Investigating The Metal Binding Sites In Znta, A Zinc Transporting
... Sensitivity to metal salts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60 ...
... Sensitivity to metal salts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60 ...
PDH02 - OSU Biochemistry and Molecular Biology
... N-terminal tails that intertwin in a firm handshake whereas the P. putida structure extents to opposite sides on the tetramer far from crossing paths. ...
... N-terminal tails that intertwin in a firm handshake whereas the P. putida structure extents to opposite sides on the tetramer far from crossing paths. ...
Protein translation in Plasmodium parasites
... tRNAs, ribosomal proteins, and the translation elongation factor Tu (EF-Tu), and also a handful of other poorly characterized proteins unrelated to translation (Figure 1). Most proteins resident in the apicoplast are encoded by the nuclear genome, translated in the cytoplasm, and transported into th ...
... tRNAs, ribosomal proteins, and the translation elongation factor Tu (EF-Tu), and also a handful of other poorly characterized proteins unrelated to translation (Figure 1). Most proteins resident in the apicoplast are encoded by the nuclear genome, translated in the cytoplasm, and transported into th ...
Chromatin Condensing Functions of the Linker Histone C
... linker histone-containing chromatin fibers was confirmed by sedimentation velocity in TEN. Sedimentation Velocity. Sedimentation velocity experiments were performed in a Beckman XLA or XLI ultracentrifuge equipped with scanner optics as described (7, 18, 34). Scans were analyzed by the modified meth ...
... linker histone-containing chromatin fibers was confirmed by sedimentation velocity in TEN. Sedimentation Velocity. Sedimentation velocity experiments were performed in a Beckman XLA or XLI ultracentrifuge equipped with scanner optics as described (7, 18, 34). Scans were analyzed by the modified meth ...
Physiology of a New Facultatively Autotrophic
... and growth was followed turbidimetrically using a Klett-Summerson colorimeter with a red filter (transmission 640 to 700 nm). This method of estimating growth is prone to errors due to variation in cell size and shape and the organism described here tended to grow in strands, which complicated the d ...
... and growth was followed turbidimetrically using a Klett-Summerson colorimeter with a red filter (transmission 640 to 700 nm). This method of estimating growth is prone to errors due to variation in cell size and shape and the organism described here tended to grow in strands, which complicated the d ...
Drought response in the spikes of barley: gene expression in the
... constant variance from block to block. The significance of differential gene expression in stressed and non-stressed organs was determined using the F statistic. To control false discoveries due to multiple hypotheses testing, we used the q value method described by Storey and Tibshirani (2003). Pro ...
... constant variance from block to block. The significance of differential gene expression in stressed and non-stressed organs was determined using the F statistic. To control false discoveries due to multiple hypotheses testing, we used the q value method described by Storey and Tibshirani (2003). Pro ...
Creation/Evolution - Geoscience Research Institute
... Are Codons The Language of God? The genetic code appears to be non-random in nature and designed with considerable safeguards against harmful point mutations An evolutionary model suggests at least at some level of randomness in assignment of amino acids to codons No mechanism exists for genetic cod ...
... Are Codons The Language of God? The genetic code appears to be non-random in nature and designed with considerable safeguards against harmful point mutations An evolutionary model suggests at least at some level of randomness in assignment of amino acids to codons No mechanism exists for genetic cod ...
Two Arabidopsis Genes (IPMS1 and IPMS2
... Heterologous expression of the Arabidopsis (Arabidopsis thaliana) IPMS1 (At1g18500) and IPMS2 (At1g74040) cDNAs in Escherichia coli yields isopropylmalate synthases (IPMSs; EC 2.3.3.13). These enzymes catalyze the first dedicated step in leucine (Leu) biosynthesis, an aldol-type condensation of acet ...
