Exploration of binding site pattern in arachidonic
... Background: Cyclooxygenase (COXs) and Lipoxygenase (LOXs) pathways are the two major enzymatic pathways in arachidonic acid (AA) metabolism. The term eicosanoid is used to describe biologically active lipid mediators including prostaglandins, thromboxanes, leukotrienes and other oxygenated derivativ ...
... Background: Cyclooxygenase (COXs) and Lipoxygenase (LOXs) pathways are the two major enzymatic pathways in arachidonic acid (AA) metabolism. The term eicosanoid is used to describe biologically active lipid mediators including prostaglandins, thromboxanes, leukotrienes and other oxygenated derivativ ...
Diagnosis and Rectification of Arrested Fermentations
... transporters are proton sympoters, meaning that a proton is taken up for each amino acid molecule transported. The protons must then be extruded from the cell in order to avoid acidification of the cytoplasm. Ethanol leads to increased passive proton flux into the cells. At high ethanol concentratio ...
... transporters are proton sympoters, meaning that a proton is taken up for each amino acid molecule transported. The protons must then be extruded from the cell in order to avoid acidification of the cytoplasm. Ethanol leads to increased passive proton flux into the cells. At high ethanol concentratio ...
Quantum binding energy features of the T3-785
... could be a very useful approach to predict their energetic properties, like a relative free energy of a collagen-model peptide with respect to its monomer state.23 Recently, Rodrigues et al. developed a computer simulation to describe, at the quantum level, the non-covalent interaction energies amon ...
... could be a very useful approach to predict their energetic properties, like a relative free energy of a collagen-model peptide with respect to its monomer state.23 Recently, Rodrigues et al. developed a computer simulation to describe, at the quantum level, the non-covalent interaction energies amon ...
Codon optimization of Col H gene encoding Clostridium
... Recently, making use of Clostridium collagenases has attracted a great deal of researchers’ ...
... Recently, making use of Clostridium collagenases has attracted a great deal of researchers’ ...
rhizopus oryzae - Journal of Marine Science and Technology
... ture [21]. Similar to classification of cellulolytic enzymes, chitinolytic enzymes can be crudely classified into β-Nacetylhexosaminidase (EC 3.2.1.52, known as exochitinases) and 1,4-β-poly-N- acetylglucosaminidase (EC 3.2.1.14, known as endochitinases) [19]. Exochitinases hydrolyze chitin at β-1,4 ...
... ture [21]. Similar to classification of cellulolytic enzymes, chitinolytic enzymes can be crudely classified into β-Nacetylhexosaminidase (EC 3.2.1.52, known as exochitinases) and 1,4-β-poly-N- acetylglucosaminidase (EC 3.2.1.14, known as endochitinases) [19]. Exochitinases hydrolyze chitin at β-1,4 ...
tubulin - Journal of Cell Science
... Positions of insertions and deletions A dramatic sequence deviation concerning a highly conserved tubulin motif occurs for all four known δ-tubulins in loop T7, further emphasizing divergence of this tubulin with respect to the rest. The consensus sequence GxxNxD is changed to YxxN-P. We expect this ...
... Positions of insertions and deletions A dramatic sequence deviation concerning a highly conserved tubulin motif occurs for all four known δ-tubulins in loop T7, further emphasizing divergence of this tubulin with respect to the rest. The consensus sequence GxxNxD is changed to YxxN-P. We expect this ...
Growth, digestive and absorptive capacity and
... capacity may be different among fish with different feeding habits. The present study focused on the effects of threonine on digestive and absorptive capacity of herbivorous grass carp (Ctenopharyngodonidella). The function of fish digestive organ is correlated with its development [9]. Threonine ha ...
... capacity may be different among fish with different feeding habits. The present study focused on the effects of threonine on digestive and absorptive capacity of herbivorous grass carp (Ctenopharyngodonidella). The function of fish digestive organ is correlated with its development [9]. Threonine ha ...
- Wiley Online Library
... of a large family of zinc-containing alcohol dehydrogenases. The primary structures of 47 members of this family have been determined and aligned, and an evolutionary tree has been constructed, assuming a divergent evolution from a common ancestral gene [1]. In this way, it was possible to identify ...
... of a large family of zinc-containing alcohol dehydrogenases. The primary structures of 47 members of this family have been determined and aligned, and an evolutionary tree has been constructed, assuming a divergent evolution from a common ancestral gene [1]. In this way, it was possible to identify ...
Defining the Schistosoma haematobium kinome enables the
... Recent research efforts to identify new molecular targets for chemotherapeutic intervention have focused on protein kinases13,14, because they are involved in signalling cascades of essential regulatory and developmental processes15–17, particular kinase groups have relatively conserved structures18 ...
