Profile TildeCRF: a new tool for protein homology detection
... It has been over 150 years since the outstanding scientific discoveries that laid the foundations for modern biology and genetics. Charles Darwin described the mechanism of natural selection, whereby individuals that are best adapted to their environment are more likely to survive and reproduce, pas ...
... It has been over 150 years since the outstanding scientific discoveries that laid the foundations for modern biology and genetics. Charles Darwin described the mechanism of natural selection, whereby individuals that are best adapted to their environment are more likely to survive and reproduce, pas ...
Host Factors in the Replication of Positive
... replication process of (+)RNA viruses is complex, involving not only interactions of viral RNAs and viral proteins but also their interplay with host factors. Studies performed for infections with HCV, DENV, WNV, and several plant (+)RNA viruses have shown that viral RNA replication requires host me ...
... replication process of (+)RNA viruses is complex, involving not only interactions of viral RNAs and viral proteins but also their interplay with host factors. Studies performed for infections with HCV, DENV, WNV, and several plant (+)RNA viruses have shown that viral RNA replication requires host me ...
Evaluation of Corn Distillers Grains and Ruminally Protected Lysine
... cows were fed SBM. Increased milk protein percentages and yields have occurred in several studies in which cows were fed RPLM (7, 19, 20, 22). Body weight and body condition scores were unaffected by protein source or RPLM, which suggests that all diets were adequate for supporting the nutrient requ ...
... cows were fed SBM. Increased milk protein percentages and yields have occurred in several studies in which cows were fed RPLM (7, 19, 20, 22). Body weight and body condition scores were unaffected by protein source or RPLM, which suggests that all diets were adequate for supporting the nutrient requ ...
Invariant amino acids essential for decoding function of polypeptide
... activities are not affected at all and the GTPase activity of eRF3 which is entirely dependent on the binding of eRF1 and eRF3 to the ribosome as mentioned above remains as in the presence of the wild-type eRF1 (Figure 2). Most likely, invariant Tyr125 is essential for the UAG stop codon recognition ...
... activities are not affected at all and the GTPase activity of eRF3 which is entirely dependent on the binding of eRF1 and eRF3 to the ribosome as mentioned above remains as in the presence of the wild-type eRF1 (Figure 2). Most likely, invariant Tyr125 is essential for the UAG stop codon recognition ...
An Investigation of Acetobacter aceti N5
... mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was found to be significantly more thermostable than EcPurE over the entire pH range surve ...
... mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was found to be significantly more thermostable than EcPurE over the entire pH range surve ...
As a PDF file
... in LAB. The second part describes isolation, cloning and characterization of genes encoding enzymes that are promising for the further development of genetically modified lactic acid bacteria. In the first part, either the ldhD and ldhL genes, or ldhD alone, were inactivated using gene replacement t ...
... in LAB. The second part describes isolation, cloning and characterization of genes encoding enzymes that are promising for the further development of genetically modified lactic acid bacteria. In the first part, either the ldhD and ldhL genes, or ldhD alone, were inactivated using gene replacement t ...
Malate Dehydrogenases – Structure and Function
... The amino acid sequences of MDHs show divergence into two main phylogenetic groups of closely related enzymes. MDHs from some eubacteria (e.g. Escherichia coli) have relatively high sequence identity with the mitochondrial enzymes of eukaryotes, whereas other eubacterial MDHs (e.g. Thermus flavus) a ...
... The amino acid sequences of MDHs show divergence into two main phylogenetic groups of closely related enzymes. MDHs from some eubacteria (e.g. Escherichia coli) have relatively high sequence identity with the mitochondrial enzymes of eukaryotes, whereas other eubacterial MDHs (e.g. Thermus flavus) a ...
Marine alga Sargassum horneri active component
... an important role in basic biological functions, such as intracellular clearance of defective proteins and organelles, differentiation, and development [3-5]. Dysfunctions in autophagy are associated with severe diseases, such as heart disease, neurodegenerative disorders, and cancers [4,6,7]. There ...
... an important role in basic biological functions, such as intracellular clearance of defective proteins and organelles, differentiation, and development [3-5]. Dysfunctions in autophagy are associated with severe diseases, such as heart disease, neurodegenerative disorders, and cancers [4,6,7]. There ...
Supplementary materials - Stanford Artificial Intelligence Laboratory
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
Using antibody catalysis to study the outcome of multiple
... and the light chains were expressed from two CMV promoters to give an IgG1 hybrid antibody with a human kappa constant region and human ␥1 constant region (C.R. and C.F.B., unpublished data). The antibodies were expressed in a human cell line and were secreted into the cell culture medium, from whic ...
... and the light chains were expressed from two CMV promoters to give an IgG1 hybrid antibody with a human kappa constant region and human ␥1 constant region (C.R. and C.F.B., unpublished data). The antibodies were expressed in a human cell line and were secreted into the cell culture medium, from whic ...
Protein Profile Characterization of Donkey Milk
... if compared with cows milk. The high content is responsible for the good palatability and facilitates the intestinal absorption of calcium that is essential for infant’s bone mineralization. Donkey milk shows a lower fat content compared to human milk, presenting for this reason a reduced energetic ...
... if compared with cows milk. The high content is responsible for the good palatability and facilitates the intestinal absorption of calcium that is essential for infant’s bone mineralization. Donkey milk shows a lower fat content compared to human milk, presenting for this reason a reduced energetic ...
thèse - Université Evry Val d`Essonne
... proteins. mtDNA generally encodes catalytic subunits of the complexes of the respiratory chain with tRNA and rRNA components of the mitochondrial translation system, but it can also contain additional proteins; in S. cerevisiae, besides seven subunits of complexes involved in oxidative phosphorylati ...