... Heterologous expression of the Arabidopsis (Arabidopsis thaliana) IPMS1 (At1g18500) and IPMS2 (At1g74040) cDNAs in Escherichia coli yields isopropylmalate synthases (IPMSs; EC 2.3.3.13). These enzymes catalyze the first dedicated step in leucine (Leu) biosynthesis, an aldol-type condensation of acet ...
Metabolic decision making by protein-metabolite - ETH E
... network, on central metabolic fluxes, and discuss the trade‐off between efficient metabolic operation in a given environment and resilience against inevitable internal and external fluctuations. In chapter 3, we develop a combined experimental‐computational approach to dissect the cont ...
... network, on central metabolic fluxes, and discuss the trade‐off between efficient metabolic operation in a given environment and resilience against inevitable internal and external fluctuations. In chapter 3, we develop a combined experimental‐computational approach to dissect the cont ...
CP1 Domain in Escherichia coli Leucyl
... steps; amino acids larger than the cognate substrate are rejected by a “coarse sieve”, while the reaction products of amino acids smaller than the cognate substrate will go through a “fine sieve” and be hydrolyzed. This “double-sieve” mechanism has been proposed for IleRS, a class I aminoacyl-tRNA s ...
... steps; amino acids larger than the cognate substrate are rejected by a “coarse sieve”, while the reaction products of amino acids smaller than the cognate substrate will go through a “fine sieve” and be hydrolyzed. This “double-sieve” mechanism has been proposed for IleRS, a class I aminoacyl-tRNA s ...
USGENE - Chemical Abstracts Service
... The GETSIM and BLAST run packages are available to search the USGENE database for protein and nucleotide sequence data by similarity (homology). BLAST is provided in USGENE with the permission of the National Center for Biotechnology Information (NCBI) of the National Library of Medicine (NLM). GETS ...
... The GETSIM and BLAST run packages are available to search the USGENE database for protein and nucleotide sequence data by similarity (homology). BLAST is provided in USGENE with the permission of the National Center for Biotechnology Information (NCBI) of the National Library of Medicine (NLM). GETS ...
Arabidopsis Contains Nine Long-Chain Acyl
... effort to better understand the factors that control fatty acid metabolism in oilseeds, we have sought to identify and characterize genes that encode LACSs in Arabidopsis. Nine cDNAs were identified, cloned, and tested for their ability to complement a LACS-deficient strain of yeast (Saccharomyces c ...
... effort to better understand the factors that control fatty acid metabolism in oilseeds, we have sought to identify and characterize genes that encode LACSs in Arabidopsis. Nine cDNAs were identified, cloned, and tested for their ability to complement a LACS-deficient strain of yeast (Saccharomyces c ...
NTPase/helicase of Flaviviridae: inhibitors and inhibition of the
... SF2 NTPase/helicases are further divided, according to the sequence surrounding the conserved D-E residues (Walker motif B) in three different subgroups of proteins. The first is formed by the classic D-E-A-D box proteins, and the other are named D-E-A-H and D-E-X-H, based on their deviating Walker ...
... SF2 NTPase/helicases are further divided, according to the sequence surrounding the conserved D-E residues (Walker motif B) in three different subgroups of proteins. The first is formed by the classic D-E-A-D box proteins, and the other are named D-E-A-H and D-E-X-H, based on their deviating Walker ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporters* J. Dairy Sci. 88:182–194
... values normalized to the housekeeping gene, β-actin. Statistical significance of relative differences in mRNA expression was tested using the Mixed Procedure of SAS (version 8.2; SAS Institute, Inc., Cary, NC) to account for repeated measures. The model included biopsy day as a fixed effect and cow ...
... values normalized to the housekeeping gene, β-actin. Statistical significance of relative differences in mRNA expression was tested using the Mixed Procedure of SAS (version 8.2; SAS Institute, Inc., Cary, NC) to account for repeated measures. The model included biopsy day as a fixed effect and cow ...
Trade-offs between tRNA abundance and mRNA secondary
... choice and mRNA secondary structure affected translation, we used absolute expression data from a library of 29 DNA polymerase synonymous codon variants (36). In total, this contained 39 gene variants of which 30 exhibited measurable expression. Hence, only these 30 variants were included in our an ...
