
OMPROT PP2 ver4 - Plant Physiology
... outer membranes by sedimentation and subsequent flotation through sucrose gradients. This membrane fractionation was performed on two samples, the first being FFE enriched mitochondria to yield enriched mitochondrial outer membrane (Mt OM), the second being the crude organelle enrichment HSP to yie ...
... outer membranes by sedimentation and subsequent flotation through sucrose gradients. This membrane fractionation was performed on two samples, the first being FFE enriched mitochondria to yield enriched mitochondrial outer membrane (Mt OM), the second being the crude organelle enrichment HSP to yie ...
Novel Expression Pattern of Cytosolic Gln
... material is degraded by the plant, according to terminology established by Ribeiro et al. (1995). Frankiainfected tissue was distinguishable in polarizing optics from surrounding uninfected cortical tissue by the absence of birefringence associated with secondary cell walls or starch grains (Fig. 3B ...
... material is degraded by the plant, according to terminology established by Ribeiro et al. (1995). Frankiainfected tissue was distinguishable in polarizing optics from surrounding uninfected cortical tissue by the absence of birefringence associated with secondary cell walls or starch grains (Fig. 3B ...
Skeletal muscle phenotype affects fasting
... rates of oxidative phosphorylation and mitochondrial efficiency were measured in pectoralis (glycolytic) and gastrocnemius (oxidative) muscles from cold-acclimated ducklings (Cairina moschata). Pyruvate and palmitoyl-L-carnitine were used in the presence of malate as respiratory substrates. Plasma m ...
... rates of oxidative phosphorylation and mitochondrial efficiency were measured in pectoralis (glycolytic) and gastrocnemius (oxidative) muscles from cold-acclimated ducklings (Cairina moschata). Pyruvate and palmitoyl-L-carnitine were used in the presence of malate as respiratory substrates. Plasma m ...
Cholesterol Synthesis
... Long-term regulation is by varied formation and degradation of HMG-CoA Reductase and other enzymes of the pathway for synthesis of cholesterol. Regulated proteolysis of HMG-CoA Reductase: • Degradation of HMG-CoA Reductase is stimulated by cholesterol, oxidized derivatives of cholesterol, mevalon ...
... Long-term regulation is by varied formation and degradation of HMG-CoA Reductase and other enzymes of the pathway for synthesis of cholesterol. Regulated proteolysis of HMG-CoA Reductase: • Degradation of HMG-CoA Reductase is stimulated by cholesterol, oxidized derivatives of cholesterol, mevalon ...
Dietary Supplements and Military Divers
... and physicians are not trained in this area. As such, many may feel uncomfortable with their lack of familiarity regarding the ever-increasing supplement pharmacopoeia. Peer-reviewed scientific research and evidenced-based information are often limited. Moreover, because the general public and activ ...
... and physicians are not trained in this area. As such, many may feel uncomfortable with their lack of familiarity regarding the ever-increasing supplement pharmacopoeia. Peer-reviewed scientific research and evidenced-based information are often limited. Moreover, because the general public and activ ...
Calcitonin Gene-Related Peptides
... vasodilatory action and its ionotropic and chronotropic effects, CGRP is likely to play a role in cardiovascular homeostasis. Furthermore, it influences feeding and digestion since it has shown to decrease food intake, gastric secretion, and intestinal motility. Based on its ability to modulate subs ...
... vasodilatory action and its ionotropic and chronotropic effects, CGRP is likely to play a role in cardiovascular homeostasis. Furthermore, it influences feeding and digestion since it has shown to decrease food intake, gastric secretion, and intestinal motility. Based on its ability to modulate subs ...
Cloning of adiponectin receptors that mediate antidiabetic metabolic
... cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocytes (Supplementary ...
... cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocytes (Supplementary ...
Docking of B-cell epitope antigen to specific hepatitis B antibody
... antigen and in understanding where important regions of an antigen are located in its three-dimensional structure. Therefore, mapping the interaction surface between antibody and antigen that bind each other is of outstanding interest in molecular immunology. Complementary to various experimental te ...
... antigen and in understanding where important regions of an antigen are located in its three-dimensional structure. Therefore, mapping the interaction surface between antibody and antigen that bind each other is of outstanding interest in molecular immunology. Complementary to various experimental te ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... the earliest eukaryotic cells (Martin and Müller 1998). In principle, three alternative explanations could currently account for the distribution of PFO among eukaryotes and its evident correlation with the amitochondriate phenotype. The first possible explanation is that the host of mitochondrial ...
... the earliest eukaryotic cells (Martin and Müller 1998). In principle, three alternative explanations could currently account for the distribution of PFO among eukaryotes and its evident correlation with the amitochondriate phenotype. The first possible explanation is that the host of mitochondrial ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.