... Recent research efforts to identify new molecular targets for chemotherapeutic intervention have focused on protein kinases13,14, because they are involved in signalling cascades of essential regulatory and developmental processes15–17, particular kinase groups have relatively conserved structures18 ...
NST110: Advanced Toxicology Lecture 4: Phase I Metabolism
... from a lipophilic form (that favors absorption) to a hydrophilic form (favoring excretion in the urine or bile). The main evolutionary goal of biotransformation is to increase the rate of excretion of xenobiotics or drugs. Biotransformation can detoxify or bioactivate xenobiotics to more toxic forms ...
... from a lipophilic form (that favors absorption) to a hydrophilic form (favoring excretion in the urine or bile). The main evolutionary goal of biotransformation is to increase the rate of excretion of xenobiotics or drugs. Biotransformation can detoxify or bioactivate xenobiotics to more toxic forms ...
Glycolytic strategy as a tradeoff between energy yield and protein cost
... phosphorylated twice and cleaved into two triose-phosphates (G3P and dihydroxyacetone phosphate), both of which are used to produce ATP through substrate-level phosphorylation in lower glycolysis (2, 7) (Fig. 1B). In the ED pathway, glucose is phosphorylated only once and oxidized to 2-keto-3-deoxy- ...
... phosphorylated twice and cleaved into two triose-phosphates (G3P and dihydroxyacetone phosphate), both of which are used to produce ATP through substrate-level phosphorylation in lower glycolysis (2, 7) (Fig. 1B). In the ED pathway, glucose is phosphorylated only once and oxidized to 2-keto-3-deoxy- ...
regulation of mammalian acetyl
... by cAMP-dependent protein kinase and ACC kinase (67) causes primarily an increase in the Ka for citrate and a more modest decrease in Vmax (67). The isolation and characterization of the various phosphopeptides of ACC-α that can be phosphorylated by different protein kinases in vitro and in vivo (32 ...
... by cAMP-dependent protein kinase and ACC kinase (67) causes primarily an increase in the Ka for citrate and a more modest decrease in Vmax (67). The isolation and characterization of the various phosphopeptides of ACC-α that can be phosphorylated by different protein kinases in vitro and in vivo (32 ...
Visualizza/apri
... were separated and then quantified in relative terms. In particular, the activity of μ-calpain and caspase-3 was assessed by the quantification of alpha II spectrin cleavage products of 145 kDa (SBDP145) and of 120 kDa (SBDP120) respectively. Moreover the activity of caspase-3 was assessed also by t ...
... were separated and then quantified in relative terms. In particular, the activity of μ-calpain and caspase-3 was assessed by the quantification of alpha II spectrin cleavage products of 145 kDa (SBDP145) and of 120 kDa (SBDP120) respectively. Moreover the activity of caspase-3 was assessed also by t ...
Microsoft Word
... according to (MacLennan and Asai, 1968) and centrifuged at 48 000 g for 45 min at 4°C. Pellets were suspended at a concentration of 10 mg of starting mitochondrial protein per mL of buffer "A" (150 mM potassium acetate, 10% (V/V) glycerol, 2 mM PMSF, 2 mM e-ACA, 30 mM HEPES pH 7.4 and EDTA-free prot ...
... according to (MacLennan and Asai, 1968) and centrifuged at 48 000 g for 45 min at 4°C. Pellets were suspended at a concentration of 10 mg of starting mitochondrial protein per mL of buffer "A" (150 mM potassium acetate, 10% (V/V) glycerol, 2 mM PMSF, 2 mM e-ACA, 30 mM HEPES pH 7.4 and EDTA-free prot ...
Full-Text PDF
... impact on cells. In E. coli, reduction of tellurite can occur through the actions of nitrate reductases, however, this is a non-specific reaction [8,9]. Other enzymes, such as the thiol:disulfide oxidoreductase of Rhodobacter capsulatus [10], GutS from E. coli [11], among others [12–15] have been im ...
... impact on cells. In E. coli, reduction of tellurite can occur through the actions of nitrate reductases, however, this is a non-specific reaction [8,9]. Other enzymes, such as the thiol:disulfide oxidoreductase of Rhodobacter capsulatus [10], GutS from E. coli [11], among others [12–15] have been im ...
Correlation of an Immobilized Digestive Enzyme Assay with Poultry
... The cost of feed represents >65% of the bird arriving at a US processing plant (July 2005 Agri Stats); therefore a more precise knowledge of the nutritional value of feed ingredients would enable the formulation of diets that more closely meet amino acid requirements. In vitro and in vivo techniques ...
... The cost of feed represents >65% of the bird arriving at a US processing plant (July 2005 Agri Stats); therefore a more precise knowledge of the nutritional value of feed ingredients would enable the formulation of diets that more closely meet amino acid requirements. In vitro and in vivo techniques ...
