ANP 204 Main Text - National Open University of Nigeria
... glucose but for a change in the positions of OH group on carbon 2 and 4 for mannose and galactose respectively. The carbon atoms involved are ...
... glucose but for a change in the positions of OH group on carbon 2 and 4 for mannose and galactose respectively. The carbon atoms involved are ...
A model for mis-sense error in protein synthesis: mis
... cognate tRNA for a given codon, such a mis-charged tRNA compromises the translational fidelity by contributing an amino acid which is different from that dictated by the mRNA template. Erroneous substitution of one amino acid by another is called mis-sense error. Pre-translational mis-charging of tR ...
... cognate tRNA for a given codon, such a mis-charged tRNA compromises the translational fidelity by contributing an amino acid which is different from that dictated by the mRNA template. Erroneous substitution of one amino acid by another is called mis-sense error. Pre-translational mis-charging of tR ...
Transcription factor Sp3 is silenced through - IMT
... the ID of Sp3, a yeast two-hybrid screen with the ID of Sp3 fused to the LexA DNA-binding domain (LexA-IDSp3) had been performed. A single clone interacted exclusively with the LexA-IDSp3 wild-type protein but not with LexA on its own or with a mutant in which the KEE sequence of the SUMO motif was ...
... the ID of Sp3, a yeast two-hybrid screen with the ID of Sp3 fused to the LexA DNA-binding domain (LexA-IDSp3) had been performed. A single clone interacted exclusively with the LexA-IDSp3 wild-type protein but not with LexA on its own or with a mutant in which the KEE sequence of the SUMO motif was ...
... crops and agricultural residues) and to increase productivity, robustness, and product yield (for reviews see references 20 and 35). A major challenge relating to the stoichiometry of yeast-based ethanol production is that substantial amounts of glycerol are invariably formed as a by-product (24). I ...
The DnaE polymerase from Deinococcus radiodurans features
... interacts with the core α subunit. The β-clamp is a homodimer, and confers to the holoenzyme high processivity by tethering the polymerase to the DNA substrate [20, 21]. In addition, the progression of a replication fork is assisted by the association of DNA Pol III with DNA helicase and primase [22 ...
... interacts with the core α subunit. The β-clamp is a homodimer, and confers to the holoenzyme high processivity by tethering the polymerase to the DNA substrate [20, 21]. In addition, the progression of a replication fork is assisted by the association of DNA Pol III with DNA helicase and primase [22 ...
Comparative transcriptomics reveals key gene expression
... inside the host. We therefore also chose to study the effect of acid-shock on the steady-state transcriptomes of M. tuberculosis and M. bovis as a means to probe the response of both strains to a perturbation. Hence, the aim of this study was to identify key gene expression differences between M. tu ...
... inside the host. We therefore also chose to study the effect of acid-shock on the steady-state transcriptomes of M. tuberculosis and M. bovis as a means to probe the response of both strains to a perturbation. Hence, the aim of this study was to identify key gene expression differences between M. tu ...
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional
... bootstrap analysis with 500 replicates; the proportion of invariable sites was evaluated during the analysis, whereas the range of rate variation across site was determined by gamma distribution set to 5 number of categories. A Bayesian Markov Chain Monte Carlo (MCMC) analysis was also performed by ...
... bootstrap analysis with 500 replicates; the proportion of invariable sites was evaluated during the analysis, whereas the range of rate variation across site was determined by gamma distribution set to 5 number of categories. A Bayesian Markov Chain Monte Carlo (MCMC) analysis was also performed by ...
Bacterial Survival in Suspension in Polyethylene Glycol Solutions
... containing 0.004 M-potassium cyanide added to prevent phage development. After 2 rnin, to allow the cyanide to take effect, phage T 7 was added and the mixture maintained a t 37" for a further period of 10 min. to allow adsorption to occur. After centrifugation, which left unadsorbed phage in the su ...
... containing 0.004 M-potassium cyanide added to prevent phage development. After 2 rnin, to allow the cyanide to take effect, phage T 7 was added and the mixture maintained a t 37" for a further period of 10 min. to allow adsorption to occur. After centrifugation, which left unadsorbed phage in the su ...
Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant
... For product profile analysis, CE (capillary electrophoresis) was performed using similar conditions to those developed for the separation of heparan sulphate GAG disaccharides [18]. Substrates used included C6S from shark cartilage (Sigma), DS from porcine intestinal mucosa (Sigma) and C4S from stur ...
... For product profile analysis, CE (capillary electrophoresis) was performed using similar conditions to those developed for the separation of heparan sulphate GAG disaccharides [18]. Substrates used included C6S from shark cartilage (Sigma), DS from porcine intestinal mucosa (Sigma) and C4S from stur ...
The Ovine Lens Cytoskeleton - Lincoln University Research Archive
... Intermediate filament proteins range in molecular weight from 45 to 150 kDa, and share a common structure based on the presence of non-!-helical N- and C-terminal domain ends and a central !-helical rod domain that is well conserved in size and predicted secondary structure (Alizadeh et al., 2002). ...
... Intermediate filament proteins range in molecular weight from 45 to 150 kDa, and share a common structure based on the presence of non-!-helical N- and C-terminal domain ends and a central !-helical rod domain that is well conserved in size and predicted secondary structure (Alizadeh et al., 2002). ...
Are You suprised ?
... organisms that appear to have few physical similarities may have similar sequences of amino acids in their proteins and be closely related through evolution. Many researchers believe that the greater the similarity in the amino-acid sequences of two organisms, the more closely related they are in an ...
... organisms that appear to have few physical similarities may have similar sequences of amino acids in their proteins and be closely related through evolution. Many researchers believe that the greater the similarity in the amino-acid sequences of two organisms, the more closely related they are in an ...
Attachment 1 - Food Standards Australia New Zealand
... potential costs and benefits of the proposed change to the Code from stakeholders is highly desirable. Claims made in submissions should be supported wherever possible by referencing or including relevant studies, research findings, trials, surveys etc. Technical information should be in sufficient ...
... potential costs and benefits of the proposed change to the Code from stakeholders is highly desirable. Claims made in submissions should be supported wherever possible by referencing or including relevant studies, research findings, trials, surveys etc. Technical information should be in sufficient ...
Mycobacterium tuberculosis DNA gyrase ATPase domain structures
... its ATPase activity using a sensitive fluorescence assay which measures the production of Pi [28]. As the ATPase activity of the isolated ATPase domain is quite low, 15 μM protein was used. Figure 1 shows an ATP titration for the ATPase domain. An ATP-dependent increase in activity was observed, and ...
... its ATPase activity using a sensitive fluorescence assay which measures the production of Pi [28]. As the ATPase activity of the isolated ATPase domain is quite low, 15 μM protein was used. Figure 1 shows an ATP titration for the ATPase domain. An ATP-dependent increase in activity was observed, and ...
INTERACTION STUDY OF CURCULIGOSIDE A AND ITS AGLYCONE AS CYCLOOXYGENASE
... irritants [1]. Inflammation is a basic way in which the body reacts to infection, irritation or other injury. The key features are redness, warmth, swelling and pain. Inflammation is now recognized as a type of non-specific immune response [2]. Cyclooxygenase (COX) is an enzyme involved in the synth ...
... irritants [1]. Inflammation is a basic way in which the body reacts to infection, irritation or other injury. The key features are redness, warmth, swelling and pain. Inflammation is now recognized as a type of non-specific immune response [2]. Cyclooxygenase (COX) is an enzyme involved in the synth ...
Enzyme Mechanisms - Illinois Institute of Technology
... unrelated settings Subtilisin: externals very different from mammalian serine proteases; triad same ...
... unrelated settings Subtilisin: externals very different from mammalian serine proteases; triad same ...
Unnatural Amino Acid Mutagenesis of the GABAA Receptor Binding
... values, GABA concentration–response data were fitted using PRISM software (GraphPad, San Diego, CA) to the four-parameter logistic equan tion, Y ⫽ Imin ⫹ (Imax ⫺ Imin)/1 ⫹ 10(logEC50 ⫺ [A]) H, where Imax is the maximal peak current and nH is the Hill coefficient, and with the concentration values en ...