... proteins. mtDNA generally encodes catalytic subunits of the complexes of the respiratory chain with tRNA and rRNA components of the mitochondrial translation system, but it can also contain additional proteins; in S. cerevisiae, besides seven subunits of complexes involved in oxidative phosphorylati ...
Biotechnology and Bioengineering
... and a 957 bp DNA fragment that includes the SalI restriction site downstream of codon F176 was amplified using primers F176-front and ToMO-SalI-rear (Table I). Similarly, to perform saturation mutagenesis at TouA position F196, a 763 bp DNA fragment was amplified using primers ToMO-KpnI-front and F1 ...
... and a 957 bp DNA fragment that includes the SalI restriction site downstream of codon F176 was amplified using primers F176-front and ToMO-SalI-rear (Table I). Similarly, to perform saturation mutagenesis at TouA position F196, a 763 bp DNA fragment was amplified using primers ToMO-KpnI-front and F1 ...
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... myeloid cells. 5-‐LO converts AA to LTA4 in cooperation with 5-‐LO activating protein (FLAP). LTA4 is converted to LTB4, by LTA4-‐hydrolase (LTA4H) or to LTC4 by LTC4-‐synthase (LTC4S). LTs act on cell ...
... myeloid cells. 5-‐LO converts AA to LTA4 in cooperation with 5-‐LO activating protein (FLAP). LTA4 is converted to LTB4, by LTA4-‐hydrolase (LTA4H) or to LTC4 by LTC4-‐synthase (LTC4S). LTs act on cell ...
Interaction of Urea with Amino Acids: Implications for Urea
... each of 100 ns length. The initial backbone angles of the tripeptides were set in β-sheet conformation (φ ) -135°, ψ ) 128°). Since the autocorrelation time of (φ, ψ) was found to be below 1 ns in our simulations, these starting conditions did not impose significant bias. All tripeptides were simula ...
... each of 100 ns length. The initial backbone angles of the tripeptides were set in β-sheet conformation (φ ) -135°, ψ ) 128°). Since the autocorrelation time of (φ, ψ) was found to be below 1 ns in our simulations, these starting conditions did not impose significant bias. All tripeptides were simula ...
Amino Acid Transporters and Release of Hydrophobic Amino Acids
... uptake was determined as described previously [23] in 10-min transport assays with the indicated concentration of L-[U-14C]amino acid, in filaments grown in BG11 medium (supplemented with the appropriate antibiotic in the case of the mutants), washed and suspended in 25 mM N-tris(hydroxymethyl)-meth ...
... uptake was determined as described previously [23] in 10-min transport assays with the indicated concentration of L-[U-14C]amino acid, in filaments grown in BG11 medium (supplemented with the appropriate antibiotic in the case of the mutants), washed and suspended in 25 mM N-tris(hydroxymethyl)-meth ...
Altered Fermentative Metabolism in
... acetyl-CoA produced either via the PFL1 or PFR1 pathways enters the acetate-generating phosphate acetyltransferase-acetate kinase (PAT1/PAT2ACK2/ACK1) or the bifunctional ADH1 pathways. PFR1 has a proposed role in dark, fermentative H2 production, because it reduces ferredoxin (FD), which in turn pr ...
... acetyl-CoA produced either via the PFL1 or PFR1 pathways enters the acetate-generating phosphate acetyltransferase-acetate kinase (PAT1/PAT2ACK2/ACK1) or the bifunctional ADH1 pathways. PFR1 has a proposed role in dark, fermentative H2 production, because it reduces ferredoxin (FD), which in turn pr ...
- Wiley Online Library
... operates in B. subtilis and which governs the expression of genes encoding the assimilatory nitrate reductase (see below). More intensive and systematic studies have been undertaken over the past 3 years to examine the ability of B. subtilis cells to grow anaerobically. Most, if not all, studies wer ...
... operates in B. subtilis and which governs the expression of genes encoding the assimilatory nitrate reductase (see below). More intensive and systematic studies have been undertaken over the past 3 years to examine the ability of B. subtilis cells to grow anaerobically. Most, if not all, studies wer ...
Steroid Chemistry and Steroid Hormone Action - Rose
... The steroid hormone receptors are generally present in small quantities (a few hundred to a few thousand molecules per cell). They have high affinity for their ligands (dissociation constant usually less than 1 nanomolar). They function as ligand activated transcription factors, specifically activat ...
... The steroid hormone receptors are generally present in small quantities (a few hundred to a few thousand molecules per cell). They have high affinity for their ligands (dissociation constant usually less than 1 nanomolar). They function as ligand activated transcription factors, specifically activat ...
Assigned Reading
... The central paradigm driving research in molecular biology has been that biological information flows from DNA to RNA to protein. The power of this concept lies in its templatebased precision, the ability to manipulate one class of molecules based on knowledge of another, and the patterns of sequen ...
... The central paradigm driving research in molecular biology has been that biological information flows from DNA to RNA to protein. The power of this concept lies in its templatebased precision, the ability to manipulate one class of molecules based on knowledge of another, and the patterns of sequen ...
Quantitative Comparison of the Hydrogen Bond
... segments are connected by flexible linkers. The N-H order parameters S2 for most amino acids in all three segments have values (∼0.4-0.6) that are substantially lower than in folded globular proteins, and significant amounts of internal ...
... segments are connected by flexible linkers. The N-H order parameters S2 for most amino acids in all three segments have values (∼0.4-0.6) that are substantially lower than in folded globular proteins, and significant amounts of internal ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.