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Final Report
Final Report

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1 BIOL 213 Second Exam All atoms, chemical bonding and
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... proteins come in two forms: complete proteins contain all eight of the amino acids (threonine, valine, tryptophan, isoleucine, leucine, lysine, phenylalanine, and methionine) that humans cannot produce themselves, while incomplete proteins lack or contain only a very small proportion of one or more ...
Slide 1
Slide 1

... ...
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File - Mrs. Badger`s Honors Biology Class

... _____ 1. Choose the nucleotide sequence of the RNA strand that would be complementary to the following DNA strand: GTAGTCA a. UATUAGA. b. ACGACTG. c. CAUCAGU. d. CATCAGT. _____ 2. The main function of tRNA is to a. carry a message that, when translated, forms proteins. b. form a portion of ribosomes ...
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< 1 ... 462 463 464 465 466

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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