... values, GABA concentration–response data were fitted using PRISM software (GraphPad, San Diego, CA) to the four-parameter logistic equan tion, Y ⫽ Imin ⫹ (Imax ⫺ Imin)/1 ⫹ 10(logEC50 ⫺ [A]) H, where Imax is the maximal peak current and nH is the Hill coefficient, and with the concentration values en ...
Metabolic significance of inorganic triphosphate, thiamine
... specific for PPPi plays only a minor role in the cytosolic PPPase activity, while most of the activity is due to inorganic pyrophosphatase. In animal tissues, most PPPase activity is due to the short-chain exopolyphosphatase prune, which hydrolyzes PPPi with high catalytic efficiency. We hypothesize ...
... specific for PPPi plays only a minor role in the cytosolic PPPase activity, while most of the activity is due to inorganic pyrophosphatase. In animal tissues, most PPPase activity is due to the short-chain exopolyphosphatase prune, which hydrolyzes PPPi with high catalytic efficiency. We hypothesize ...
Amino acid analysis in biofluids using LC
... valine (Val). Arg, Cys and Tyr are semi-essential and need to provided by food during early childhood (3). Semi-essential AA can be synthesized by the human body but under certain conditions (growth, strong physical strain) a number of AA are essential and must be taken by food. AA play an important ...
... valine (Val). Arg, Cys and Tyr are semi-essential and need to provided by food during early childhood (3). Semi-essential AA can be synthesized by the human body but under certain conditions (growth, strong physical strain) a number of AA are essential and must be taken by food. AA play an important ...
Isolation of Rat Transferrin Using CNBr-Activated
... iron containing proteins transferrin and ferritin in a homogeneous state. Transferrin is the most important transport protein in serum due to its quantity and biological role in the transport of iron to the haemopoietic system. For experimental work on model systems in animals we need purified trans ...
... iron containing proteins transferrin and ferritin in a homogeneous state. Transferrin is the most important transport protein in serum due to its quantity and biological role in the transport of iron to the haemopoietic system. For experimental work on model systems in animals we need purified trans ...
Altered pathogenicity, immunogenicity, tissue tropism and 3`
... for titrations. At each dilution, five embryos received 0.1 mL inoculum. The embryos were candled daily and examined for one week; those showing characteristic IBV lesions, such as dwarfing, stunting, or curling of embryos, were recorded as infected by IBV. The 10 swab samples taken in the pathogeni ...
... for titrations. At each dilution, five embryos received 0.1 mL inoculum. The embryos were candled daily and examined for one week; those showing characteristic IBV lesions, such as dwarfing, stunting, or curling of embryos, were recorded as infected by IBV. The 10 swab samples taken in the pathogeni ...
Engineering Cytosolic Acetyl-CoA Metabolism in Saccharomyces
... In connection with biobased chemical production, it is necessary to engineer the metabolism of cell factories such that the raw material, typically sugars, can be efficiently converted to the product of interest. Although IMI076 could grow on glucose, it was still inefficient at conversion of pyruva ...
... In connection with biobased chemical production, it is necessary to engineer the metabolism of cell factories such that the raw material, typically sugars, can be efficiently converted to the product of interest. Although IMI076 could grow on glucose, it was still inefficient at conversion of pyruva ...
Structural and Biochemical Characterization of a Bifunctional
... polypeptide chains. This is not the case for other organisms, however. Take, for example, Shewanella denitrif icans, which is a Gram-negative bacterium first isolated from the Baltic Sea.7 In this organism, a gene has been identified that apparently encodes for a bifunctional protein with both 3,4-ket ...
... polypeptide chains. This is not the case for other organisms, however. Take, for example, Shewanella denitrif icans, which is a Gram-negative bacterium first isolated from the Baltic Sea.7 In this organism, a gene has been identified that apparently encodes for a bifunctional protein with both 3,4-ket ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.