Molecular networks in skeletal muscle plasticity
... PGC-1α is considered to be the key downstream element of the signaling cascades activated by endurance exercise in muscle tissue (Chan and Arany, 2014) (Fig. 1). The PGC-1α gene is controlled by several promoters, coupled to alternative splicing, involved in activating gene programs for adaptations ...
... PGC-1α is considered to be the key downstream element of the signaling cascades activated by endurance exercise in muscle tissue (Chan and Arany, 2014) (Fig. 1). The PGC-1α gene is controlled by several promoters, coupled to alternative splicing, involved in activating gene programs for adaptations ...
Advanced Stochastic Protein Sequence Analysis
... to traditional research in molecular biology, i.e. explorations in the so-called wet labs, insilico investigations are mostly cheaper and faster by some orders of magnitude. Here, the term in-silico stands for experiments using bioinformatics methods on computers. Thus, contrary to traditional resea ...
... to traditional research in molecular biology, i.e. explorations in the so-called wet labs, insilico investigations are mostly cheaper and faster by some orders of magnitude. Here, the term in-silico stands for experiments using bioinformatics methods on computers. Thus, contrary to traditional resea ...
University of Groningen Stress responses and sugar metabolism in
... In addition to these phosphoproteins also low molecular weight molecules such as NADP, glucose‐6‐phosphate (G6P) and fructose‐1,6‐bisphosphate (FBP) can modulate either DNA‐ binding properties or the interaction with the transcriptional machinery of the CcpA‐(HPr‐ Ser ...
... In addition to these phosphoproteins also low molecular weight molecules such as NADP, glucose‐6‐phosphate (G6P) and fructose‐1,6‐bisphosphate (FBP) can modulate either DNA‐ binding properties or the interaction with the transcriptional machinery of the CcpA‐(HPr‐ Ser ...
Identification and characterization of novel interaction
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
International Journal on New Trends in Education and Their
... stopper codon. mRNA slips. tRNAs are synthesized through mRNAs. Afterwards, a peptide bond is created between tRNAs. Considering the expression of the pre-service teacher, it can be inferred that he/she assumes tRNAs are interconnected via peptide bonds. CONCLUSIONS AND DISCUSSION In consequence of ...
... stopper codon. mRNA slips. tRNAs are synthesized through mRNAs. Afterwards, a peptide bond is created between tRNAs. Considering the expression of the pre-service teacher, it can be inferred that he/she assumes tRNAs are interconnected via peptide bonds. CONCLUSIONS AND DISCUSSION In consequence of ...
Purification of Arrestin from Bovine Retinas
... It has been reported that bovine arrestin may contain lipids and carbohydrate moieties, covalently attached to the protein (for review, see Ref. 22). It was also reported that arrestin can be modified substoichiometrically by phosphorylation, presumably by protein kinase C (23). Mass spectroscopic a ...
... It has been reported that bovine arrestin may contain lipids and carbohydrate moieties, covalently attached to the protein (for review, see Ref. 22). It was also reported that arrestin can be modified substoichiometrically by phosphorylation, presumably by protein kinase C (23). Mass spectroscopic a ...
Gene Section CDC25A (cell division cycle 25A) Atlas of Genetics and Cytogenetics
... The other reported isoform CDC25A2, consists of 484 amino acids with a molecular weight of 54.4 kDa. Both the isoforms have the same N- and C-terminal end, thus expected to have similar catalytic activity. The Nterminal regulatory domain contains several phosphorylation sites and show low sequence h ...
... The other reported isoform CDC25A2, consists of 484 amino acids with a molecular weight of 54.4 kDa. Both the isoforms have the same N- and C-terminal end, thus expected to have similar catalytic activity. The Nterminal regulatory domain contains several phosphorylation sites and show low sequence h ...
BCHM 2300 Test III - Lipids and Metabolism
... 60. True or False? If both nutrients are provided in diet, the body will utilize proteins prior to carbohydrates as a source of energy. A) True B) False 61. True or False? The body typically produces nine essential amino acids. A) True B) False 62. True or False? Proteins play a major role in the im ...
... 60. True or False? If both nutrients are provided in diet, the body will utilize proteins prior to carbohydrates as a source of energy. A) True B) False 61. True or False? The body typically produces nine essential amino acids. A) True B) False 62. True or False? Proteins play a major role in the im ...
Structure of a glutamate transporter homologue from Pyrococcus
... Glutamate transporters are integral membrane proteins that catalyse the concentrative uptake of glutamate from the synapse to intracellular spaces by harnessing pre-existing ion gradients. In the central nervous system glutamate transporters are essential for normal development and function, and are ...
... Glutamate transporters are integral membrane proteins that catalyse the concentrative uptake of glutamate from the synapse to intracellular spaces by harnessing pre-existing ion gradients. In the central nervous system glutamate transporters are essential for normal development and function, and are ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.