Moore BA, Gonzalez Aviles GD, Larkins CE, Hillman MJ, Caspary T. Mamm Genome. 2010 Aug;21(7-8):350-60. Mitochondrial retention of Opa1 is required for mouse embryogenesis
... Fig. 3 Mislocalization of Opa1 protein in lilR3 embryos. Western blot analysis of mitochondrial (left panels) and cytosolic (right panels) E10.5 embryo protein lysates using antibodies against Opa1, a-tubulin (loading control), and cytochrome c (mitochondrial marker, demonstrating the efficiency of ...
... Fig. 3 Mislocalization of Opa1 protein in lilR3 embryos. Western blot analysis of mitochondrial (left panels) and cytosolic (right panels) E10.5 embryo protein lysates using antibodies against Opa1, a-tubulin (loading control), and cytochrome c (mitochondrial marker, demonstrating the efficiency of ...
Silica Particles
... Low autofluorescence and low nonspecific binding of many biomolecules Suitable for applications over 1000˚C Wide range of solvent compatibility SPHEROTM Silica Nano Superparamagnetic Particles Encapsulated silica layer eliminates exposed iron oxide on the surface Ensure rapid magnetic mobility and e ...
... Low autofluorescence and low nonspecific binding of many biomolecules Suitable for applications over 1000˚C Wide range of solvent compatibility SPHEROTM Silica Nano Superparamagnetic Particles Encapsulated silica layer eliminates exposed iron oxide on the surface Ensure rapid magnetic mobility and e ...
Nonribosomal peptide synthesis in Aspergillus
... NRPS and polyketide biosynthesis (Mootz et al., 2002). 49-PPTase sequences exhibit limited regional homology to each other, and one such homology motif is WxxKEAxxK (Lambalot et al., 1996). Many 49-PPTases have been identified in bacteria; however, until recently few have been found in filamentous f ...
... NRPS and polyketide biosynthesis (Mootz et al., 2002). 49-PPTase sequences exhibit limited regional homology to each other, and one such homology motif is WxxKEAxxK (Lambalot et al., 1996). Many 49-PPTases have been identified in bacteria; however, until recently few have been found in filamentous f ...
Studying the Interaction Profiles of Nonnatural Amino Acids –
... In contrast, diverse α-amino acid patterns were found with recognition specificity for βγfoldameric sequences, at the interface of parallel or antiparallel helical assemblies. Here, the sequences selected by phage display show that the incorporation of a polar H-bond donor functionality can signific ...
... In contrast, diverse α-amino acid patterns were found with recognition specificity for βγfoldameric sequences, at the interface of parallel or antiparallel helical assemblies. Here, the sequences selected by phage display show that the incorporation of a polar H-bond donor functionality can signific ...
Life in the slow lane: molecular mechanisms of estivation
... Estivation is a state of aerobic hypometabolism used by organisms to endure seasonally arid conditions, often in desert environments. Estivating species are often active for only a few weeks each year to feed and breed and then retreat to estivate in sheltered sites, often underground. In general, e ...
... Estivation is a state of aerobic hypometabolism used by organisms to endure seasonally arid conditions, often in desert environments. Estivating species are often active for only a few weeks each year to feed and breed and then retreat to estivate in sheltered sites, often underground. In general, e ...
Dynamic Model of the Process of Protein Synthesis in Eukaryotic Cells
... Protein synthesis is the final step of gene expression in all cells. In order to understand the regulation of this process, it is important to have an accurate model that incorporates the regulatory steps. The model presented in this paper is composed of set of differential equations which describe ...
... Protein synthesis is the final step of gene expression in all cells. In order to understand the regulation of this process, it is important to have an accurate model that incorporates the regulatory steps. The model presented in this paper is composed of set of differential equations which describe ...
E. Coli MurG: A Paradigm for a Superfamily of
... residues. The alignment shows that a relatively small number of residues are invariant across the range of MurG homologs (Fig. (5)). These residues are confined to five different regions, three of which are the glycine-rich loops that are proposed to be involved in binding the diphosphates of the su ...
... residues. The alignment shows that a relatively small number of residues are invariant across the range of MurG homologs (Fig. (5)). These residues are confined to five different regions, three of which are the glycine-rich loops that are proposed to be involved in binding the diphosphates of the su ...