... choice and mRNA secondary structure affected translation, we used absolute expression data from a library of 29 DNA polymerase synonymous codon variants (36). In total, this contained 39 gene variants of which 30 exhibited measurable expression. Hence, only these 30 variants were included in our an ...
Unveiling the physiological and molecular basis of Mycobacterium
... bovis under the presence of rifampicin 25 µg/ml decreased survival percentage to 50%, although the observed effect in growth rate was less pronounced than the one induced by low pH. Mycobacterial cells formed reticulated pellicles at the air-medium interface and attached to the periphery of 12 well- ...
... bovis under the presence of rifampicin 25 µg/ml decreased survival percentage to 50%, although the observed effect in growth rate was less pronounced than the one induced by low pH. Mycobacterial cells formed reticulated pellicles at the air-medium interface and attached to the periphery of 12 well- ...
(lip) that - Repositories
... monoxygenases, but using sulfur rather than oxygen, would also achieve the desired result. Knight et al. (1963) showed that sulfur could be directly inserted into C-H bonds of hydrocarbons (White, 1980b). However, most monoxygenase-catalyzed hydroxylations proceed with retention of configuration, bu ...
... monoxygenases, but using sulfur rather than oxygen, would also achieve the desired result. Knight et al. (1963) showed that sulfur could be directly inserted into C-H bonds of hydrocarbons (White, 1980b). However, most monoxygenase-catalyzed hydroxylations proceed with retention of configuration, bu ...
Conformational Preferences of Amino Acids in Globular Proteins?
... (a) two proteins are the independentlv solved halves of a dimer. (b) the same protein was solved by hifferent groups, and (c) two or more proteins are closely related and have homologous sequences. Rather than choose one representative protein from each redundant class, all the available data was us ...
... (a) two proteins are the independentlv solved halves of a dimer. (b) the same protein was solved by hifferent groups, and (c) two or more proteins are closely related and have homologous sequences. Rather than choose one representative protein from each redundant class, all the available data was us ...
Differential Regulation of a-Lactalbumin and
... tating Glands.Total mRNAwasisolatedfrom rat mammary control of casein mRNA is suggested by removal of the gland on various days of pregnancy and lactation. Chart 8 pups during lactation; casein messenger levels are dimin shows total protein synthesized as a function of the pg of ished (Chart 9A). Wh ...
... tating Glands.Total mRNAwasisolatedfrom rat mammary control of casein mRNA is suggested by removal of the gland on various days of pregnancy and lactation. Chart 8 pups during lactation; casein messenger levels are dimin shows total protein synthesized as a function of the pg of ished (Chart 9A). Wh ...
Ovid: Parenteral administration of different amounts of branch
... Objective: To study the effects of a total parenteral nutrition solution changing branch-chain amino acid concentrations and/or nitrogen supply on protein metabolism, length of stay, and mortality rate; and to evaluate the unique metabolic status of sepsis that leads to a search for specific total p ...
... Objective: To study the effects of a total parenteral nutrition solution changing branch-chain amino acid concentrations and/or nitrogen supply on protein metabolism, length of stay, and mortality rate; and to evaluate the unique metabolic status of sepsis that leads to a search for specific total p ...
- BioMedSearch
... The purified PSRP catalysed both the ADP/ATP-dependent inactivation (Table 1) and the Pi-dependent activation of purified E. coli PEPS (Table 2). In addition, the expressed PSRP catalysed in-assay activation. In-assay activation occurs when inactivated PEPS in aliquots taken from a separate inactiva ...
... The purified PSRP catalysed both the ADP/ATP-dependent inactivation (Table 1) and the Pi-dependent activation of purified E. coli PEPS (Table 2). In addition, the expressed PSRP catalysed in-assay activation. In-assay activation occurs when inactivated PEPS in aliquots taken from a separate inactiva ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.