CASE 37
... above occur, but there are several longer term responses to insulin. For example, glucokinase is a liver-specific enzyme that is required for glucose phosphorylation to glucose-6-phosphate. The synthesis of glucokinase is insulin dependent. The actual turnover of the protein is such that during a no ...
... above occur, but there are several longer term responses to insulin. For example, glucokinase is a liver-specific enzyme that is required for glucose phosphorylation to glucose-6-phosphate. The synthesis of glucokinase is insulin dependent. The actual turnover of the protein is such that during a no ...
NOVEL SPERM-SPECIFIC GLYCOLYTIC ISOZYMES GENERATED
... Targeted gene disruption of glycolytic enzymes expressed only during spermatogenesis indicates that glycolysis is essential for sperm motility and male fertility. This pathway is compartmentalized in the principal piece of the sperm flagellum, where several spermatogenic cell-specific isozymes are b ...
... Targeted gene disruption of glycolytic enzymes expressed only during spermatogenesis indicates that glycolysis is essential for sperm motility and male fertility. This pathway is compartmentalized in the principal piece of the sperm flagellum, where several spermatogenic cell-specific isozymes are b ...
THE SARCOTUBULAR SYSTEM OF FROG
... is called the sarcotubular system. By means of differential centrifugation it has been possible to isolate from the frog muscle homogenate a fraction composed of small vesicles, tubules, and particles. This fraction is without cytochrome oxidase activity, which is localized in the mitochondrial memb ...
... is called the sarcotubular system. By means of differential centrifugation it has been possible to isolate from the frog muscle homogenate a fraction composed of small vesicles, tubules, and particles. This fraction is without cytochrome oxidase activity, which is localized in the mitochondrial memb ...
Malate Dehydrogenase
... and a similar tertiary structure. Two isoenzymes of MDH, mitochondrial and cytoplasmic, are present in mammalian cells. In higher plants, there are three NADdependent forms located in the cytoplasm, the mitochondria and the microbodies, and one NADP-dependent form present in chloroplasts (Poeydomeng ...
... and a similar tertiary structure. Two isoenzymes of MDH, mitochondrial and cytoplasmic, are present in mammalian cells. In higher plants, there are three NADdependent forms located in the cytoplasm, the mitochondria and the microbodies, and one NADP-dependent form present in chloroplasts (Poeydomeng ...
Degradable heterobifunctional poly (ethylene glycol) acrylates and
... dri and Bolikal in J. Org. Chem, 60, 331-336 (1995) recite that the Water-insoluble drug taxol, When coupled to PEG, becomes Water soluble. Davis et al. in US. Pat. No. 4,179, 337 recite that proteins coupled to PEG have an enhanced blood circulation lifetime because of a reduced rate of ...
... dri and Bolikal in J. Org. Chem, 60, 331-336 (1995) recite that the Water-insoluble drug taxol, When coupled to PEG, becomes Water soluble. Davis et al. in US. Pat. No. 4,179, 337 recite that proteins coupled to PEG have an enhanced blood circulation lifetime because of a reduced rate of ...
chapter 1: exploring life
... 4. Draw and label a simplified model of an atom. Explain how this model simplifies our understanding of atomic structure. 5. Distinguish between each of the following pairs of terms: neutron and proton atomic number and mass number atomic weight and mass number 6. Explain how the atomic number and m ...
... 4. Draw and label a simplified model of an atom. Explain how this model simplifies our understanding of atomic structure. 5. Distinguish between each of the following pairs of terms: neutron and proton atomic number and mass number atomic weight and mass number 6. Explain how the atomic number and m ...
Analysis of the Role of Mitochondria of Sake in Fermentation Technologies
... As described above, genes involved in oxidative respiration are repressed in the presence of fermentable sugars and in the absence of oxygen. Similar to this regulation, several genes are known to be induced in response to hypoxia. In contrast to upregulation of the mitochondrial genes in response t ...
... As described above, genes involved in oxidative respiration are repressed in the presence of fermentable sugars and in the absence of oxygen. Similar to this regulation, several genes are known to be induced in response to hypoxia. In contrast to upregulation of the mitochondrial genes in response t ...
AMINO ACID SEQUENCE ANALYSIS OF HUMAN INTERLEUKIN 1
... line, P388D1, has a low isoelectric point (pI) and exhibits micro-charge heterogeneity in the range of 4.9-5.2 (11). P388Dl-derived IL-1 has been purified (1 1), and a complementary DNA (cDNA) 1 coding for the precursor form of this molecule has been recently cloned (12). The nucleic acid sequence o ...
... line, P388D1, has a low isoelectric point (pI) and exhibits micro-charge heterogeneity in the range of 4.9-5.2 (11). P388Dl-derived IL-1 has been purified (1 1), and a complementary DNA (cDNA) 1 coding for the precursor form of this molecule has been recently cloned (12). The nucleic acid sequence o ...
The enzymes of biotin dependent CO2 metabolism: What structures
... /,W angles of -119 and 122 , and this helix-turn-helix motif is a hallmark of proteins belonging to the ATP grasp superfamily as discussed below. Shown in Figure 2(b) is a ribbon representation of the biotin carboxylase dimer. The B-domains for each subunit are located on opposite edges of the dim ...
... /,W angles of -119 and 122 , and this helix-turn-helix motif is a hallmark of proteins belonging to the ATP grasp superfamily as discussed below. Shown in Figure 2(b) is a ribbon representation of the biotin carboxylase dimer. The B-domains for each subunit are located on opposite edges of the dim ...
The Major Component of the Paraflagellar Rod of Trypanosoma
... et al., 1985) using lysogenic strains on E. coil Y 1089. The cells were harvested and suspended in 100 mM Tris-HCl, pH 7.4, 2 mM EDTA and frozen down quickly in ethanol-CO2. A crude lysate was obtained by slow thawing of the cells. As a purification step, an ammonium sulfate precipitation with a fin ...
... et al., 1985) using lysogenic strains on E. coil Y 1089. The cells were harvested and suspended in 100 mM Tris-HCl, pH 7.4, 2 mM EDTA and frozen down quickly in ethanol-CO2. A crude lysate was obtained by slow thawing of the cells. As a purification step, an ammonium sulfate precipitation with a fin ...
mTOR complex 2 (mTORC2) controls hydrophobic motif
... precipitates were then washed twice with Tris lysis buffer containing 0.5 M NaCl, followed by two washes with buffer A. The immunoprecipitates were resuspended in 20 μl of sample buffer, filtered through a 0.22 μm Spin-X filter, and samples were subjected to electrophoresis and immunoblot analysis a ...
... precipitates were then washed twice with Tris lysis buffer containing 0.5 M NaCl, followed by two washes with buffer A. The immunoprecipitates were resuspended in 20 μl of sample buffer, filtered through a 0.22 μm Spin-X filter, and samples were subjected to electrophoresis and immunoblot analysis a ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...
... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...
get ripped or go home!
... Just Pure Products, for Pure Results! NutraBio believes in truth in labeling so we disclose the exact dosage of every ingredient on the label panel. We never hide what's in our products behind bogus proprietary blends or secret formulas and we adhere to all FDA regulations regarding product ...
... Just Pure Products, for Pure Results! NutraBio believes in truth in labeling so we disclose the exact dosage of every ingredient on the label panel. We never hide what's in our products behind bogus proprietary blends or secret formulas and we adhere to all FDA regulations regarding product ...
Quality control in tRNA charging — editing of homocysteine
... All living organisms conduct protein synthesis with a high degree of accuracy maintained in the transmission and flow of information from a gene to protein product. One crucial “quality control” point in maintaining a high level of accuracy is the selectivity by which aminoacyltRNA synthetases furni ...
... All living organisms conduct protein synthesis with a high degree of accuracy maintained in the transmission and flow of information from a gene to protein product. One crucial “quality control” point in maintaining a high level of accuracy is the selectivity by which aminoacyltRNA synthetases furni ...
Chaperone-dependent gene expression of organic
... 3.7. Phylogenetic analysis of S5 lipase The G + C content of a gene encoding a protein may reveal the origin of the protein. The G + C content was calculated using FramePlot 2.3.2 software available at http://www.watson.nih.go.jp. The G + C content of this organic solvent-tolerant lipase gene was 65 ...
... 3.7. Phylogenetic analysis of S5 lipase The G + C content of a gene encoding a protein may reveal the origin of the protein. The G + C content was calculated using FramePlot 2.3.2 software available at http://www.watson.nih.go.jp. The G + C content of this organic solvent-tolerant lipase gene was 65 ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.